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Gel electrophoresis power point

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Transcript

  • 1. Ellie Landis
  • 2. What is Gel Electrophoresis? Gel electrophoresis is a process that separates fragments of DNA based on their sizes.
  • 3. Step 1 Place DNA into tubes DNA can come from tissue orbody fluid, such ascheek cells, blood, skin, and hair.
  • 4. Step 2 Polymerase Chain Reaction The polymerase chain reaction uses a machine called a thermocycler to quickly copy a piece of DNA. Continue these three steps 30 to 40 times to get lots of copies of DNA.
  • 5. Step 3 Place restriction enzymes into DNA The restriction enzymes cut the DNA into different sizes according to it’s sequence.
  • 6. Step 4 Dye DNA and place into gel The gel is made with The gel is made out of wells at one end so agarose, which is similar that the DNA can be to jello. loaded into the gel.
  • 7. Step 5 Run electric current through gel DNA is negativelycharged so it will move towards the positivelycharged end of the gel.
  • 8. Step 6 Smaller pieces of DNA travel farther than Larger pieces of DNA End result!
  • 9. Gel Electrophoresis is used to... Solve criminal cases Solve paternity cases Diagnose genetic diseases Determine genetic kinship among species
  • 10. Pros Cons Easy to do  Expensive DNA does not get ruined in the  Time consuming process  Uses hazardous material You only need a small amount of DNA to start with DNA can be detected no matter what size it is
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