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monsanto 08-23-05b

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  • 1. Corn Breeding Overview Ankeny Investor Field Day August 22, 2005 Mark Messmer, Corn Commercial Breeding Lead Monsanto Company
  • 2. MON Corn Breeding Keys To Success • Structured to support commercial strategy – Multiple channels Line Review Worksheet • Automating the Breeding 08_01DKD2*2/7640:@>0003.1001. Show selected lines only Export Add Filter? Lines Nursery notes YLD % of Check Keep Plot Lineage Mean Select Pedigree Trait 1 Trait 2 Trait 3 Comments x 08_01DKD2*2/7640:@>0003.1001. 110 14011 :@>0003.1001.4001. 7 5 22 nice Process 08_01DKD2*2/7640:@>0102.1001. 95 X 14012 :@>0003.1001.4002. 6 6 24 better 08_01DKD2*2/7640:@>0126.1001. 85 Breeder Comments Advance this line into PCM1 on 6 testers x 08_01DKD2*2/7640:@>0160.1002. 120 08_01DKD2*2/7640:@>0171.1003. 101 x 08_01DKD2*2/7640:@>0175.1002. 105 08_01DKD2*2/7640:@>0179.1003. 87 Data GCA SCA Checks Check mean Select Below and update above? Line Data Double click entry to show Set data sheet – Driving experimental product Select Set Entry Tester Gen # Loc SELIN YLD MST TWT YM RTLP STLP PHT EHT CZ INT FSP DRP ERM GCA All 39 120 120 105 97 120 220 105 100 75 75 100 114 X C7201 5 19HGZ1 F4 13 140 250 18.5 56.3 14 15 115 53 1 99 113 C8202 4 80IDM2 F4 13 103 229 19.1 56.7 12 2 116 44 3 4 100 114 X C8207 12 LH283 F4 13 113 226 19 57 12 1 118 42 5 4 100 115 C8101 26 :@>0003+ F3 7 132 241 18 56 13 6 112 51 2 99 113 numbers game Rank Data Ranks Data Set Entry Tester Overall Loc 1 Loc 2 Loc 3 Loc 4 Loc 5 Loc 6 Loc 7 Loc 8 Loc 9 Loc 10 Loc 11 Loc 12 Loc 13 Loc 14 Loc 15 Sort by Yield Levels Mean Yield 140 150 160 165 170 175 180 185 190 200 210 215 300 C7201 5 19HGZ1 1 45 55 66 33 20 5 4 4 3 2 2 1 2 Green - Hybrid above chk m C8202 4 80IDM2 18 50 66 33 21 30 40 5 6 7 8 2 1 3 Red - Hybrid below chk mea C8207 12 LH283 16 60 12 20 30 8 8 5 4 10 12 2 8 4 • Germplasm Diversity C7201 5 19HGZ1 2 1 5 8 25 47 36 22 1 1 5 4 3 8 C8202 4 80IDM2 4 2 5 22 44 56 12 11 5 8 22 14 16 55 C8207 12 LH283 16 14 22 11 10 1 17 27 26 5 4 9 8 7 – Broad and deep • Marker Assisted Line Development – An SOP Enhancing the probability of breeding success • Pipeline Depth – More improved products 2
  • 3. Corn Breeding Structure is Designed to Support Multiple Commercial Channels 1 0.9 Linkages and Commercial Development 0.8 0.7 Exchange of information 0.6 Line Development 0.5 Are key to Success. 0.4 Breeding Technology 0.3 0.2 0.1 0 LINE New BREEDING COMMERCIAL CHANNEL DEVELOPMENT Technology TECHNOLOGY BREEDING DEPLOYMENT Breeding • Marker Labs • F1 to national • Product • MON Brands MANAGEMENT • Statistics Characterization testing PRODUCT • National Partners • Disease and Insect • Quality grain • Channel Tech • Regional Partners Support programs • Winter Nurseries • Local Partners • Testing • Discovery Breeding • QA/QC • Channel Strategy • Channel Needs • Comm Development 3
