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I psc and stem cell 2013

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  • 1. Reprogramming, Screening and Validation of iPSCs and Terminally Differentiated Cells using the qBiomarker PCR Array System Sample & Assay Technologies
  • 2. Outline of Webinar What are induced pluripotent Stem Cells (iPS Cells or iPSCs)? Methods for creating iPSCs Screening and Validation iPSCs using qPCR The qBiomarker PCR Array System Application Data Screening of iPSC for Pluripotency Summary, Questions and Answers -2- Sample & Assay Technologies
  • 3. Why iPSCs? What can they be used for? iPSCs are derived from somatic cells and are not subjected to moral/policy issues that surround embryonic stem cells. Not necessary to destroy or create embryos Not subjected to Federal/State/Political funding issues Uses of iPSCs in Biomedical Research: Basic Research into pluripotency and differentiation Applied Research into Disease Specific model systems Translational Research Uses in regenerative medicine -3- Sample & Assay Technologies
  • 4. What are inducible pluripotent Stem Cells (iPS or iPSCs?) Most common technique for iPSCs Creation Reprogram a somatic cell by the over expression of key regulatory factors: Yamanaka Factors: Oct4, Sox2, KLF4, c-MYC Thomson Factors: Oct4, Sox2, Nanog, Lin28 -4- Sample & Assay Technologies
  • 5. Basic Workflow in creating iPSCs -5- Sample & Assay Technologies
  • 6. Basic Workflow in creating iPSCs -6- Sample & Assay Technologies
  • 7. Basic Workflow in creating iPSCs Cell types: Fibroblasts Keratinocytes pancreatic beta cells Hepatocytes Any somatic cell? -7- Sample & Assay Technologies
  • 8. Basic Workflow in creating iPSCs Factors: Sox 2, Oct4, KLF4, c-Myc, Lin 28, Nanog siRNA, small molecules, ??? Cell types: Fibrobalsts Pancreatic beta cells Hepatocytes Any somatic cell? -8- Sample & Assay Technologies
  • 9. Basic Workflow in creating iPSCs Factors: Sox 2, Oct4, KLF4, c-Myc, Lin 28, Nanog siRNA, small molecules, ??? Reprogramming (2-3 weeks) Cell types: Fibrobalsts Pancreatic beta cells Hepatocytes Any somatic cell? -9- Sample & Assay Technologies
  • 10. Basic Workflow in creating iPSCs Factors: Sox 2, Oct4, KLF4, c-Myc, Lin 28, Nanog siRNA, small molecules, ??? Reprogramming (2-3 weeks) Cell types: Fibrobalsts Pancreatic beta cells Hepatocytes Any somatic cell? - 10 - Sample & Assay Technologies
  • 11. Basic Workflow in creating iPSCs Factors: Sox 2, Oct4, KLF4, c-Myc, Lin 28, Nanog siRNA, small molecules, ??? Delivery Methods: Infection (lentivirus) Transfection Electroporation Reprogramming (2-3 weeks) Cell types: Fibrobalsts Pancreatic beta cells Hepatocytes Any somatic cell? How do we validate Pluripotency? - 11 - Sample & Assay Technologies
  • 12. Pluripotency Validation How Can we validate the pluripotency of our iPSCs? Chimeria Formation Teratoma Formation Biochemical Assays ? ? ? Differentiation and Morphology (Microscopy) Biomarkers using qPCR - 12 - Sample & Assay Technologies
  • 13. Pluripotency Validation How Can we validate the pluripotency of our iPSCs? Chimeria Formation Teratoma Formation Biochemical Assays ? ? ? Differentiation and Morphology (Microscopy) Biomarkers using qPCR - 13 - Sample & Assay Technologies
