201310 springer mexico 120min

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201310 springer mexico 120min

  1. 1. Jeff  Robens,  PhD   Senior  Editor   Author  Academy:     Steps  to  Publica<on  Success   Entre  Pares   8  October  2013   edanzedi7ng.com/entrepares  
  2. 2. About Jeff… Author   Senior  Editor     Peer  reviewer   Cell  biology   Tissue  engineering   Neuroscience  
  3. 3. Why publish in English? ü  Interna<onal  language  of  academics   ü  Build  an  interna<onal  reputa<on     ü  Develop  interna<onal  collabora<ons   ü  Overseas  research  opportuni<es  
  4. 4. 1.   Journal  selec<on   2.   Manuscript  structure   3.  Targe<ng  the  journal   4.   Effec<ve  wri<ng   5.   Cover  leOers   6.   Peer  review   Today’s presentation …
  5. 5. Edanz survey results – Primary difficulty during manuscript preparation n  =  333  published  non-­‐na7ve  English  authors   Choosing  a   journal   Expressing  thoughts   clearly  in  English   Understanding  the   author  guidelines   FormaKng  according   to  the  guidelines   118   79   73   63   35.4%   23.7%   21.9%   18.9%  
  6. 6. Journal  selec3on   Section 1
  7. 7. Journal  selec7on   Timing Choose  your  target  journal:   – A#er  you  have  decided  you  have  enough   results  for  a  publica7on     – A#er  you  have  decided  on  how  high  to  aim— high,  medium  or  low  impact   – A#er  you  have  decided  how  broadly  relevant   your  findings  are   – Before  wri7ng  the  Title,  Abstract,  Introduc7on   or  Discussion  sec7ons  
  8. 8. Journal  selec7on   Tenga en cuenta los diferentes factores de cada revista Aims  &  scope   Readership   Open  access   Indexing   Which  factor  is  most  important  to  you?   Publica<on   frequency   Impact  factor   Varies  by  field  
  9. 9. Journal  selec7on   Choosing a target journal Honestamente  evalue  la  importancia     de  su  inves3gación     Significance   Aims  and  Scope   Impact  
  10. 10. Journal  selec7on   Evaluating significance How  new  are  your  findings?  Novelty   How  broadly  relevant  are  your  findings?  Relevance   What  are  the  important  real-­‐world   applica<ons?   Appeal  
  11. 11. Journal  selec7on   Insert  your  proposed  abstract   El  Journal  Selector  ayuda  a  elegir     la  publicacion  más  adecuada   Journal Selector – www.edanzediting.com/journal_selector
  12. 12. Journal  selec7on   Recommended  journals   Filter  by:   Impact  factor   Publishing  frequency   Open  access   Journal Selector – www.edanzediting.com/journal_selector
  13. 13. Journal  selec7on   Seman<c  matching  terms   Journals  IF,  Aims  &  Scope,     and  Frequency   Similar  published  ar<cles   Have  they  published  similar  ar<cles  recently?   Have  you  cited  some  of  these  ar<cles?   Journal Selector – www.edanzediting.com/journal_selector
  14. 14. Journal  selec7on   Visit journal websites
  15. 15. Journal  selec7on   Tips to identify the most suitable journal S   Iden<fy  the   interests  of  the   journal  editor   Iden<fy  the   interests  of  the   readers   •  Editorials   •  Review  ar<cles   •  Special  issues   •  Most  viewed   •  Most  cited   Iden3fica  los  intereses  del  editor  de  la  revista  y   de  los  lectores  en  cada  publicacion  
  16. 16. Manuscript  structure   Section 2
  17. 17. Coverage  and   Staffing  Plan   Manuscript   structure   IMRaD Ø  Title   Ø  Abstract   Ø  Introduc7on   Ø  Methods     Ø  Results   Ø  and   Ø  Discussion   The  beginning The  end The  middle
  18. 18. Coverage  and   Staffing  Plan   Manuscript   structure   Important  points   Summarize  key  finding   Contains  keywords   Less  than  20  words   Avoid   Ques<ons   Abbrevia<ons   “New”  or  “novel”   Effective titles El  Gtulo  debe  ser  conciso  y  resumir  su  hallazgos  clave  
  19. 19. Coverage  and   Staffing  Plan   Manuscript   structure   Abstract First  impression   of  your  paper   Importance  of   your  results   Validity  of  your   conclusions   Relevance  of     your  aims   Judge  your   wri<ng  style   Probably  only  part   that  will  be  read  
  20. 20. Coverage  and   Staffing  Plan   Manuscript   structure   Sections of an abstract Aims   Background   Methods   Results   Conclusion   Why  the  study  was  done  (20%)   Your  hypothesis  (10%)   Techniques  (10%)   Most  important  findings  (40%)   Conclusion/implica<ons  (20%)   Concise  summary  of  your  research  
  21. 21. Coverage  and   Staffing  Plan   Manuscript   structure   Unstructured abstract Our  understanding  of  the  mechanisms  by  which  ducts  and  lobules  develop  is  derived  from   model   organisms   and   three-­‐dimensional   (3D)   cell   culture   models   wherein   mammalian   epithelial  cells  undergo  morphogenesis  to  form  mul7cellular  spheres  with  a  hollow  central   lumen.  However,  the  mechanophysical  proper7es  associated  with  epithelial  morphogenesis   are  poorly  understood.  We  performed  mul7dimensional  live-­‐cell  imaging  analysis  to  track  the   morphogene7c   process   star7ng   from   a   single   cell   to   the   development   of   a   mul7cellular,   spherical   structure   composed   of   polarized   epithelial   cells   surrounding   a   hollow   lumen.   We   report  that  in  addi7on  to  ac7vely  maintaining  apicobasal  polarity,  the  structures  underwent   rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the  structures  rotated  360°  every  4  h  during   the  early  phase  of  morphogenesis.  Rota7onal  mo7on  was  independent  of  the  cell  cycle,  but   was  blocked  by  loss  of  the  epithelial  polarity  proteins  Scribble  or  Pard3,  or  by  inhibi7on  of   dynein-­‐based  microtubule  motors.  Interes7ngly,  none  of  the  structures  derived  from  human   cancer   underwent   rota7onal   mo7on.   We   found   a   direct   rela7onship   between   rota7onal   mo7on  and  assembly  of  endogenous  basement  membrane  matrix  around  the  3D  structures,   and  that  structures  that  failed  to  rotate  were  defec7ve  in  weaving  exogenous  laminin  matrix.   Dissolu7on   of   basement   membrane   around   mature,   nonrota7ng   acini   restored   rota7onal   movement   and   the   ability   to   assemble   exogenous   laminin.   Thus,   coordinated   rota7onal   movement  is  a  unique  mechanophysical  process  observed  during  normal  3D  morphogenesis   that  regulates  laminin  matrix  assembly  and  is  lost  in  cancer-­‐derived  epithelial  cells.   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  22. 22. Coverage  and   Staffing  Plan   Manuscript   structure   Unstructured abstract Conclusion  Thus,  coordinated  rota7onal  movement  is  a  unique  mechanophysical  process  observed  during  normal   3D  morphogenesis  that  regulates  laminin  matrix  assembly  and  is  lost  in  cancer-­‐derived  epithelial  cells.   Results   We   report   that   in   addi7on   to   ac7vely   maintaining   apicobasal   polarity,   the   structures   underwent   rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the  structures  rotated  360°  every  4  h  during  the  early   phase  of  morphogenesis.  Rota7onal  mo7on  was  independent  of  the  cell  cycle,  but  was  blocked  by  loss   of   the   epithelial   polarity   proteins   Scribble   or   Pard3,   or   by   inhibi7on   of   dynein-­‐based   microtubule   motors.  Interes7ngly,  none  of  the  structures  derived  from  human  cancer  underwent  rota7onal  mo7on.   