1. CURRICULUM VITAEPersonal notesName Marco BerzanoDate and place of birth 09.07.1975 Turin (Italy)Address100 Moyglare Village, Maynooth, Co. Kildare - IrelandSpoken languages Italian (mother tongue)/English (advanced level)Education1989-1994 Attend LiceoScientifico (High School), graduating with “Diploma di MaturitàScientifica” on July 1994 (mark: 42/60)1994-2002 Attend the University Course in Biological Sciences at the University of Camerino; Graduation on 19 December 2002 presenting a thesis titled: “Approccio alla costruzione di un Microarray molecolare per l’identificazione di Diatomee presenti nelle acque dolci” (Mark: 110 with laude).2001 Six months as ERASMUS exchange student in Northern Ireland (UK) at the University of Ulster -Coleraine- working with Prof. R.Marchant and Prof. Ibrahim Banat – Dip. School of Biological and Environmental Sciences. The main topic of the project concerned the study of hyperthermophilic bacteria in soil (for more details see the list of publications). In addition, three exams in clinical microbiology were successfully undertaken.2003-2006 May 2003, started the PhD in the laboratory of Prof. C.O.Gualerzi at the University of Camerino working on the EU Project “MICROPAD- “Microarrays for the detection of the adundance and distribution of Pathogenic Protozoa, Flagellated Algae and Diatoms”. On 08/09/2006 PhD dissertation and proclamation. PhD title: “MOLECULAR TOOLS AND FRESHWATER DIATOMS, A NOVEL APPROACH TO IDENTIFY TARGET SPECIES TO ASSESS WATER QUALITY IN AQUATIC HABITAT”.
2. Professional experiences1993-1994 Worked at the Cuneo Golf Club (Cuneo, Italy) as caddy and “starter” during official competitions.1995-2002 Tour leader for a travel agency (TR Viaggi, Treia –MC-, Italy). I have worked for a local travel agency leading groups throughout Europe2002 Worked six months as secretary of the EU project MICROPAD, planning the design of the website setting up graphics and databases, writing scientific reports and maintaining communication within the European partnership.2003 Practical work on microarrays concerning the EU project MICROPAD under the supervision of Dr G.Muyzer and Dr C.Meyer at the TU-Delft, Department of Microbiology, NL. Performed research at the AWI for Marine and Polar Research-Bremerhaven-Germany under the supervision of Dr L.K.Medlin.2004-2005 Senior tutor at the University of Macerata, eLearning Hons degree for prospective teachers in primary school. In charge of two courses (Animal Biology, Environmental Biology) and two laboratories (Laboratory of Animal Biology and laboratory of Environmental Biology).2005-2008 Senior tutor on-line at the University of Macerata, as mentioned for 2004. In charge of 9 courses (Animal Biology, Environmental Biology 1 and 2, Evolutionary Biology, Genaral and Human Biology, Biology of humans with elements of Genetics, Laboratory of Environmental Biology and Laboratory of Maths and Science). Scientific consultant for an industrial patent in collaboration with a small enterprise (ORIM MetalliS.p.a, Macerata - Italy).2006 Local organizer of the workshop “Detection of Microbial Biodiversity in Environmental Samples - A Molecular EcologyWorkshop”. University of Camerino, Italy, 19-21 September 20052006 Research assistant (8 months) at the University of Camerino (prof. C.O.Gualerzi) working on freshwater diatoms and water quality assessment using phylochip technology.200701/01/200731/12/2008 Marie Curie Post-Doctoral Position at the University Collage of Dublin, Ireland. Work is based at the Central Veterinary Research Laboratory, Dept. Agriculture, Fisheries and Food, Co. Kildare. This position supervised byProf. N.Holdenin relation to the FP6 EU funded project Cryptonet.ie. The
