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28 new traits  in advanced breeding populations-a. peil
 

28 new traits in advanced breeding populations-a. peil

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    28 new traits  in advanced breeding populations-a. peil 28 new traits in advanced breeding populations-a. peil Presentation Transcript

    • New traits in advanced breeding populations in apple and peach WP2 – Establishment of prebreeding material Results achieved since the beginning of the project and plans for 2013
    • Reminder of the main objectives of the WP • Description of possible donors • Introduction of new traits in to apple and peach • Combination of traits • Pyramiding of resistance traits • Application and improvement of the fast breeding approach  Main aim is to develop advanced material with new traits for the breeders
    • Results achieved since the beginning of the project • Task 2.1: Description and evaluation of available plant material regarding the traits powdery mildew, brown rot –peach, scab resistance (Rvi11, Rvi12), fire blight resistance (Evereste, Mr5) material with pyramided powdery mildew resistances (Pl1Pl2Pld, Pl1Pl2Plm) Powdery Mildew Brown Rot
    • Selection of parents to increase resistance/tolerance to brown-rot BO 92038173 (Contender x Elegant Lady) Gialla tardiva Percoca di Romagna 7 MA 25-13-143 (Suncrest X UCD-F8,5-156 ) IFF 638 (Bea x Aline) New possible parents (also with putative resistance to PPV) MA 25-10-111 ((Romea*Snowqueen) X SD 75)
    • Results achieved since the beginning of the project • Task 2.2 Conventional pre-breeding – crosses were performed to introduce new traits, combine and or pyramid resistance genes, quality Number of crosses Pollinated flowers Seeds* Seedlings* Peach 44 1609 Apple 40 7498 7474 2803 * Not all seeds and seedlings from crosses 2012 are included
    • Traits used in apple and peach Apple Peach Introduction of new traits Introduction of new traits Rvi11 Vbj brown rot resistance Rvi12 Vb brown rot tolerance FbE Combining and pyramiding of traits FbR5 Rm1, Rm2 Green peach aphid Low chilling Vr1, Vr2 Powdery mildew Combining and pyramiding of traits maturity time Rvi2, Rvi4, Rvi6 Vh2, Vh4, Vf Y yellow/white flesh FbF7 D acid/subacid Pl1, Pl2, Pld, Plm G peach/nectarine Sd1 S flat/round increase quality F melting/non melting
    • Examples for apple Comparison of Rvi4 symptoms with presence or absence of marker CH02C02a 23% 29%20% 28% Marker for Rvi11 Marker for Sd1 Marker for Rvi11 and Sd1 None of the markers Quality, Sd1 x Rvi11
    • Results achieved since the beginning of the project • Task 2.3: – Application and improvement of the fast breeding approach • Introduction of fire blight resistance in to apple – Evereste – Malus xrobusta 5 • Establishment of early flowering lines – With the transgene on another LG – Different cultivars with the early flowering gene – Use of a inducible promoter and a different flowering gene
    • JKI, Dresden, Germany Overexpression of the transcription factor BpMADS4 from silver birch (Betula pendula Roth.) in transgenic line of Pinova (T1190) induced precocious flowering (in vitro, and also few months after transfer to greenhouse) Flachowsky et al., 2007 Plant Breeding Silver birch Pinova_T1190 BpMADS4 The fast breeding approach
    • The idea behind the approach Flachowsky H, Le Roux P-M, Peil A, Patocchi A, Richter K, Hanke M- V. (2011) Applying a high-speed breeding technology to apple (Malus × domestica) based on transgenic early flowering plants and marker-assisted selection. New Phytologist 192, 364-377 Le Roux P-M, Flachowsky H, Hanke M-V, Gessler C, Patocchi A (2011) Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from ‘Evereste’. Molecular Breeding, in press.
