13 peach characteristics of prebreeding material pascal thierry

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13 peach characteristics of prebreeding material pascal thierry

  1. 1. Characteristics of the Pre-Breeding Parent Lines Developed in Peach green peach aphid, powdery mildew, brown rot, non-acid-flesh, blood-flesh, … YOUR LOGO
  2. 2. Develop advanced material with new traits for peach breeders ¿Which new traits are targeted to INRA-Avignon? Traits of resistance to: - green peach aphid - peach powdery mildew - Plum Pox Virus (M strain) - brown rot (Monilinia laxa) - nematodes Traits of “fruit quality” as: - blood-flesh (DBF and bf) - high-content in 2ary metabolites Traits of the tree habit as: - weeping shape - semi-double flower so far little attention …
  3. 3. Develop advanced material with new traits for peach breeders Characteristics of resistance sources ... qualitative/quantitative green peach powdery aphid mildew plum pox virus brown rot Weeping Flower Peach Rm1 - - - Rubira Rm2 - - - Malo Konare - Vr1 - - Pamirskij 5 - Vr2 - - BC2 from P. davidiana - - - ongoing (WP5) Bolinha - - - ongoing (WP5) P. davidiana MP.SD-3.1 MP.SD-5.1 PM.SD-6.1 PM.SD-8.1 - P. dulcis ongoing ongoing PPV.RD-1.1 PPV.RD-2.1 PPV.RD-7.1 ongoing ?
  4. 4. Develop advanced material with new traits for peach breeders Characteristics of resistance sources ... first by classical phenotyping green peach aphid powdery mildew plum pox virus Weeping Flower Peach Rubira Malo Konare Pamirskij 5 BC2 from P. davidiana TIC (Test with Inoculation on Cultivar) Bolinha P. davidiana P. dulcis TIR (Test with Inoculation on Rootstock) brown rot
  5. 5. Develop advanced material with new traits for peach breeders Characteristics of resistance sources ... then mapped on genome GL1 GL2 PPV.RD-1.1 resistance to PPV Y/y white/yellow flesh color PPV.RD-2.1 resistance to PPV RMia resistance to nematodes bf blood-flesh color pl weeping habit SD-MP.3.1 resistance to green peach aphid GL6 GL5 GL4 GL3 GL7 GL8 Vr2 resistance to peach powdery mildew DBF blood-flesh color D non-acid flesh SD-MP.3.1 resistance to green peach aphid SD semi-double flower SD-PM.6.1 resistance to powdery mildew PPV.RD-7.1 resistance to PPV SD-PM.8.1 resistance to powdery mildew g glabrous skin S* flat fruit Rm2 resistance to green peach aphid Peach genetic linkage map (8 chromosomes) SSR, SNP-KASP® molecular markers available for MAB for most of these traits
  6. 6. Develop advanced material with new traits for peach breeders Characteristics of the peach species ... growing cycle very short - March 2014: crosses - June to September: harvest and statification in cold chamber (or 6 BAP) - Winter 2014/2015 : early selection screening under greenhouse conditions or by Marker molecular Assisted Selection … - Springtime & Summer 2015: growing of resistant genotypes selected previously - March 2016: new crosses with flowers coming from resistants genotypes … according objectives, one generation all two years is easily feasible in peach
  7. 7. Develop advanced material with new traits for peach breeders Characteristics of the peach species ... growing cycle very short example with P. davidiana and the resistance to pow. mildew x P. davidiana Summergrand sssssssSummergrand 1st generation 2nd generation “fast breeding” in peach (3 generations / 6 years) with a rapid progress on fruit traits … 3rd generation
  8. 8. Develop advanced material with new traits for peach breeders Characteristics of the pre-breeding parent lines … - di-allele crosses between resistant genitors and recurrent parents as Summergrand … 100 to 200 individuals by combination stored in conservatory -flat fruit or blood-flesh (DBF) pre-selections bearing 1 / 2 single resist. genes (Rm1 / Vr1) - crosses realized to associated different resistance factors to green peach aphid powdery mildew … in the aim to improve the durability of these resistances

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