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METABOLISM    OFAMINO ACIDS
Lecture 11.     Introduction2.     Amino acid classification3.     Some definitions:     - nitrogen balance (NB)     - pro...
*Metabolism of proteins is the metabolism of amino acids.      NH2          COOH*Metabolism of amino acids is a part of th...
 Essential amino acids : Lysine, Leucine, Isoleucine, Valine, Methionine, Phenylalanine, Threonine, Tryptophan  Nonessen...
Nitrogen Balance (NB):Nitrogen balance is a comparison between Nitrogen intake (in the form of dietary protein)          ...
Three states are known for NB:a)Normal adult: will be in nitrogen equilibrium,               Losses = Intakeb)Positive Nit...
Protein Requirement for humans in       Healthy and Disease Conditions     The normal daily requirement of protein for    ...
Biological Value for Protein (BV):* BV is : a measure for the ability of dietary  protein to provide the essential amino a...
DIGESTION OF PROTEIN• Proteins are broken down by hydrolyases (peptidases or  proteases):• Endopeptidases attack internal ...
I. Gastric Phase of Protein Digestion:         (represents 15% of protein digestion)  1) Pepsin: in adult stomach , secret...
II. Pancreatic Phase of Protein Digestion• This phase ends with free amino acids and small peptides of 2-8 amino  acid res...
III. Intestinal Phase of protein digestion:– Intestinal enzymes are:  aminopeptidases (attack peptide bond  next to amino ...
Absorption of Amino Acids and      Di- &Tripeptides:
Absorption of Amino Acids and           Di- &Tripeptides:*L-amino acids are actively transported across the intestinal muc...
The transcellular movement of amino acids in an                 intestinal cells:                                         ...
Tri- & Dipeptides can actively transported faster than             their individual amino acids.intact proteins:        ...
METABOLIC FATES OF AMINO             ACIDS:1- Body protein biosynthesis.2- Small peptide biosynthesis(GSH).3-Synthesis of ...
Sources & fatesof amino acids:                                               Dietary protein•Protein turnover :           ...
Metabolism OF AMINO ACIDS:                                           R1. Removal of amonia by :      NH2         CH     CO...
Deamination of Amino Acids                             -NH3       Amino acid                         -Keto acid a) Oxidat...
2) Amino Acid Oxidases: The minor pathway for deamination of amino acids. They are found in peroxisomes of liver and kidne...
•D-amino acid oxidases are highly activethan L-amino acid oxidases especially inkidney and liver due to:    the function o...
b) Non-oxidative deamination:               (Direct Deamination )1) Deamination by dehydration:    Serine & Threonine     ...
2) Deamination by desulfhydration :                   (cysteine) CH2SH                            CH2           CH3       ...
Transamination:     NH2            O                                  O            NH2                                    ...
The role of PLP as Co-aminotransferase : PLP binds to the enzyme via schiff’s base & ionic salt bridge & helps in transfer...
Metabolic Significance of Transamination                   Reactions It is an exchange of amino nitrogen between   the mo...
Transdeamination:Amino acidAmino acid                          -ketoacid                                                 ...
THE FATE OF CARBON-SKELETONS OF                 AMINO ACIDSa) Simple degradation:(amino acid                  Common metab...
The common metabolic intermediates that arised from thedegradations of amino acids are: acetyl CoA, pyruvate, one of the k...
Metabolism of the Common Intermediates1.Oxidation: all amino acids can be oxidized in  TCA cycle with energy production2.F...
METABOLISM OF AMMONIAAmmonia is formed in body from:a) From amino acids: 1.Transdeamination in liver (NOT T.A.)           ...
Metabolic Disposal of AmmoniaAmmonia is toxic to CNS, it is fixed into nontoxic metabolite for reuse orexcretion according...
..                  H2O                              GlutaminaseGlutamine                                                 ...
c) Urea Formation Urea is the principal end-product of protein   metabolism in humans. It is important route for detoxic...
The Urea                          oCycle                          H2(The        NADOrnithineCycle,KrebsHenseleitCycle):   ...
Metabolic Significant Aspects of Urea CycleA) Energy Cost:. Energy cost of the cycle is only one ATP.B) urea cycle is rela...
Importance of Urea Cycle1. Formation        of   arginine  (in     organisms   synthesizing arginine) & formation of urea ...
Regulation of Urea Cycle1) Activity of individual enzymes:THE RATE LIMITING STEPS a) carbamoyl phosphate synthase-1       ...
2) Regulation of the flux through the cycle:a) Flux of ammonia:     1.by amino acids release from muscle (alanine,        ...
ONE-CARBON FRAGMENT METABOLISM• Human body is unable to synthesize the methyl group and obtain it from diet.• The first: m...
10   NH                            +    NADP+                  NH                         NADP  N           CH2 NADPH + H ...
• These one-carbon units are interconvertible to each other.• The primary sources of one-carbon units are serine, glycine,...
METABOLISM OF INDIVIDUAL AMINO                            ACIDS1. Metabolism of Glycine: nonessential, glucogenic.    Bios...
3            +    (CH3)3 -N-CH2 COO- + Homocysteine                       Met + (CH3)2 -N -CH2COO-     Betaine            ...
Special Functions of Glycine: a-Protein, Hormones & enzymes. b- Heme      c- Purines (C4,C5,N7)              d- Creatine e...
2. Formation of creatine (Methyl guanidoacetate)                          kidney                                          ...
CREATININE                                                          NON ENZYMATIC                                         ...
CO2 + NH3                      N5,N10                      CH2THF            serine               BetaineProtein          ...
2.     Metabolism of Serine: nonessential & glucogenic• It is synthesize from glycine or• intermediate of glycolysis,• all...