3. scientific objective of the project was to develop a Cryptosporidium specific Microbial Risk Assessment (MRA) model for catchment waters that could be used to identify when potable water was at high risk of being contaminated.2008 Reviewer for “Journal of Plankton Research”2009-2010’09 April – ’10 November Postdoctoral researcher at the Westminster University, London working with Prof. Jane Lewis on the EU funded project MIDTAL (FP7) which aims to develop a universal microarray chip for the identification of toxic algae (HABs).Microarrays development, validation of sampling protocols and RNA quality tests, reporting and workshop organizer.2009- ongoing Laboratory analyst at the Department of Agriculture, Food and the Marine, Bacteriology/Parasitology Division.Samples processing and development of novel molecular protocols for the analysis of microbial pathogens and animal parasites.Quality internal auditor for the National reference laboratories.Higher Educational training2001-2002 Final year project (to complete the Ms Degree in Biological Sciences) carried out at the Dip. of Biology MCA Lab. of Genetics under the supervision of Prof. C.O. Gualerzi and Dr R. Spurio a project concerning the development of nucleotide probes for the identification of diatoms as bioindicators.2002March Attended the “basic course in microarray data analysis and mining” held by Prof. RaffaeleCalogero, Univeristy of Turin, Torino, Italy.February Attended the practical Course of the ARB package, basic level, (www.arb-home.de) at MPI in Bremen for molecular probe design and phylogenetic studiesApril Attended Workshop “ASCMAP–Analysis of Single Cells in Marine Phytoplancton”: 15-21 April.June 27 – July 04 Attended the practical course “Bioinformatics: computer Methods in Molecular Biology” at the ICEGB in Trieste (http://www.icgeb.trieste.it/)2004
4. September- November Specialization course for On-line tutors, or E-tutors for distance learning environments at the University of Macerata. Final examination passed, qualified E-tutor.July Attended the practical Course of the ARB package, advanced level, (www.arb-home.de) at MPI in Bremen for molecular probe design and phylogenetic studiesSeptember Attended the practical course “Basics in Bioinformatics” at the ISS (IstitutoSuperiore di Sanità, Rome), focused on Phylogenetic tools, like Paup, and GCG.2005June EMBO practical course “Microarray Technology: From Production to Applications” in HeidelbergSeptember Local organizer of the Molecular Ecology Workshop: “Detection of Microbial Biodiversity in Environmental Samples”, Camerino (MC), Italy.2007 Advanced Training at the TATAA Biocentre (Prague, CZ) for real time qPCR [Core module (3 days) and Biostatistics (2 days)]2009 Diversity program and health&safety tests as new appointee at the University of Westminster.2010 Internal QMS Auditor TrainingTeaching and supervising experiences2003-2006 Supporting lecturer for the course of Dr A.Laterza (Molecular Ecology) and for Dr A.Concetti (Applied Biochemistry) respectively in the Ms course in Biotecnology and CTF (Pharmaceutical and Chemical Thechnologies) at the University of Camerino. Lectures covered different topics of Bioinformatics, including the ARB package software. Students had practical experience on informatics tools available for applied research in molecular biology.2003-2006 Supervisor of a technician and an undergraduate student at the Department of Biology MCA (lab. Genetics), University of Camerino. Main duties concerned training in Molecular Biology and Microbiology techniques besides the safety prcedure of working in a laboratory.2007-2008 Supervisor of 2 PhD students and transfer of knowledge for for the molecular biology techniques within the EU project Cryptonet.ie (CVRL –Dep. Agriculture, Fishery and Food- and UCD, Dublin). Co-supervisor of a PhD student at the University of Camerino (Mai Nguyien). Supervisor of Ms student working on the development of molecular tools for the detection of Anisakis spp. in fish2009 Different roles covered at the University of Westminster:
