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GBM Cancer Cells Reserach  Report MCG 2008
 

GBM Cancer Cells Reserach Report MCG 2008

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This Presentation shows the effect of Cytoreg(R) in various Brian Tumor Cell lines. Performed at the Medical College of Georgia. Presented in April of 2008

This Presentation shows the effect of Cytoreg(R) in various Brian Tumor Cell lines. Performed at the Medical College of Georgia. Presented in April of 2008

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    GBM Cancer Cells Reserach  Report MCG 2008 GBM Cancer Cells Reserach Report MCG 2008 Presentation Transcript

    • Cytoreg® induced cytotoxicity in human brain tumors involves acute membrane damage Sangeetha Sukumari-Ramesh1, Nagendra Singh2, Suash Sharma2, John R. Vender1, Krishnan M. Dhandapani1 1Department of Neurosurgery and 2 Department of Pathology, Medical College of Georgia
    • FIGURE 1 (A) Effect of CytoReg (concentration response) on T98G, U87MG, T67, C6 glioma cells by MTT (and LDH – data not shown) (B) Cell Morphology 0.5h 1h 3h 6h 24h 48h 0 25 50 75 100 Control 1000X 500X 250X 100X 50X 25X 75X Treatment Time Cellviability(%ofcontrol) 0.5h 1h 3h 24h 48h 0 25 50 75 100 C ontrol 1000X 500X 250X 100X 50X 25X 75X Treatment Time Cellviability(%ofcontrol) T98G
    • 0X 250X 100X 50X 0 25 50 75 100 CytoReg CytoReg (pH 7.4) Dilution Cellviability(%ofcontrol)
    • Cytoreg vs. Cyto 0X 1000X 500X 250X 100X 50X 0 25 50 75 100 CytoReg CytoRegM Dilution Cellviability(%ofcontrol)
    • FIGURE 2 (A) Flow cytometry in T98G/U87MG – necrosis vs. apoptosis (B) Western of cleaved caspase3, DNA fragmentation T98G U87MG 0 25 50 75 100 125 Vehicle 250X CytoReg CytoReg + Z-VAD.fmk CytoReg + PFT- Cellviability(%ofcontrol)
    • FIGURE 3 Effect of CytoReg on non-transformed cells (neurons, astrocytes) Neurons Astrocytes 0 25 50 75 100 125 Veh 50X 100X 250X Cellviability (%ofcontrol)
    • FIGURE 4 (A) PI staining showing membrane disruption – 6h T98G (B) Membrane disruption assay (C) Add trypan blue staining 0x 250X 100X Data 1 0X 250X 100X 0 30 60 90 0X 250X 100X CytoReg %CellularMembrane Disruption 0X 250X 100X 0 25 50 75 100 CytoReg %TrypanBluePositive
    • FIGURE 5: KM D-456b Effect of CytoReg on BCNU-induced cell death in T 98G gliom a cells following a 72h treatm ent in serum 0 20 40 80 1 10 100 1000X CytoReg 500X CytoReg 0X CytoReg BCNU ( M) Cellviability(%ofcontrol) KM D-456 Effect of CytoReg on BCNU-induced cell death U87M G gliom a cells following a 72h treatm ent in serum 0 20 40 80 1 10 100 0X CytoREG 1000X CytoREG 500X CytoREG BCNU ( M) Cellviability(%ofcontrol) KM D-448 Effect of CytoReg in com bination w ith T em odar follow ing a 48h treatm ent in confluent T 98G glioblastom a cells 0 50 100 250 10 100 0X 1000X 500X * ** ** Temozolomide ( M) Cellviability (%ofcontrol)
    • FIGURE 6 Effect of CytoReg on primary brain tumors from patients – 3 GBM and 5 meningiomas - 24h 48h 0 25 50 75 100 Control 1000X 500X 250X 100X 75X 50X 25X Cellularviability(%ofcontrol) GBM #1 24h 48h 0 25 50 75 100 Control 1000X 500X 250X 100X 75X 50X 25X Cellularviability(%ofcontrol) GBM #2 24h 48h 0 25 50 75 100 Control 1000X 500X 250X 100X 75X 50X 25X Cellularviability(%ofcontrol) 24h 48h 0 25 50 75 100 Control 1000X 500X 250X 100X 75X 50X 25X Cellularviability(%ofcontrol) Men #1 Men #2