Degradative Pathways of Serine:1.           Serine              Glycine     CO2+NH3 (major)2.               Ser. dehydrata...
3 phospho-                 glycerate                              Phospho-     glycine                   protein          ...
3.     Metabolism of Sulfur-Containing amino acids       (Methionine, cyteine & Cystine):a)    Metabolism of methionine: (...
In transmethylation there are:     Methyl acceptors            Methyl Compounds1- Guanidoacetic acid                 Creat...
Transmethylation        Pi + PPi                                                                  S-adenosylmethionine    ...
b)     Metabolism of Cysteine& Cystine:       - They are interconvertable &They are not essential       - can be synthesiz...
Degredative pathway of cysteine:            Transamination               (Transamination)                                 ...
Biochemical functions of cysteine1- PAPS Formation: (3-phosphoadenosine,5-phosphosulphate)active   sulphate used in format...
Methionine           homocysteinecystine                   Taurine             cysteinepyruvate                  PAPS     ...
Polyamines (Spermidine & Spermine) :(1) Spermine & spermidine are growth factors, so they are   important in cell prolifer...
Biosynthesis:                                                                  ArginineMet.    SAM                        ...
Catabolism of Polyamine                             H2O 2         Polyamine oxidaseSpermine oxidase                       ...
4.          Aromatic amino acids            a) Metabolism of Phenylalanine                                         (glucog...
b) Tyrosine is a precursor of:1.DOPA (3,4 dihydroxy phenylalanine)                                                   NH2  ...
2.Thyroid hormones:  Thyroxine Formation:               I                NH2                                I             ...
Thyroglobulin(Tgb)• It is the precursor of T3 and T4• It is large, iodinated, glycosylated protein.• It contains 115 tyros...
Biosynthesis of Thyroid hormonesIncludes the following steps:1. Concentration of iodide:    the uptake of I by the thyroid...
c) Tryptophan (essential,glucogenic&ketogenic)I] 3-hydroxyanthranilic acid pathway:                                       ...
II] Serotonin Pathway:                                  NH2                               CH2CHCOOH                       ...
III] Melatonin formation pathway It is the hormone of pineal body in brain of man.  Formed by the acetylation and methyla...
IV] Indol, skatol and indicant pathway: •   Indol & skatol contributes to unpleasant odour of feces. •   Skatoxyl and indo...
Aromatic                                                  Amino                                                   Acids   ...
5.     Basic Amino Acids:       1) Histidine (glucogenic amino acid):a) Together with B-alanine , It forms carnosine (B-al...
(2) Arginine: (nonessential & glucogenic amino acid): It participates in formation of:     a)Creatine     b)Polyamines    ...
3) Lysine: (essential, ketogenic)it is involved in the formation of histone, hydroxylysine &carnitine:                    ...
6.    Acidic Amino Acids :      1.Glutamic acid : (nonessential & glucogenic amino acid).It participates in formation of:1...
2. Aspartic acid: Acidic, non essential & glucogenic 1. Arginosuccinate in urea cycle. 2. Alanine by decarboxylation. 3. O...
14 metabolism of amino acids
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Transcript of "14 metabolism of amino acids"

  1. 1. METABOLISM OFAMINO ACIDS
  2. 2. Lecture 11. Introduction2. Amino acid classification3. Some definitions: - nitrogen balance (NB) - protein requirement - biological value (BV)4. Digestion of protein5. Absorption of protein6. Metabolic fate of protein7. Metabolism of amino acids: - removal of ammonia: by deamination, transamination and transdeamination - fate of carbon skeletons of amino acid - metabolism of ammonia
  3. 3. *Metabolism of proteins is the metabolism of amino acids. NH2 COOH*Metabolism of amino acids is a part of the nitrogen metabolism in body.*Nitrogen enters the body in dietary protein.*Dietary proteins cannot be stored as such but used for formation of tissue proteins due to there is a continuous breakdown of endogenous tissue proteins.
  4. 4.  Essential amino acids : Lysine, Leucine, Isoleucine, Valine, Methionine, Phenylalanine, Threonine, Tryptophan  Nonessential amino acids: Alanine, glycine, aspartate , glutamate, serine, tyrosine, cysteineN.B.proline , glutamine, aspargine , N.B. Histidine & arginine are semi essential. They are essential only for infants growth, but not for old children or adults where in adults histidine requirement is obtained by intestinal flora & arginine by urea cycle.  For formation of new tissue protein : all essential amino acids that can not be synthesized by organism & provided by dietary protein must be present at the same time with nonessential amino acids that can be synthesized by organism
  5. 5. Nitrogen Balance (NB):Nitrogen balance is a comparison between Nitrogen intake (in the form of dietary protein) and Nitrogen loss (as undigested protein in feces , NPN as urea, ammonia, creatinine & uric acid in urine, sweat & saliva & losses by hair, nail, skin).NB is important in defining 1.overall protein metabolism of an individual 2.nutritional nitrogen requirement.
  6. 6. Three states are known for NB:a)Normal adult: will be in nitrogen equilibrium, Losses = Intakeb)Positive Nitrogen balance: Nitrogen intake more than losses (High formation of tissue proteins) occurs in growing children, pregnancy, lactation and convulascence.C)Negative Nitrogen balance: Nitrogen losses more than intake occurs in:- (Low intake of proteins) in starvation, malnutrition, GIT diseases - (High loss of tissue proteins ) in wasting diseases like burns, hemorrhage& kidney diseases with albuminurea - (High breakdown of tissue proteins ) in D.M., Hyperthyroidism, fever, infection
  7. 7. Protein Requirement for humans in Healthy and Disease Conditions The normal daily requirement of protein for adults is 0.8 g/Kg body wt. day-1.• That requirement is increased in healthy conditions: during the periods of rapid growth, pregnancy, lactation and adolescence.• Protein requirement is increased in disease states: illness, major trauma and surgery.• RDA for protein should be reduced in: hepatic failure and renal failure
  8. 8. Biological Value for Protein (BV):* BV is : a measure for the ability of dietary protein to provide the essential amino acids required for tissue protein maintenance.* Proteins of animal sources (meat, milk, eggs) have high BV because they contain all the essential amino acids.* Proteins from plant sources (wheat, corn, beans) have low BV thus combination of more than one plant protein is required (a vegetarian diet) to increase its BV.