5. - Supporting staff for CELL SCIENCE MODULE: 3SBS474.Y. Main responsabilities include running tutorials, practicals and marking of working assays. Course developer of Cell Science module FBMS405 for distance learning students (blended course starting on September 2010). Supporting lecturer for module 3BLY614.Y MOLECULAR BIOLOGY & DISEASE DIAGNOSIS and MOLECULAR APPLICATIONS IN FORENSIC SCIENCE 3BLY619.2010 Supervisor of one MSc student in collaboration with the Dublin Institute of Technology.Additional skills(include techiniques and instruments used)I have always worked as part of a team and I strongly believe that unambiguous rolesmixed with a bit of enthusiasm represent the synergy that permits the achievement ofgood results other than a pleasant working environment. I am familiar with activelistening and problem solving techniques that have been helping me in workenvironment toward difficulties and relationships with colleagues and customers. WhileI am working I am always extremely precise and meticulous my good organizationalskills and experience I can be pragmatic when the circumstances require that such asstrict dead lines or difficult situations. I think of myself as an energetic person who hasa focus on a solutions approach to problems and a “can do” attitude. High level of knowledge on Macintosh and Windows platform, basic on Unix systems. Microsoft Office, Adobe Photoshop, database designing (Access). Software for bioinformatics analysis: DNAstar®, MrBayes, Paup, ARB package, GenEx (MultiID) and other software for sequence editing and phylogenetic studies. Good knowledge in bacterial microbiology and microbiology techniques, including isolation of new bacterial strains SEM and TEM microscopy techniques as well as Fluorescence microscopy PCR based techniques, both standard and in real time (qPCR, RT-PCR), Radioactive labelling techniques, DNA sequencing, Microarray technology, Molecular cloning, Slot/Dot-Blot analysis, RFLP. DNA sequencing and fragment typing Diatom culturing and isolation techniques Identification of pathogenic protozoa such as Cryptosporidumspp. and Giardia (molecular typing) Identification of parasite such as Anisakis spp., Alariaalatausing molecular tools Identification of common zoonotic parasite (Microscopy-based) Working experience under the ISO 17025 Experience in Quality Auditing within the National Reference Laboratory (CVRL)
6. List of publications 1. T.M. Murphy, J. O’Connell, M. Berzano, C. Dold, J.D. Keegan, A. McCann, D. Murphy, N.M. Holden The prevalence and distribution of Alariaalata, a potential zoonotic parasite, in foxes in Ireland (acceptedby Parasitology Research, December 2011) 2. De Waele, V., Berzano, M., Speybroeck, N., Berkvens, Mulcahy, G.M., Murphy, T.M.Peri-parturient rise of Cryptosporidium oocysts in cows: new insights provided by duplex quantitative real-time PCR. (accepted by Veterinary Parasitology, Jan 2012) 3. Stephen McDonald, Marco Berzano, Peter Ziegler, Thomas M. Murphy & Nicholas M. HoldenQualitative Risk Assessment of Surface Water Contamination with Cryptosporidium spp. Oocysts: A Case Study of Three Agricultural Catchments. Human and Ecological Risk Assessment: An International Journal,Volume 17, Issue 4, 2011 -pages 813- 825DOI:10.1080/10807039.2011.588148 4. De Waele V, Berzano M, Mulchy G and Murphy T M. Age-stratified Bayesian analysis to estimate sensitivity and specificity of four diagnostic tests detecting Cryptosporidium spp. oocysts in calves.J ClinMicrobiol. 2011 Jan;49(1):76-84. Epub 2010 Nov 3 5. Nguyen M, Berzano M, Gualerzi C.O, Spurio R, Development of molecular tools for the detection of freshwater diatomsJournal of Microbiological Methods. 2011 Jan;84(1):33-40. Epub 2010 Oct 16. 