Aplikasi Klinis Stem Cell by Dr. Indah Yulianto

2,330 views
2,306 views

Published on

A comprehensive presentation on Clinical Application of Stem Cell

Published in: Health & Medicine
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
2,330
On SlideShare
0
From Embeds
0
Number of Embeds
3
Actions
Shares
0
Downloads
44
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide
  • Abbreviations: ICAM, intercellular adhesion molecule; JAMs, junctional adhesion molecules; MSCs, mesenchymalstromal cells; PECAM, platelet-endothelial cell-adhesion molecule; PGE, prostaglandin E2; VCAM, vascular cell-adhesion molecule; VLA, very late antigen
  • Aplikasi Klinis Stem Cell by Dr. Indah Yulianto

    1. 1. APLIKASI KLINIS SEL PUNCA
    2. 2. Differentiation Human Stem Cells
    3. 3. Trans-differentiation
    4. 4. Why Stem Cells ? By definition, a stem cell is characterized by its ability to self- renew and its ability to trans differentiate along multiple lineage pathways.
    5. 5. Self-renewal of stem cellsAsymmetric cell divisionSymmetric cell divisionMorrison & Kimble 2006
    6. 6. Self-renewal of stem cells Asymmetric cell division : to maintain the stem cell numberA. Intrinsic asymmetric cell divisionB. Extrinsic (Eviromental) asymetric cell devision Morrison & Kimble 2006
    7. 7. Intrinsic asymmetric cell division One stem cell divides into two daughter cells, which are different from each other. One of daughter cells is similar to the parental cell and The other daughter cell becomes progenitor cell, which will eventually differentiate into tissue committed resident cell.
    8. 8. Symmetric cell division Daughter cells
    9. 9. Stem cells NICHE The microenvironment in which stem cells are found. The microenvironment interacts with stem cells to regulate stem cell fate. Various niche factors act on stem cells to alter their gene expression, and induce their proliferation or differentiation.
    10. 10. Stem Cells NICHENiche factors include: 1. cell-cell interactions between stem cells, 2. interactions between stem cells and neighboring differentiated cells, 3. interactions between stem cells and extra- cellular matrix components, adhesion molecules,growth factors, cytokines and physiochemical nature of the microenvironment. 4. Niche has both anatomic and functional dimensions.
    11. 11. Regenerative medicinal applications should meet the following criteria: Can be found in abundant quantities (millions to billions of cells) Can be harvested by a minimally invasive procedure Can be differentiated along multiple cell lineage pathways in a regulatable and reproducible manner. Can be safely and effectively transplanted to either an autologous or allogeneic host
    12. 12. Overview of Healing response after injury
    13. 13. Goal of The Wound HealingReconstruction of : Original Architecture Function of the injured tissue. (Cotran et.al. 1999., Majno & Joris 2004)
    14. 14. Tissue Wound Healing Repair Regeneration (Majno & Joris 2004, Kumar et al. 2009)
    15. 15. Tissue Repair Replacement of injured tissue by different tissue, usually by fibrosis or scar (Kumar et al. 2009, Majno & Joris 2004)
    16. 16. Tissue Regeneration Replacement of injured tissue by the same resident cells, or by differentiation of progenitor/stem cells into tissue committed cells. (Kumar et al. 2009, Majno & Joris 2004)
    17. 17. PERANAN BERBAGAI KOMPONEN PADA “NICHE” JARINGAN KULIT (Copyright © 2012 VictorW.Wong et al.)
    18. 18. Stem Cells Transplantation Autologous Allogeneic : from individuals belonging to the same species but genetically dissimilar (and hence immunologiccally incmpatible) Syngeneic : between geneticcally identical individual (tween) Xenogeneic : From individuals of different species (hence of disparate cell type)