We   found   a   direct   rela7onship   between   rota7onal   mo7on   and   assembly   of   endogenous   basement   membrane  matrix  around  the  3D  structures,  and  that  structures  that  failed  to  rotate  were  defec7ve  in   weaving   exogenous   laminin   matrix.   Dissolu7on   of   basement   membrane   around   mature,   nonrota7ng   acini  restored  rota7onal  movement  and  the  ability  to  assemble  exogenous  laminin.   Methods   We  performed  mul7dimensional  live-­‐cell  imaging  analysis  to  track  the  morphogene7c  process  star7ng   from   a   single   cell   to   the   development   of   a   mul7cellular,   spherical   structure   composed   of   polarized   epithelial  cells  surrounding  a  hollow  lumen.     Background   Our   understanding   of   the   mechanisms   by   which   ducts   and   lobules   develop   is   derived   from   model   organisms  and  three-­‐dimensional  (3D)  cell  culture  models  wherein  mammalian  epithelial  cells  undergo   morphogenesis   to   form   mul7cellular   spheres   with   a   hollow   central   lumen.   However,   the   mechanophysical  proper7es  associated  with  epithelial  morphogenesis  are  poorly  understood.     Wang  et  al.  PNAS.  2013;  110:  163‒168.   Escriba  su  reseña     en  secciones  
  23. 23. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract We   report   that   in   addi7on   to   ac7vely   maintaining   apicobasal   polarity,   the   structures   underwent  rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the  structures  rotated  360°   every  4  h  during  the  early  phase  of  morphogenesis.  Rota7onal  mo7on  was  independent   of  the  cell  cycle,  but  was  blocked  by  loss  of  the  epithelial  polarity  proteins  Scribble  or   Pard3,  or  by  inhibi7on  of  dynein-­‐based  microtubule  motors.  Interes7ngly,  none  of  the   structures  derived  from  human  cancer  underwent  rota7onal  mo7on.  We  found  a  direct   rela7onship   between   rota7onal   mo7on   and   assembly   of   endogenous   basement   membrane  matrix  around  the  3D  structures,  and  that  structures  that  failed  to  rotate   were   defec7ve   in   weaving   exogenous   laminin   matrix.   Dissolu7on   of   basement   membrane   around   mature,   nonrota7ng   acini   restored   rota7onal   movement   and   the   ability  to  assemble  exogenous  laminin.   Write  the  results  sec<on  first   ü  Key  findings  that  directly  support  your  aims   ü  Will  be  interes<ng  to  the  readers   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  24. 24. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract Our  understanding  of  the  mechanisms  by  which  ducts  and  lobules  develop  is  derived   from   model   organisms   and   three-­‐dimensional   (3D)   cell   culture   models   wherein   mammalian  epithelial  cells  undergo  morphogenesis  to  form  mul7cellular  spheres  with  a   hollow   central   lumen.   However,   the   mechanophysical   proper7es   associated   with   epithelial  morphogenesis  are  poorly  understood.     Write  the  background  sec<on  second   ü  Explain  why  this  study  needed  to  be  done   Problem   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  25. 25. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract We  performed  mul7dimensional  live-­‐cell  imaging  analysis  to  track  the  morphogene7c   process   star7ng   from   a   single   cell   to   the   development   of   a   mul7cellular,   spherical   structure  composed  of  polarized  epithelial  cells  surrounding  a  hollow  lumen.     Write  the  methods  sec<on  third   ü  General  techniques  used  to   obtain  the  presented  results   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  26. 26. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract Thus,   coordinated   rota7onal   movement   is   a   unique   mechanophysical   process   observed  during  normal  3D  morphogenesis  that  regulates  laminin  matrix  assembly   and  is  lost  in  cancer-­‐derived  epithelial  cells.   Write  the  conclusion  sec<on  last   ü  Major  conclusion  that  answers  the  problem   ü  Implica<ons  for  the  readers   However,   the   mechanophysical   proper<es   associated   with   epithelial  morphogenesis  are  poorly  understood.   Implica<ons   Conclusion   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  27. 27. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract Thus,   coordinated   rota7onal   movement   is   a   unique   mechanophysical   process   observed   during   normal   3D   morphogenesis   that   regulates   laminin   matrix   assembly   and   is   lost   in   cancer-­‐derived   epithelial  cells.   We   report   that   in   addi7on   to   ac7vely   maintaining   apicobasal   polarity,   the   structures   underwent   rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the  structures  rotated  360°  every  4  h  during  the  early   phase  of  morphogenesis.  Rota7onal  mo7on  was  independent  of  the  cell  cycle,  but  was  blocked  by   loss  of  the  epithelial  polarity  proteins  Scribble  or  Pard3,  or  by  inhibi7on  of  dynein-­‐based  microtubule   motors.   Interes7ngly,   none   of   the   structures   derived   from   human   cancer   underwent   rota7onal   mo7on.   We   found   a   direct   rela7onship   between   rota7onal   mo7on   and   assembly   of   endogenous   basement  membrane  matrix  around  the  3D  structures,  and  that  structures  that  failed  to  rotate  were   defec7ve  in  weaving  exogenous  laminin  matrix.  Dissolu7on  of  basement  membrane  around  mature,   nonrota7ng  acini  restored  rota7onal  movement  and  the  ability  to  assemble  exogenous  laminin.   We   performed   mul7dimensional   live-­‐cell   imaging   analysis   to   track   the   morphogene7c   process   star7ng  from  a  single  cell  to  the  development  of  a  mul7cellular,  spherical  structure  composed  of   polarized  epithelial  cells  surrounding  a  hollow  lumen.     Our  understanding  of  the  mechanisms  by  which  ducts  and  lobules  develop  is  derived  from  model   organisms   and   three-­‐dimensional   (3D)   cell   culture   models   wherein   mammalian   epithelial   cells   undergo  morphogenesis  to  form  mul7cellular  spheres  with  a  hollow  central  lumen.  However,  the   mechanophysical  proper7es  associated  with  epithelial  morphogenesis  are  poorly  understood.     Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  28. 28. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract Our  understanding  of  the  mechanisms  by  which  ducts  and  lobules  develop  is  derived  from  model   organisms   and   three-­‐dimensional   (3D)   cell   culture   models   wherein   mammalian   epithelial   cells   undergo  morphogenesis  to  form  mul7cellular  spheres  with  a  hollow  central  lumen.  However,  the   mechanophysical  proper7es  associated  with  epithelial  morphogenesis  are  poorly  understood.  We   performed  mul7dimensional  live-­‐cell  imaging  analysis  to  track  the  morphogene7c  process  star7ng   from  a  single  cell  to  the  development  of  a  mul7cellular,  spherical  structure  composed  of  polarized   epithelial   cells   surrounding   a   hollow   lumen.   We   report   that   in   addi7on   to   ac7vely   maintaining   apicobasal  polarity,  the  structures  underwent  rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the   structures  rotated  360°  every  4  h  during  the  early  phase  of  morphogenesis.  