2.15.2010 – ETC and ATPase<br /><ul><li>ETC Complexes
Ubiquinone is also co-enzyme Q which pumps two H+ across membrane after 2 e-
Upcoming SlideShare
Loading in …5
×

2.15.2010

281 views

Published on

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
281
On SlideShare
0
From Embeds
0
Number of Embeds
2
Actions
Shares
0
Downloads
1
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

2.15.2010

  1. 1. 2.15.2010 – ETC and ATPase<br /><ul><li>ETC Complexes
  2. 2. Ubiquinone is also co-enzyme Q which pumps two H+ across membrane after 2 e-
  3. 3. Cytrochrome c transfers e-s to the complex III
  4. 4. Complex II is embedded in complex I
  5. 5. NADH tranfers e-s to heme groups in complex I
  6. 6. See slide 31
  7. 7. ATPase is made of two complexes: F0 and F1 pumps H+ through, F1 generates ATP. Every 3H+ is one ATP generated.
  8. 8. Must pump about 104 H+ through
  9. 9. Coenzyme Q and Cytochrome C - slide 30
  10. 10. Move within the membrane
  11. 11. C and Q have an iron heme group that holds the e-s and carry them through the ETC
  12. 12. O2 as the Final e- Acceptor
  13. 13. There are two heme groups: heme A and heme A3
  14. 14. Respiratory Complexes – slide 33 (KNOW Table)
  15. 15. NADH-COENZYME Q OXIDOREDUCTASE
  16. 16. Succinate dehydrogenase
  17. 17. Useful with FADH2. It will donate its e- to succinate dehydrogenase
  18. 18. FADH2 is less useful in making H+ motive force because FADH skips first complex. FADH2 only pumps out 6 instead of 10 H+
  19. 19. Useful to see who the e- were accepted from and who they were passed to.
  20. 20. NOTE: e- outside the mitochondria do not move into the membrane of the mitochindria, so the e-s in the mitochondria are the only e- in there when it comes to the ETC
  21. 21. ATP Synthesis - 34
  22. 22. See table
  23. 23. FO is the protein channel that pumps H+ across membrane
  24. 24. C proteins rotate and help turn gamma subunit of F1, Gamma transmits energy as rotation occurs
  25. 25. ATP is made in the matrix by F1
  26. 26. FO rotates c units, turning gamma subunit
  27. 27. Core, A protein
  28. 28. B, connects to f1
  29. 29. C rotational complex
  30. 30. Alpha and Beta subunit complex
  31. 31. Alpha
  32. 32. Beta units are the enzymatic subunits that bind to ADP and make ATP
  33. 33. Proton motive force is used to drive ATP synthesis
  34. 34. Chemiosmotic coupling drives substrate phosphorylation
  35. 35. ATP synthase is reversible. So instead of generating, it hydrolyzes ATP
  36. 36. Binding-Change Mechanism – 36
  37. 37. Beta subunits bind to ADP and phosphate and change comformational
  38. 38. Open, Beta 1, binds inorganic phosphate which causes rotation of gamma subunit that moves complex ~ 120 degrees.
  39. 39. Loose – ADP and phosphate, and ATP bound by next subunit is released. To be dropped in matrix to be used by cell.
  40. 40. Tight
  41. 41. Each 120 rotation releases an ATP
  42. 42. ATP synthesis is Spontaneous - 37
  43. 43. The proton gradient is -4.6kcal/mol, but the synthesis is endergonic
  44. 44. 104 H must be pumped to make 34 ATP in oxidative phosphorylation
  45. 45. Yield – 38
  46. 46. Glycerol Phosphate Shuttle – 38
  47. 47. Pyruvate > Krebs cycle
  48. 48. G3PDH – glycerol-3-phosphate accepts e-s and then goes into mitochondria through the porins in the membrane, which will then transfers e-s to FADH2 and into the ETC.
  49. 49. H+ are in inter membrane space, in the matrix the pH is more basic and a larger negative charge inside the membrane which will help power F1 to make ATP
  50. 50. Theoretical Yield - 41
  51. 51. GTP is theoretically the same as ATP
  52. 52. 36 ATP and 2 GTP
  53. 53. Energetics of the Process
  54. 54. To make 38 ATP – 55% efficiency
  55. 55. WHY?
  56. 56. Many intermediates in glycolysis and citric acid cycle use ATP, so only about 26 ATP are available afterwards
  57. 57. They are in the matrix, so need to be moved out
  58. 58. Voltage difference across membrane. ATP is slightly negative than ADP, so inside is negative and outside is positive, so the ATP wants to go to the outside. Through an ATP/ADP antiporter, so ADP is provided for ATP synthase
  59. 59. Phosphate transporter takes advantage of pH gradient. Inner is more basic than intermembrane space.
  60. 60. Fats as Energy Source – 49
  61. 61. Fatty acids can be broken to acetyl CoA
  62. 62. Mitochondrial matrix is where it occurs (slide 50)

×