    • Fire blight Fotos: http://www.feuerbrand.it/info/infos/krankheitssymtome/bluetenbefall.html; http://www.hortipendium.de/Feuerbrand; http://www.fohnsdorf.at/cms/umwelt/feuerbrand.asp; http://www.ogv-teisendorf.de/Media/Feuerbrand%20Triebspitze.jpg; http://www.landwirtschaft.sg.ch/home/landwirtschaftliches/Beratung/Pflanzenbau/Pflanzenschutz/Feuerbrand_/Allgemeine_I nformationen.html
    • Fire blight solutions Foto: http://www.stadt-lienz.at/system/web/zusatzseite.aspx detailonr=218784046 Foto: http://tirol.orf.at/news/stories/2543474/ Foto: http://www.nzz.ch/aktuell/schweiz/null-toleranz-bei-feuerbrand-1.17954557 Foto: http://www.gartentipps.com/feuerbrand- melden-es-besteht-meldepflicht.html
    • Destroy all infected material Foto: http://www.nzz.ch/aktuell/schweiz/null-toleranz-bei-feuerbrand-1.17954557
    • Fb_E Major QTL identified in the ornamental apple Evereste on the bottom of Linkage Group (LG) 12 (R2 = 50-70 %). Durel et al., 2009 – Genome Parravicini et al., 2011 – Molecular Plant Pathology General objective: selecting apple pre-breeding genotypes carrying major genes/quantitative trait loci (QTL) for disease resistance from wild apples, with as less as possible „wild“ genome.  Introgression of a highly efficacious fire blight (FB) resistance locus Fb_E from Evereste. Using T1190 at Agroscope ACW Introduction of fire blight resistance from Evereste
    • X X X X X EveresteT1190 F1 3 Fb_E + BpMADS4 Topaz, Diwa® and Maloni Sally ® Royal Gala GrannySmith Braeburn2013 2010 2011 End 2008 beg. 2009 2012 BC‘1 9 Fb_E + BpMADS4 BC‘2 13 Fb_E + BpMADS4 BC‘3 ? Fb_E + BpMADS4 BC‘4 ? Fb_E without BpMADS4 2014 2018 first flowers of this pre-breeding material 2012/2013 111 seeds were harvested and stratified = 25 % potential BC’3 plants State of the art
    • We started with one early flowering Pinova line carrying the transgene on LG 4 To enlarge the applicability of the approach three Pinova lines carrying the BpMADS4 on different linkage groups (LG) than LG 4 were created 17 Line Genotype Linkage group Integrated vector backbone 1189 PN 8 None 1236 PN 16 None 1240 PN 14 Ca. 1700 bp after BL (tests outstanding) Improvement of the fast breeding approach
    • Other cultivars carrying the early flowering gene • Successful transformation with early flowering genes under control of 35S promotor in different apple cultivars • Generation of transgenic lines of different apple cultivars overexpressing BpMADS4 under control of 35S cv. ‘Pinova’:3, ‘Gala’: 5, ‘Santana’: 1, ‘Gala Mitchgla’: 1 • Transgenic lines flowered during in vitro cultivation 18 GA 35S BpMADS4 SAN 35S BpMADS4 PN 35S BpMADS4 vitro cultivation of transgenic apple lines. Early flowering apple plants during in vitro cultivation. Improvement of the fast breeding approach
    • What else? • Establishment of early flowering lines using a new gene (TFL1) under the control of an inducible promoter Pinova with TFL1 under HSP
    • Publications Flachowsky H, Le Roux P-M, Peil A, Patocchi A, Richter K, Hanke M-V. (2011) Applying a high-speed breeding technology to apple (Malus × domestica) based on transgenic early flowering plants and marker-assisted selection. New Phytologist 192, 364-377 Le Roux P-M, Flachowsky H, Hanke M-V, Gessler C, Patocchi A (2011) Use of a transgenic early flowering approach in apple (Malus × domestica Borkh.) to introgress fire blight resistance from ‘Evereste’. Molecular Breeding, in press.
    • Main challenges for 2013 • Identification of new traits to be introduced in to apple and peach • Selection of parents for the next crosses (phenotyping for brown rot) • Development of early flowering lines with TFL under the control of a inducible HeatShockPromoter (HSP)
    • The future What are you expecting from us?  New traits  New combinations  New genes Acknowledgements: Melanie Jänsch, Markus Kellerhals, Lucie Leumann, Andrea Patocchi, Alessandro Liverani, Thierry Pascal, Kathleen Barthel, Henryk Flachowsky, ...