Rota7onal  mo7on  was   independent  of  the  cell  cycle,  but  was  blocked  by  loss  of  the  epithelial  polarity  proteins  Scribble  or   Pard3,  or  by  inhibi7on  of  dynein-­‐based  microtubule  motors.  Interes7ngly,  none  of  the  structures   derived  from  human  cancer  underwent  rota7onal  mo7on.  We  found  a  direct  rela7onship  between   rota7onal   mo7on   and   assembly   of   endogenous   basement   membrane   matrix   around   the   3D   structures,  and  that  structures  that  failed  to  rotate  were  defec7ve  in  weaving  exogenous  laminin   matrix.   Dissolu7on   of   basement   membrane   around   mature,   nonrota7ng   acini   restored   rota7onal   movement  and  the  ability  to  assemble  exogenous  laminin.  Thus,  coordinated  rota7onal  movement   is   a   unique   mechanophysical   process   observed   during   normal   3D   morphogenesis   that   regulates   laminin  matrix  assembly  and  is  lost  in  cancer-­‐derived  epithelial  cells.   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  29. 29. Coverage  and   Staffing  Plan   Manuscript   structure   Writing your abstract Our  understanding  of  the  mechanisms  by  which  ducts  and  lobules  develop  is  derived  from  model   organisms   and   three-­‐dimensional   (3D)   cell   culture   models   wherein   mammalian   epithelial   cells   undergo  morphogenesis  to  form  mul7cellular  spheres  with  a  hollow  central  lumen.  However,  the   mechanophysical  proper7es  associated  with  epithelial  morphogenesis  are  poorly  understood.  We   performed  mul7dimensional  live-­‐cell  imaging  analysis  to  track  the  morphogene7c  process  star7ng   from  a  single  cell  to  the  development  of  a  mul7cellular,  spherical  structure  composed  of  polarized   epithelial   cells   surrounding   a   hollow   lumen.   We   report   that   in   addi7on   to   ac7vely   maintaining   apicobasal  polarity,  the  structures  underwent  rota7onal  mo7ons  at  rates  of  15–20  μm/h  and  the   structures  rotated  360°  every  4  h  during  the  early  phase  of  morphogenesis.  Rota7onal  mo7on  was   independent  of  the  cell  cycle,  but  was  blocked  by  loss  of  the  epithelial  polarity  proteins  Scribble  or   Pard3,  or  by  inhibi7on  of  dynein-­‐based  microtubule  motors.  Interes7ngly,  none  of  the  structures   derived  from  human  cancer  underwent  rota7onal  mo7on.  We  found  a  direct  rela7onship  between   rota7onal   mo7on   and   assembly   of   endogenous   basement   membrane   matrix   around   the   3D   structures,  and  that  structures  that  failed  to  rotate  were  defec7ve  in  weaving  exogenous  laminin   matrix.   Dissolu7on   of   basement   membrane   around   mature,   nonrota7ng   acini   restored   rota7onal   movement  and  the  ability  to  assemble  exogenous  laminin.  Thus,  coordinated  rota7onal  movement   is   a   unique   mechanophysical   process   observed   during   normal   3D   morphogenesis   that   regulates   laminin  matrix  assembly  and  is  lost  in  cancer-­‐derived  epithelial  cells.  Conclusions   Results   Methods   Background   Wang  et  al.  PNAS.  2013;  110:  163‒168.  
  30. 30. Coverage  and   Staffing  Plan   Manuscript   structure   Introduction General  introduc<on   Specific  aims  Aims   Current  state  of  the  field   Problem  in  the  field   Secondary  introduc<on   Current  state  of  the  field   Problem  in  the  field  
  31. 31. Coverage  and   Staffing  Plan   Manuscript   structure   Introduction – flow of information Lung  cancer  is  the  leading  cause  of  cancer  mortality  for  men  and  women.  Despite  smoking  preven7on  and  cessa7on  programs  and  advances  in  early   detec7on,  the  5-­‐year  survival  rate  for  lung  cancer  is  only  16%  with  current  therapies.  Although  lung  cancer  incidence  rates  have  recently  declined  in  the   United  States,  more  lung  cancer  is  now  diagnosed  when  considered  together  in  former-­‐  and  never-­‐smokers  than  in  current  smokers.  Thus,  even  if  all  of  the   na7onal  an7-­‐smoking  campaign  goals  are  met,  lung  cancer  will  remain  a  major  public  health  problem  for  decades.  New  ways  to  treat  or  prevent  lung  cancer   are  therefore  needed.     One  poten7al  therapeu7c  target  for  lung  cancer  is  the  Wnt  signaling  pathway.  The  canonical  Wnt  signaling  pathway  in  mammals  consists  of  a  family  of   secreted  lipid-­‐modified  Wnt  protein  ligands  that  bind  to  a  family  of  7-­‐pass  transmembrane  Frizzled  (Fzd)  receptors,  as  reviewed.  In  brief,  in  the  absence  of   ligand,  glycogen  synthase  kinase-­‐3  (GSK3),  in  complex  with  axin  and  adenomatous  polyposis  coli  (APC),  cons7tu7vely  phosphorylates  β-­‐catenin,  the  primary   Wnt   signaling   effector,   targe7ng   it   for   ubiqui7na7on   and   proteasomal   destruc7on.   Ligand   binding   engages   a   pathway   involving   Dishevelled   (Dvl)   that   inhibits  GSK3,  allowing  β-­‐catenin  to  accumulate  in  a  hypophosphorylated  form.  This  stabilized  form  of  β-­‐catenin  can  translocate  to  the  nucleus,  where  it   ac7vates  target  gene  transcrip7on  by  complexing  with  T  cell  factor  (TCF)  and  lymphoid  enhancer-­‐binding  factor  (LEF).  In  addi7on  to  key  mediators  of   embryonic  development,  these  target  genes  include  cri7cal  growth-­‐regulators  such  as  myc  and  cyclin  D1.       Aberrant  Wnt  signaling  due  to  muta7ons  in  β-­‐catenin  or  APC  drives  deregulated  growth  in  both  familial  and  non-­‐hereditary  colorectal  cancers.  However,   non-­‐small  cell  lung  cancers  (NSCLC),  the  most  common  type  of  lung  cancer,  rarely  harbor  APC  or  β-­‐catenin  muta7ons.  Rather,  aberrant  Wnt  ac7vity  in  lung   cancer  is  linked  to  increased  expression  of  upstream  Wnt  signaling  effectors  such  as  Dvl  or  decreased  expression  of  Wnt  antagonists  such  as  Wnt-­‐inhibitory   factor  1  (Wif-­‐1).       Effec7ve  pharmacological  inhibitors  of  the  Wnt  pathway  have  only  recently  become  available.  Screens  for  small-­‐molecule  antagonists  of  the  Wnt  pathway   found  two  enzymes  to  be  key  mediators  of  Wnt  signaling.  These  are  poly-­‐ADP-­‐ribose  polymerase  (PARP)  enzymes,  tankyrase  (TNKS)  1  and  TNKS2,  which   anach  poly-­‐ADP-­‐ribose  (PAR)  onto  substrate  proteins.  Their  roles  in  regula7ng  telomerase  func7on  and  mito7c  spindle  forma7on  are  known,  but  their  role   in  PARsyla7ng  axin  so  as  to  maintain  the  op7mal  level  for  canonical  Wnt  signaling  has  only  recently  been  recognized.  The  compounds  iden7fied  in  these   screens,  XAV939,  IWR-­‐1  exo,  and  IWR-­‐1  endo,  act  by  specifically  inhibi7ng  the  PARP  ac7vity  of  TNKS1  and  TNKS2.  IWR-­‐exo  is  a  stereoisomer  of  IWR-­‐1  endo   with   ~14-­‐fold   lower   EC50.   PARP   inhibi7on   is   a   tractable   pharmacological   target   in   vivo,   as   antagonists   of   other   PARP   homologs   exert   an7neoplas7c   responses  in  breast  and  ovarian  cancer,  as  reviewed.       This  study  explored  the  hypothesis  that  inhibi7on  of  TNKS  by  pharmacological  or  gene7c  means  would  inhibit  lung  cancer  growth  in  vitro  and  in  vivo  in   clinically-­‐relevant  transgenic  mouse  models  of  lung  cancer  that  were  previously  developed,  as  reviewed.     General  introduc<on   Secondary  introduc<on   Objec<ves   Busch  et  al.  BMC  Cancer.  2012;  13:  211.  