6. Murphy TM, Berzano M, OKeeffe SM, Cotter DM, McEvoy SE, Thomas KA, OMaoileidigh NP, Whelan KF.Anisakid larvae in Atlantic salmon (Salmosalar L) grilse and post smolts: molecular identification and histopathology.J Parasitology. 2009 Sep 13:1. 7. Metfies K, Berzano M, Mayer C, Roosken P, Gualerzi CO, Medlin L and Muyzer G.An optimized protocol for the identification of diatoms, flagellated algae and pathogenic protozoa with phylochipsMolecular Ecology Notes 7 (6) 2007, 925– 936. 8. Metfies K, Berzano M, Gualerzi C, Muyzer G and Medlin L,Meeting Report: Molecular Ecology Workshop. Detection of Microbial Biodiversity in Environmental Samples, Camerino, Italy, September 19–21, 2005Protist. 2006 Aug;157(3):247-50. Epub 2006 Jun 23 9. Marchant R, Banat IM, Rahman TJ, Berzano M. The frequency and characteristics of highly thermophilic bacteria in cool soil environments. Environ Microbiol. 4:595-602 (2002) 10.Marchant R, Banat IM, Rahman TJ, Berzano M. What are high-temperature bacteria doing in cold environments?Trends Microbiol. 10:120-1(2002)
7. Papers in preparation: 1. 2. Berzano M, McDonald S, Holden N, Murphy T M. Cryptosporidium parvum in wild deers, a reported case in Ireland.(Short communication in preparation) 3. McDonald S, Berzano M, Murphy T M, Holden N. Cryptosporidium in the background: analysis of in a river catchment showing no signs of clinical infection. (In preparation) 4. Berzano M, McDonald S, Ziegler P, Holden N, Murphy T M. Hands in slurry: comparative analysis to evaluate DNA extraction procedures for the identification of Cryptosporidium spp. (in preparation) 5. Berzano M, Marcheggiani S, Rombini S, Gualerzi CO, Spurio RThe application of oligonucleotide probes and microarrays for the identification of freshwater diatoms. (accepted:Hidrobiologia, special issue) 6. Joe Taylor, Marco Berzano, Linda Percy, Jane Lewis and Linda K. Medlin Preliminary results of testing of the MIDTAL microarray- chip to monitor toxic dinoflagellates in the Orkney Islands, UK (submitted on January 2012 to The Journal of Micropalaeontology)Grant applicationsSeptember 2001 Contribution to the proposal for the EU project MICROPAD, funded by the European Commission under the FP5 programme. The European Commission successfully funded the project (QLK3-CT-2002-01939, http://cordis.europa.eu/data/PROJ_FP5/ACTIONeqDndSESS IONeq112482005919ndDOCeq1743ndTBLeqEN_PROJ.htm)January 2008 Application submitted to the EPA-Ireland for a 3 years project called PATHOWASTE: Risk assessment of waste and drinking water plants for Cryptosporidium and Giardia species. Shortlisted but not funded for lack of fundings.April 2008 Application submitted to the EPA-Ireland for a 3 years fellowship to carry on a project titled: Monitoring environmental samples for zoonotic pathogens using microarray technology and qPCR. Evaluation: >70%; shortlisted but not funded for lack of fundings.January 2009 Contribution to the preparation of the FP7 project called “μAQUA. MicroArrays for Water Quality Assessment under Current and Future Climates”. The project involves 12 different partners from several EU countries. The project is funded and it started on February 2011. http://microaqua.eu/projectPoster and oral presentationsSince I was an undergraduate student I have presented oral and postercommunications at national and international conferences and I have experiences
