Procedure
Upcoming SlideShare
Loading in...5
×
 

Procedure

on

  • 3,295 views

 

Statistics

Views

Total Views
3,295
Views on SlideShare
3,234
Embed Views
61

Actions

Likes
0
Downloads
20
Comments
0

1 Embed 61

http://www.burroughscience.moodlehub.com 61

Accessibility

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Procedure Procedure Presentation Transcript

  • Observing human cellsand plant cells
    What are the differences?
  • How this presentation works
    While the screen is BLUE, watch and listen
    When the screen is YELLOW, do what the instructions tell you
    You will need to watch some videos during this lab, they are on the same page of the website, just press escape to get out of full screen mode.
  • Your objective
    At the end of this lab, you will have seen real animal cells from your own body and plant cells from an onion and will be able to list the differences between them.
  • Set up your notebook
    Write a title: “Observing cells under the microscope”
    Divide the page into two halves. Label one “Observing onion cells” and the other “Observing cheek cells”
    Draw three circles in each section and label them:
    Low power
    Medium power
    High power
  • Equipment
    You will use a microscope for this lab. Take out the Parts of the Microscope card and watch the Using a Microscope video on the class website.
    Fill in the blanks.
    If you need to, you can pause and rewind the video.
    Glue the finished worksheet into your notebook
  • Safety
    You will be using biological specimens – there is always a risk of spreading disease or poisoning in a lab – especially when using biological specimens like human cells.
    Wash your hands and dispose of used Q-Tips immediately in the waste bin.
    Be careful while cutting onion.
    The stains we are using will stain your skin and clothes – wear an apron and gloves whenever you are handling the stain or slides. TAKE CARE.
  • Preparing the slides
    You will prepare two slide to look at under the microscope:
    One from your own cells, taken from the inside of your cheek
    One from an onion
  • Onion Cells - Procedure
    Remember, this is a BLUE slide, just read the instructions this time
    Obtain a clean slide and cover slip.
    Take a piece of onion and cut a piece about as big as your thumbnail.
    Using the forceps, carefully peel of the very thin skin between the onion layers and put it in the middle of your slide.
  • Onion cells – continued.
    Using the dropper, put a drop of Iodine stain on a slide.
    Be careful, stains can ruin clothing and other materials.
    Wait 3-4 minutes before putting a coverslip on the slide. Prepare your cheek cell slides while you wait.
    Put a cover slip over the stain. Do not press or you might burst the cells.
  • Cheek cells - procedure
    Obtain a clean slide and cover slip.
    Gently rub the end of a Q-tip on the inside of your mouth along the cheek. Some of the cells from your cheek will come off on the Q-Tip.
    Be careful! Do not gouge out your cheek.
    Wipe the Q-Tip on the center of the slide so that the cells from your cheek are transferred to the slide.
  • Cheek cells – continued
    Using the dropper, put a drop of Methylene Blue stain on a slide.
    Be careful, stains can ruin clothing and other materials.
    Immediately, throw the Q-tip away.
    Put a cover slip over the stain. Do not press or you may crush the cells.
  • Review the procedure.
    Now watch the Preparing Slides of Cheek and Onion Cells video on the class website
  • Prepare onion cell slide
    Obtain a clean slide and cover slip.
    Take a piece of onion and cut a piece about as big as your thumbnail.
    Using the forceps, carefully peel of the very thin skin between the onion layers and put it in the middle of your slide.
  • Onion cells – continued.
    Using the dropper, put a drop of Iodine stain on a slide.
    Be careful, stains can ruin clothing and other materials.
    Wait 3-4 minutes before putting a coverslip on the slide. Prepare your cheek cell slides while you wait.
    Put a cover slip over the stain. Do not press or you might burst the cells.
  • Cheek cells - procedure
    Obtain a clean slide and cover slip.
    Gently rub the end of a Q-tip on the inside of your mouth along the cheek. Some of the cells from your cheek will come off on the Q-Tip.
    Be careful! Do not gouge out your cheek.
    Wipe the Q-Tip on the center of the slide so that the cells from your cheek are transferred to the slide.
  • Cheek cells – continued
    Using the dropper, put a drop of Methylene Blue stain on a slide.
    Be careful, stains can ruin clothing and other materials.
    Immediately, throw the Q-tip away.
    Put a cover slip over the stain. Do not press or you may crush the cells.
  • Using the microscope
    Switch on the microscope lamp.
    Adjust the Diaphramso that the right amount of light is shining on the slide
    Using the Course Focus knob, move the microscope stage so that it is as high as it will go.
    Turn the microscope Nose so that the Low Power Objective lens is in use.
    Put the slide onto the microscope stage and fix with the Stage Clips.
    Use the Course focus knob to move the stage down until the cells are in focus.
    Ask the teacher to check your focus
    Draw your observations in one of the Low Power circles in your notebook.
  • Medium power
    Turn the microscope Nose so that the Medium Power Objective lens is in use.
    Adjust the focus with the Fine Focus knob.
    Ask the teacher to check your focus
    Draw your observations in the Medium power circle in your notebook.
  • High power
    Turn the microscope Nose so that the High Power Objective lens is in use.
    Adjust the focus with the Fine Focus knob. DO NOT USE THE COURSE FOCUS KNOB.
    Ask the teacher to check your focus
    Draw your observations in the High power circle in your notebook.
  • Analysis
    Look at the observations you recorded in your notebook.
    What differences do you see between the animal and plant cells?
    Write notes of the differences in your notebook.
  • Reflection
    Write a heading in your notebook, “Reflections”.
    What did you enjoy most about this lab?