  32. 32. Coverage  and   Staffing  Plan   Manuscript   structure   Methods How  it  was   done   General  methods   Specific  techniques   (discuss  controls)   Data  analysis   Quan<fica<on  methods   Sta<s<cal  tests   What  was   used   Samples  or  par<cipants   Materials   How  it  was   analyzed   Sus  métodos  reflejan  su  diseño  experimental  
  33. 33. Coverage  and   Staffing  Plan   Manuscript   structure   Results 1.  Ini<al  observa<on   2.  Characteriza<on   3.  Applica<on   Each  subsec<on   corresponds  to     one  figure   What  you  found,  not   what  it  means   Logical  presenta<on   Subsec<ons   Factual  descrip<on   Presente  sus  resultados  en  un  orden  lógico  
  34. 34. Coverage  and   Staffing  Plan   Manuscript   structure   Display items Present  large  amount   of  data  quickly  and   efficiently   Keep  it  simple:  use   separate  panels  if   necessary   Must  be  able  to  stand   alone:  clear  labels   and  figure  legends   Usually  the  first  thing   readers  will  look  at   Figures,  graphs   &  tables  
  35. 35. Coverage  and   Staffing  Plan   Manuscript   structure   Figures/schematics Clear  indicators   Clear  figure  legend   Kindlin-­‐2   knockdown   and   focal   adhesion   localiza<on.   A.   Confocal   immunofluorescent   microscopy   with   an7-­‐β1   integrin   (green)   and   an7-­‐paxillin   (red)   on   C2C12   cells   transfected   with   RNAi   and   then   changed   to   differen7a7on   media  for  2  days.  Control  cells  (scr  RNAi)  show   linear   staining   consistent   with   localiza7on   to   costameres   (arrows),   as   well   as   punctate   focal   contact  staining  (arrowheads).  Conversely,  focal   contact   proteins   in   the   kindlin-­‐2   RNAi   cells   fail   to   form   linear   structures   and   instead   are   concentrated   in   unusual   appearing   puncta   (*).   (Scale  bar  =  20  μM).     Dowling  et  al.  (2008)  BMC  Cell  Biol  9:36.  
  36. 36. Coverage  and   Staffing  Plan   Manuscript   structure   Discussion Beginning   Middle   End   Summarize  key  findings     State  major  conclusion   Restate  major  conclusion   Applica<ons/implica<ons   Suggest  future  work   Interpret  results  in  context     of  other  studies   Describe  limita<ons  
  37. 37. Coverage  and   Staffing  Plan   Manuscript   structure   References Cite  all  statements  from    previously  published  works   Use  reference  management  sorware   EndNote,  Papers,  RefWorks,   Mendeley   Cite  broadly  from  different     groups  in  your  field  
  38. 38. Coverage  and   Staffing  Plan   Manuscript   structure   Linking your ideas General  background   Objec<ves   Methodology   Results  and  figures   Summary  of  findings   Implica<ons  for  the  field   Relevance  of  findings   Knowledge  gaps   Enlace  lógicamente  sus  ideas  en  su  inves3gación   Current  state  of  the  field   Introduc<on   Methods   Results   Discussion  
  39. 39. Coverage  and   Staffing  Plan   Manuscript   structure   Linking your ideas New  ways  to  treat  or  prevent  lung  cancer    are  therefore  needed.   This  study  explored  the  hypothesis  that  inhibi<on   of  TNKS…would  inhibit  lung  cancer  growth…   Pharmacological  or  gene<c  inhibi<on  of  TNKS1   and  TNKS2…reduces  lung  cancer  prolifera<on...     Problem   Objec<ves   Conclusion   Discussion   Introduc3on   Busch  et  al.  BMC  Cancer.  2012;13:211.  
  40. 40. Target  the  journal   Section 3
  41. 41. Customer  Service  Target  the  journal   Chlorophyll  is  present  in  many  plant  organs,  including  immature   fruit  where  it  is  usually  degraded  during  ripening.  Mature  green   kiwifruit   (Ac9nidia   deliciosa)   are   an   excep7on,   with   high   concentra7ons   of   chlorophyll   remaining   in   the   fruit   flesh.   In   gold-­‐fleshed   kiwifruit   (A.   chinensis),   chlorophyll   is   degraded   to   colourless   catabolites   upon   fruit   ripening,   leaving   yellow   carotenoids  visible.  We  have  iden7fied  candidate  genes  for  the   control  of  chlorophyll  degrada7on…   Pilkington  et  al.  Planta.  2012;  236:  1615−1628.   The  control  of  chlorophyll  levels  in  maturing  kiwifruit   Broad focused journal Planta  
  42. 42. Customer  Service  Target  the  journal   Chlorophyll  is  present  in  many  plant  organs,  including  immature   fruit  where  it  is  usually  degraded  during  ripening.  Mature  green   kiwifruit   (Ac9nidia   deliciosa)   are   an   excep7on,   with   high   concentra7ons   of   chlorophyll   remaining   in   the   fruit   flesh.   In   gold-­‐fleshed   kiwifruit   (A.   chinensis),   chlorophyll   is   degraded   to   colourless   catabolites   upon   fruit   ripening,   leaving   yellow   carotenoids  visible.  We  have  iden7fied  candidate  genes  for  the   control  of  chlorophyll  degrada7on…   Pilkington  et  al.  Planta.  2012;  236:  1615−1628.   The  control  of  chlorophyll  levels  in  maturing  kiwifruit   Broad  interest   Broad  interest   Broad focused journal Planta  
  43. 43. Customer  Service  Target  the  journal   Tradi7onal  weed  management,  such  as  7llage  and  irriga7on,  has  led   to   an   enhanced   maintenance   of   wetland   plant   species   in   fallow   paddy  fields.  Recent  herbicide  usage  and  improvements  in  irriga7on   and   drainage   systems   however   have   caused   habitat   loss   of   these   species,  especially  in  fields  on  open  lowlands…   Effects  of  <llage  and  irriga<on  on  the  occurrence  and  establishment   of  na<ve  wetland  plant  species  in  fallow  paddy  fields   Narrow focused journal Paddy  and  Water  Development   Takanose  et  al.  Paddy  Water  Environ.  2013;  11:  1−4.  
  44. 44. Customer  Service  Target  the  journal   Tradi7onal  weed  management,  such  as  7llage  and  irriga7on,  has  led   to   an   enhanced   maintenance   of   wetland   plant   species   in   fallow   paddy  fields.  Recent  herbicide  usage  and  improvements  in  irriga7on   and   drainage   systems   however   have   caused   habitat   loss   of   these   species,  especially  in  fields  on  open  lowlands…   Effects  of  <llage  and  irriga<on  on  the  occurrence  and  establishment   of  na<ve  wetland  plant  species  in  fallow  paddy  fields   Narrow focused journal Paddy  and  Water  Development   Takanose  et  al.  Paddy  Water  Environ.  2013;  11:  1−4.   Keywords  from  the  Aims  and  Scope  
  45. 45. Effec3ve  wri3ng   Section 4
  46. 46. Effec7ve  Wri7ng   High readability Your  reader  should…   Understand  your  logic  immediately   Not  have  to  read  slowly   Only  have  to  read  once  
  47. 47. Effec7ve  Wri7ng   1. Short sentences Reading  once…   4%  of  readers  can  understand  a  27-­‐word  sentence   75%  of  readers  can  understand  a  17-­‐word  sentence     Pinner  and  Pinner  (1998)  Communica9on  Skills   U<lice  sólo  una  idea  por  oracion   15–20  palabras  
  48. 48. Effec7ve  Wri7ng   Short sentences 30  words   The  largest  company,  a  Mexican  corpora<on  founded  in  1916   outside  of  Puebla  by  Miguel  Hernández,  was  considered  to  be   a  model  in  the  development  of  modern  employee  condi<ons   by  economists.  
  49. 49. Effec7ve  Wri7ng   Short sentences 30  words   The  largest  company,  a  Mexican  corpora<on  founded  in  1916   outside  of  Puebla  by  Miguel  Hernández,  was  considered  to  be   a  model  in  the  development  of  modern  employee  condi<ons   by  economists.  
