What’s a biofilm?
A biofilm is a structured community of microorganisms encapsulated within a
self-developed polymeric matrix and adherent to a living or inert surface.
Biofilms are also often characterized by surface attachment, structural
heterogeneity, genetic diversity, complex community interactions, and an
extracellular matrix of polymeric substances.
Biofilms are ubiquitous.
Biofilms can be found on rocks and pebbles at the bottom of most
streams or rivers and often form on the surface of stagnant pools
Biofilms grow in hot, acidic pools in Yellowstone National Park
(USA) and on glaciers in Antarctica.
Biofilms in showers (Legionella).
Plaque formation on teeth.
Why a biofilm?
Protects the bacteria
Secretes virulence factors
Gram negative rod
Fluoresces on certain types of agar
Prevalent in Hospitals due to it resistance to disinfectants
Particular problem in the cystic fibrosis community
plank tonic attached to biotic or abiotic
Cells undergo a program of physiological changes involving
Production of copious quantities of extracellular
Macrophage migration inhibitory factor (MIF)
Enhance Pseudomonas aeruginosa growth both in vivo & in vitro
MIF enhances biofilm formation
Culture Pseudomonas aeruginosa
Micro Arrays looking at gene expression
Target MIF therapeutically?
Disrupt biofilm formation
Antibiotics time to work
New antimicrobial defence for CF patients