ProfessorYuka Itokawa , ProfessorYeunhwa GU  Prepared by: Japan Suzuka University of  Medical Science   30P1-am238 Registe...
Research Background <ul><li>In late years, death by cancer, metabolic syndrome, diabetes, heart disease, cardiovascular di...
Methods 2 <ul><li>   Experimental group </li></ul><ul><li>①  Control group </li></ul><ul><li>②  Herbs treated group </li><...
Methods 3 <ul><li>The SOD measurement </li></ul><ul><li>We collect blood and measure blood using a measurement kit from no...
Antioxidation assay <ul><li>                   NO 2 - TB </li></ul><ul><li>  XA           2 O 2 -・          </li></ul><ul>...
Methods 4 (Anti-cancer effect) <ul><li>Animal used for experiment: C3H mouse (♂) four weeks </li></ul><ul><li>It is the ri...
Methods 5 <ul><li>   E xperimental group </li></ul><ul><li>①  Control group </li></ul><ul><li>②  Herb treated group </li><...
Methods 6 <ul><li>Tumor stereometry </li></ul><ul><li>We measured it more for 30 days every 2nd cancer inoculation one wee...
SOD-like activity (after 15th) This histograph is the image of SOD ( 15days later ) . Each  histograph represents the mean...
SOD-like activity (after 30th) This histograph is the image of SOD ( 30days later ) .Each histograph represents the mean v...
leukocytes This change in number of leukocytes the blood took from the tail vein ICR mice. Each line gram represents the m...
This change in number of  l ymphocyte the blood took from the tail vein ICR mice. Each line gram represents the mean value...
Effect on anti-tumor by the sample medication. Each line represents the mean value ±S.E  Analyzed by Dunnett-test. *  *  *...
Weight of tumor. Each line represents the mean value ±S.E. Analyzed by Dunett-test (n=7) Gu Lab Tumor weight (non-irradiat...
Weight of tumor. Each line represents the mean value ±S.E.  It was analyzed by Dunett-test (n=7) Gu Lab Tumor weight (irra...
The free radical removal by SOD O2 O2  - An injury factor C H O DAN polymerase SH A replication of DNA error Chromosomal a...
Reinforcement of removal + biophylaxis ability of a cause tumor inhibition mechanism Free radical elimination / antioxidat...
Structure of tumor immunity by antigen-antibody reaction Natural killer cell Antigen fault acquired immunity Activation Na...
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The Immune System, Anticancer Mechanism , Enzyme

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  • アロエべラと酵素の単独及び併用 活性水素水における免疫効果 と抗腫瘍効果などのメカニズムに関する研究について 具研究室 永井、花井が報告致します。
  • はじめに研究背景です。近年、癌、心疾患、脳血管疾患などによる死亡が上位を占めるようになりました。このうち、悪性新生物による死亡数は年々増加傾向にあり、死因の 1 位を占めています。 そのため、難治性癌を克服するためにいろいろな対策が講じられています。末期医療や緩和医療によって癌で苦しまないようにする対策も充実してきました。 (☆) 癌そのものにならない予防法を推進することこそ癌克服の根本的な解決策であると考えられます。 (☆) そこで注目されるのが天然物を用いた代替医療です。代替医療は、放射線治療などによる副作用などが少なく、天然物質を組み合わせることで免疫を高め、癌の成長など遅らせることができるのではないかと考えられています。
  • The Immune System, Anticancer Mechanism , Enzyme

    1. 1. ProfessorYuka Itokawa , ProfessorYeunhwa GU Prepared by: Japan Suzuka University of Medical Science 30P1-am238 Register #
    2. 2. Research Background <ul><li>In late years, death by cancer, metabolic syndrome, diabetes, heart disease, cardiovascular disease, liver, and kidney have increased. </li></ul><ul><li>There is an overall increase of people dying by a malignant neoplasm </li></ul><ul><li>Overall imbalances of human conditions have increased and enzymes ingestions has decreased. </li></ul><ul><li>I Methods1 (immunological enhancement effects) Animal used for experiment: ICR mouse (♂) four week </li></ul><ul><li>An experimental group several:10 group (each eight groups) </li></ul><ul><li>Dosage: 0.01ml/g </li></ul><ul><li>Medication method: oral administration (every day): </li></ul><ul><li>It is distilled water of the same amount in control group </li></ul><ul><li>Administration level: 500mg/kg </li></ul><ul><li>Irradiation condition: 2Gy radiation bath an administration start two weeks later </li></ul><ul><li>(200KV, 9mA, 0,341Gy / min) </li></ul>
    3. 3. Methods 2 <ul><li>   Experimental group </li></ul><ul><li>① Control group </li></ul><ul><li>② Herbs treated group </li></ul><ul><li>③ Enzyme treated group </li></ul><ul><li>④ Herbs + enzyme treated group </li></ul><ul><li>⑤ 2Gy independent irradiation group </li></ul><ul><li>⑥ Herb+ irradiation group </li></ul><ul><li>⑦ Enzyme + irradiation group </li></ul><ul><li>⑧ Herb + enzyme + irradiation group </li></ul>
    4. 4. Methods 3 <ul><li>The SOD measurement </li></ul><ul><li>We collect blood and measure blood using a measurement kit from non-irradiation group mouse (after post radiation 15th, 30th) after having centrifuged it. </li></ul><ul><li>The peripheral blood cell measurement </li></ul><ul><li>We collect blood of a caudal vein of a mouse (on three hours post radiation administration on the irradiation day before, 12 hours, 24 hours, 3rd, 7th, 15th) </li></ul><ul><li>We do on 30th and measure white-cell count, lymphocyte counts in an automatic blood corpuscle measuring instrument. </li></ul>
