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1. Enhancement of Plant Growth,
Synthesis of Sugar Compound,
and Stevioside from Stevia
(Stevia rebaudiana Bertoni)
Using White, Red, And Blue LED
Light
Muhammad Rizky Hanan Putra
Dr. Rizkita Rachmi Esyanti
Dr. Robert Manurung
School of Life Sciences andTechnology
InstitutTeknologi Bandung
2014
rizkyhananputra@gmail.com

2. Need of Research
• Limiting Factor  Flowering Process  Decreasing Accumulation of Stevioside in
Leaves
• Flowering Process could be controlled with modification of photoperiodism
• Red Light  Inhibit Flowering in Xanthium (Taiz & Zeiger, 2002)
Blue Light  Affect Flowering in Arabidopsis (Taiz & Zeiger, 2002)
• High sweet intensity 150- 400 X sucrose
• Low calorie
• Noncarcinogenic
• Highest component  Stevioside (5 – 10 % dry leaf)
(Isdianti, 2007 ;Kinghorn, 2002:Savita dkk, 2004)
Stevia (Stevia rebaudiana Bertoni)
Contained sweetener compound in the leaves
 steviol glycosides
( diterpenoid glycosides) (synthesized from methylerythritol- 4- phosphate (MEP) pathway)

3. Aim of Study
• To evaluate whether the parameters of growth and
development of the stevia plant, such as plant height and
weight, total sugar content, and accumulation of steviol
glycosides compounds, especially stevioside, could be
influenced by the three photoreceptors.

4. Methodology

5. Result
• Plant Growth :
Height
Light
Type
Specific Growth
Rate (mm/ day)
White 1,54
Red 1,1
Blue 1,85
Control 1,89
Figure 1. Growth Kinetic
Tabel 1. Specific Growth Rate
0
4.85
0
3.61
0
2.86
0
4.95
0
1
2
3
4
5
6
0 14
AverageHeightIncrement(cm)
Day
Control
White
Red
Blue

6. • Plant Growth
DryWeight
Light
Type
Specific Growth Rate
(mg/ day)
DoublingTime (day)
White 3,5 19,87
Red 3 22,57
Blue 3,8 17,85
Control 3,4 20,3
Figure 2. Growth Kinetics
Tabel 2. SpecificGrowth Rate
dan DoublingTime
0
110
0
113
0
96.7
0
130
0
20
40
60
80
100
120
140
0 14
AverageWeightIncrement(mg)
day
Control
White
Red
Blue

7. Flowering Comparison
Figure 3. Flowering Process in Plants (14th Day)
Control White Blue Red
Flowering Process Flowering Process
X

8. Sugar Compound and Stevioside
Figure 4. Sugar Brix Content Figure 5. Stevioside Content
0.023
0.046
0.141
0.031
0.000
0.020
0.040
0.060
0.080
0.100
0.120
0.140
0.160
Control White Red Blue
AverageStevioside
Content(%)
Treatment
20
20.5
21
20.5
19
19.5
20
20.5
21
21.5
Control White Red Blue
AverageBrixDegree(%)
Treatment
( 5 % )* ( 90,5 % )*
*Against Control

9. Conclusion
• Exposure to red, blue, and white light affects some growth
parameters, namely height and dry weight increment of
plants, plant development (Flowering), and plant metabolism
(Sugar and Stevioside content).
• Blue light exposure causes plants to exhibit the highest
height increment rate and dry weight gain.
• Plants with red light exposure exhibit delayed flowering and
increased steviosida and total sugar content.

10. References
Brandle, J. E . &Telmer, P. G . 2007. Steviol glycoside biosynthesis. Phytochemistry 68 :1855- 1863
Ceunen, S. & Geuns, J. M. C. 2013. Glucose, sucrose, and steviol glycoside accumulation in Stevia rebaudiana grown under different
photoperiods. Biologia Plantarum 57 (2) : 390- 394
Ceunen, S. & Geuns, J. M. C. 2013. Steviol glycosides : chemistry diversity, metabolism, and function. Journal of Natural Product 76
(6) : 1201 – 1228
Ceunen, S., Werbrouck, S. & Geuns, J. M. C. 2012. Stimulation of steviol glicoside accumulation in Stevia rebaudiana by red LED light.
Journal of Plant Physiology 169 : 749 – 752
Josse, E. & Halliday,K. J. 2007. Photoreceptor interactions with other signals. Singapura. Blackwell Publishing.
Kinghorn, A. D. 2002. SteviaThe Genus Stevia . London. Taylor & Francis.
Kondo, S., Tomiyana, H., Rodyoung, A., Okawa, K., Ohara, H., Sugaya, S., Terahara, N., dan Hirai, N. 2014. Abscisic acid metabolism
and anthocyanin synthesis in grape skin are affected by light emitting diode (LED) irradiation at night. Journal of Plants Physiology
171, 823-829.
Loveys, B. R. &Wareing, P. F. 1971.The red controlled production of gibberellin in etiolated wheat leaves. Planta, 98 : 109- 116
Savita,S,M., Sheela,K., Sunanda,S., Shankar, A,G. & Ramakrishna, P. 2004. Stevia rebaudiana a functional component for food
industry. J.Hum. Ecol, 15 (4) : 261-264
Taiz, L. & Zeiger, E. Plant Physiology 3rd Edition. Sunderland. Sinauer
Whitelam, G. & Halliday, K. J. 2007. Light and plant development. London. Blackwell Publishing Ltd.

11. ThankYou
Terima Kasih