  9. 9. DIGESTION OF PROTEIN• Proteins are broken down by hydrolyases (peptidases or proteases):• Endopeptidases attack internal bonds and liberate large peptide fragments (pepsin, trypsin, Chymotrypsin & Elastase)• Exopeptidases remove one amino acid at a time from – COOH or –NH2 terminus (aminopeptidase & carboxypeptidase)• Endopeptidases are important for initial breakdown of long polypeptides into smaller ones which then attacked by exopeptidases.• Digestion of protein can be divided into: a gastric, pancreatic and intestinal phases.
  10. 10. I. Gastric Phase of Protein Digestion: (represents 15% of protein digestion) 1) Pepsin: in adult stomach , secreted as pepsinogen.It is specific for peptide bond formed by aromatic or acidic amino acids HCL Pepsinogen Pepsinoligopeptides & polypeptides + amino acid Protein 2) Rennin: in infants for digestion of milk protein (casein).
  11. 11. II. Pancreatic Phase of Protein Digestion• This phase ends with free amino acids and small peptides of 2-8 amino acid residues which account for 60% of protein digestion Small UNCHARGED ALIPHATIC BASIC intestine BASIC Dietary protein Trypsin Chymotrypsin Elastase Enteropeptidase Trypsinogen Chymotrypsinogen Proelastase
  12. 12. III. Intestinal Phase of protein digestion:– Intestinal enzymes are: aminopeptidases (attack peptide bond next to amino terminal of polypeptide) & dipeptidases– The end product is free amino acids dipeptides & tripeptides.
  13. 13. Absorption of Amino Acids and Di- &Tripeptides:
  14. 14. Absorption of Amino Acids and Di- &Tripeptides:*L-amino acids are actively transported across the intestinal mucosa (need carrier, Na + pump, Na+ ions, ATP). Different carrier transport systems are: a) For neutral amino acids. b ) For basic amino acids and cysteine. c) For imino acids and glycine. d) For acidic amino acids. e) For B-amino acids (B-alanine & taurine).*D-isomers transported by simple diffusion.
  15. 15. The transcellular movement of amino acids in an intestinal cells: LumenAmino acid Na+ (Symport) Cytosol Amino acid Na+ K+ (Antiport) Na+ K+ Amino acid blood Extracellular fluid
  16. 16. Tri- & Dipeptides can actively transported faster than their individual amino acids.intact proteins: 1. Immunoglobulins of colostrum areabsorbed by neonatal intestines through endocytosis without loss of their biological activity and thus provide passive immunity to the infants. 2. Vaccines (undigested polypeptides) in children and adults are absorbed without loss oftheir biological activity producing antigenic reaction and immunologic response.
  17. 17. METABOLIC FATES OF AMINO ACIDS:1- Body protein biosynthesis.2- Small peptide biosynthesis(GSH).3-Synthesis of non-protein nitrogenous (NPN) compounds (creatine, urea, ammonia and uric acid)4- Deamination & Transamination to synthesized a new amino acid or glucose or ketone bodies or produce energy in starvation.
  18. 18. Sources & fatesof amino acids: Dietary protein•Protein turnover : Synthesis non-(results from Body protein essential a.as. 400 g per day breakdownsimultaneous synthesis& breakdown ofproteins molecules)•Total amount of protein Body protein 400 g per day,synthesisin body of healthy adultis constant (due to rate Ketone bodiesof protein synthesis is GL.&Glycogen Fatty acid& steroidsequal to the rate of its CO2& Ebreakdown).
  19. 19. Metabolism OF AMINO ACIDS: R1. Removal of amonia by : NH2 CH COOH - Deamination Oxidative deamination 1) glutamate dehydrogenase in mitochondria 2) amino acid oxidase in peroxisomes Direct deamination (nonoxidative) 1) dea. by dehydration (-H2O) 2) dea. by desulhydration (-H2S) - Transamination (GPT & GOT) - and transdeamination.2. Fate of carbon-skeletons of amino acids3. Metabolism of ammonia
  20. 20. Deamination of Amino Acids -NH3 Amino acid -Keto acid a) Oxidative Deamination: 1) Glutamate dehydrogenase , mitochondrial , potent, major deaminase NAD NADH + H+ or NADP NADPH + H+Glutamat -ketoglutarate + NH3 H2 O Glu. dehydrogenaseIt is allosterically stimulated by ADP & inhibited by ATP, GTP & NADH.Thus, high ADP (low caloric intake) increases protein degradation high ATP ( well fed-state) decreases deamination of amino acids & increases protein synthesis.