    5. 5. Antioxidation assay <ul><li>                   NO 2 - TB </li></ul><ul><li>  XA          2 O 2 -・          </li></ul><ul><li>   </li></ul><ul><li>     </li></ul><ul><li>XOD   37℃ warm   SOD </li></ul><ul><li>                   </li></ul><ul><li>H 2 O 2 + O 2 </li></ul><ul><li>  </li></ul><ul><li>   2 O 2 -・ +2 H +        H 2 O 2 + O 2 </li></ul><ul><li>Pigment ( λ max=530nm) ) it is reduced to elements than xihormazan ‥ N-TB, and to make self-possession in a dehydrase case </li></ul>
    6. 6. Methods 4 (Anti-cancer effect) <ul><li>Animal used for experiment: C3H mouse (♂) four weeks </li></ul><ul><li>It is the right foot for a tumor (SCC -7) than starting administration two weeks later </li></ul><ul><li>Subcutaneous to a femoral region let take it. </li></ul><ul><li>Experimental group several:10 groups </li></ul><ul><li>Dosage: 0.01ml/g </li></ul><ul><li>Medication method; oral administration (every day): </li></ul><ul><li>It is distilled water of the same amount in control group </li></ul><ul><li>Administration level: 500mg/kg </li></ul><ul><li>Irradiation condition: right foot femoral region an after tumor intake 10th </li></ul><ul><li>We performed local irradiation to a chisel. </li></ul><ul><li>(200KV, 9mA, 0,341Gy/min) </li></ul><ul><li>We irradiate it every 6Gy 1st of *3 times 2Gy in total. </li></ul>
    7. 7. Methods 5 <ul><li>   E xperimental group </li></ul><ul><li>① Control group </li></ul><ul><li>② Herb treated group </li></ul><ul><li>③ Enzyme treated group </li></ul><ul><li>④ Herb + enzyme treated group </li></ul><ul><li>⑤ 2Gy independent irradiation group </li></ul><ul><li>⑥ Herb + irradiation group </li></ul><ul><li>⑦ Enzyme + irradiation group </li></ul><ul><li>⑧ Herb + enzyme + irradiation group </li></ul>
    8. 8. Methods 6 <ul><li>Tumor stereometry </li></ul><ul><li>We measured it more for 30 days every 2nd cancer inoculation one week later. </li></ul><ul><li>Tumor volume </li></ul><ul><li>Tumor weight </li></ul><ul><li>We resect a tumor than a cancer inoculation after 30th and weigh a tumor, We found tumor inhibition rate. </li></ul><ul><li>Tumor inhibition rate (%)= </li></ul><ul><li>Tumor weight (g) of control group CW </li></ul><ul><li>Tumor weight (g) of specimen group TW </li></ul>
    9. 9. SOD-like activity (after 15th) This histograph is the image of SOD ( 15days later ) . Each histograph represents the mean value ±SE. SOD-like activity (after 15th)
    10. 10. SOD-like activity (after 30th) This histograph is the image of SOD ( 30days later ) .Each histograph represents the mean value ±SE. Analyzed by Dunett-test SOD-like activity (after 30th) *
    11. 11. leukocytes This change in number of leukocytes the blood took from the tail vein ICR mice. Each line gram represents the mean value ±SE. Analyzed by Fisher-test. *p<0.05,**p<0.01 vs control. (↑ 2Gy whole body irradiation ) * ** ** ** * * * * * * Gu Lab
    12. 12. This change in number of l ymphocyte the blood took from the tail vein ICR mice. Each line gram represents the mean value ±SE. Analyzed by Fisher-test. *p<0.05,**p<0.01 vs control (↑ 2Gy whole body irradiation ) * * * * * * l ymphocyte Gu Lab
    13. 13. Effect on anti-tumor by the sample medication. Each line represents the mean value ±S.E Analyzed by Dunnett-test. * * * * * * * * * * * * * * * * * * * * * * * * * * Tumor volume (non-irradiation group) * * * * * * * * * * * * * * * * *
    14. 14. Weight of tumor. Each line represents the mean value ±S.E. Analyzed by Dunett-test (n=7) Gu Lab Tumor weight (non-irradiation group)
    15. 15. Weight of tumor. Each line represents the mean value ±S.E. It was analyzed by Dunett-test (n=7) Gu Lab Tumor weight (irradiation group)
    16. 16. The free radical removal by SOD O2 O2 - An injury factor C H O DAN polymerase SH A replication of DNA error Chromosomal anomalies Approval of autoimmune disease An auto-immunity antigen A - antibody complex Bacteria A virus XOD blood cells, a macrophageWhite Stimulation We remove to SOD Deactivation
    17. 17. Reinforcement of removal + biophylaxis ability of a cause tumor inhibition mechanism Free radical elimination / antioxidation action The conduction that detoxication / Radhi Cal elimination is enzymatic Anti-inflammatory action DNA disorder (mutation) DNA restoration A mutation cell Cytostasis / apotosis conduction A cancer cell
    18. 18. Structure of tumor immunity by antigen-antibody reaction Natural killer cell Antigen fault acquired immunity Activation Natural killer cell A cancer cell An antibody An antibody-producing cell A killer cell T-helper cells B cell Antigen presentation A macrophage Antigen presenting cells Antigen acquired immunity A carcinoma antigen
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