Your SlideShare is downloading. ×
0
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Ab Formation 2001
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Ab Formation 2001

203

Published on

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
203
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
4
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. Ab formation
  • 2. Hallmarks of the Immune Response <ul><li>Self/Non-self Discrimination </li></ul><ul><li>Memory </li></ul><ul><li>Specificity </li></ul>
  • 3. Fate of the Immunogen <ul><li>Clearance after 1 o exposure </li></ul><ul><ul><li>Equilibrium phase </li></ul></ul><ul><ul><li>Catabolic decay phase </li></ul></ul><ul><ul><li>Immune elimination phase </li></ul></ul><ul><li>Clearance after 2 o exposure </li></ul><ul><ul><li>More rapid onset of immune elimination phase </li></ul></ul>Immune Elimination Phase Days after Injection 2 4 6 8 12 10 Amount of Circulating Ag (%) 25 75 100 50 Equilibrium Phase Catabolic Decay Phase
  • 4. Kinetics of the Ab Response T-dependent Ag; 1 o Response <ul><li>Lag phase </li></ul><ul><li>Log phase </li></ul><ul><li>Plateau phase </li></ul><ul><li>Decline phase </li></ul>Ag D a y s A f t e r I m m u n i z a t i o n A b T i t e r LAG LOG DECLINE PLATEAU
  • 5. Kinetics of the Ab Response T-dependent Ag; 2 o Response * Specificity <ul><li>Lag phase </li></ul><ul><li>Log phase </li></ul><ul><li>Plateau phase </li></ul><ul><li>Decline phase </li></ul>1 o Ag 2 o Ag D a y s A f t e r I m m u n i z a t i o n A b T i t e r
  • 6. Qualitative Ab Changes during 1 o and 2 o Responses <ul><li>Class variation </li></ul><ul><ul><li>1 o - IgM </li></ul></ul><ul><ul><li>2 o - IgG, IgA or IgE </li></ul></ul>1 o Ag 2 o Ag Total Ab IgM Ab IgG Ab D a y s A f t e r I m m u n i z a t i o n A b T i t e r
  • 7. Qualitative Ab Changes during 1 o and 2 o Responses <ul><li>Class variation </li></ul><ul><li>Affinity </li></ul><ul><ul><li>Affinity Maturation </li></ul></ul>1 o Ag D a y s A f t e r I m m u n i z a t i o n A f f I n I t y 2 o Ag IgG Ab IgM Ab High Dose Low Dose
  • 8. Qualitative Ab Changes during 1 o and 2 o Responses <ul><li>Class variation </li></ul><ul><li>Affinity </li></ul><ul><ul><li>Clonal selection </li></ul></ul><ul><ul><li>Somatic mutation </li></ul></ul>10 10 10 8 10 6 10 5 10 9 10 7 High Ag Concentration 10 10 10 8 10 6 10 5 10 9 10 7 Low Ag Concentration Moderate Affinity Ab High Affinity Ab
  • 9. Qualitative Ab Changes during 1 o and 2 o Responses <ul><li>Class variation </li></ul><ul><li>Affinity </li></ul><ul><li>Avidity </li></ul><ul><li>Cross reactivity </li></ul>
  • 10. Cellular Events in 1 o Response to T-dependent Ags <ul><li>Lag </li></ul><ul><ul><li>Clonal selection </li></ul></ul><ul><li>Log </li></ul><ul><ul><li>IgM </li></ul></ul><ul><ul><li>Class switching </li></ul></ul><ul><li>Stationary </li></ul><ul><li>Decline </li></ul><ul><li>Memory Cell Pool </li></ul>IgM Memory Cells IgG 1 o Ag
  • 11. Cellular Events in 2 o Response to T-dependent Ags <ul><li>Lag phase </li></ul><ul><ul><li>Virgin cells </li></ul></ul><ul><ul><li>Memory cells </li></ul></ul><ul><li>Log phase </li></ul><ul><ul><li>Pool size </li></ul></ul><ul><ul><li>IgG, IgA or IgE </li></ul></ul><ul><li>Stationary </li></ul><ul><li>Decline </li></ul><ul><ul><li>Sustained production </li></ul></ul>IgM Memory Cells IgG IgG Memory Cells Memory Pool Virgin B cell
  • 12. Memory T cells <ul><li>T Cells </li></ul><ul><ul><li>Virgin cells </li></ul></ul><ul><ul><li>Memory cells </li></ul></ul><ul><li>Th cells </li></ul><ul><ul><li>Cytokines </li></ul></ul><ul><li>Long Term Memory </li></ul>Th Memory Cells Th Th Memory Cells Memory Pool Virgin cell
  • 13. Kinetics of Ab Response to T-independent Ags <ul><li>4 Phases </li></ul><ul><li>IgM antibody </li></ul><ul><li>No secondary response </li></ul>1 o Ag 2 o Ag D a y s A f t e r I m m u n i z a t i o n A b T i t e r IgM Ab
  • 14. Class Switching <ul><li>DNA rearrangement </li></ul><ul><ul><li>Antigen dependent </li></ul></ul><ul><ul><li>Switch site </li></ul></ul><ul><ul><li>Same VDJ </li></ul></ul><ul><ul><li>T H cytokines </li></ul></ul>  L    3   2  1  4  1  2 P L V D J S S S S S S S S P L S   1  2 S S  Primary Transcript IgM or IgD Primary Transcript IgE RNA DNA DNA DNA Rearrangement L V D J V D J V D J
  • 15. Membrane vs Secreted Ig <ul><li>Differential pre-mRNA processing </li></ul><ul><ul><li>Membrane exons </li></ul></ul><ul><ul><li>Alternate polyA sites </li></ul></ul><ul><ul><li>Same VDJ region used </li></ul></ul>mRNA Primary Transcript H3 H4 M M H3 H4 M M AAAA Membrane Bound H3 H4 AAAA Secreted H3 H4 AAAA H3 H4 M M AAAA DNA Polyadenylation Sites

×