  21. 21. 2) Amino Acid Oxidases: The minor pathway for deamination of amino acids. They are found in peroxisomes of liver and kidney. L-amino acid oxidases utilize FMN while D-a.a. oxidases utilize FAD. R R R a.a. Oxidase H2O H-C-NH2 C = NH C=O NH3 COOH FMN FMNH2 COOH COOH imino acid ketoacid O2 CatalaseH2O + O2 H2O 2
  22. 22. •D-amino acid oxidases are highly activethan L-amino acid oxidases especially inkidney and liver due to: the function of D-amino acid oxidases isthe rapid and irreversible breakdown of D-amino acids since:• D- amino acids are potent inhibitors toL-amino acids oxidases
  23. 23. b) Non-oxidative deamination: (Direct Deamination )1) Deamination by dehydration: Serine & Threonine CH2 CH3 CH2OH Ser dehydratase C = NH H-C-NH2 C-NH2 PLP COOH COOH COOH H2O Serine CH3 H2O HOOC - C = O Pyruvate NH3
  24. 24. 2) Deamination by desulfhydration : (cysteine) CH2SH CH2 CH3 Cys. desulfhydratase C-NH2 C = NHH-C-NH2 PLP COOH COOH COOH H2S Cysteine CH3 H2O HOOC - C = O Pyruvate NH3
  25. 25. Transamination: NH2 O O NH2 NH2 NH2 O Aminotransferase R1 - C - COOH + R2 C COOH R1 - C COOH + R2 C COOH PLP H H H  –KG GLU amino acid keto acid new keto acid new amino acidAminotransferases are active both in cytoplasm and mitochondria e.g.: 1. Aspartate aminotransferase (AST), Glutamate oxaloacetate transaminase (GOT), 2. Alanine aminotransferase (ALT), Glutamate pyruvate transaminase, (GPT) In all transamination reactions, -ketoglutarate ( –KG) acts as amino group acceptor. Most, but not all amino acids undergo transamination reaction with fewexceptions (lysine, threonine and imino acids)
  26. 26. The role of PLP as Co-aminotransferase : PLP binds to the enzyme via schiff’s base & ionic salt bridge & helps in transfer of amino group between amino acid and keto acid (KG): OO New keto acid(amino acid) R11 - CH - COOH R R1 1 - CH - COOH R R11 - C - COOH R NH2 N H2 O CH CH2NH2 CHO OH H2 O Enz OH Enz OH Enz CH3 H3C N N CH3 N Pyridoxamine O PLP-Enz R2 R2 - C - COOH H2 O (keto acid) NH2 NH 2 R2 - C - COOH R22 - CH - COOH R H2 O N New amino acid (new amino acid) CH 2 OH Enz CH3 N
  27. 27. Metabolic Significance of Transamination Reactions It is an exchange of amino nitrogen between the molecules without a net loss This metabolically important because:1) There is no mechanism for storage of a protein or amino acids.2) In case of low energy (caloric shortage), the organism depends on oxidation of the ketoacids derived from transamination of amino acids.3) It is important for formation of the non-essential amino acids
  28. 28. Transdeamination:Amino acidAmino acid -ketoacid Transamination Aminotransferase Transamination Funnel -ketoglutarate-ketoglutarate Glutamate Deamination with Glu.D.H. Deamination Glutamate dehydrogenase NH3 3 NH Due to…L-amino acid oxidases, but not glutamate dehydrogenase, can sluggish(decrease) the rate of deamination of the amino acids. So… the most important and rapid way to deamination of amino acids is firsttransamination with -ketoglutarate followed by deamination of glutamate. Therefore glutamate through transdeamination serves to a funnel ammonia from all amino acids.
  29. 29. THE FATE OF CARBON-SKELETONS OF AMINO ACIDSa) Simple degradation:(amino acid Common metabolic intermediate)Alanine PyruvateGlutamate -ketoglutarateAspartate Oxaloacetateb) Complex degradation:(amino acid--- Keto acid----- complex pathway---- Common metabolic intermediate) Amino acids whose ketoacids are metabolized via more complex pathway e.g. Tyrosine, Lysine, Tryptophanc) Conversion of one amino acid into another amino acid before degradation: Phenylalanine is converted to tyrosine prior to its further degradation.
  30. 30. The common metabolic intermediates that arised from thedegradations of amino acids are: acetyl CoA, pyruvate, one of the krebscycle intermediates (-ketoglutarate, succinyl CoA, fumarate& oxaloacetate) Citrate cycle
  31. 31. Metabolism of the Common Intermediates1.Oxidation: all amino acids can be oxidized in TCA cycle with energy production2.Fatty acids synthesis: some amino acids provide acetyl CoA e.g. leucine and lysine (ketogenic amino acids).3.Gluconeogenesis: ketoacids derived from amino acids are used for synthesis of glucose (is important in starvation). Glucogenic Ketogenic Glucogenic&Ketogenic Ala, Ser, Gly, Cys, Leu , Lys Phe,Tyr,Trp,Ile,Thr Arg, His, Pro, Glu, Gln, Val, Met, Asp, Asn.
  32. 32. METABOLISM OF AMMONIAAmmonia is formed in body from:a) From amino acids: 1.Transdeamination in liver (NOT T.A.) 2.amino acid oxidases and amino acid deaminases in liver and kidney.b) Deamination of physiological amines: by monoamine oxidase (histamine, adrenaline, dopamine and serotonine).c) Deamination of purine nucleotides: especially adenine nucleotides deaminase AMP IMP + NH3d) Pyrimidine catabolism.e) From bacterial action in the intestine on dietary protein & on urea in the gut. NH3 is also produced by glutaminase on glutamine .
