Microfluidic Technology for Assisted Reproduction Matthew B. Wheeler 1 and David J. Beebe 2 1 Department of Animal Sciences and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign; 2 Department of Biomedical Engineering University of Wisconsin-Madison Acknowledgements Eric Walters and Sherrie Clark @ UIUC Henry “Gripp” Zeringue @ UW Lorraine Leibfried-Rutledge @ Bomed Kathy Haubert @ Vitae LLC Funding Sources: CFAR, NIH, UIUC, USDA, UW
MIT technology review (2001) - “one of ten technologies that will change the world”
Point of care diagnostics
Discovery/screening (not just drug)
DNA manipulation and processing
Microfluidic Large-Scale Integration Thorsen et al. Science 298: 580-584. A microfluidic chip, fabricated from silicone elastomer, that contains 2056 integrated microvalves in an area of one square inch. The chip is analogous to an electronic comparator and is an example of microfluidic large-scale integration. The complex plumbing in the chip allows 512 chambers to be mixed pairwise, with individual addressing and recovery of the results. [Photo: S. Maerkl]
Rationale Handling Steps in Conventional IVF Handling Steps in µchannel IVF Load oocyte/embryo Remove embryo Change medium and/or add Sperm, etc.
Hardware Traditional Inefficient & labor intensive µFluidic Automate procedures and improve efficiencies
-integrity of food during transportation and storage
Animals health monitoring:
-presence of residues, antibiotics, pathogens, toxins;
-land, water and air pollution;
Opportunities for Nanotechnology in Agriculture and Food Systems Research
1). Develop agriculture and food systems related microfluidic devices that feature integrated operations, simple reliable components and low costs. 2). Integrate microfluidic devices flawlessly into a wireless-ID network. 3). Others ? ? ? ? Objectives of a National Research Program
1). The users are producers, processors and consumers. -applications must be simple, reliable and highly accurate 2). The “environment” is dirty. -samples need some processing (filtration, purification, etc.) 3). Agriculture and Food Systems are highly integrated. -applications need to be networked and results integrated 4). Single sensor applications are likely not inadequate. -fields, herds, flocks, elevators, trains, trucks, processors, manufacturers etc. are widespread How is Agriculture Different? (or how will USDA’s focus differ from DOD, NSF, NIH or NASA) Outcomes and impacts should address these issues!