Simulated Field Trial of Mousticide against Aedes at UKM Campus

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  • 1. END OF PROJECT REPORT NN-001-2008Evaluation of Proprietary Formulations of Insect Repellent Containing EGX-101 and Testing Mosquito Larvicide products Based on Trypsin Modulating Oostatic Factor (TMOF) Against Dengue VectorsPROJECT LEADER: PROF DR SALLEHUDIN BIN SULAIMANPROJECT TEAM MEMBERS: PROF MADYA DR HIDAYATULFATHI OTHMAN NORASHIQIN BINTI MISNI MOHD SAIFUL AZLAN BIN NORDIN LAU MING SEONG SITI FATIMAH JOHARRY Project Duration: 24 (months) Project Start Date: November (month) 2008 (year) Project End Date: November (month) 2010 (year) Collaberations: 1
  • 2. OUTDOOR EVALUATION OF TMOF-BTI IN VARIOUS FORMULATIONS AGAINSTFIRST INSTAR OF Aedes aegypti Linnaeus IN THE AREA OF UKM CAMPUSBy Saiful Azlan Bin Nordin, Biomedical Sciences, FSKB, UKMSupervised by Prof. Dr. Sallehudin Sulaiman & PM. Dr. Hidayatulfathi Othman5th November 2010INTRODUCTION Combination of TMOF-Bti in rice husk and wettable powder formulations invarious weights were used in this trial in order to test the efficiency of thesecombinations against first instar Aedes aegypti larvae in outdoor area. The objective ofthis study was to evaluate the efficiency of TMOF-Bti formulations in various weights insimulated field conditions at UKM campus.METHODOLOGYSimulated Trial in the Area of UKM Campus Materials used in this trial were buckets and mosquito netting. Combination ofTMOF-Bti in rice husk formulation (2% TMOF + 2% Bti; 4% TMOF + 4% Bti) andwettable powder formulation were produced by EntogeneX Industries Sdn. Bhd. Thelarval food was crushed dry leaf powder where the leaves were collected from areasaround campus UKM. Aedes aegypti larvae used in this trial were reared in insectariumof the Faculty of Allied Health Sciences, UKM. 20 first instar Aedes aegypti larvae wereplaced in a plastic bucket containing 4 liters of water. TMOF-Bti combination in rice huskformulation with the following weights viz: 50 mg, 100 mg, 150 mg and 200 mgrespectively was each distributed in 4 liters of water in each bucket; while TMOF-Bti inwettable powder formulation were measured in the following weights viz: 2mg, 5 mg, 10mg and 20 mg respectively and each concentration was placed in each bucket.. Eachconcentration runs as duplicates. Crushed dry leaf powders were supplied as source offood for the larvae. All buckets were covered by mosquito netting. Larval mortality wasrecorded after 24 hours and weekly. A new batch of 20 1st instar larvae Aedes aegyptiwas introduced into the buckets weekly without additional TMOF-Bti formulationsadded.. 73
  • 3. RESULTSSimulated trial in the area of UKM campusSimulated trial had been carried out in the area of UKM campus. These trials werecarried out in different locations. The area where the study was carried out were atoutside of workers’ resting room in UKM, 2nd floor of Block C and 2nd floor of Block B inUKM.TMOF- Bti WETTABLE POWDER 20+20Figure 1 : Mean of Larval survival percentage after the exposure to TMOF-Btiin wettable powder formulation From 18th August 2010. 74
  • 4. TMOF- Bti RICE HUSK FORMULATION 2+2Figure 2 : Mean of Larval survival percentage after the exposure to TMOF-Btiin rice husk formulation in various weights (Rice Husk Formulation 2+2 )from 18th August 2010 75
  • 5. TMOF- Bti RICE HUSK FORMULATION 4+4Figure 2 : Mean of Larval survival percentage after the exposure to TMOF-Btiin rice husk formulation in various weights (Rice Husk Formulation 4+4).DISCUSSIONFrom the graphs of figure 1 until 3,we can see that there are similarity between all theseformulations, 0% of larval survival were recorded for the first two weeks of the studybeing carried out except for 10mg of rice husk formulation 4+4. This shows that theseformulations were very effective on the first 2 weeks of application for killing the 1stinstar Aedes aegypti larvae.From week 3 onward, the graph of larval survival shows increasing of larval survival.The percentage survival increased until the 5th week but still there were larvae killed by 76
  • 6. all the formulations and the maximum percentage for the larval survival was 50%.Thusthe three formulations of TMOF-Bti could retain their efficacy for 5 weeks. The reasonfor this to occur could probably due to the effect of TMOF giving the larvicidal effect inlong term duration. TMOF causes larvae to starve and thereby slow down the larvaldevelopment process, and finally lead to death. Bacillus thuringiensis israelensis (Bti) isshort-lived in field conditions (Zaritsky et al. 2008). The results showed the same trendas the trial continued for weeks 3, 4 and 5 respectively. Larval mortalities in control setsbasically were low from the first week till week 4. However, in week 5, there was asudden decreased in the numbers of larvae survived in the control buckets, mostprobably due to insufficient food. Changing of water environment in the buckets mightaffect the larval survivorship as well.CONCLUSIONTMOF-Bti in both rice husk and wettable powder formulations had proven to be effectiveagainst first instar larvae of Aedes aegypti. Thus,it could be another potential controlagent for dengue vectors. 77