Using Proteomics to Understand Crassostrea gigas’s Response to Multiple Stressors Emma Timmins-Schiffman (UW, SAFS) Brook Nunn (UW, Med. Chem.) David Goodlett (UW, Med. Chem.) Carolyn Friedman (UW, SAFS) Steven Roberts (UW, SAFS)
Ocean Acidiﬁcation • Current and projected changes in pCO2 are unprecedented Zeebe 2011
Ocean Acidiﬁcation and Bivalves pCO2 Energy/resource use Growth & calcification • Acidosis and shell dissolution • CO32- availability
Characterize how ocean acidification affects the oyster’sresponse to a second stressor
pCO2 and Mechanical Stress • pH Homeostasis • V-type proton ATPase subunit C1 (CGI_10004936)• Immune Response o Cathepsin L (CGI_10009231) o Heat shock protein 70 12B (CGI_10024293) o MAP kinase-activated protein kinase 2 (CGI_10003308)
Summary Proteomics can provide insight into the physiologicalresponse to ocean acidification.
Summary Exposure to multiple stressors can impact anorganism’s ability to mount a successful response toeither stressor.
Implications We should continue to consider multiple stressors when assessing responses to environmental change. 0.02 LowMS2 HighMS1 0.01 High1 High4 HighMS4 LowMS4 HighMS2 0.00 Axis 2 LowMS3 HighMS3 High2 High3 LowMS1 Low4 Low3 -0.01 Low2 Low1 -0.02 -0.02 -0.01 0.00 0.01 0.02 0.03 0.04 Axis 1
Acknowledgements • Taylor Shellfish: Joth Davis, Jason Ragan, Dustin Johnson• Friday Harbor Labs: Emily Carrington, Ken Sebens, Michael O’Donnell, Matt George, Michelle Herko• UW SAFS: Sam White, Mackenzie Gavery, Caroline Storer, Samantha Brombacker, Lisa Crosson, Julian Olden• UW Proteomics Resource: Priska von Haller, Jimmy Eng, Eva Jahan• Ronen Elad, Sam Garson, Chris Parrish• Funding: NOAA Saltonstall-Kennedy, RocketHub