  33. 33. Metabolic Disposal of AmmoniaAmmonia is toxic to CNS, it is fixed into nontoxic metabolite for reuse orexcretion according to the body needs:a) Formation of Glutamate: -KG + NH3 GDH T.A.  -Amino acid Glutamate Keto acidb) Glutamine Formation: Muscle, brain NH3 H2 O NH2 O NH2 Gln synthaseHOOC-CH2-CH2-CHCOOH H2N-C-CH2-CH2-CH-COOH Mg2+ Glutamate ATP ADP+Pi GlutamineGlutamine is storehouse of ammonia & transporter form of ammonia. In brain, glutamine is the major mechanism for removal of ammonia while in liver is urea formation...Circulating glutamine is removed by kidney, liver and intestine where it isdeamidated by glutaminase . c) Urea formation
  34. 34. .. H2O GlutaminaseGlutamine Glutamate + NH3 This reaction is important to kidney due to kidney excretes NH4+ ion to keep extracellular Na+ ion in body and to maintain the acid-base balance. Glu. Diet && Body Diet body -A . a. protein protein Purines,pyrimidines DeaminaseBiosynthesis Various nitrogen-Of purine & containing compounds Pyrimidine glutamine glutaminase GIT Kidney Bacterial Liver urease Urine Urine urea NH4+ Kidney
  35. 35. c) Urea Formation Urea is the principal end-product of protein metabolism in humans. It is important route for detoxication of NH3. It is operated in liver, released into blood and cleared by kidney. Urea is highly soluble, nontoxic and has a high nitrogen content (46%), so …it represents about 80- 90% of the nitrogen excreted in urine per day in man Biosynthesis of urea in man is an energy- requiring process. It takes place partially in mitochondria and partially in cytoplasm.
  36. 36. The Urea oCycle H2(The NADOrnithineCycle,KrebsHenseleitCycle): MDH NADH2 Glu Glu
  37. 37. Metabolic Significant Aspects of Urea CycleA) Energy Cost:. Energy cost of the cycle is only one ATP.B) urea cycle is related to TCA cycle: 1. CO2 2.Aspartate arises via transamination of oxaloacetate with glutamate. Thus, depletion of oxaloacetate will decrease urea formation (as in malonate poisoning). 3. Fumarate enters TCA cycleC) Sources of Nitrogen in urea :free NH3 and aspartate. N.B. glutamate is the immediate source of both NH3 (via oxidative deamination by Glu. Dehyd.) and aspartate nitrogen (through transamination of oxaloacetate by AST).
  38. 38. Importance of Urea Cycle1. Formation of arginine (in organisms synthesizing arginine) & formation of urea (in ureotelic organisms, man) due to presence of arginase.2. Liver shows much higher activity of arginase than brain or kidney for formation of urea while in brain or kidney is the synthesis of arginine.3. Synthesis of non-protein amino acids (ornithine and citrulline) in body.
  39. 39. Regulation of Urea Cycle1) Activity of individual enzymes:THE RATE LIMITING STEPS a) carbamoyl phosphate synthase-1 b) Ornithine transcarbamyolase. c) Arginase. N-acetylglutamate is activator for carbamoyl phosphate synthase-1 It enhances its affinity for ATP. It is synthesized from acetyl CoA and glutamate. its hepatic concentration increases after intake of a protein diet, leading to an increased rate of urea synthesis. Activity of ornithine transcarbamyolase is limited by the concentration of its co-substrate "ornithine".
  40. 40. 2) Regulation of the flux through the cycle:a) Flux of ammonia: 1.by amino acids release from muscle (alanine, glutamine), 2. metabolism of glutamine in the intestine 3. amino acids degradation in the liver. b) Availability of ornithine. c) Availability of aspartate: since aspartate is required in equimolar amounts with ammonia, this is satisfied by of transdeamination .3) Change in the level of Enzymes:• Arginase & other urea-forming enzymes are adaptive enzymes thus• a protein-rich diet will increase their biosynthesis rate & the opposite is true for low protein diet.• However, in starvation, where the body is forced to use its own tissue protein as fuel, there is an increase in urea-forming enzymes.
  41. 41. ONE-CARBON FRAGMENT METABOLISM• Human body is unable to synthesize the methyl group and obtain it from diet.• The first: met = S-adenosyl methionine (methyl donner = CH3 ) involved in transmethylation reaction• The second: tetrahydrofolic acid (FH4 or THF) which is a carrier of active one-carbon units (-CH3, -CH2, -CHO, -CHNH, -CH).
  42. 42. 10 NH + NADP+ NH NADP N CH2 NADPH + H NADPH + H+ 6C 5 9 N C CH2 8 7CH N DHF reductase DHF reductase CH2 10 N(Folic acid) NH H 5 H N CH2 CH (THF) CH2 N H (active form)The one-carbon group carried by THF is attached to its N5 or N10 orto both. Group Position on THF Methyl: -CH3 N5 Methylene -CH2 N5 , N10 Formyl -CHO N5 or N10 Formimino -CHNH N5 N5 , N10 Methenyl -CH
  43. 43. • These one-carbon units are interconvertible to each other.• The primary sources of one-carbon units are serine, glycine, histidine, tryptophan & betaine.• and their acceptors for biosynthesis a variety of biomolecules are Phosphatidylethanolamine, Guanidoacetic acid, nor-Epinephrine ,Thymine, Purine-C8 & Purine-C2 & homocysteine.
