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textile biodegradation

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  • 1. Textile effluent biodegradation potentials of textileeffluent-adapted and non-adapted bacteria Olukanni O. D.*, Osuntoki, A. A. and Gbenle, G. O. Presented by FAISAL
  • 2. INTRODUCTION• Industrialization is vital to a nation’s economy• There are associated problems resulting from the introduction of industrial waste products into the environment.
  • 3.  The textile industries produce effluents that contains Dispersants Leveling agents Acids Alkalis carriers and various dyes (Cooper,1995.)
  • 4. INTRODUCTION• In many Nigerian cities, the textile factories daily discharge millions of litres of untreated effluents in the forms of wastewater. (Olayinka and Alo, 2004)• This alters the• pH,• biochemical oxygen demand (BOD)• chemical oxygen demand (COD), (Ajayi and Osibanjo, 1980).
  • 5.  Several methods are used in the treatment of textile effluents filtration, specific coagulation, use of activated carbon and chemical flocculation. Some of these methods are effective but quite expensive(Do et al., 2002; Maier et al., 2004).
  • 6.  Biotreatment offers a cheaper and environmentally friendlier alternative for color removal in textile effluents. The ubiquitous nature of bacteria makes them invaluable tools in effluent biotreatment.
  • 7.  The chemical nature of dyes varies, but azo dyes are the most widely used. The decolourization of azo dyes has been found to be effective under anaerobic conditions. The anaerobic degradation yields aromatic amines which are mutagenic and toxic to humans and cannot be metabolized further under the conditions which generated them (Chung and Stevens, 1993; Do et al.,2002).
  • 8.  To exploit the biodegradation abilities of our indigenous microbial flora for remediative purposes. The study is aimed to discovering isolates with the potential for use in biological treatment of textile effluents.
  • 9. MATERIALS AND METHODS
  • 10. Sources of organism• Textile effluent-adapted bacteria were isolated from effluent samples collected from the discharge and drainage pipes of three textile industries.• The textile effluent non-adapted bacteria were isolated from soil.• Isolations were done on nutrient agar using enrichment culture techniques and the organisms identified to the generic level
  • 11.  A stock dye solution is prepared by dissolving 5.6 g dyes mix/L.
  • 12.  In 20 ml simulated effluent, 2 x 10 cfu of the 5 isolate was added in transparent bottles After 14 days decolourization and COD removal were measured Decolourization was determined by measuring the absorbance of the simulated effluent on uv spectrophotometer.
  • 13.  The isolates were screened for plasmids using the plasmid isolation technique (kado and lin, 1981)by electrophoresis on 0.8% agarose gels. The gels were stained with ethidium bromide and viewed on a UV transilluminator.
  • 14. RESULTRESULTS
  • 15. Source and identity ofisolates• A total of 24 isolates were obtained.• eighteen organisms isolated from the textile effluents• Bacillus,• Acinetobacter,• Legionella,• Staphylococcus and• Pseudomonas
  • 16.  six isolates from the soil sample. Bacillus spp.
  • 17.  The majority of the effluent adapted isolated showed colour-removing activities between 40.74 and 47.73% where as non-adapted isolates show 40.25 to 46.63. while the COD activities are between 17.91 and 36.69% of adapted isolates and non adapted show high COD.
  • 18.  Plasmids were not detected in any of the isolates from the effluent adapted or non- adapted isolates.
  • 19. DISCUSSION
  • 20.  In textile industries, During the dyeing process a substantial amount of dyes and other chemicals are lost in the waste water. Dye losses at between 10–15% (Vaidya and Datye, 1982). In the environment, dyes color water bodies and may hinder light penetration thereby affecting aquatic life and limiting the utilization (Ajayi and Osibanjo, 1980; Goncalves et al., 2000).
  • 21.  It has been reported that a typical textile effluent contains a dye mass concentration of 10–50 mg/L (Clarke and Anliker, 1980). However, the human eye can detect levels as low as 0.005 mg/L of reactive dyes in a clear river (Pierce, 1994).
  • 22.  In our study a sample with a dye mass of 5.6 g/L was used. This study discovered effluent adapted strains of Acinetobacter, Bacillus and Legionella with potentials for color removal and Strains of Acinetobacter, Bacillus and Pseudomonas with potential use for COD removal
  • 23. very few strains can withstand the conditions ofdyeing effluents(Maeir et al., 2004)
  • 24.  we have discovered textile effluent adapted strains of Acinetobacter and Bacillus, and effluent non-adapted Bacillus species with potential use in effluent treatment.
  • 25. Questions ?