Bioinfo - Grad - 7
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Bioinfo - Grad - 7 Presentation Transcript

  • 1. + Bioinformática Mapeamento e Montagem de Transcritoma Gabriel da Rocha Fernandes Universidade Católica de Brasília gabrielf@ucb.br - fernandes.gabriel@gmail.com
  • 2. + Objetivos 2 nMontar os reads em transcritos (sem genoma referência) nIdentificar novos transcritos (genoma referência) nQuantificar a expressão dos genes.
  • 3. + Ferramentas nMontagem de novo n Trinity n Oases (Velvet) nMapeamento n Bowtie n BWA n Maq n TopHat 3
  • 4. + Do que preciso para o mapeamento nGenoma Referência: n Fasta (mapeamento) n GenBank ou GFF (quantificação) 4
  • 5. + Splicing alternativo 5
  • 6. + Fusão Genica 6
  • 7. + Quantificando 7