  50. 50. Effec7ve  Wri7ng   Short sentences Economists  considered  the  largest  company  to  be  a  model  in   the   development   of   modern   employee   condi<ons.   This   company  was  a  Mexican  corpora<on  founded  in  1916  outside   of  Puebla  by  Miguel  Hernández.   16  words   15  words   One  idea  per  sentence  
  51. 51. Effec7ve  Wri7ng   2. Active voice Sentences  wriOen  in  the  ac<ve  voice  are:   simple   direct   clear   easy  to  read   The  mechanisms  regula<ng  tumor  growth  were  inves<gated.   Passive   We  inves<gated  the  mechanisms  regula<ng  tumor  growth.   Ac3ve   U3lice  la  voz  ac3va  para  ser  más  directos  
  52. 52. Effec7ve  Wri7ng   Active voice is preferred “Use  the  ac3ve  voice  when  it  is  less  wordy  and  more   direct  than  the  passive”.  (3rd  ed.,  pg.  42)   “Use  the  ac3ve  voice  rather  than  the  passive  voice…”.     www.apastyle.org/learn/faqs/effec7ve-­‐verb-­‐use.aspx   “As  a  maner  of  style,  passive  voice  is  typically,  but  not   always,  inferior  to  ac3ve  voice”.  (15th  ed.,  pg.  177)   “In  general,  authors  should  use  the  ac3ve  voice…”.   (10th  ed.,  pg.  320)   ACS  Style  Guide   APA  Style     Chicago  Style   Guide   AMA  Manual  of   Style  
  53. 53. Effec7ve  Wri7ng   3. Stress position Los  lectores  se  centran  en  el  final  de  la  oracion   para  iden3ficar  lo  que  es  importante   1.  You  deserve  a  raise,  but  the  budget  is  <ght.   Which  sentence  suggests  that  you     will  get  a  raise?   2.  The  budget  is  <ght,  but  you  deserve  a  raise.   hnp://wri7ngcenter.unc.edu/handouts/flow/  
  54. 54. Effec7ve  Wri7ng   Stress position The  budget  is  <ght,  but  you  deserve  a  raise.  Your  salary   will  increase  at  the  beginning  of  next  year.   La  posición  de  tensión  debe  introducir     el  tema  de  la  siguiente  oracio   hnp://wri7ngcenter.unc.edu/handouts/flow/  
  55. 55. Effec7ve  Wri7ng   Stress position The  budget  is  <ght,  but  you  deserve  a  raise.  Your  salary   will  increase  at  the  beginning  of  next  year.   La  posición  de  tensión  debe  introducir     el  tema  de  la  siguiente  oracio   hnp://wri7ngcenter.unc.edu/handouts/flow/   Stress  posi<on  
  56. 56. Effec7ve  Wri7ng   Stress position The  budget  is  <ght,  but  you  deserve  a  raise.  Your  salary   will  increase  at  the  beginning  of  next  year.   La  posición  de  tensión  debe  introducir     el  tema  de  la  siguiente  oracio   hnp://wri7ngcenter.unc.edu/handouts/flow/   Stress  posi<on   Topic  posi<on   La  posición  del  tema  iden3fica  de  qué  trata  la  oración  
  57. 57. Effec7ve  Wri7ng   4. Topic position The   pa3ent   went   to   the   hospital   to   see   a   gastroenterologist.  The  doctor  then  performed  a  series   of   diagnos3c   tests.   The   results   showed   the   pa<ent   suffered   from   a   bacterial   infec3on.   An3bio3cs   were   prescribed   to   treat   the   infec<on   before   the   pa<ent   developed  an  ulcer.     idea idea idea idea Topic  link sentence 2.5  <mes  more  common  in  English  than  in  Spanish   Simpson  JM.  J  Second  Lang  Writ.  2000;  9:  293–309.  
  58. 58. Effec7ve  Wri7ng   Manuscript structure Lung   cancer   is   the   leading   cause   of   cancer   mortality   for   men   and   women.   Despite   smoking   preven<on   and   cessa<on  programs…  …New  ways  to  treat  or  prevent  lung   cancer  are  therefore  needed.     One  poten<al  therapeu<c  target  for  lung  cancer  is  the  Wnt   signaling  pathway.  The  canonical  Wnt  signaling  pathway  in   mammals  consists  of  a…   Busch  et  al.  BMC  Cancer.  2012;  13:  211.  
  59. 59. Effec7ve  Wri7ng   Lung   cancer   is   the   leading   cause   of   cancer   mortality   for   men   and   women.   Despite   smoking   preven<on   and   cessa<on  programs…  …New  ways  to  treat  or  prevent  lung   cancer  are  therefore  needed.     One  poten<al  therapeu<c  target  for  lung  cancer  is  the  Wnt   signaling  pathway.  The  canonical  Wnt  signaling  pathway  in   mammals  consists  of  a…   Busch  et  al.  BMC  Cancer.  2012;  13:  211.   Topic  sentence   Topic  sentence   Stress  sentence   U<lice  la  posicion  de    tensión  y  tema    en  su  escrito  de   manera  lógica  al  presentar  la  información   Manuscript structure
  60. 60. Cover  lebers   Section 5
  61. 61. Coverage  and   Staffing  Plan   Cover  leners   Abstract:   First  impression  for  readers   Cover  leOers  are  the  first  impression  for   the  journal  editor   Significance   Relevance   Level  of  English   Interes<ng  to   their  readers?   Is  your  work   important?  
  62. 62. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter Marc  Lippman,  MD   Editor-­‐in-­‐Chief   Breast  Cancer  Research  and  Treatment     3  September  2013     Dear  Dr  Lippman,     Please   find   enclosed   our   manuscript   en7tled   “Evalua7on   of   the   Glasgow  prognos7c  score  in  pa7ents  undergoing  cura7ve  resec7on   for  breast  cancer  liver  metastases,”  which  we  would  like  to  submit   for  publica7on  as  an  Original  Ar7cle  in  Breast  Cancer  Research  and   Treatment.     Journal  editor’s  name   Manuscript  <tle   Publica<on  type   Indique  el  Gtulo  de  su  escrito  y  3po  de  publicación  
  63. 63. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter The   Glasgow   prognos7c   score   (GPS)   is   of   value   for   a   variety   of   tumours.   Several   studies   have   inves7gated   the   prognos7c   value   of   the   GPS   in   pa7ents   with   metasta7c   breast   cancer,   but   few   studies   have  performed  such  an  inves7ga7on  for  pa7ents  undergoing  liver   resec7on  for  liver  metastases.  Furthermore,  there  are  currently  no   studies  that  have  examined  the  prognos7c  value  of  the  modified  GPS   (mGPS)  in  these  pa7ents.  The  present  study  evaluated  the  mGPS  in   terms   of   its   prognos7c   value   for   postopera7ve   death   in   pa7ents   undergoing  liver  resec7on  for  breast  cancer  liver  metastases.   Explique  por  qué  su  inves<gación  deberia  de  hacerse   ü  Current  state  of  the  field   ü  Problem  researchers  are  facing  
  64. 64. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter The   Glasgow   prognos7c   score   (GPS)   is   of   value   for   a   variety   of   tumours.   Several   studies   have   inves7gated   the   prognos7c   value   of   the   GPS   in   pa7ents   with   metasta7c   breast   cancer,   but   few   studies   have  performed  such  an  inves7ga7on  for  pa7ents  undergoing  liver   resec7on  for  liver  metastases.  Furthermore,  there  are  currently  no   studies  that  have  examined  the  prognos7c  value  of  the  modified  GPS   (mGPS)  in  these  pa7ents.  The  present  study  evaluated  the  mGPS  in   terms   of   its   prognos7c   value   for   postopera7ve   death   in   pa7ents   undergoing  liver  resec7on  for  breast  cancer  liver  metastases.   Explique  por  qué  su  inves<gación  deberia  de  hacerse   ü  Current  state  of  the  field   ü  Problem  researchers  are  facing   Introduc<on   Problem   Objec<ves  
  65. 65. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter Resuma  sus  conclusiones  clave   ü  Briefly  describe  your  methodology   ü  Summarize  your  key  findings   A   total   of   318   pa7ents   with   breast   cancer   liver   metastases   who   underwent  hepatectomy  over  a  15-­‐year  period  were  included  in  this   study.   The   mGPS   was   calculated   based   on   the   levels   of   C-­‐reac7ve   protein   and   albumin,   and   the   disease-­‐free   survival   and   cancer-­‐ specific   survival   rates   were   evaluated   in   rela7on   to   the   mGPS.   Overall,  the  results  showed  a  significant  associa7on  between  cancer-­‐ specific  survival  and  the  mGPS  and  carcinoembryonic  an7gen  level,   and  a  higher  mGPS  was  associated  with  increased  aggressiveness  of   liver  recurrence  and  poorer  survival  in  these  pa7ents.  