  • 14. Using qPCR As a Screening and Validation Technology qPCR: (Real-Time PCR) Able to accurately identify and provide quantitation for nucleic acids Small amounts of sample needed Can look at multiple genes simultaneously SABiosciences’ Solution to qPCR is the PCR Array Collection of Wet-Bench Validated SYBR Green qPCR Assays Biological Content for understanding biological pathway or defining mechanism of action Standardized for any qPCR instrument Data Analysis and Biological Interpretation qBiomarker PCR Arrays use laboratory validated samples to define both a predictive Biomarker set of genes and data analysis algorithm to clearly differentiate between a Biological process or define disease. - 14 - Sample & Assay Technologies
  • 15. Key Considerations in qPCR Assay Design Cycling Conditions Perfect qPCR Assay Design Algorithm Mastermix Functional Validation (Wet-Bench Testing) Ensures all parameters are optimized - 15 - Sample & Assay Technologies
  • 16. Key Considerations in qPCR Assay Design Cycling Conditions Perfect qPCR Assay Design Algorithm Mastermix Functional Validation (Wet-Bench Testing) Ensures all parameters are optimized - 16 - Perfect qPCR Assay Re-validate Assay with New MM Sample & Assay Technologies
  • 17. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 17 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 18. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 18 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 19. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 19 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 20. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 20 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 21. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 21 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 22. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 22 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 23. qBiomarker PCR Arrays qBiomarker iPSC Colony Screening qBiomarker Screening PCR Arrays Samples 1 2 3 4 5 6 7 8 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL OCT4 ZFP42 GAPDH GDC RTC PPC 8 samples per Plate - 23 - 8 predictive Biomarkers 1 Normalization Gene GDC (Genomic DNA Control) RTC (Reverse Transcription Control) PPC (Positive PCR Control) Sample & Assay Technologies
  • 24. qBiomarker Screening and Validation PCR Arrays 2 different formats depending on number of Biomarkers qBiomarker Screening PCR Arrays 8 samples/plate iPSC Colony Screening Yamanaka Factors Thomson Factors qBiomarker Validation PCR Arrays 4 samples/plate iPSC Pluripotency Validation - 24 - Sample & Assay Technologies
  • 25. qBiomarker PCR Arrays Biological Content Biomarkers are selected using multi-step process 1. Text mining and literature research 2. Large gene list developed 3. Biological Samples assayed through collaboration 4. Predicative Gene Signature based on Results 5. Companion Data Analysis Algorithm trained and tested - 25 - Sample & Assay Technologies
  • 26. How do qBiomarker PCR Arrays Work? Isolate Total RNA from Your Sample Isolate RNA using QIAGEN RNeasy . cDNA Synthesis (C-03 kit) – – . Genomic DNA Removal Step (5 min.) Reverse Transcription Step (20 min.) Load Plates Multiple Samples per PCR Array 2 minutes with multi-channel pipet . Run 40 cycle qPCR Program Standard cycling conditions for all Real Time PCR Instruments 2 hours . Upload and Analyze Data (FREE) 10 minutes from Raw Ct values to interpretation of Biology and Fold Change results - 26 - Sample & Assay Technologies
  • 27. How do qBiomarker PCR Arrays Work? Isolate Total RNA from Your Sample Isolate RNA using QIAGEN RNeasy . cDNA Synthesis (C-03 kit) – – . Genomic DNA Removal Step (5 min.) Reverse Transcription Step (20 min.) Load Plates Multiple Samples per PCR Array 2 minutes with multi-channel pipet . Run 40 cycle qPCR Program Standard cycling conditions for all Real Time PCR Instruments 2 hours . Upload and Analyze Data (FREE) 10 minutes from Raw Ct values to interpretation of Biology and Fold Change results - 27 - Sample & Assay Technologies
