Course 4997 Final Presentation

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  • Impotance: for plants as it is a source of nitrogen which forms the building blocks of molecules such as amino acids and DNA.
  • A form of hemoglobin that doesn’t carry oxygen through out the body called methemoglobin. N-nitroso compound found in the gastric juices on the stomach
  • Describiralrededores, como se cogieronlasmuestras…
  • Dimethylphenol method: TNT 835 – Sulfuric and phosphoric acid Solution A – isopropanol
  • Diferenciasignificativa p<0.001 t-test
  • Unted States Geological Survey Rain gauge:  gather and measure the amount of liquid precipitation over a set period of time.
  • Nitrates are more concentrated below or near the area of waste accumulation or disposal such as manure piles, feedlots, septic tank disposal fields, cesspools, privies, etc. Excess nitrates also are more apt to be found in ground water under low areas and waterways that collect or conveyFactors that maybe affected our results may have been the temperature because Nitrate levels generally are highest following wet periods and lowest, even down to zero nitrates, during dry periods which may cause a false sense of security.Nitrate levels may have decrease because If nitrate levels drop it can mean two things. One possibility is that the bacteria that break down either ammonia or nitrite have been killed off.
  • Course 4997 Final Presentation

    1. 1. Lysander Borrero Eduardo Rivera MBRS-RISE UPR at Cayey Dr. Javier Arce
    2. 2.  Background Information Objective Hypothesis Methodology Results Discussion Future Work References
    3. 3.  Nitrate:  Composition of one nitrogen atom and three oxygen atoms  Soluble in water at standard temperature and pressure http://www.chemistry.wustl.edu /~courses/genchem/Tutorials/Io http://sci.waikato.ac.nz/farm/images/a ns/images/nitrate3.jpg tomic%20structure%20of%20nitrate.p ng
    4. 4.  Nitrates are important for plants and algae to grow Fertilizers High levels of nitrate can cause:  Methemoglobinemia-Blue baby (Austin, 1999)  Cancer
    5. 5.  The nitrate levels will rise after a precipitation event in studied water bodies.
    6. 6.  Measure the levels of nitrates in water bodies after an extreme precipitation event.
    7. 7. Lake Sample River Sample
    8. 8.  Collect samples TNT 835 (Dimethylphenol Method)  Sample water (1 mL)  Solution A (200 µL) Spectrophotometer  Insert the vial  Reads the barcode  Measures nitrate levels
    9. 9. Results
    10. 10. Date Time Nitrate Concentration (mg/L)28/april/2012 9:00 am 0.07928/april/2012 4:45 pm 0.11129/april/2012 7:15 am 0.12229/april/2012 4:30 pm 0.11330/april/2012 9:30 am 0.14730/april/2012 6:38 pm 0.1371/may/2012 6:47 am 0.1371/may/2012 7:03 pm 0.4692/may/2012 9:00 am 0.0612/may/2012 6:48 pm 0.258
    11. 11. Date Time Nitrate Concentration (mg/L)28/april/2012 11:35 am 0.30328/april/2012 5:32 pm 0.57329/april/2012 11:45 am 0.21129/april/2012 6:01 pm 0.54030/april/2012 6:28 am 0.62830/april/2012 6:27 pm 0.7911/may/2012 6:37 am 0.7061/may/2012 7:12 pm 0.4702/may/2012 8:24 am 0.6002/may/2012 8:21 pm 0.730
    12. 12. Levels of nitrate in the river (blue bar) vs.levels of nitrate in the lake (pink bar)
    13. 13. http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=01_00065&format=img_default&site_no=50055225&set_arithscale_y=on&begin_date=20120425&end_date=20120502
    14. 14. Lake Sample  Before precipitation: from 4/28/2012am to 4/30/2012 am vs.  After precipitation: from 4/30/2012pm to 5/2/2012pm
    15. 15. River Sample  Before precipitation: from 4/28/2012 am to 4/30/2012 am vs.  After precipitation: from 4/30/2012 pm to 5/2/2012 pm
    16. 16. Lake Water River Water Concentration of nitrates  Concentration of nitrates 0.079-0.469 0.211-0.791 Nitrate level increased  Nitrates levels increased after precipitation event after precipitation event Nitrate level increased in  Nitrate level increased in 5/1/2012 pm 4/30/2012 pm
    17. 17.  Precipitation can be a factor in the levels of nitrates. Possible factors that affected our results:  Periods between samples  Storage of the samples  Agricultural and rural areas  Artificial lake
    18. 18.  Compare extreme dry periods vs. extreme precipitation event. Collect more samples for a longer period of time. Collect from the same water body, but on different sites.
    19. 19.  Dr. Javier Arce Ruby Otero RISE Program  Dr. EneidaDíaz  Dr. Elena González  Dr. Roberto Ross
    20. 20.  Hach Company. 2007. Nitrate. Revised 2010. Doc 316.53.01070. (p) 1-6 Kreitler C, Jones D. 1975. Natural Soil Nitrate: The Cause of Nitrate Contamination of Ground Water in Runnels County, Texas. 13 (1) Self JR, Waskom RM. July 1995. Nitrates in Drinking Water. Revised October 2008. Fact Sheet No. 0.517
    21. 21.  Austin A. 1999. Infantile Methehemoglobinemia: Reexamining the Role of Drinking Water Nitrates. Environmental Health Perspectives [Internet]; [Published 1999 June 1]. Volume(107):[approximately 4 p.]. Available on http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566680/? page=1
    22. 22.  United States Geological Survey  http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=01_00065 &format=img_default&site_no=50055225&set_arithscale _y=on&begin_date=20120425&end_date=20120502  http://waterdata.usgs.gov/pr/nwis/uv/?dd_cd=03_0004 5&format=img_default&site_no=50055225&set_arithscal e_y=on&begin_date=20120425&end_date=20120502

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