Green Pacific Biologicals              Junio 2012    Leandro Vetcher – CEO   lvetcher@greenpacificbio.com            @lvet...
GPB: Plataforma de ingenieria genetica                                        GPB Stable, stronger                      Mo...
GPB: Soluciones para biocombustibles        Increase carbon            fixa on                                            ...
Expresión génica nuclear mas fuerte GFP expression by Western blotting                 Accumulation of GFP by confocal mic...
Avg. fluorescence           per cell (au)    Avg. fluorescence      per cell (au)                             Expresión gé...
Mas transformantes expresando transgenes                      More rapid and efficient                           engineeri...
RNAi funcional- ingeniería metabólica     Genetic RNAi using GPB platform better than                 traditional mutant  ...
Localización subcelular de proteína                         Membrane             Lipid droplet                         loc...
Secreción de aceites no-toxica Non-toxic mechanism Secreted LDs spontaneously separates, sidestepping  harvesting/extrac...
Beneficios de secreción de aceites no-toxicaBASE CASE:                          GPB CASE:• Milk harvested by killing cow  ...
Modelado Tecno-económico      Oil productivity model + operational & capital costs• Physics: energy balanced,reasonable en...
Modelado Tecno-económico: valor de GPBSecretion• Enables continuous process through recycling of cells after secretion• Mo...
Process Flow Diagram Algae Biofuel Facility                                Base Case                                      ...
Modelado Tecno-económico                                          Base Case    0%     50%     90%                         ...
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Presentación leandro vetcher

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  • Stable transgene expression Consistent transgene expression Much higher level of transgene expression More expressing transformants Access to all cellular compartmentsRNAi functional Strain health and stability #2 Algae has the ideal characteristics for an ideal renewable energy platform.satisfy increasing energy demands + mitigate effects of climate change+ protect increasingly scarce natural resource Minimize/avoid competition with arable land for agriculture High oil productivity per acre (10x-100X over plants) Use waste water and saline water, reducing competition for limited freshwater supplies Recycle carbon from CO2-rich flue emissions from power plants and other industrial emitters
  • GPB rnai- gives benefits of starch knockout/mutant- more oil- without drawbacks on growth rate.
  • A NOTE ON MILK STORAGE CAPACITY: There is a limit to how much milk a cow can produce in one day, since they can only process a fixed amount of grass (energetically limited) If cows are milked daily, there is no benefit to having cows that can store more than one day’s worth of milkBASE CASE: Once grown, cow produces milk which is harvested by killing cow Majority of available energy (grass) goes into making cow GPB CASE: Milk is harvested by secretion from cow (milking) and cows returned to pasture Roughly 100% available energy (grass) goes into making milk (once cow population is established)
  • Presentación leandro vetcher

    1. 1. Green Pacific Biologicals Junio 2012 Leandro Vetcher – CEO lvetcher@greenpacificbio.com @lvetcher Green Pacific Biologicals - Not for distribution
    2. 2. GPB: Plataforma de ingenieria genetica GPB Stable, stronger More efficient Access to the gene expression engineering entire cell other 0 50 transformants 50 % expressing % expressing transformants other GPB 0 Only company with rapid and stable eukaryotic algae nuclear genetic engineering Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 2
    3. 3. GPB: Soluciones para biocombustibles Increase carbon fixa on Implement efficient Maximize oil secre on carbon flux into oil Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 3
    4. 4. Expresión génica nuclear mas fuerte GFP expression by Western blotting Accumulation of GFP by confocal microscopy GFP protein Cell extract Wild-type stds (ng) WT GPB GPB Strains 4
    5. 5. Avg. fluorescence per cell (au) Avg. fluorescence per cell (au) Expresión génica nuclear estable5
    6. 6. Mas transformantes expresando transgenes More rapid and efficient engineering Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 6
    7. 7. RNAi funcional- ingeniería metabólica Genetic RNAi using GPB platform better than traditional mutant Starch lower Oil higher Growth normal 7
    8. 8. Localización subcelular de proteína Membrane Lipid droplet localization localization Untransformed cell Membrane part+GFP Lipid droplet part+GFP 8
    9. 9. Secreción de aceites no-toxica Non-toxic mechanism Secreted LDs spontaneously separates, sidestepping harvesting/extraction Works by interactions between proteins on LDs and cell membrane or other cellular components Platform makes GPB uniquely positioned to implement oil secretion in algae 9
    10. 10. Beneficios de secreción de aceites no-toxicaBASE CASE: GPB CASE:• Milk harvested by killing cow • Milk harvested through secretion and cow returned to pasture• Majority of energy  Making cow • Majority of energy (grass) goes into making milk • If cows are milked daily, there is no benefit to having cows that can store more than one day’s worth of milkMILK + MILK ?
    11. 11. Modelado Tecno-económico Oil productivity model + operational & capital costs• Physics: energy balanced,reasonable energy inputs•Biology: realistic growth andenzymatic rates, cell densities, PSE• Interaction between variables(e.g., cell density affects totalenergy captured)• Pilot and commercial capex andoperational cost for PBRs and openponds (with Tryg Lundquist, PhD atCal Poly)
    12. 12. Modelado Tecno-económico: valor de GPBSecretion• Enables continuous process through recycling of cells after secretion• Most benefit through higher TAG Productivity• Elimination of several costly process steps in TAG recoveryOil metabolic engineering• Improved redirection of light energy into TAGs• Very high oil content levels not necessary with oil secretionOther• Identify additional bioengineering solutions• Prioritize R&D projects based on synergies and on relative impact in costValidationWe can reach $50/bbl with realistic performance metrics for each of ourgenetic engineering solutions
    13. 13. Process Flow Diagram Algae Biofuel Facility Base Case GPB ProcessMake-up Water Inoculation Ponds Recirculation Algae Biomass BlowdownMake-up Water High Rate Algae Gravity Settling Basin Ponds Thickener Algae Biomass CO2 Digestate Natural Gas Anaerobic Solar Drying Generator CH4 Digester Beds + CO2 Residuals Algae Biomass Unrefined Oil Solvent Based Flash Dryer Oil-Extraction Algae acts a catalyst that converts CO2 and sunlight into TAGs efficiently, without being consumed in the process Work with Tryg Lundquist, Assistant Professor at Cal Poly
    14. 14. Modelado Tecno-económico Base Case 0% 50% 90% 500 Cost per barrel ($/bbl) 400 300 200 100 0 0 5 10 15 20 25 30 35 40 45 50 55 60 % Oil (w/w)Early adoption of tecnoeconomic analysis provides:  Quantification of value of biofuel solutions  Guidance of R&D projects  Validation

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