  • 28. How do qBiomarker PCR Arrays Work? Isolate Total RNA from Your Sample Isolate RNA using QIAGEN RNeasy . cDNA Synthesis (C-03 kit) – – . Genomic DNA Removal Step (5 min.) Reverse Transcription Step (20 min.) Load Plates Multiple Samples per PCR Array 2 minutes with multi-channel pipet . Run 40 cycle qPCR Program Standard cycling conditions for all Real Time PCR Instruments 2 hours . Upload and Analyze Data (FREE) 10 minutes from Raw Ct values to interpretation of Biology and Fold Change results - 28 - Sample & Assay Technologies
  • 29. How do qBiomarker PCR Arrays Work? Isolate Total RNA from Your Sample Isolate RNA using QIAGEN RNeasy . cDNA Synthesis (C-03 kit) – – . Genomic DNA Removal Step (5 min.) Reverse Transcription Step (20 min.) Load Plates Multiple Samples per PCR Array 2 minutes with multi-channel pipet . Run 40 cycle qPCR Program Standard cycling conditions for all Real Time PCR Instruments 2 hours . Upload and Analyze Data (FREE) 10 minutes from Raw Ct values to interpretation of Biology and Fold Change results - 29 - Sample & Assay Technologies
  • 30. How do qBiomarker PCR Arrays Work? Isolate Total RNA from Your Sample Isolate RNA using QIAGEN RNeasy . cDNA Synthesis (C-03 kit) – – . Genomic DNA Removal Step (5 min.) Reverse Transcription Step (20 min.) Load Plates Multiple Samples per PCR Array 2 minutes with multi-channel pipet . Run 40 cycle qPCR Program Standard cycling conditions for all Real Time PCR Instruments 2 hours . Upload and Analyze Data (FREE) 10 minutes from Raw Ct values to interpretation of Biology and Fold Change results - 30 - Sample & Assay Technologies
  • 31. Melting Curve Analysis Single Dissociation Peaks for Every Gene Assay • Example of QC criteria for every PCR Assay manufactured by SABiosciences • Single peak dissociation curves • Single gel bands of predicted size - 31 - Sample & Assay Technologies
  • 32. Melting Curve Analysis Single Dissociation Peaks for Every Gene Assay • Example of QC criteria for every PCR Assay manufactured by SABiosciences • Single peak dissociation curves • Single gel bands of predicted size - 32 - Sample & Assay Technologies
  • 33. Melting Curve Analysis Single Dissociation Peaks for Every Gene Assay • Example of QC criteria for every PCR Assay manufactured by SABiosciences • Single peak dissociation curves • Single gel bands of predicted size - 33 - Sample & Assay Technologies
  • 34. Compatible Instrumentation: 96- & 384-Well Formats 96-Well Blocks: 7000, 7300, 7500, 7700 FAST 96-Well Blocks: 7500, 7900HT FAST 384-Well Block: 7900HT StepOnePlus iCycler, MyiQ, MyiQ2, iQ5, CFX96, CFX384 Opticon, Opticon 2, Chromo 4 Mastercycler ep realplex 2/2S/4/4S LightCycler 480 TP-800 Mx3000p, Mx3005p, Mx4000p qBiomarker PCR Arrays are guaranteed when using qBiomarker qPCR SYBR Green MasterMix - 34 - Sample & Assay Technologies
  • 35. Data Analysis is Simple, Quick and Predictive An integral part of ALL qBiomarker PCR Arrays Each Pathway has a specific Data Analysis Template Interprets Gene Expression Changes into Biological Process . Are my iPSCs Pluripotent? What is the expression level of endogenous reprogramming Factors? Exogenous? Have my iPSCs undergone spontaneous differentiation? Are my cardiomyocytes or neurons terminally differentiated? - 35 - Sample & Assay Technologies
