Control of Anthracnose of banana ( Kluai Khai ) by the biofumigant fungus  Muscodor albus  strain MFC2 Kusuma  Potchana Wo...
Purposes <ul><li>To test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana.  </li></ul><ul><li>To find ...
Laboratory <ul><li>Biotechnological laboratoy of Mahidol University </li></ul><ul><li>Biological laboratory of Mahidol Wit...
Method
<ul><li>Equipment and Substances   </li></ul><ul><ul><ul><li>Colletotrichum capsici  </li></ul></ul></ul><ul><ul><ul><li>C...
<ul><li>Part 1   Grow MFC2 on PDA </li></ul>Do hyphal tip with MFC2 Grow MFC2 for 10 -14 days   Method
<ul><ul><ul><li>Make nutrients for growing MFC2   </li></ul></ul></ul>Nutrient 1 Nutrient   2 Brown rice  15  grams   mill...
<ul><li>Part  2  Grow Pathogens </li></ul>Method
<ul><li>Part  3  Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA </li></ul>10 day control...
<ul><li>Part  4  Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2 </li></ul>7...
Results
Results Table  1  Test on PDA inhibit inhibit Pathogens after  5  day 7 5.3 Control 1.83 3.67 Average 2.7 3.6 3 1.6 3.7 2 ...
<ul><li>Table   2   Test on nutrient 1 </li></ul>Results Inhibit Inhibit Pathogen after 5 days 4.4 4.4 Control 2.17 0.6 3....
<ul><li>Table 3  Test on nutrient 3   </li></ul>Results inhibit Inhibit Pathogen after 5 days 4.4 4.4 Control 2.17 1.7 3.0...
<ul><li>Muscodor albus  Strain   MFC2  growed in two different nutrients in order to control two anthracnose pathogens of ...
Conclusions and Analysis
การวิเคราะห์ค่าความแตกต่าง Conclusions and Analysis <ul><li>The nutrient ; water, bran, brown rice and millet, mixed with ...
<ul><li>Test with banana, let farmer use and assess. </li></ul><ul><li>Find the proper temperature for growing MFC2. </li>...
<ul><li>Ezra, D., W.M. Hess and G.A. Strobel. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-produ...
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Control Of Anthracnose Of Banana (Kluai Khai) By The Biofumigant Fungus Muscodor Albus Strain Mfc2

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Control Of Anthracnose Of Banana (Kluai Khai) By The Biofumigant Fungus Muscodor Albus Strain Mfc2

