IN-VITRO CELL BASED ASSAY- CORE ASSAY LIST 2011
CORE ASSAY LIST 2011THERAPEUTIC AREA – IMMUNOMODULATION                                                                   ...
THERAPEUTIC AREA – IMMUNOMODULATION                                                                                       ...
THERAPEUTIC AREA – IMMUNOMODULATION                                                                                       ...
THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION                                                                          ...
THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION                                                                          ...
THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION                                                                    ...
THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION                                                                    ...
THERAPEUTIC AREA – DIABETES/OBESITY                                                                                       ...
THERAPEUTIC AREA – ONCOLOGY                                                                                               ...
THERAPEUTIC AREA – IN VITRO GENOTOXICITY                                                                                  ...
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Core assay list 2011

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Core assay list 2011

  1. 1. IN-VITRO CELL BASED ASSAY- CORE ASSAY LIST 2011
  2. 2. CORE ASSAY LIST 2011THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of mitogens/inhibitors on cell proliferation in murine splenocytes/Human PBMC. Useful for lead Up to 3 compounds at CellCA01 optimization of proliferation/ anti-proliferation compounds. 4 Weeks 10 mg 5 concentrations tested Proliferation Assay format: Incubations in 48-well plates. MTT read out. in quadruplicate Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (un-stimulated) Nitric Oxide release from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA02 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. Colorimetric. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-2) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA03 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-3) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA04 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  3. 3. THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-4) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA05 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-5) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA06 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-6) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA07 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IL-10) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA08 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  4. 4. THERAPEUTIC AREA – IMMUNOMODULATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-12) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA09 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (IFN-γ) from murine splenocytes. Useful for lead Up to 3 compounds at Mediator optimization for pro-inflammatory compounds and potencyCA10 4 Weeks 10 mg 5 concentrations tested release determination. in quadruplicate Assay format: Incubations in 48-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  5. 5. THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) Nitric Oxide release from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA11 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. Colorimetric. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Prostaglandin release (PGE2) from murine macrophages. Useful Up to 4 compounds at Mediator for lead optimization for Anti-inflammatory compounds andCA12 4 Weeks 10 mg 5 concentrations tested release potency determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Thromboxane release (TXB2) from Human pro-myelocytic Up to 4 compounds at Mediator leukemia cells. Useful for lead optimization for Anti-inflammatoryCA13 4 Weeks 10 mg 5 concentrations tested release compounds and potency determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Leukotriene release (LTB4) from Human pro-myelocytic leukemia Up to 4 compounds at Mediator cells. Useful for lead optimization for Anti-inflammatoryCA14 4 Weeks 10 mg 5 concentrations tested release compounds and potency determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  6. 6. THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (stimulated) cytokine release (IL-1β) from murine macrophages. Useful for lead Up to 4 compounds at Mediator optimization for Anti-inflammatory compounds and potencyCA15 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Histamine release from rat basophilic leukemia cells. Useful for Up to 4 compounds at Mediator lead optimization for Anti-allergic compounds and potencyCA16 4 Weeks 10 mg 5 concentrations tested release determination. in triplicate Assay format: Incubations in 96-well plates. HTRF. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) cytokine release (MCP-1) from murine peritoneal macrophages. Up to 4 compounds at Mediator Useful for lead optimization for Anti-inflammatory compounds andCA17 4 Weeks 10 mg 5 concentrations tested release potency determination. in triplicate Assay format: Incubations in 96-well plates. ELISA. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  7. 7. THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Measurement of compound toxicity in hepatocytes against t-BH using viability (MTT) endpoints. Assess compound Up to 4 compounds at In vitro safety before proceeding to clinical development.CA18 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against diazinon (OPC) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA19 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against Aldrin (OC) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA19 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. Test model: Measurement of compound toxicity in hepatocytes against Aflatoxin (AFB1) using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA20 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  8. 8. THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Measurement of compound toxicity in hepatocytes against Galactosamine using viability (MTT) endpoints. Assess Up to 4 compounds at In vitro compound safety before proceeding to clinical development.CA21 4 Weeks 10 mg 5 concentrations tested Toxicity Assay format: Incubations in 96-well plates. In vitro toxicity in triplicate profiling. Data format: Word™ report with data available in an Excel™ file.THERAPEUTIC AREA – COSMETOLOGY Turn Minimum ProjectREF# Assay Principle Around Compound Standard Format Type Time Required Test model: Determine effect of compounds on (un-stimulated) collagen release from primary human dermal fibroblast. Useful for Up to 4 compounds atCA22 Anti-wrinkle lead optimization for cosmetology ingredient. 4 Weeks 10 mg 5 concentrations tested Assay format: Incubations in 96-well plates. Colorimetric. in triplicate Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (un-stimulated) Melanin release from mouse melanoma cells. Useful for lead Up to 4 compounds at SkinCA23 optimization for cosmetology ingredient. 4 Weeks 10 mg 5 concentrations tested whitening Assay format: Incubations in 96-well plates. Colorimetric. in triplicate Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  9. 9. THERAPEUTIC AREA – DIABETES/OBESITY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Measurement of induction of glucose stimulated Insulin secretion in rat insulinoma cells. This approach is useful for assessing the effectiveness of compounds under development for Up to 4 compounds atCA24 GSIS Assay the treatment of type 1 diabetes. 4 Weeks 10 mg 5 concentrations tested Assay Format: Incubations in 96-well plates. HTRF. in triplicate Data Format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Lipid Adipocyte differentiation in Mouse embryonic fibroblast . Useful Up to 4 compounds at MetabolismCA25 for lead optimization for inhibitors of adipogenesis. 4 Weeks 10 mg 5 concentrations tested Assays Assay format: Incubations in 96-well plates. Colorimetric. in triplicate (Adipogenesis) Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on (stimulated) Lipid glycerol release in Mouse embryonic fibroblast . Useful for loead Up to 4 compounds at MetabolismCA26 identification for inhibitors of adipolysis. 4 Weeks 10 mg 5 concentrations tested Assays Assay format: Incubations in 96-well plates. Colorimetric. in triplicate (Adipolysis) Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  10. 10. THERAPEUTIC AREA – ONCOLOGY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Determine effect of compounds on cell proliferation in human breast adenocarcinoma cells. Useful for lead optimization Anti Up to 4 compounds at of anti-cancer compound screening.CA27 proliferative 4 Weeks 10 mg 5 concentrations tested assay Assay Format: Incubations in 96-well plates. Colorimetric. in triplicate Data Format: WordTM report with data available in an ExcelTM file.THERAPEUTIC AREA – GENERAL HEALTH/ HEART DISEASE Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model: Determine the cellular antioxidant potential of compounds in Human hepatocellular liver carcinoma cells. Useful cost-effective method to evaluate the antioxidant activity of foods, Up to 4 compounds at AntioxidantCA28 dietary supplements, phytochemicals and extracts.. 4 Weeks 10 mg 5 concentrations tested assay Assay Format: Incubations in 96-well plates. Colorimetric. in triplicate Data Format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
  11. 11. THERAPEUTIC AREA – IN VITRO GENOTOXICITY Turn MinimumREF# Project Type Assay Principle Around Compound Standard Format Time Required Test Model:. Determine the mutageneic potential of compounds using 5 starins of S.Typhimurium with and without S9 fractions. Up to 1 compounds at Mutagenesis Quick assay to estimate the carcinogenic potential of a compoundCA29 4 Weeks 10 mg 6 concentrations tested assay Assay Format: Incubations in 384-well plates. Hightroughput. in duplicate. Data Format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on the formation of small membrane bound DNA fragments i.e. micronuclei in the cytoplasm of interphase cells using Chinese hamster cells. Useful Up to 1 compounds at MutagenesisCA30 for early identification of compounds likely to exhibit genotoxic 15 Weeks 10 mg 3 concentrations tested assay liability. in duplicate. Assay format: Incubations in 90mm petri plates. Manual. Data format: WordTM report with data available in an ExcelTM file. Test model: Determine effect of compounds on induction of structural changes in chromosomes using Chinese hamster cells. Up to 1 compounds at Cytogenetic Useful to identify agents that cause structural chromosomeCA31 15 Weeks 10 mg 3 concentrations tested Assays aberration in cultured mammalian cells. in duplicate. Assay format: Incubations in 90mm petri plates. Manual. Data format: WordTM report with data available in an ExcelTM file. R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: cvc@naturalremedy.com
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