  66. 66. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter A   total   of   318   pa7ents   with   breast   cancer   liver   metastases   who   underwent  hepatectomy  over  a  15-­‐year  period  were  included  in  this   study.   The   mGPS   was   calculated   based   on   the   levels   of   C-­‐reac7ve   protein   and   albumin,   and   the   disease-­‐free   survival   and   cancer-­‐ specific   survival   rates   were   evaluated   in   rela7on   to   the   mGPS.   Overall,  the  results  showed  a  significant  associa7on  between  cancer-­‐ specific  survival  and  the  mGPS  and  carcinoembryonic  an7gen  level,   and  a  higher  mGPS  was  associated  with  increased  aggressiveness  of   liver  recurrence  and  poorer  survival  in  these  pa7ents.   ü  Briefly  describe  your  methodology   ü  Summarize  your  key  findings   Methods   Key  results   Resuma  sus  conclusiones  clave  
  67. 67. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter This  study  is  the  first  to  demonstrate  that  the  preopera7ve  mGPS,  a   simple  clinical  tool,  is  a  useful  prognos7c  factor  for  postopera7ve   survival  in  breast  cancer  pa7ents  undergoing  cura7ve  resec7on  for   liver   metastases.   This   informa7on   is   immediately   clinically   applicable   for   surgeons   and   medical   oncologists   trea7ng   such   pa7ents.  As  a  premier  journal  covering  breast  cancer  treatment,  we   believe  that  Breast  Cancer  Research  and  Treatment  is  the  perfect   plazorm  from  which  to  share  our  results  with  all  those  concerned   with  breast  cancer.   Explique  por  qué  su  escrito  sería  interesante   para  los  lectores  de  una  revista  
  68. 68. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter This  study  is  the  first  to  demonstrate  that  the  preopera7ve  mGPS,  a   simple  clinical  tool,  is  a  useful  prognos7c  factor  for  postopera7ve   survival  in  breast  cancer  pa7ents  undergoing  cura7ve  resec7on  for   liver   metastases.   This   informa7on   is   immediately   clinically   applicable   for   surgeons   and   medical   oncologists   trea7ng   such   pa7ents.  As  a  premier  journal  covering  breast  cancer  treatment,  we   believe  that  Breast  Cancer  Research  and  Treatment  is  the  perfect   plazorm  from  which  to  share  our  results  with  all  those  concerned   with  breast  cancer.   Explique  por  qué  su  escrito  sería  interesante   para  los  lectores  de  una  revista   Conclusion   Relevance  
  69. 69. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter This  study  is  the  first  to  demonstrate  that  the  preopera7ve  mGPS,  a   simple  clinical  tool,  is  a  useful  prognos7c  factor  for  postopera7ve   survival  in  breast  cancer  pa7ents  undergoing  cura7ve  resec7on  for   liver   metastases.   This   informa7on   is   immediately   clinically   applicable   for   surgeons   and   medical   oncologists   trea7ng   such   pa7ents.  As  a  premier  journal  covering  breast  cancer  treatment,  we   believe  that  Breast  Cancer  Research  and  Treatment  is  the  perfect   plazorm  from  which  to  share  our  results  with  all  those  concerned   with  breast  cancer.   Use  palabras  clave  a  par<r  de  los  obje<vos  y     el  alcance  en  su  carta  de  presentación   Keywords  from  the  Aims  and  Scope  
  70. 70. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter We  confirm  that  this  manuscript  has  not  been  published  elsewhere   and  is  not  under  considera7on  by  another  journal.  All  authors  have   approved   the   manuscript,   including   authorship   and   order   of   authorship,  and  agree  with  submission  to  Breast  Cancer  Research   and  Treatment.  This  study  was  funded  by  the  Ministry  of  Health,   Labour   and   Welfare.   The   authors   have   no   conflicts   of   interest   to   declare.   Exponga  responsabilidad  legal  sobre  é<ca  de  la   inves<gación  y  la  publicación   ü  Disclaimers  related  to  publica3on  ethics   ü  Sources  of  Funding   ü  Conflicts  of  interest  
  71. 71. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter We  confirm  that  this  manuscript  has  not  been  published  elsewhere   and  is  not  under  considera7on  by  another  journal.  All  authors  have   approved   the   manuscript,   including   authorship   and   order   of   authorship,  and  agree  with  submission  to  Breast  Cancer  Research   and  Treatment.  This  study  was  funded  by  the  Ministry  of  Health,   Labour   and   Welfare.   The   authors   have   no   conflicts   of   interest   to   declare.   Exponga  responsabilidad  legal  sobre  é<ca  de  la   inves<gación  y  la  publicación   ü  Disclaimers  related  to  publica3on  ethics   ü  Sources  of  Funding   ü  Conflicts  of  interest   Funding   Ethics   Conflicts  of  interest  
  72. 72. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter We  would  like  to  recommend  the  following  reviewers  to  evaluate   our  manuscript:         1.  Reviewer  1  and  contact  informa7on   2.  Reviewer  2  and  contact  informa7on   3.  Reviewer  3  and  contact  informa7on   4.  Reviewer  4  and  contact  informa7on       Please  address  all  correspondence  to:     ü  Recommended  reviewers   ü  Author’s  contact  informa7on   Other  important  informa<on:  
  73. 73. Coverage  and   Staffing  Plan   Cover  leners   Building your cover letter We  would  like  to  recommend  the  following  reviewers  to  evaluate   our  manuscript:         1.  Reviewer  1  and  contact  informa7on   2.  Reviewer  2  and  contact  informa7on   3.  Reviewer  3  and  contact  informa7on   4.  Reviewer  4  and  contact  informa7on       Please  address  all  correspondence  to:     Recommend  reviewers   Other  important  informa<on:   ü  Recommended  reviewers   ü  Author’s  contact  informa7on   Contact  informa<on  
  74. 74. Coverage  and   Staffing  Plan   Cover  leners   A good cover letter Marc  Lippman,  MD   Editor-­‐in-­‐Chief   Breast  Cancer  Research  and  Treatment     3  September  2013     Dear  Dr  Lippman,   Please  find  enclosed  our  manuscript  en7tled  “Evalua7on  of  the  Glasgow  prognos7c  score  in  pa7ents  undergoing  cura7ve  resec7on  for  breast  cancer  liver  metastases,”  which  we  would  like  to  submit  for   publica7on  as  an  Original  Ar7cle  in  Breast  Cancer  Research  and  Treatment.  .     The  Glasgow  prognos7c  score  (GPS)  is  of  value  for  a  variety  of  tumours.  Several  studies  have  inves7gated  the  prognos7c  value  of  the  GPS  in  pa7ents  with  metasta7c  breast  cancer,  but  few  studies  have   performed  such  an  inves7ga7on  for  pa7ents  undergoing  liver  resec7on  for  liver  metastases.  Furthermore,  there  are  currently  no  studies  that  have  examined  the  prognos7c  value  of  the  modified  GPS  (mGPS)  in   these  pa7ents.  The  present  study  evaluated  the  mGPS  in  terms  of  its  prognos7c  value  for  postopera7ve  death  in  pa7ents  undergoing  liver  resec7on  for  breast  cancer  liver  metastases.   A  total  of  318  pa7ents  with  breast  cancer  liver  metastases  who  underwent  hepatectomy  over  a  15-­‐year  period  were  included  in  this  study.  