  • 4. Regional Breeding Teams Tie Breeding Functions Together North W. Central E. Central South Regional Breeding Team Objectives: 1. Focus on most elite germplasm 2. Maintain important germplasm types 3. Enhance Product diversity 4. Enhance breeder germplasm knowledge and info exchange 5. Breeder synergy to identify and support customer needs 4
  • 5. Expansive breeding and testing geography enables effective product identification and placement 5
  • 6. State of the Art Automated Experimental Seed Processing Sets the Stage for Outstanding Field Data Quality Shelling Grading Waste Discard Color Sorting Finished Product Seed Treatment Culls and Keeps Ready to Packet 6
  • 7. Investment in State of the Art Field Plot Equipment Enables Direct Mimicking of Farmer Practices and Timing ~50% Increase in Plot Testing Capacity Over the Past Two Years 7
  • 8. Plant Breeding: What do we actually do? • Plant Breeding – is the science, art, and business of improving plants for human benefit. • Easy Description – Cross good germplasm by good germplasm – Generate a series of genetically different exptl. parents – Pick the right tester – Select the best experimental line via extensive field testing • More complicated Description delta(G)=1/C(0.25*k1*V(a)1.2/sqrt(V(P)1.2) + 0.25*k2*V(a)2.1/sqrt(V(P))2.1) 8
  • 9. Vast germplasm resources enable deployment of differentiated product portfolios 12 countries and 3 companies/country = 36 major germplasm acquisitions Asgrow DEKALB Int’l Int’l Asgrow USA DEKALB USA Inter-company Crosses Cargill Int’l Holden’s Agroceres Sensako 50% of breeding pop’n are “inter-company” crosses 9
  • 10. The Dilemma: Germplasm Sources X Genes 1 2 3 4 5 6 7 8 9 10 Corn has many thousands of genes, hundreds of which probably affect yield Over 3 billion different genotypes result from just 2 forms of each of 20 genes Yield from Parent 1 Yield from Parent 2 10
  • 11. Marker-Assisted Recurrent Selection (MARS) rapidly increases the frequency of favorable genes Breeder 1.0 F requency of F avorable G ene Selection and Tissue 0.9 Recombination Sampling 0.8 0.7 0.6 0.5 0.4 Data C0 C1 C2 C3 Multiple DNA extraction Analysis cycles Multiple cycles of MARS increases the frequency of favorable marker alleles associated with Generate Marker Fingerprint agronomic traits Genotyping Lab
  • 12. Marker Assisted Breeding: Sorting the Germplasm and Genes…A Vastly Enhanced Numbers Game Mapping QTL position, size, and favorable parent Yield from Parent 1 Yield from Parent 2 Fr eq u en cy o f : Cycle o f A Favo r ab le Th e Best Select io n A llele Gen o t yp e 0 0 .5 1 2 p er t r illio n Start 1 0 .6 2 5 p er b illio n 2 0 .8 1 1 p er 5 0 0 0 3 0 .9 6 1 in 5 End *Assumes 20 independent regions and 5% selection intensity 12
  • 13. Breeders Build an Optimized Genetic Model for Every Breeding Population and use MARS to Toward the Model Select Traits Quality metrics Predicted Model Response Parental Contribution Data Data Decisions Identify and Select QTLs RUBICON: Integrate and Analyze Field and Marker data 13
