Immunocytochemical labeling of neuro-transmitters and their receptors.
Preparation of tissues for EM.
Advantages of Electron Microscopy in Neuroscience
The resolving power of the E.M. is much greater than that of the light microscope, which permits the visualization of sub-cellular structure. With the JEOL 1210, structure can be resolved at greater than 200,000X
This permits the visualization of both afferent and efferent elements of synapses and the precise definition of neuronal circuits. Circuits can be defined, for example, as axo-somatic, axo-dendritic, axo-axonic, myelinated, unmyelinated, etc.
It is difficult to see the forest because of the trees.
Sampling area is very limited.
Requires preliminary survey with light microscopy.
Each labeled CNS element requires a different electron dense, black and white marker (DAB, TMB, Colloidal gold, Viruses). NO COLORS, correspondingly the state-of-the-art is only 2 elements labeled simultaneously.
Normal ultrastructure is compromised by immunocytochemical or histochemical labeling, making correct identification of structure difficult.
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