The  mGPS  was  calculated  based  on  the  levels  of  C-­‐reac7ve  protein  and   albumin,  and  the  disease-­‐free  survival  and  cancer-­‐specific  survival  rates  were  evaluated  in  rela7on  to  the  mGPS.  Prognos7c  significance  was  retrospec7vely  analyzed  by  univariate  and  mul7variate  analyses.   Overall,  the  results  showed  a  significant  associa7on  between  cancer-­‐specific  survival  and  the  mGPS  and  carcinoembryonic  an7gen  level,  and  a  higher  mGPS  was  associated  with  increased  aggressiveness  of  liver   recurrence  and  poorer  survival  in  these  pa7ents.     This  study  is  the  first  to  demonstrate  that  the  preopera7ve  mGPS,  a  simple  clinical  tool,  is  a  useful  prognos7c  factor  for  postopera7ve  survival  in  breast  cancer  pa7ents  undergoing  cura7ve  resec7on  for  liver   metastases.  This  informa7on  is  immediately  clinically  applicable  for  surgeons  and  medical  oncologists  trea7ng  such  pa7ents.  As  a  premier  journal  covering  breast  cancer  treatment,  we  believe  that  Breast  Cancer   Research  and  Treatment  is  the  perfect  plazorm  from  which  to  share  our  results  with  all  those  concerned  with  breast  cancer.       We  confirm  that  this  manuscript  has  not  been  published  elsewhere  and  is  not  under  considera7on  by  another  journal.  All  authors  have  approved  the  manuscript,  including  authorship  and  order  of  authorship,   and  agree  with  submission  to  Breast  Cancer  Research  and  Treatment.  This  study  was  funded  by  the  Ministry  of  Health,  Labour  and  Welfare.  The  authors  have  no  conflicts  of  interest  to  declare.       We  would  like  to  recommend  the  following  reviewers  to  evaluate  our  manuscript:         Reviewer  1  and  contact  informa7on   Reviewer  2  and  contact  informa7on   Reviewer  3  and  contact  informa7on   Reviewer  4  and  contact  informa7on       Please  address  all  correspondence  to:       We  look  forward  to  hearing  from  you  at  your  earliest  convenience.       Yours  sincerely,  
  75. 75. Coverage  and   Staffing  Plan   Cover  leners   A good cover letter Marc  Lippman,  MD   Editor-­‐in-­‐Chief   Breast  Cancer  Research  and  Treatment     3  September  2013     Dear  Dr  Lippman,   Please  find  enclosed  our  manuscript  en7tled  “Evalua7on  of  the  Glasgow  prognos7c  score  in  pa7ents  undergoing  cura7ve  resec7on  for  breast  cancer  liver  metastases,”  which  we  would  like  to  submit  for   publica7on  as  an  Original  Ar7cle  in  Breast  Cancer  Research  and  Treatment.  .     The  Glasgow  prognos7c  score  (GPS)  is  of  value  for  a  variety  of  tumours.  Several  studies  have  inves7gated  the  prognos7c  value  of  the  GPS  in  pa7ents  with  metasta7c  breast  cancer,  but  few  studies  have   performed  such  an  inves7ga7on  for  pa7ents  undergoing  liver  resec7on  for  liver  metastases.  Furthermore,  there  are  currently  no  studies  that  have  examined  the  prognos7c  value  of  the  modified  GPS  (mGPS)  in   these  pa7ents.  The  present  study  evaluated  the  mGPS  in  terms  of  its  prognos7c  value  for  postopera7ve  death  in  pa7ents  undergoing  liver  resec7on  for  breast  cancer  liver  metastases.   A  total  of  318  pa7ents  with  breast  cancer  liver  metastases  who  underwent  hepatectomy  over  a  15-­‐year  period  were  included  in  this  study.  The  mGPS  was  calculated  based  on  the  levels  of  C-­‐reac7ve  protein  and   albumin,  and  the  disease-­‐free  survival  and  cancer-­‐specific  survival  rates  were  evaluated  in  rela7on  to  the  mGPS.  Prognos7c  significance  was  retrospec7vely  analyzed  by  univariate  and  mul7variate  analyses.   Overall,  the  results  showed  a  significant  associa7on  between  cancer-­‐specific  survival  and  the  mGPS  and  carcinoembryonic  an7gen  level,  and  a  higher  mGPS  was  associated  with  increased  aggressiveness  of  liver   recurrence  and  poorer  survival  in  these  pa7ents.     This  study  is  the  first  to  demonstrate  that  the  preopera7ve  mGPS,  a  simple  clinical  tool,  is  a  useful  prognos7c  factor  for  postopera7ve  survival  in  breast  cancer  pa7ents  undergoing  cura7ve  resec7on  for  liver   metastases.  This  informa7on  is  immediately  clinically  applicable  for  surgeons  and  medical  oncologists  trea7ng  such  pa7ents.  As  a  premier  journal  covering  breast  cancer  treatment,  we  believe  that  Breast  Cancer   Research  and  Treatment  is  the  perfect  plazorm  from  which  to  share  our  results  with  all  those  concerned  with  breast  cancer.       We  confirm  that  this  manuscript  has  not  been  published  elsewhere  and  is  not  under  considera7on  by  another  journal.  All  authors  have  approved  the  manuscript,  including  authorship  and  order  of  authorship,   and  agree  with  submission  to  Breast  Cancer  Research  and  Treatment.  This  study  was  funded  by  the  Ministry  of  Health,  Labour  and  Welfare.  The  authors  have  no  conflicts  of  interest  to  declare.       We  would  like  to  recommend  the  following  reviewers  to  evaluate  our  manuscript:         Reviewer  1  and  contact  informa7on   Reviewer  2  and  contact  informa7on   Reviewer  3  and  contact  informa7on   Reviewer  4  and  contact  informa7on       Please  address  all  correspondence  to:       We  look  forward  to  hearing  from  you  at  your  earliest  convenience.       Yours  sincerely,   Manuscript  informa<on   Background   Key  findings   Relevance   Disclaimers   Recommended  reviewers  
  76. 76. Coverage  and   Staffing  Plan   Cover  leners   Recommending reviewers “When   submiKng   your   paper,   you   must   provide   the   names,  affilia7ons,  and  valid  e-­‐mail  addresses  of  five  (5)   reviewers.   If   you   do   not   do   so,   your   paper   will   be   returned,  unreviewed.”   “Authors   are   requested   to   provide   the   names   and   full   addresses  (including  e-­‐mail  address)  of  up  to  four  poten7al   referees…”   Verifique  las  directrices  de  la  revista  para   determinar  cuántos  revisores  se  recomiendan  
  77. 77. Coverage  and   Staffing  Plan   Cover  leners   Recommending reviewers Where  to  find   them?   From  your  reading/references,   networking  at  conferences   How  senior?   Aim  for  mid-­‐level  researchers   Who  to  avoid?   Collaborators  (past  5  years),   researchers  from  same  ins<tu<on   Interna3onal  list:     1  or  2  from  Asia,  1  or  2  from  Europe,  and  1  or  2  from  the  Americas   Recomiende  inves3gadores  de  nivel     medio  de  diferentes  países  
  78. 78. Peer  Review   Section 6
  79. 79. Peer  Review   Improves your manuscript Get  involved  in  the     peer  review  process   La  revisión  por  pares  es  un  proceso     posi<vo  que  mejorará  su  manuscito  
  80. 80. Peer  Review   What reviewers are looking for The  science   The  manuscript   ü  Relevant  hypothesis   ü  Good  experimental  design   ü  Appropriate  methodology   ü  Good  data  analysis   ü  Valid  conclusions   ü  Logical  flow  of  informa<on   ü  Manuscript  structure  and  formazng   ü  Appropriate  references   ü  High  readability  