  1. 1. Control of Anthracnose of banana ( Kluai Khai ) by the biofumigant fungus Muscodor albus strain MFC2 Kusuma Potchana Worraya Prompangta Chomporn Raksakiettisak Adviser Miss Aree Sakyim
  2. 2. Purposes <ul><li>To test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana. </li></ul><ul><li>To find the proper nutrient for MFC2 </li></ul>Research Boundary <ul><li>Testing MFC2 with two pathogen ; Colletotrichum capsici and Colletotrichum gloeosporioides </li></ul>ระยะเวลาที่ทำการศึกษา <ul><li>Twelve months (November 2006 - November 2007) </li></ul>Term of Research
  3. 3. Laboratory <ul><li>Biotechnological laboratoy of Mahidol University </li></ul><ul><li>Biological laboratory of Mahidol Wittayanusorn School </li></ul>
  4. 4. Method
  5. 5. <ul><li>Equipment and Substances </li></ul><ul><ul><ul><li>Colletotrichum capsici </li></ul></ul></ul><ul><ul><ul><li>Colletotrichum gloeosporioides </li></ul></ul></ul><ul><ul><ul><li>Muscodor albus strain MFC2 </li></ul></ul></ul><ul><ul><ul><li>Autoclave </li></ul></ul></ul><ul><ul><ul><li>Larminar flow </li></ul></ul></ul><ul><ul><ul><li>Pasteur pipettes </li></ul></ul></ul>วิธีดำเนินการทดลอง Method
  6. 6. <ul><li>Part 1 Grow MFC2 on PDA </li></ul>Do hyphal tip with MFC2 Grow MFC2 for 10 -14 days Method
  7. 7. <ul><ul><ul><li>Make nutrients for growing MFC2 </li></ul></ul></ul>Nutrient 1 Nutrient 2 Brown rice 15 grams millet 35 grams Bran 5 grams water 5 cm 2 Method millet 50 grams Bran 5 grams water 5 cm 2
  8. 8. <ul><li>Part 2 Grow Pathogens </li></ul>Method
  9. 9. <ul><li>Part 3 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on PDA </li></ul>10 day control 7 day 5 day Method
  10. 10. <ul><li>Part 4 Test the efficiency of MFC2 to inhibit the anthracnose pathogen in banana on nutrient 1 and 2 </li></ul>7 day 7 day Control Method
  11. 11. Results
  12. 12. Results Table 1 Test on PDA inhibit inhibit Pathogens after 5 day 7 5.3 Control 1.83 3.67 Average 2.7 3.6 3 1.6 3.7 2 1.2 3.7 1 C.capsici C.gloeosporioides Diameter (cm) Plate
  13. 13. <ul><li>Table 2 Test on nutrient 1 </li></ul>Results Inhibit Inhibit Pathogen after 5 days 4.4 4.4 Control 2.17 0.6 3.03 2.15 Average 2.5 1 4.2 1.25 3 3 0.4 1 2.8 2 1 0.4 3.9 2.4 1 No PDA PDA No PDA PDA C.capsici C.gloeosporioides Diameter (cm) Plate
  14. 14. <ul><li>Table 3 Test on nutrient 3 </li></ul>Results inhibit Inhibit Pathogen after 5 days 4.4 4.4 Control 2.17 1.7 3.06 2.03 Average 3.4 1.5 3 1 3 2.7 3.2 1.9 2 2 0.4 0.4 4.3 3.1 1 No PDA PDA No PDA PDA C.capsici C.gloeospoiroides Diameter (cm) Plate
  15. 15. <ul><li>Muscodor albus Strain MFC2 growed in two different nutrients in order to control two anthracnose pathogens of banana ; Colletotrichum capsici and Colletotrichum gloeosporioides can inhibit the two pathogens but does not make any difference. Considered with the quantity of the nutrients, MFC2 growed on nutrient mixed with MFC2 has more efficiency to control the pathogens than another which is growed without PDA. </li></ul>Results
  16. 16. Conclusions and Analysis
  17. 17. การวิเคราะห์ค่าความแตกต่าง Conclusions and Analysis <ul><li>The nutrient ; water, bran, brown rice and millet, mixed with PDA can grow the MFC2 well and costs low. So this is another way to grow MFC2 to inhibit the two anthracnose pathogens of banana; Colletotrichum capsici and Colletotrichum gloeosporioide s. </li></ul>
  18. 18. <ul><li>Test with banana, let farmer use and assess. </li></ul><ul><li>Find the proper temperature for growing MFC2. </li></ul>Advice การวิเคราะห์ค่าความแตกต่าง
  19. 19. <ul><li>Ezra, D., W.M. Hess and G.A. Strobel. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology . 150: 4023–4031. </li></ul><ul><li>Freeman, S., Y. Nizami, S. Dotan, S. Even and T. Sando. 1997. Control of Collectotrichum acutatum in strawberry under laboratory, greenhouse, and field conditions. Plant Disease July 1997: 749-752. </li></ul><ul><li>Kuo, K.C. 2000. Sensitivity of Mango Anthracnose Pathogen, Colletotrichumgloeosporioides , to the Fungicide Prochloraz. Proc. Natl. Sci. Counc. ROC(B) vol. 25 : 174-179. </li></ul><ul><li>Mercier, J. and J.I. Jiménez. 2003. Control of fungal decay of apples and peaches by the biofumigant fungus Muscodor albus. Postharvest Biology and Technology. 31: 1–8. </li></ul>Reference <ul><li>Strobel, G.A., E. Dirkse, J. Sears and C. Markworth. 2001. Volatile antimicrobials from Muscodor albus , a novel endophytic fungus. Microbiology . 147: 2943–2950. </li></ul><ul><li>Sutton, B.C. 1980. The Coelomycetes, Fungi Imperfecti with Pycnidia, Acervuli and Stromata . Kew Surrey, England. </li></ul>

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