Biacore Molecular Interactions

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Biacore Molecular Interactions

  1. 1. Biacore Molecular Interactions Shared Resource (BMISR) Aykut Uren, MD, Director [email_address]
  2. 2. BMISR <ul><li>Have you ever wondered if two molecules directly interact with each other ? </li></ul><ul><li>If they do, how strong is that interaction? </li></ul><ul><li>Were you frustrated because you didn’t have the necessary technology or expertise to make it happen? </li></ul>
  3. 3. What can you use? <ul><li>Proteins </li></ul><ul><li>DNA </li></ul><ul><li>RNA </li></ul><ul><li>Small molecules </li></ul>
  4. 4. What can you do? Detect Binding Yes/No Identify Specificity Binding Partners Characterize Affinity Kinetics Concentration Thermodynamics
  5. 5. Biacore T-100 Integrated Microfluidics Gold Sensor Chip SPR Detection System
  6. 6. Biacore T-100 <ul><li>Real Time </li></ul><ul><li>See binding as it happens </li></ul><ul><ul><li>Unique binding information (on- and off-rates) for any size of biomol ecule </li></ul></ul><ul><ul><li>Study weak or fast interactions </li></ul></ul><ul><li>Label Free </li></ul><ul><ul><li>No labels required </li></ul></ul><ul><ul><li>Measure binding properties of unmodified substances </li></ul></ul><ul><ul><li>Reduce time and workload </li></ul></ul><ul><li>Contract Free </li></ul><ul><ul><li>Non-invasive </li></ul></ul><ul><ul><li>Measure opaque or colored samples </li></ul></ul>
  7. 7. Sample Applications (Chang) <ul><li>Protein DNA interactions : </li></ul><ul><li>DNA repair enzymes binding to DNA </li></ul><ul><li>Transcription factors binding to DNA </li></ul><ul><li>Small Molecule Protein Interactions: </li></ul><ul><li>Small molecule inhibitors of enzymatic activities </li></ul><ul><li>Small molecule inhibitors of protein- protein interactions </li></ul>Protein peptide interactions Protein RNA interactions
  8. 8. Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01    In your publications, remember to mention support provided from the GHUCCTS.

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