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Electron Microscopic Image Analysis
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Electron Microscopic Image Analysis

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  • 1. The Laboratory for Electron Microscopic Image Analysis V. J. Massari, PhD
  • 2. Areas of Current Expertise
    • Ultrastructure of the CNS and Peripheral Nerves
    • Immunocytochemistry
    • Histochemistry
    • Definition of Neural Circuits using:
    • Anterograde Transport of Neural Tracers (Biotin)
    • Retrograde Transport of Neural Tracers (CTB)
    • Trans-synaptic Transport (Pseudorabies Virus)
    • Immunocytochemical labeling of neuro-transmitters and their receptors.
    • Preparation of tissues for EM.
  • 3. Advantages of Electron Microscopy in Neuroscience
    • Resolution:
    • The resolving power of the E.M. is much greater than that of the light microscope, which permits the visualization of sub-cellular structure. With the JEOL 1210, structure can be resolved at greater than 200,000X
    • This permits the visualization of both afferent and efferent elements of synapses and the precise definition of neuronal circuits. Circuits can be defined, for example, as axo-somatic, axo-dendritic, axo-axonic, myelinated, unmyelinated, etc.
  • 4. Disadvantages of Electron Microscopy
    • It is difficult to see the forest because of the trees.
    • Sampling area is very limited.
    • Requires preliminary survey with light microscopy.
    • Each labeled CNS element requires a different electron dense, black and white marker (DAB, TMB, Colloidal gold, Viruses). NO COLORS, correspondingly the state-of-the-art is only 2 elements labeled simultaneously.
    • Normal ultrastructure is compromised by immunocytochemical or histochemical labeling, making correct identification of structure difficult.
  • 5. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.

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