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  1. 1. A Study of Crude Extracts from Leaves of Piper samentosum Roxb. for Inhibiting Growth of Culex spp. Surakiat Leelasithorn Budsarin Muikeao Mahidol Wittayanusorn School,THAILAND
  2. 2. Method
  3. 3. Solvent Extraction Preparing the leaves of Piper Sarmentosum Roxb. Use Hexane as Solvent Use Ethyl Alcohol as Solvent Filtering the wastes Evaporating the solvents Weighing and recording the result Soaking for 5 days
  4. 4. Calculated LC 50 24 hr Preparing the larva of Culex spp. Testing for the death at different conc. Recording the results Analyzing the data Estimating LC 50 value
  5. 5. Result
  6. 6. Solvent Extraction <ul><li>Table 1: The weights of the crude extracts from each solvent for 5 times </li></ul><ul><li>* each time use 100 grams of the reagent </li></ul>
  7. 7. Solvent Extraction <ul><li>Table 2 : The mean weight of the extracts from each solvent </li></ul><ul><li>The data is computed by Statistical Package for the Social Sciences (SPSS). </li></ul>
  8. 8. Solvent Extraction
  9. 9. Calculated LC 50 24 hr Table3 : Percentage of dead larva in the crude extract by ethyl alcohol
  10. 10. Calculated LC 50 24 hr Data analysis by SPSS shows that The regression is R 2 =0.972 So we can estimate LC 50 value from the equation is 1 g/l
  11. 11. Calculated LC 50 24 hr Table4 : Percentage of dead larvas in the crude extract by hexane
  12. 12. Calculated LC 50 24 hr The regression is R 2 =0.988 So we can estimate LC 50 value from the equation is 0.67 g/l Data analysis by SPSS show that
  13. 13. Conclusion & Discussion <ul><li>From Table 1 and 2 : From 100 grams of reagent, the average weight of crude extract from Ethyl alcohol and Hexane is 2.04 and 1.51 grams , the mean difference is 0.53 grams, the difference is statistical significance with 95 % confidence interval is 0.27158 to 0.78842 gram . </li></ul><ul><li>From Table 3 and 4 : The calculation of LC 50 at 24 hr. for the crude extracts by Ethyl alcohol is 1 g/l and by Hexane is 0.67 g/l. </li></ul><ul><li>Ethyl alcohol as solvent gives more crude extract than hexane but the LC 50 at 24 hr. of Ethyl alcohol is more concentrated than Hexane. </li></ul>

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