LAB REVIEW<br />PARASITES<br />&<br />BLOOD CELLS<br />
Band Neutrophil<br />
Red Blood Cells<br />
Lymphocyte<br />
Eosinophil<br />
Platelets<br />
Basophil (in horses it is blue)<br />
Monocyte<br />
Segmented Neutrophil<br />
Tapeworm    <br />
Hookworm<br />
Heartworm   filaria<br />
Roundworm<br />
Coccidia<br />
Whipworm<br />
Mites<br />
Demodex<br />
Giardia<br />
FLEA<br />
Mite<br />
Babesia Canis<br />
Sucking Lice…………..Biting Lice<br />
Ehrlichia<br />
American Dog Tick….……..Deer Tick<br />
Lone Star Tick………Rocky Mtn Wood Tick<br />
Dirofilaria immitum….dipeteloma reconditum<br />
Hemobartonella???<br />
Avian Blood<br />
Warble or Cuterebra (fly larvae)<br />
Anaplasma Marginale<br />
Lung Worm<br />
paragonimus kellicotti<br />
Lung Fluke<br />
Eucoleus boehmi<br />
Urine<br />Physical Exam:<br />Smell<br />Color<br />Turbidity<br />Foam<br />
Urine<br />Chemical Exam:<br />pH<br />Bilirubin<br />Protein<br />Occult Blood<br />Glucose<br />Ketones<br />Specific gr...
Heartworm Test<br />Buffy Coat method (hematocrit test)<br />Modified Knott’s technique<br />Filter membrane technique/Di-...
Steps For <br />Modified Knotts<br />Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge<br />Centrifuge tube at 1300 to...
Stages of a Parasites Life Cycle <br />1.     oocyst<br />2.     Larvae<br />3.     nymph <br />4.     Pupa<br />5.     Ad...
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Final powerpoint parasites

  1. 1. LAB REVIEW<br />PARASITES<br />&<br />BLOOD CELLS<br />
  2. 2. Band Neutrophil<br />
  3. 3. Red Blood Cells<br />
  4. 4. Lymphocyte<br />
  5. 5. Eosinophil<br />
  6. 6. Platelets<br />
  7. 7. Basophil (in horses it is blue)<br />
  8. 8. Monocyte<br />
  9. 9. Segmented Neutrophil<br />
  10. 10. Tapeworm <br />
  11. 11. Hookworm<br />
  12. 12. Heartworm filaria<br />
  13. 13. Roundworm<br />
  14. 14. Coccidia<br />
  15. 15. Whipworm<br />
  16. 16. Mites<br />
  17. 17. Demodex<br />
  18. 18. Giardia<br />
  19. 19. FLEA<br />
  20. 20. Mite<br />
  21. 21. Babesia Canis<br />
  22. 22. Sucking Lice…………..Biting Lice<br />
  23. 23. Ehrlichia<br />
  24. 24.
  25. 25. American Dog Tick….……..Deer Tick<br />
  26. 26. Lone Star Tick………Rocky Mtn Wood Tick<br />
  27. 27. Dirofilaria immitum….dipeteloma reconditum<br />
  28. 28. Hemobartonella???<br />
  29. 29. Avian Blood<br />
  30. 30. Warble or Cuterebra (fly larvae)<br />
  31. 31. Anaplasma Marginale<br />
  32. 32. Lung Worm<br />
  33. 33. paragonimus kellicotti<br />
  34. 34. Lung Fluke<br />
  35. 35. Eucoleus boehmi<br />
  36. 36. Urine<br />Physical Exam:<br />Smell<br />Color<br />Turbidity<br />Foam<br />
  37. 37. Urine<br />Chemical Exam:<br />pH<br />Bilirubin<br />Protein<br />Occult Blood<br />Glucose<br />Ketones<br />Specific gravity<br />Nitrites<br />WBCs<br />
  38. 38. Heartworm Test<br />Buffy Coat method (hematocrit test)<br />Modified Knott’s technique<br />Filter membrane technique/Di-fil<br />Blood Smear<br />SNAP: Antigen testing does not detect the actual parasite but the host’s response to the disease<br />
  39. 39. Steps For <br />Modified Knotts<br />Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge<br />Centrifuge tube at 1300 to 1500 rpm for 5 minutes<br />Pour off liquid supernatant, leaving sediment at bottom of tube<br />Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain<br />Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective.<br />Filter Membrane<br />Mix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube<br />Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder<br />Connect syringe to filter holder and gently empty through filter holder<br />Fill syringe with water and repeat above step<br />Open holder, use thumb forceps to remove filter membrane<br />Place filter membrane on a clean microscope slide<br />Add one drop of stain, cover with coverslip, examine for microfilariae<br />
  40. 40. Stages of a Parasites Life Cycle <br />1. oocyst<br />2. Larvae<br />3. nymph <br />4. Pupa<br />5. Adult<br />

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