  • 14. Marker Characterization Early in the Breeding Enhances Probability of Success Late in the Process Genotypic and phenotypic X Year 1 selection of parents Recombine genes 6- to 8-year process 6- to 8-year process Marker Characterization 120,000+ Select best based on ted phenotype sis ction As er- t Sele rk Ma urren Yield test c Re Years 2-6 Select best Yield test 10’s Year 7 Commercial product success rate < Commercialize 1% 14
  • 15. Marker Assisted Breeding Drives Increased Genetic Gain 1.8 1.6 2x Improvement MTI value (Parent = 0) 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 2002 2003 2004 All Years Conventional Markers MTI = Multiple Trait Index that combines multiple traits like grain yield, grain moisture, standability, and test weight. 15
  • 16. 16 85 90 95 100 105 110 115 120 85 90 95 100 105 110 115 120 D 40 K4 D 57 K3 D C70 K 5- 1 D C 62 K 4- 6 N 50N R A6 5 R 1 D C 44 K 4- 6 N 50 B5 1 D C 71 K 4- 0 N 50 B9 1 Denotes YG hybrid N 50 C3 1 N 40 C5 4 N 50 C3 5 N 40 C7 2 N 50 C3 8 N 40 C5 5 N 51 C3 0 MON New 2005 Commercials N 40EA C5 1 Z 1 95 RM N 50 C4 6 105 RM N 50 B4 5 N 40 B6 1 N 50 C5 2 N 40 B7 3 N 50 C6 7 N 40 C6 3 N 50 C7 2 N 50 C7 6 N 40 C9 2 P N6 0 I 3 B8 O P N8 2 I 3 A4 O A full pipeline enables P N5 5 I 3 Y4 O P N8 2 I 3 A5 O P N5 6 I 3 Y5 O MON 2005 PCM4s 85 90 95 100 105 110 115 120 85 90 95 100 105 110 115 120 continued support of growth D C14 K 5- 3 DC01 K6- 5 MON Brand Leader hybrids R70 X4 D 57 K3 DC01 K6- 9 N 40 B6 2 R78 R G X1R/Y N60 C14 N 40 C8 1 N50 C81 N 50N R B1 1 R 1 N50 C82 N 50 C2 5 N50 C88 ` N50 C89 N 50 C2 8 N50 C92 via the commercial strategy (95 - 109 RM) Competitive Leader Hybrids N 50 C2 9 N50 C93 100 RM 108-109 RM N50 C97 N 51 C2 0 N51 C90 N 50 B1 1 P N4 3 I 3H1 O P N8 6 I 3 H7 O P N4 9 I 3M O 4 P N4 9 I 3M O 5 P N6 7 I 3 N0 O P N3 4 I 3N4 O
  • 17. 17 85 90 95 100 105 110 115 120 85 90 95 100 105 110 115 120 D C01 K 6- 5 D C3 0 K 6 -5 R70 X4 D C3 9 K 6 -7 D C01 K 6- 9 D C3 8 K 6 -7 R78 R G X1 R /Y D C4 0 K 6 -1 N 60 B1 4 N 60EA B62 Z 1 N 60 B1 6 N 60 C67 N 60 C0 9 ` N 61 C2 0 N 60 C68 N 61 C2 3 N 60 C69 116-117 RM N 61 C2 4 N 61E 1 C 6 1 ZA N 61EA C2 5 Z 1 110-112 RM P N1 9 IO 3 G 8 P N4 3 I 3 H1 O New commercial and P N3 3 IO 3 D 1 P N4 9 I 3 M4 O P N3 5 IO 3 B 1 P N4 9 I 3 M5 O P N3 4 I 3 N4 O P N3 6 IO 3 P 7 experimental products appear 85 90 95 100 105 110 115 120 DKC63-50 DKC63-79 DKC63-78 DKC64-10 NB6402NRR1 NB6504EZA1 NC6304 NC6307 MON 2005 PCM4s Denotes YG hybrid NC6313 NC6315 NC6403 MON Brand Leader hybrids Competitive Leader Hybrids NC6407 MON New 2005 Commercials NC6505 NC6509 NC6510 ready to allow growth in later RMs (110 - 117 RM) PION33B50 113-115 RM PION33D31 PION33B51 PION33P67
  • 18. Invention is the Best Way to Predict the Future Yield Test Weight Total Damaged Plants Stay Green (5 log(pvalue) units between marks) Rind Penetrometer Protogyny Plant Height Moisture Intactness GDU pollen shed Ear Height Gray Leaf Spot Chrom Markers 18 (10 cM between marks)