5 mab peach breeding arus pere
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5 mab peach breeding arus pere






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5 mab peach breeding arus pere 5 mab peach breeding arus pere Presentation Transcript

  • 1. PARENT CHARACTERIZATION AND SELECTION • • • Relationships between parents Cross planning  Genetic distances  Heterozygosity Breeder’s rights enforcement 2. SEEDLING MARKER-ASSISTED SELECTION YOUR LOGO
  • MARKER (SSR) FINGERPRINTING FOR BREEDER’S RIGHTS PROTECTION 251 261 280 280 246 280 251 246 261 261 189 196 139 139 189 196 189 196 196 189 196 YOUR LOGO 135 148 148 148 139 135 139 148 View slide
  • PEDIGREE VALIDATION 167 P1 P2 141 178 183 147 189 178 183 141 H-A 178 H-B 189 125 141 YOUR LOGO 147 147 129 131 125 131 125 131 View slide
  • SELECTING PARENTS FOR A CROSS: GENETIC DISTANCE The DENDROGRAM Clusters group genetically similar parents Do my pedigree data fit? Do I need more variability? Do I want to have a fixed background where only a few traits segregate?
  • SELECTING PARENTS FOR A CROSS: HETEROZYGOSITY % Heterozygosity 0 10 20 30 40 50 60 More heterozygosity in the parents  More segregation in their progeny
  • MARKER-ASSISTED SELECTION G M1 M2 APPROPRIATE USE OF MARKERS REQUIRES DETAILED KNOWLEDGE OF THE PARENTS OF EACH BREEDING PROGRAM 0-5 cM LINKED MARKERS: Require validation in a progeny (phase knowledge) DIAGNOSTIC MARKERS: Presence of certain alleles predict corresponding phenotypes YOUR LOGO
  • a t D h ncia ell H RINE g PEACH MAJOR GENES used for Marker- Assisted Selection (MAS) Male sterility Acidity Peach/Nectarine Pubescencia de la pell MELTING NECTARINE Color de la polpa Consistencia de la polpa PEACH Flesh color NON-MELTING WHITE YELLOW G/g Y/y Forma del fruit Flesh texture Consistencia de la polpa M/m Fruit shape Pubescencia de la pell Consistencia de la polpa Forma del fruit PEACH MELTING ROUND NECTARINE Color de la polpa NON-MELTING Color de polpa MD MELTING ROUND WHITE WHITE Maturity date NON-MELTING NON-MELTING M/m F/f FLAT Sh/sh YELLOW Y/y YOUR LOGO G/g M/m FLAT Sh/sh YELLOW Y/y
  • Marker Assisted Breeding (MAB) workflow - ARE THERE MARKERS AVAILABLE FOR THE TRAIT? YES NO (Ex: acidity, flat shape…) (Ex: pistilar closing…) Use the marker or saturate the region in a segregating population to find a better marker Find or develop a segregating population and construct a genetic map and look for the position of MAJOR GENES or QTLs Prunus reference map (Dirlewanger et al. 2004, PNAs 101(26):9891-9896) validate markers in germplasm collection Peach genome (www.rosaceae.org) MAS YOUR LOGO
  • INTRODUCTION of MAB in the IRTA BREEDING PROGRAM 1st STEP: DEMONSTRATION: plants in the field phenotype known or that could be determined # plants analyzed by fruit shape # plants analyzed by acidity Years 1,311 1,349 2010-2012 Less than 5 % of errors in phenotype predictions with either marker 2nd STEP: SEEDLING SELECTION: selection in the greenhouse # plants analyzed by fruit shape # plants analyzed by acidity Years 1,245 1,502 2012-2013 66% of the seedlings of the program in 2013 were selected with markers: All except small progenies and fixed progenies YOUR LOGO