Your SlideShare is downloading. ×
D N A  Tiling And The  A F M
Upcoming SlideShare
Loading in...5

Thanks for flagging this SlideShare!

Oops! An error has occurred.


Saving this for later?

Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime - even offline.

Text the download link to your phone

Standard text messaging rates apply

D N A Tiling And The A F M


Published on

Published in: Technology, Design

  • Be the first to comment

  • Be the first to like this

No Downloads
Total Views
On Slideshare
From Embeds
Number of Embeds
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

No notes for slide


  • 1. DNA Tiling and the AFM
  • 2. DNA versus DNA Tiling
    • DNA is a nucleic acid formed by a double helix
    • DNA is also made of a negatively charged phosphate backbone, base pairs and bonds between those bases
    • DNA tiling is the analyzing and the engineering of DNA and its structures
    • DNA plasmids are circular double-stranded molecules
    Image Source Not Located
  • 3. DNA Structure Image location URL is
  • 4. The AFM
    • The Atomic Force Microscope, or AFM is a scanning probe microscope
    • On the cantilever is a tip that scans back and forth the surface of your sample
    • The microscope gives you enlarged images of the DNA on the sample surface
    Image Sources Not Found
  • 5. Reasons for AFM
    • Advantages:
      • Can create detailed, true 3-D images of surfaces unlike the electron microscope
      • Works in a normal environment with little to no sanitary measures being taken in the environment
    • Disadvantages:
      • Cannot scan a very large surface in comparison to the STM
      • Scans relatively slowly and the tip used to scan is easily breakable
    Image location URL is
  • 6. Silicon Wafers
    • Silicon is used as a surface for the DNA plasmids to sit on while being analyzed by the AFM
    • For plain imaging on the AFM, Silicon does not have to be used
    • Then why is silicon emphasized?
    Image from
  • 7. The Adhesive Monolayer
    • The silane layer on the silicon is negative
    • The phosphate backbone of the DNA plasmid is also negative
    • A molecule known as aminopropyltriethoxysilane, or APTES is used as an adhesive
  • 8. DNA Structures
    • In DNA there are unpaired bases that stick out of the structure
    • They can be used to attach other DNA sticky ends to them, creating a larger structure
    • Other tile-like but more complex DNA structures can be used in nanotechnology
    Image is from the “Nanotechnology and the Double Helix” article by Nadrian C. Seema n
  • 9. Nanotechnology
    • Nanotechnology is the building of structures in the range of nanometers
    • One example of the power of nanotechnology is the image to the right
    Image location URL is
  • 10. Why DNA Tiling?
    • One main goal is to use DNA as a programmable component with other nanostructures
    • DNA can be used to act as scaffolding to hold molecules in an organized crystalline pattern
    • DNA can be engineered to act as circuitry for computers