  81. 81. Peer  Review   Response   leOer   Responda  claramente  a  cada     comentario  de  sus  revisores   Easy  to  see   changes   Refer  to  line  and  page  numbers   Use  a  different  color  font   Highlight  the  text   Revision
  82. 82. Peer  Review   Writing a response letter Marc  Lippman,  MD   Editor-­‐in-­‐Chief   Breast  Cancer  Research  and  Treatment     3  September  2013     Dear  Dr  Lippman,     Re:  Resubmission  of  manuscript  reference  No.  WJS-­‐07-­‐5739     Please  find  anached  a  revised  version  of  our  manuscript  originally  en7tled  “Evalua7on  of  the  Glasgow  prognos7c   score  in  pa7ents  undergoing  cura7ve  resec7on  for  breast  cancer  liver  metastases,”  which  we  would  like  to   resubmit  for  considera7on  for  publica7on  in  the  Breast  Cancer  Research  and  Treatment.     The  reviewer’s  comments  were  highly  insighzul  and  enabled  us  to  greatly  improve  the  quality  of  our  manuscript.  In   the  following  pages  are  our  point-­‐by-­‐point  responses  to  each  of  the  comments.     Revisions  in  the  manuscript  are  shown  as  underlined  text.  In  accordance  with  the  first  comment,  the  7tle  has  been   revised  and  the  en7re  manuscript  has  undergone  substan7al  English  edi7ng.     We  hope  that  the  revisions  in  the  manuscript  and  our  accompanying  responses  will  be  sufficient  to  make  our   manuscript  suitable  for  publica7on  in  the  Breast  Cancer  Research  and  Treatment.   Address  editor  personally   Manuscript  ID  number   Thank  reviewers   Highlight  major  changes  
  83. 83. Peer  Review   Agreeing with reviewers Reviewer  Comment:  In  your  analysis  of  the  data  you  have  chosen   to   use   a   somewhat   obscure   fiGng   func9on   (regression).     In   my   opinion,  a  simple  Gaussian  func9on  would  have  sufficed.  Moreover,   the   results   would   be   more   instruc9ve   and   easier   to   compare   to   previous  results.   Response:   We   agree   with   the   reviewer’s   assessment   of   the   analysis.  Our  tailored  func7on,  in  its  current  form,  makes  it  difficult   to   tell   that   this   measurement   cons7tutes   a   significant   improvement   over   previously   reported   values.   We   describe   our   new  analysis  using  a  Gaussian  fiKng  func7on  in  our  revised  Results   sec7on  (Page  6,  Lines  12–18).  
  84. 84. Peer  Review   Reviewer  Comment:  In  your  analysis  of  the  data  you  have  chosen   to   use   a   somewhat   obscure   fiGng   func9on   (regression).     In   my   opinion,  a  simple  Gaussian  func9on  would  have  sufficed.  Moreover,   the   results   would   be   more   instruc9ve   and   easier   to   compare   to   previous  results.   Response:   We   agree   with   the   reviewer’s   assessment   of   the   analysis.  Our  tailored  func7on,  in  its  current  form,  makes  it  difficult   to   tell   that   this   measurement   cons7tutes   a   significant   improvement   over   previously   reported   values.   We   describe   our   new  analysis  using  a  Gaussian  fiKng  func7on  in  our  revised  Results   sec7on  (Page  6,  Lines  12–18).   Agreeing with reviewers Agreement   Revisions   Loca<on  
  85. 85. Peer  Review   Disagreeing with reviewers Reviewer  Comment:  In  your  analysis  of  the  data  you  have  chosen   to   use   a   somewhat   obscure   fiGng   func9on   (regression).     In   my   opinion,   a   simple   Gaussian   func9on   would   have   sufficed.   Moreover,   the   results   would   be   more   instruc9ve   and   easier   to   compare  to  previous  results.   Response:   Although   a   simple   Gaussian   fit   would   facilitate   comparison  with  the  results  of  other  studies,  our  tailored  func7on   allows   for   the   analysis   of   the   data   in   terms   of   the   Smith   model   [Smith   et   al.,   1998].   We   have   now   explained   the   use   of   this   func7on   and   the   Smith   model   in   our   revised   Discussion   sec7on   (Page  12,  Lines  2–6).  
  86. 86. Peer  Review   Reviewer  Comment:  In  your  analysis  of  the  data  you  have  chosen   to   use   a   somewhat   obscure   fiGng   func9on   (regression).     In   my   opinion,   a   simple   Gaussian   func9on   would   have   sufficed.   Moreover,   the   results   would   be   more   instruc9ve   and   easier   to   compare  to  previous  results.   Response:   Although   a   simple   Gaussian   fit   would   facilitate   comparison  with  the  results  of  other  studies,  our  tailored  func7on   allows   for   the   analysis   of   the   data   in   terms   of   the   Smith   model   [Smith   et   al.,   1998].   We   have   now   explained   the   use   of   this   func7on   and   the   Smith   model   in   our   revised   Discussion   sec7on   (Page  12,  Lines  2–6).   Disagreeing with reviewers Revisions   Loca<on   Evidence  
  87. 87. Peer  Review   Reviewer   comment:   The   authors   looked   for   polymorphisms   in   the  promoter  region  of  the  gene;  however,  they  didn't  evaluate   the  untranslated  regions.  That  is  one  of  my  concerns  about  this   methodology.   “Hidden” questions Rephrased   ques3on:   Why   didn’t   the   authors   evaluate   polymorphisms  in  the  untranslated  regions  of  the  gene?  
  88. 88. Peer  Review   Reviewer   comment:   The   authors   looked   for   polymorphisms   in   the  promoter  region  of  the  gene;  however,  they  didn't  evaluate   the  untranslated  regions.  That  is  one  of  my  concerns  about  this   methodology.   “Hidden” questions Response:   In   this   study,   we   decided   to   focus   on   the   promoter   region  of  this  gene  because  previous  studies  [Yajima  et  al.,  2010;   Jackson   et   al.,   2011]   have   shown   that   its   transcrip7on   was   par7cularly   affected.   This   has   now   been   clarified   in   the   Discussion  sec7on  of  our  manuscript  (Page  16,  Line  24–28).  
  89. 89. Peer  Review   Reviewer   comment:   The   authors   looked   for   polymorphisms   in   the  promoter  region  of  the  gene;  however,  they  didn't  evaluate   the  untranslated  regions.  That  is  one  of  my  concerns  about  this   methodology.   “Hidden” questions Response:   In   this   study,   we   decided   to   focus   on   the   promoter   region  of  this  gene  because  previous  studies  [Yajima  et  al.,  2010;   Jackson   et   al.,   2011]   have   shown   that   its   transcrip7on   was   par7cularly   affected.   This   has   now   been   clarified   in   the   Discussion  sec7on  of  our  manuscript  (Page  16,  Line  24–28).   Revisions   Loca<on   Evidence  
  90. 90. Summary   ü Advantages  of  publishing  in  English   ü Choosing  the  most  suitable  journal   ü Logically  presen<ng  informa<on  in  your  manuscript   ü Clearly  communica<ng  your  ideas  in  English   ü Communica<ng  with  journal  editors   ü Naviga<ng  peer  review  
  91. 91. Thank you! www.edanzedi<ng.com   Any questions? Follow  us  on  TwiOer   @JournalAdvisor   Download  and  further  reading   edanzedi<ng.com/entrepares   Like  us  on  Facebook   facebook.com/JournalAdvisor  

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