Sydney Kushner Seminar

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Sydney Kushner Seminar

  1. 1. Eduardo E. RiveraDr. Sydney KushnerPolyadenylation helps Regulate FuntiontRNA Levels in Escherichia ColiSidney Kushner who came from the University of Georgia offered a seminar titled“Polyadenylation Helps Regulate Function tRNA Levels in Escherichia Coli.” The twoimportant terms in the seminar were polyadenylation and how do you makefunctional tRNA in Escherichia coli. He explained basic biological terms like thedefinition of polyadenylation which is the addition of a poly(A) tail to an RNAmolecule. tRNA are unusual because after they are made they are modified. Thereare around 30-35 modifications. One of the modifications was inosine, which I foundvery interesting. Inosine is actually adenasine denominated and is one of the post -transcriptional modifications made in tRNA. Using a wild type E. coli, they separatedfour tRNA into smaller units using enzymes. They were comparing what they saw inE. coli and in eukaryotes. When they saw that both E. coli and eukaryotes did notlook the same in the gel, they thought that if they eliminated enzymes, (which breakthe tRNA), it would change the results. In conclusion, they found that there is acompetition in the enzymes used to break the tRNA. On the other hand,polyadenalyation is effective in making and regulating a tRNA in E. Coli. Thesefindings can help to understand better the functional levels within the tRNAs.

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