Bacterial biofilms

2,231 views

Published on

A simple presentation explaining the basics of bacterial biofilms

Published in: Health & Medicine, Technology
0 Comments
1 Like
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
2,231
On SlideShare
0
From Embeds
0
Number of Embeds
14
Actions
Shares
0
Downloads
137
Comments
0
Likes
1
Embeds 0
No embeds

No notes for slide

Bacterial biofilms

  1. 1. Bacterial Biofilms
  2. 2. What’s a biofilm? A biofilm is a structured community of microorganisms encapsulated within a self-developed polymeric matrix and adherent to a living or inert surface. Biofilms are also often characterized by surface attachment, structural heterogeneity, genetic diversity, complex community interactions, and an extracellular matrix of polymeric substances.
  3. 3. Examples Biofilms are ubiquitous. Biofilms can be found on rocks and pebbles at the bottom of most streams or rivers and often form on the surface of stagnant pools of water. Biofilms grow in hot, acidic pools in Yellowstone National Park (USA) and on glaciers in Antarctica. Biofilms in showers (Legionella). Plaque formation on teeth.
  4. 4. Why a biofilm? Advantages Protects the bacteria Antibiotics Phagocytised Metabolically inactive Secretes virulence factors
  5. 5. Pseudomonas aeruginosa Gram negative rod Opportunistic pathogen Fluoresces on certain types of agar Prevalent in Hospitals due to it resistance to disinfectants Particular problem in the cystic fibrosis community Antibiotic resistant
  6. 6. Biofilm formation Development initiates: plank tonic attached to biotic or abiotic surfaces. Cells undergo a program of physiological changes involving Quorum sensing. Production of copious quantities of extracellular polysaccharides.
  7. 7. Our Interest Macrophage migration inhibitory factor (MIF) Enhance Pseudomonas aeruginosa growth both in vivo & in vitro Hypothesis MIF enhances biofilm formation Culture Pseudomonas aeruginosa +/-MIF +/- Oxygen Biofilm formation Resazurin assay Micro Arrays looking at gene expression
  8. 8. Possibilities Target MIF therapeutically? Inhibitors Disrupt biofilm formation Antibiotics time to work New antimicrobial defence for CF patients

×