  44. 44. METABOLISM OF INDIVIDUAL AMINO ACIDS1. Metabolism of Glycine: nonessential, glucogenic. Biosynthesis of glycine: OH NH21 CH2-CH-COOH Hydroxymethyl transferase NH2-C H2-COOH PLP H2O Serine Glycine THF N5,N10CH 2THF (Mit.Enz)2 Glycine synthase complex. CO2 + NH3 + N5,N10CH2THF + Glycine + THF PLP NADH+ H+ NAD+
  45. 45. 3 + (CH3)3 -N-CH2 COO- + Homocysteine Met + (CH3)2 -N -CH2COO- Betaine Dimethylglycine THF-CHO THF H Fp oxidase H2N-CH2-COO- N-CH2CO O - THF-CHO Glycine Fp oxidase CH3 SarcosineHS-CH2-CH2-CH-COOH (Homocysteine) CH3-S-CH2CH2-CH-COOH (methionine) NH2 NH2 Degradative pathway: Hyperoxaluria 1. Reaction 2. 2. Amino acid oxidase H N2NCH2COO - CHO + NH3 COOH Glycine Fp glyoxylat (3) FpH2 O2 e TA COOH (2) (1) CO 2 COOH H2O 2 Oxalate HCHO Oxalate - KG + Glycine HCOOH
  46. 46. Special Functions of Glycine: a-Protein, Hormones & enzymes. b- Heme c- Purines (C4,C5,N7) d- Creatine e- Glutathione f- Conjugating reactions:  Glycine + Cholic acid  glycocholate.  Glycine + Benzoic acid  Hippuric acid1.Formation of Glutathione (GSH) Dest.FR & Peroxides -Glu - Cys synthaseGlutamate + Cysteine  - glutamyl Cysteine + Glycine ATP ADP + Pi ATP GSH synthase  -glutamyl cysteinyl glycine ADP+Pi (GSH)
  47. 47. 2. Formation of creatine (Methyl guanidoacetate) kidney Guanido Glycine liver acetate Arginine Nonenzymatic in muscle Creatinine Creatine phosphate
  48. 48. CREATININE NON ENZYMATIC Pi+H2O IN MUSCLE CPK • Creatine Creatine phosphate ATP ADP EXCESS ATP DURING EXERCISE• Cr-P is the storage form of high energy phosphate in muscle• Creatinine is excreted in urine & increases on kidney failure due to its filteration is decreased. Its level is constant per 24 hrs & is proportional to muscle mass in human.
  49. 49. CO2 + NH3 N5,N10 CH2THF serine BetaineProtein Oxalic acid synth glycinePorphyrin Conjugation (heme) reaction purine Creatine GSH
  50. 50. 2. Metabolism of Serine: nonessential & glucogenic• It is synthesize from glycine or• intermediate of glycolysis,• all enzymes are activated by testosterone in liver, kidney & prostate. CH2OP CH2OP Glu  KG CH2OP PLP C =o CHOH Dehydrogenase CHNH2 COOH O TA COOH COOH 3-Phosphoserine NAD NADH+H+3- phosphoglycerate 3- Phosphopyruvate NH2 NH2 Phosphatase HO CH2 - CH - COOH Serine Pi H2 O
  51. 51. Degradative Pathways of Serine:1. Serine Glycine CO2+NH3 (major)2. Ser. dehydratase Serine Pyruvate + NH3 PLP T.A. TCA Alanine CO 2 + H2O GlucoseSerine is important in synthesis of: a. Phosphoprotein b. Purines & pyrimidine c. Sphingosine d. Choline e. Cysteine
  52. 52. 3 phospho- glycerate Phospho- glycine protein Purines &pyruvate Serine pyrimidines cysteine sphingosine choline
  53. 53. 3. Metabolism of Sulfur-Containing amino acids (Methionine, cyteine & Cystine):a) Metabolism of methionine: (essential) Met. Cysteine (diet.pr.) 2 principal metabolic pathways: Transmethylation and transsulfuration Transmethylation COOH H2NCH CH2 ATP Pi + PPi NH CH2 NH2 CH3-SCH2CH2CH COOH + S - CH2 + Methionine O Adenine CH3 Methionine Adenosyl Transferase S-denosyl-methionine (SAM)
  54. 54. In transmethylation there are: Methyl acceptors Methyl Compounds1- Guanidoacetic acid Creatine2- Norepinephrine SAM Epinephrine3- Ethanolamine Choline4- Uracil Thymine SAH (S-Adenosyl Homocysteine)
  55. 55. Transmethylation Pi + PPi S-adenosylmethionine Me-acceptor SAM MethyltransferaseHomocystinuria ATP SAM synthase Me-product Lack of Methionine Methionine S-adenosyl HomocysteineCystathionine H4FA B12 Methyltransferase adenosine H2 O synthase N5CH3H4FA Homocysteine COOH H2N-CH COOH CHNH2 PLP CH2 + CH2 CH2 Cystathionine (Degradative pathway) synthase SH OH or Transsulfuration H2 O Serine COOH H2N-CH COOH COO COO CH2 CHNH2 CHNH CHNH CH2 - S CH2 2 2C-skeleton of cysteine CH + CH Cystathionase 2 2 Cystathionine SH CH PL H2 O From serine & Cysteine OH 2 Homoserine S from methionine NH3 deaminase PLP CoAS CO2 H -ketobutyrate propionyl CoA Succinyl CoA. NAD+ NADH+H+
  56. 56. b) Metabolism of Cysteine& Cystine: - They are interconvertable &They are not essential - can be synthesized from Met & Ser O2 + Fe2 + or Cu2+ SH S S (Oxidation) CH2 CH2 CH22 moles of CHNH2 H2NCH CHNH2 2 GSH COOH COOH COOH Cystine Cysteine Reductase NAD+ NADH+H+
