6 introduction fruit breedomics mab services laurence francois
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6 introduction fruit breedomics mab services laurence francois

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6 introduction fruit breedomics mab services laurence francois 6 introduction fruit breedomics mab services laurence francois Presentation Transcript

  • FruitBreedomics Molecular Assisted Breeding transfer Pere Arus, Joan Bonany, François Laurens
  • Reminder: some results of apple and peach breeding questionnaires (2011-2012) Total answers: - Apple: 31 - Peach: 18 Current Bottlenecks in apple breeding programmes Fundings Time/ labour space Club variety pollen availability Available germplasm novel traits 10 5 6 1 1 Tests for resistance assessment Tests for quality assessment Widening genetic basis for resistance Markers for resistance genes Climatic factors 2 1 2 1 1
  • Reminder: some results of apple and peach breeding questionnaires (2011-2012) Are molecular markers being used currently in your breeding program? Yes: Apple: 43% (on 21) Peach: 31% (on 12)
  • What are molecular markers used for in your breeding program? Apple peach Parent selection 8 3 Hybrid selection Identification of hybrids or varieties 6 5 5 4 Identification of parentage 5 5 Genetic profile for intellectual property defense 2 3
  • What are the main reasons for not using molecular markers in the breeding programs? What are the reasons for not using molecular Apple markers in the breeding program ? No added value 6% Non economically viable 39% No training in usage of molecular markers for breeding purposes 11% No markers available for traits of interest 22% Peach No technology available 22% Non economically viable 44.4% Aim of FruitBreedomics = tackle these technical, economical, training issues No markers available for traits of interest 22.2% No training in usage of molecular markers for breeding purposes 33.3% No technology available ; 22.2%
  • What FruitBreedomics provided so far and will provide? Big genotyping SNP chips: peach IRSC=8K; apple: 20K, 420K => Fine mapping => find out markers close to the traits /Apple and peach – Major genes – QTLs => From various genetic backgrounds (PBA, GWA) Test high throughput and cheap genotyping tools adapted to Breeders needs YOUR Pilot  MABLOGO studies
  • FruitBreedomics MAB Services Proposal YOUR LOGO
  • Major challenges faced by fruit breeders on the use of MAB Fruit Breeding companies = small size companies => limited access to expertise in genetics, genomics, marker technologies,... => Lack of lab facilities for molecular analysis, bioinformatics support,... FruitBreedomics needs to: - address the specific problems of each company concerning the use of markers in their breeding programs - preserve confidentiality on parents, breeding strategies, key characters, etc. FruitBreedomics planned to provide breeders with support on MAB - Pilot study - Training sessions YOUR LOGO => A more individualized support was needed
  • Transfer technology to stakeholders GENOTYPING PLATFORM KNOWLEDGE PROVIDERS (PARTNERS) Advice on potential use On site visits FRUITBREEDOMICS On site assistance MAB Sampling SERVICES Support decision Data analysis Companies with experience on MAB transfer ….
  • What FruitBreedomics MAB Service should be • A service offered on a commercial basis • Offered to FruitBreedomics stakeholder breeding programs in Rosaceae species • Using FruitBreedomics (and other published) generated information • Not a genotyping Platform YOUR LOGO
  • Transfer technology to stakeholders Implementation GENOTYPING PLATFORM KNOWLEDGE PROVIDERS (PARTNERS) FRUITBREEDOMICS MAB SERVICES >2015 2014 Végép olys /INRA FEM IRTA ….