Service forminternational

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  • 1. Service Core: Project Order Form and Sample Submission Instructions Page 1 of 3 Service Core Sample Submission Form [International] starting from total RNA or DNA Contact and Billing Information Project Contact: Name Institution Department / Building / Room / Floor Street Address City / State / Zip Province or Country Phone Number Fax Number E-mail address Billing Information: Quote Reference Number [Provided by SABiosciences] # P_ _ _ _ Purchase Order Number [Supplied by International Distributor] Project Information Array Catalog Number: Identify the array(s) to be used by Catalog Number and Name For example: “PAHS-011” Array Name: ie: “Human Inflammatory Cytokines & Receptors PCR Array” Identify control sample(s): Briefly describe the data analysis to be conducted including any special needs or requests. SABiosciences Corporation 888.503.3187 301.682.9200 support@SABiosciences.com www.SABiosciences.com
  • 2. Service Core: Project Order Form and Sample Submission Instructions Page 3 of 3 General Requirements and Sample Submission Guidelines: Total RNA RNA Amount: SABiosciences recommends that five micrograms (5 μg) of RNA be sent for each array analysis to be performed. If submitting 5 μg is not feasible in your project, contact a SABiosciences technical support representative. Note: SABiosciences recommends that 10 micrograms (10 μg) of RNA be sent for miRNA analysis. Recommended Isolation Methods: Cells: Extract and Purify with Qiagen RNeasy Kit (with on-column DNase treatment step, Qiagen Cat. No. 74104) Tissues: Extract with TRIzol® from Invitrogen (Catalog Number 15596-026), followed by purification of RNA using Qiagen RNeasy Kit with DNase treatment. Note: DNase treatment is an essential step for the PCR Array experiment. Notify SABiosciences if sample extraction has not included a DNase treatment step (extra charges may apply). Dissolve in RNase-free water (or 10 mM Tris, pH 8.0) at a concentration greater than 0.5 mg/ml. Do not use DEPC treated water. Report actual concentration and A260/A280 and A260/A230 ratios. (RNA samples requiring concentration or precipitation may require extra service charges). Confirmation of RNA Sample Integrity Provide picture of ethidium-bromide stained agarose gel or analysis report from Agilent 2100 BioAnalyzer. Demonstrate sharp, intact 28S and 18S rRNA bands or peaks. Please note that an RNA analysis surcharge may apply if RNA if of too poor quality to proceed with hybridization. Check that your quotation includes all of the arrays and samples for your analysis project. The use of multiple array products may require separate orders or extra charges. Shipment: International Customers: Ship samples on a Monday or a Friday so that they arrive at SABiosciences within Monday Friday. Ship samples on at least 25 pounds (lbs) of dry ice in a suitable container (use at least enough dry ice to guarantee that the samples arrive frozen – be sure that you fulfill the shipping regulations for dry ice with your courier). Include an invoice/ packing list which describes the contents as follows: – – – – – – – RNA or DNA Samples for Research Use Only Reagents for Laboratory Research Use Only Non-Dangerous, Non-Infectious No Commercial Value, Value for Customs Only Assigned value for customs [ie. $1.00 US or 1.00 €] Number of samples and volumes Type of container Provide SABiosciences with tracking information to help ensure that they arrive safely and without delays. Email your technical support representative or support@SABiosciences.com and provide your quote number, courier name, and tracking number. Ship the samples with a completed Service Core Sample Submission Form including your RNA integrity analysis results and a copy of your purchase order and quote to: SABiosciences Attn: Service Core 6951 Executive Way Frederick, MD 21703 USA Phone: 1.301.682.9200 Priority for array service projects is assigned on a first-come, first-serve basis. Return shipment of leftover RNA samples following analysis can be arranged for an additional shipping fee. Contact SABiosciences to set up return shipment within one week of arrival of your samples. SABiosciences will keep RNA extractions under frozen conditions for a maximum of 6 weeks following array analysis. SABiosciences Corporation 888.503.3187 301.682.9200 support@SABiosciences.com www.SABiosciences.com
  • 3. Service Core: Project Order Form and Sample Submission Instructions Page 2 of 3 Project Outline RNA Sample Information RNA Source (Species) RNA Source (Tissue / Cell Line) RNA Type (total, poly A+, aRNA) For all RNA samples, attach a picture of the RNA integrity analysis by either agarose gel or Agilent 2100 BioAnalyzer. # RNA Sample Identification Concentration (should match information on tube) ( μg / μl ) RNA Quality A260/A280 A260/A230 Amount Provided ( μg ) 1 2 3 4 5 6 7 8 9 10 11 12 For additional samples, print an extra copy of this page. Attach copy of RNA analysis results here (Additional fees may apply if RNAs are provided without quality analysis results)