  57. 57. Degredative pathway of cysteine: Transamination (Transamination) SH Oxidative pathway (Oxidative pathway) CH2 -KG Cys-dioxygenase SO2H H2NCH Fe++ , O2 TA CH2 GLu COOH NADPH NADP PLP CHNH2 Cysteine SH COOH H2 O 3-mercapto CH2 Non oxid. (non oxid. Cys-sulfinate C=O pathway) B6  KG pyruvate COOH pathway TA , PLP GLu H2S SO3-- SO 4-- SO2H Trans-sulfurase NH3 CH2 desulfinase C=O GSH COOHSO4-- MO2+, cyt b5 sulfite SO3-- H2 S Pyruvate Pyruvic acid B-sulfinyl pyruvate SO3-- SO4-- PAPS oxidase ATP PAPS (active SO4) ATP
  58. 58. Biochemical functions of cysteine1- PAPS Formation: (3-phosphoadenosine,5-phosphosulphate)active sulphate used in formation of sulfate esters of steroids, alcohol, phenol,some lipids, proteins and mucopolysaccharides2- Sulfur of COASH, GSH, vasopressin, insulin3-Detoxication reaction of bromo, chloro, iodobenzene, naphthalene and anthracene & of phenol, cresol, indol and skatol that is formed by the action of intestinal bacteria on some amino a cids in large intestine with formation of ethereal sulfates which is water soluble and rapidly removed by the kidney4- Taurine Formation ( with bile acids form taurocholate) SH SH SO 2H CO 2 SO 2H Cys-dioxygenas e CH2 2 CH CH2 CH2 e 2+ PLP H2NCH Fe , O 2 CH2 HN 2CH CHNH2 NADPH + H+ NADP+ COOH NH2 COOH COOH Cysteine Hypotaurine Cys-sulfinate SO 3H [O] NH2 - CH2 - CH2 (Taurine)
  59. 59. Methionine homocysteinecystine Taurine cysteinepyruvate PAPS GSH
  60. 60. Polyamines (Spermidine & Spermine) :(1) Spermine & spermidine are growth factors, so they are important in cell proliferation and growth.(2) They are important in stabilization of cells and subcellular organelles membranes.(3)They have multiple + Ve charges and associate with polyanions such as DNA, RNAs and have been involved in stimulation of RNA and DNA biosynthesis as well as their stabilization.(4)They exert diverse effects on protein synthesis and act as inhibitors of protein kinases
  61. 61. Biosynthesis: ArginineMet. SAM Ornithine PLP PLP CO 2 Decarboxylase CO 2 Decarboxylase H3N+ 2 4 1,4 Diaminobutane 1 3 NH3+ A Decarboxylated SAM Putrescine Spermidine Synthase A Methylthioadenosine H3N+ 2 1 3 H2N+ 1,3 Diaminopropane H3N+ Spermidine Decarboxylated SAM Spermine Synthase Methylthioadenosine 1,3 Diaminopropane H3N+ H2N+ H2N+ H3N+ Spermine
  62. 62. Catabolism of Polyamine H2O 2 Polyamine oxidaseSpermine oxidase Spermidine O2 B-aminopropionaldehyde Polyamine oxidase CO 2 + NH3 Putrescine O2 H2O 2
  63. 63. 4. Aromatic amino acids a) Metabolism of Phenylalanine (glucogenic & ketogenic) NH2 NH2 CH2-CH COOH O2 H2 O Phenylalanine hydroxylase CH2-CH COOH Phenylalanine H4 biopterine H2 biopterine OH Tyrosine α a KG NADP+ NADPH(H+) TA PLP Glu OH O O OH O CO 2 PHPP O2 CH2-C-COOH O Homogentisate OH monooxygenase C CH2COOHHC C-CH2COOH Oxidase 2+HC CH2 Fe Vit C, Cu2+ C O2 OH O OH OMaleylacetoacetate P-Hydroxyphenylpyruvate Homogentisate (PHPP) GSH isomerase Hydrolase Fumaryl acetoacetate Fumarate + Acetoacetate . H2 O glucose Ketone body
  64. 64. b) Tyrosine is a precursor of:1.DOPA (3,4 dihydroxy phenylalanine) NH2 NADP+ CH2CH COOH H4biopterin Tyrosinase (Cu++) NADPH(H+ Tyr-hydroxylase ) O 2 OH H biopterin 2 Tyrosine O2 DOPA O2 Tyrosinase NH2 H2O Cu++ CH2CH COOH Dopaquinone OH OH DOPA CO 2 Decarboxylase PLP CH2CH2NH2 Melanins in melanocytes. OH OH Dopamine Dopamine B-oxidase O2 VitC & OH OH CH3 Cu2+ CH CH2 NH2 CH CH2 NH SAM SAH Methyltransferase OH Norepinephrine OH OH OH Epinephrine
  65. 65. 2.Thyroid hormones: Thyroxine Formation: I NH2 I NH2 HO CH2CHCOOH HO CH2CHCOOH I 3-Monoiodotyrosine (MIT) 3,5 Diiodotyrosine (DIT) DIT I I NH2 I I NH2 HO O CH2CHCOOH HO O CH2CHCOOH I I I 3,5,3/ -Tri iodothyronine (T3) 3,5,3,5-Tetraiodothyronine (T4) I I NH2 HO O CH2CHCOOH I 3,3,5-Triiodothyronine (reverse T3)
  66. 66. Thyroglobulin(Tgb)• It is the precursor of T3 and T4• It is large, iodinated, glycosylated protein.• It contains 115 tyrosine residues each of which is a potential site of iodination.• 70% iodide in Tgb exists in the inactive forms MIT&DIT WHILE• 30% is in T3& T4• About 50 μg thyroid is secreted each day.