8. presenting confidently my research in front of a large audience. Presentations are oftenappreciated for being clear and direct and my communicating skills are supported by asolid knowledge on computer graphics, in particular in the use of software such asPhotoshop® or Powerpoint.2003May 29-30 S.Benedetto (AP), Italy, annual meeting “Marine and environmental biochemistry, SIMBA2003”2004April Cortona 2004, Annual meeting of the Italian Genetic Society. Oral presentation: “La biodiversità delle diatomee quale possibile strumento per l’analisi della qualità delle acque”.September 18th International Diatom Symposium 2004, poster title: “The EU project micropad: microarrays for the detection of protozoa, algae and diatoms. Medlin, L.K., Metfies, K., Muyzer, G., Mayer, C., Berzano, M., Spurio, R. & Gualerzi, C.”30 September-3 October Riva del Garda, FISV (FederazioneItalianaScienzedella Vita), poster presentation: “Molecular tools for identification of diatoms in freshwaters: alternative strategies for the development of species-specific oligonucleotide probes”.2005April Oral presentation at the II Simposio sulle Tecnologie Avanzate: Applicazioni delle nanotecnologie per la difesa nei settori strutturale, elettronico, biotecnologico, “La tecnologia dei microarray applicata alle diatomee, un nuovo approccio per la valutazione qualitative delle acque superficiali” M.Berzano, S.Rombini, S.Marcheggiani, R.Spurio, C.O.GualerziAugust Oral presentation at the International Phycological Congress in Durban (South Africa) “Microarrays and freshwater diatoms, a novel approach to identify target species to assess water quality in aquatic habitats”.September Oral presentation at the Molecular Ecology Workshop: “Microarrays and freshwater diatoms, a novel approach to identify target species to assess water quality in aquatic habitats”, 19-21 September, Camerino (MC), Italy2007May 13-17 International Giardia and Cryptosporidium conference in Morelia (Mexico). Poster: Towards the development of an environmental risk assessment model for cryptosporidiosis.October 31 Invited speaker at the Worshop: Cryptosporidium and other protozoan enteric pathogens in the Irish Aquatic Environment– Sources, Reservoirs and Public Health Implications. Institute of Technology, Sligo -Ireland
9. Conferences2002July 21-26 Attended the EPC3 “Third European Phycological Congress” in Belfast2005August International Phycological Congress in Durban (South Africa). Invited speaker for an oral presentationSeptember Molecular Ecology Workshop held in Camerino, Italy (local organizer). Invited speaker, oral and poster presentation.2007May 13-17 II International Giardia and Cryptosporidium conference in Morelia (Mexico). Poster presentation.2009October 11-15 III International Giardia and Cryptosporidium conference (Orvieto). 3 Posters presentation and the oral talk “Hands in slurry: comparative analysis to evaluate DNA extraction procedures for the identification of Cryptosporidium spp. from agricultural slurry”.
10. References could be obtained from:- Prof. N.M. HoldenBioresources Research Centre / Biosystems EngineeringUCD School of Agriculture, Food Science and Veterinary Medicine- Agriculture and FoodScience Centre- University College Dublin- BelfieldDublin 4, IRELAND Tel: +353 1 7167460 Fax: +353 1 7167415 Email: email@example.com- Dr T. MurphyDepartment of Agriculture, Fishery and Food, Backweston Campus, CVRL(Parasitology), Stacumny Lane, Celbridge –Co. Kildare, Ireland Tel: +353 1 6157141 Email: firstname.lastname@example.org- Prof. C.O.Gualerzi (PhD Supervisor),Lab. Biologia Molecolare e Biotecnologie Microbiche, Dipartimento di Biologia M.C.A. –University of Camerino, Via Gentile III da Varano, 62032 CAMERINO (MC) - Italy telephone: work +390737403240 work fax +390737636216 email: email@example.com- Dr Gerard MuyzerProfessor in Microbial Systems Ecology, Dept. of Aquatic Microbiology (AMB)Institute for Biodiversity and Ecosystem Dynamics (IBED)University of Amsterdam (UvA)Post address: P.O. Box 942481090 GE Amsterdam ,The Netherlands Phone: +31-20-525-6948 Fax: +31-20-525-7832 E-mail: firstname.lastname@example.org Website: http://www.science.uva.nl/ibed/home.cfm- Prof. Linda K.MedlinObservatoireOcéanologique de Banyuls-sur-mer, LaboratoireArago,66651 Banyuls-sur-mer, FranceTelephone: Work +33-430192400 email: email@example.com home page http://www.awi.de/en/go/molecular_genetics