    19. 19. Adult (Somatic) Stem Cells Hematopoietic stem cells (HSC) Mesenchymal stem cells (MSC) Endothelial progenitor cells (EPC) Neural stem cells Hepatic stem cells etc. Adipocyte Stem Cells
    20. 20. Why Adipocyte Tissue ?
    21. 21. Bone marrow harvest (2)
    22. 22. Flow Chart Elucidating possible commitment,lineage progression and maturation ADMSCs from Aldeman D., Alexander ,R.W.,PPM : Oct 2011
    23. 23. Hasil lemak dari liposuction
    24. 24. pellet
    25. 25. Adipocyte after 24 hours Adipocyte after 24 hours ADSC pasage 1 : hari ke 3
    26. 26. A. ADSCs hari ke 7 dan B. ADSC hari ke 14A B by dr Indah Yulianto – dr. Yuyun Rindiastuti
    27. 27. Alopecia Universalis : 1 month after MChSc and Product (AAPE)
    28. 28. Alopecia Universalis :1 month after MCSc + Product MSCc By Indah Yulianto
    29. 29. After 2 Months
    30. 30. After :2 Months By Indah Yulianto
    31. 31. After 4 months
    32. 32. EPC : Primer Culture 80% 100% Courtesy by : Yuyun, Wiwied & Yenni
    33. 33. SECRETOME FROM HUVEC (HUMAN UMBILICALVEIN ENDOTHELIAL CELLS) from patient Alopecia Areata for more than 10 years.
    34. 34. Secretome from HUVEC
    35. 35. Homing and Trafficking MesenchymalStromal cells to sites tissue injury after infusion
    36. 36. Neuroischemic lesion of fifth metatarsal withapplication : EPC & amnion membrane (2 months) Courtesy : By Indah Yulianto, &Yuyun Rindiastuti
    37. 37. Female patient (64 years old) with Basaloma in her left cheek
    38. 38. Preparing Secretome from Wharton Jelly
    39. 39. BASALIOMA(Surgery + Secretome fetal Endothelial progenitor Cells / EPC)
    40. 40. Peeling the amniotic membrane from the placenta(Once again using Hypoxia methode) Figure 1: Processing of amniotic membrane.
    41. 41. Preparation of the amniotic membraneAmniotic membrane in its and the wound dressing interface before preserving fluid placement of a wound dressing.
    42. 42. No healing ulcer (1 month) post op-Squamous Cell Carcinoma Surgery in Head (Male, 84 yr) Placing AMNION
    43. 43. Wound Regeneration after 2 Surgeried : SCC and UNSECCESSFUL DermagraftHealing Process with renewal damage tissue
    44. 44. AMNIOTIC MEMBRANE DRESSING PROMOTES EPITHELISATION & GRAANULATION + INJ EPC 1.5 MONTH AFTER
    45. 45. CLI – post AmputationEPC + Amnion membrane
    46. 46. What kind of Stem cells that we Need ?
    47. 47. (3) Scar acne pasca skin needling + product & (4) 1 week after skin needling + MCSs +_ Product3 4
    48. 48. Acne Papulo Pustular + Scar Courtesy by dr Titik Moertolo
    49. 49. Acne Scar & Box scar 1 week post Dermaroller
    50. 50. 2 weeks after Skin Needling +Acne Scar before (Box Scar) MShCs Courtesy by dr Titik Moertolo
    51. 51. Infant 2 weeks, with fistula supra ani
    52. 52. Tissue regeneration after 1 week using MshCs Product - Cream BY Indah Yulianto
    53. 53. One week afterFistula – Before Therapy 1 Year after – no Scar
    54. 54. Leprosy after 30 years + 3 3 weeks after MSCs months burning ulcer + Infection Product Injections + Cream BY Indah Yulianto
    55. 55. Male 23 yr, Combutio more than 5 months : Injections and Topical : HUVECs (Spheroid and Hypoxia)25-Jan-13 68
    56. 56. Luka bakar + infeksi 5 bulan intra kutan produk MShCs y.l. regenerasi Hari ke 15 : penyembuhan 75% tanpaPengolesan Krem + Produk MShCs Cicatriks ataupun Retraksi persendian
    57. 57. Starting to heal and without scar after 2 months25-Jan-13 70
    58. 58. ACNE CONGLOBATA and Scar : USING ‘HUVEC’
    59. 59. AUTISM AND PARAPLEGIA (FEMALECHILD – 4 YEARS : USING HUVEC : 2 TIMES)
    60. 60. Multiple sclerosis , Asthma Bronchiale, Parkinson and Ventricle heart disease
    61. 61. Terimakasih atas perhatiannya25-Jan-13 74

    ×