  67. 67. Biosynthesis of Thyroid hormonesIncludes the following steps:1. Concentration of iodide: the uptake of I by the thyroid gland is an energy dependent process & is linked to active Na pump.2. Oxidation of iodide: the thyroid is the only tissue that can oxidize I to a higher valence state3. Iodination of tyrosine: oxidized I reacts with tyrosine residues in thyroglobulin form MIT & DIT.4. Coupling of iodotyrosyls: The coupling of two DIT T4 or of MIT & DIT T3
  68. 68. c) Tryptophan (essential,glucogenic&ketogenic)I] 3-hydroxyanthranilic acid pathway: NH2 NH2 CH2CHCOOH CH2CHCOOH•Trp pyrrolase Trp pyrrolase C=O Inc.by N H O2 Fe++ N CHO Cortico. & Tryptophan H N-Formyl Kynurenine tryptophan NADPH(H+ ) NADP+ H2 O & Dec.by Kynurenine Formylase Niacin, Format e NH2 NAD & NADP NH2 H2 bioterin H4 biopterin H CH2CHCOOH CH2CHCOOH Kynurenine C=O C=O H2 O hydroxylase O 2 OH NH2 NH2 OH Kynurenine 3-Hydroxy kynurenine H2 O NH2 CH3CHCOOH Kynureninase PLP Alanine Nicotinamide nucleotide 3 steps (NAD & NADP) COOH 7 steps NH2 OH Acetoacetyl COA 3-Hydroxyanthranilic acid
  69. 69. II] Serotonin Pathway: NH2 CH2CHCOOH NH2 O2 Tryptophan H2 O hydroxylase HO CH2 CHCOOH N H N H4 bioterin H2 biopterin Tryptophan H 5-OH Tryptohpan decarboxylase PLP NADPH(H+) NADP+ CO 2* Neurotransmitter HO CH2 CH2 NH2 N H* Founds in mast cells& 5-OH Tryptamine (Serotonin) CH3COSCOA platelets. N-Acetyl Transferase COASH* Vasoconstrictor HO CH2CH2NHCOCH3 for B.V.& bronchioles N H N-acetyl 5-OH tryptamine* Transmitter in GIT to SAM O-methyl Transferase release the peptide SAH CH3O CH2CH2NHCOCH3 hormones. N H Melatonin (N-acetyl-5-methoxy-serotonin)
  70. 70. III] Melatonin formation pathway It is the hormone of pineal body in brain of man. Formed by the acetylation and methylation of serotonin. It has effects on hypothalamic-pituitary system. It blocks the action of MSH & ACTH. It is important in regulation of gonad & adrenal functions. It has a circadian rhythm due to its formation occurs only in dark,due to high activity of N-acetyl transferase enzyme so it is a biological clock. It keeps the integrity of cells during aging due to it has an antioxidant property It enhances the body defense against infection in AIDS patients by increasing the number of immune cells. It reduces the risk of cancer&heart diseases
  71. 71. IV] Indol, skatol and indicant pathway: • Indol & skatol contributes to unpleasant odour of feces. • Skatoxyl and indoxyl are absorbed from large intestine • and conjugated with sulfate in the liver • and excreted in urine as indican (K indoxyl sulfate). CO2 Bacteria in colonTryptophan indol acetic acid Skatol O2 CO2 O2 indoxyl indol Skatoxyl OSO 3K (indican) N H
  72. 72. Aromatic Amino Acids Phenylalanine Tyrosine Tryptophane Aceto Dopa & Skatol & fumarate Melanine Melatonin acetate Dopamine Indol Nor epinephringlucose ketone & Thyroxin Anthranilic Serotonin epinephrine Alanine Nicotinamide Acetoacetyl CoA
  73. 73. 5. Basic Amino Acids: 1) Histidine (glucogenic amino acid):a) Together with B-alanine , It forms carnosine (B-alanyl histidine) and anserine (methyl carnosine): 1. They are buffer the pH of anerobically contracting skeletal muscle 2.They activate myosin ATP-ase 3.They chelate copper and enhance Cu2+ uptake. b) Histidine is a source of one-carbon atom. c) decarboxylase Histidine HistamineHistamine is a chemical messenger that mediates allergic and inflammatory reactions, gastric acid secretion and neurotransmission in the brain.
  74. 74. (2) Arginine: (nonessential & glucogenic amino acid): It participates in formation of: a)Creatine b)Polyamines C)Nitric oxide NO (Free radical gas). NADPH(H+) NADP+ NO synthaseL-Arginine L-Citrulline O2 NO possesses tumoricidal and bactericidal action relaxes smooth muscle in macrophages. (vasodilation) prevents platelet aggregation neurotransmitter in brain
  75. 75. 3) Lysine: (essential, ketogenic)it is involved in the formation of histone, hydroxylysine &carnitine: CH3 H3C CH3 NH2 N+ (CH2)4 3 SAM 3 SAH (CH2)4 H-C-NH ...... protein H-C-NH ...... protein Transmethylation COOH COOH Lysine-bound protein Trimethyl lysine-bound protein CH3 Protease CH3 H3C CH3 H3C N+ N + CH3 Aldolase Hydroxylase CH3 H3C CH3 Oxidation (CH2)3 at B-carbon N+ (CH2)3 H2N-CH2-COOH CHOH (CH2)4 COOH glycine -Trimethyl ammonium butyrate H-C-NH2 H-C-NH2 Hydroxylase COOH COOH -OH Trimethyl Trimethyl lysine lysine CH3 H3C N + CH3  CH 2  CHOH  -Hydroxy--trimethylammonium butyrate  CH2 (carnitine) COOH
  76. 76. 6. Acidic Amino Acids : 1.Glutamic acid : (nonessential & glucogenic amino acid).It participates in formation of:1- GSH.2- Glutamine: as storage and transporter form of ammonia3- GABA (-aminobutyric acid) neurotransmitter in brain. PLP HOOC CH2 CH2 CH2 NH2 (GABA) HOOC CH2 - CH2 CH COOH Glu-decarboxylase Glutamic acid NH2 PLP TA NADH(H+) NAD+ HOOC CH2 - CH2 COOH HOOC CH2 CH2 CHO Succinic semialdchyde Succinic acid dehydrogenase Succinic semialdchyde
  77. 77. 2. Aspartic acid: Acidic, non essential & glucogenic 1. Arginosuccinate in urea cycle. 2. Alanine by decarboxylation. 3. Oxalate & glucose by T.A. Amino acids as precursors of neurotransmitters1. Arginine --------------NO2.Tryptophan-----------Serotonin3. Histidine--------------Histamine4. Phenyl alanine------dopa,dopamine, NE&E5.Glutamic acid--------GABA
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