  • 36. Screening iPS Cells for Pluripotency Experiment: Created 6 different iPS cell lines from fibroblasts following 3 weeks of reprogramming. Which cell lines are totally pluripotent and worth using? iPS Colony Screening Delta Ct Values are interpreted - 36 - Sample & Assay Technologies
  • 37. Screening iPS Cells for Pluripotency Experiment: Created 6 different iPS cell lines from fibroblasts following 3 weeks of reprogramming. Which cell lines are totally pluripotent and worth using? iPS Colony Screening Delta Ct Values are interpreted - 37 - Sample & Assay Technologies
  • 38. Screening iPS Cells for Pluripotency Experiment: Created 6 different iPS cell lines from fibroblasts following 3 weeks of reprogramming. Which cell lines are totally pluripotent and worth using? iPS Colony Screening Delta Ct Values are interpreted Drop iPSC 1 and iPSC 2 from further experiments. - 38 - Sample & Assay Technologies
  • 39. Example of Graphs ∆ Ct Value Norm alized by GAPDH ∆ Ct Value Norm alized by GAPDH 18 18 16 16 Fib 14 DNM T3B 12 iPSC 1 12 DPPA4 iPSC 2 10 iPSC 3 iPSC 4 8 ∆ Ct ∆ Ct 14 GDF3 10 LEFTY1 NANOG 8 iPSC 5 6 iPSC 6 4 ESC PODXL POU5F1 4 2 6 ZFP42 2 0 0 DNM T3B DPPA4 GDF3 LEFTY1 NANOG PODXL POU5F1 ZFP42 Fib iPSC 1 iPSC 2 iPSC 3 Genes iPSC 5 iPSC 6 ESC Sam ples Fold Change Comparing to Control Sample Fold Change Comparing to Control Sample 5.0 5.0 4.5 4.5 4.0 iPSC 1 3.5 iPSC 2 3.0 iPSC 3 2.5 iPSC 4 2.0 iPSC 5 1.5 iPSC 6 1.0 ESC F o ld ch an g e (L o g 10) F old ch ang e (L o g10) iPSC 4 0.5 DNMT3B 4.0 DPPA4 3.5 GDF3 3.0 LEFTY1 2.5 NANOG 2.0 PODXL 1.5 POU5F1 1.0 ZFP42 0.5 0.0 DNMT3B DPPA4 GDF3 LEFTY1 NANOG PODXL POU5F1 0.0 ZFP42 iPSC 1 Genes iPSC 2 iPSC 3 iPSC 4 iPSC 5 iPSC 6 ESC Samples - 39 - Sample & Assay Technologies
  • 40. Application Data Determining The Pluripotent Status of iPSCs: . One of the problems facing iPSC researchers is spontaneous differentiation and loss of pluripotency during culture and passage conditions. Are my IPSCs still pluripotent? ESC A.1 iPSC #1 iPSC #2 IMR-90 iPSC Valdation PCR Array - 40 - Sample & Assay Technologies
  • 41. iPSC Pluripotency Validation - 41 - Sample & Assay Technologies
  • 42. iPSC Pluripotency Validation - 42 - Sample & Assay Technologies
  • 43. Summary Reprogramming qBiomarker Expression Lentivirus qBiomarker Expression Plasmids - 43 - Sample & Assay Technologies
  • 44. Summary Reprogramming qBiomarker Expression Lentivirus qBiomarker Expression Plasmids iPS Cell Induction •iPSC colony screening •iPSC Pluripotency Validation •Yamanaka Factors •Thomson Factors •Reprogramming Factors Expression - 44 - Sample & Assay Technologies
  • 45. Summary Reprogramming qBiomarker Expression Lentivirus qBiomarker Expression Plasmids iPS Cell Induction •iPSC colony screening •iPSC Pluripotency Validation •Yamanaka Factors •Thomson Factors •Reprogramming Factors Expression iPS Cell Differentiation •Embryoid Body •Neuronal Differentiation •Cardiomyocytes Differentiation - 45 - Sample & Assay Technologies
  • 46. Summary qBiomarker PCR Arrays Laboratory validated performance and biological content Available for any qPCR instrument Companion Data Analysis for interpretation of results Applications: Screening or Validation of iPS Cell inductions Validation of Cardiomyocyte and Neuronal Differentiation Complete System for Reprogramming, Screening and Validating iPS Cells - 46 - Sample & Assay Technologies
  • 47. Thank you for attending our Webinar! Questions, Comments or Suggestions? Please type all questions into the “Questions” Box. - 47 - Sample & Assay Technologies