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Submitted to:- Dr. D.J. Ghodasara
Associate Professor,
Dept. of vet. Pathology, Anand
Submitted by:- Undhad Vishal
M.V.Sc....
Mast cell
 Mast cell or mastocyte is a normal component of connective
tissue
 Mast cell are quite pleomorphic & nucleus ...
Mast cell
Eosinophile
Basophile
Mastocytoma/Mastoma in Dog
 Mastocytoma is benign tumor of tissue mast cells
German “mast” means fattend or stuffed
“cyte...
Incidence
 6% of all tumors & 13% of skin tumors in dog
 Age:- 8 years
 Breed:- Boxer & Pug more common
 Sex:- Both se...
Sites
 Hindquarter are most common (thigh, groin, scrotum)
Gross morphology
 1 to 10 cm diameter
 Tumor present in derm...
FIGURE 1. A large pedunculated cutaneous mast cell tumor on a mixed-breed female dog.
FIGURE 2. female Shar-Peis at left h...
A large and invasive mast cell
tumor involving the left tarsus in
a female Labrador
Mast cell tumor on the hock of a
6-yea...
Mast cell tumor on left hind
limb of a dog
Large Mast cell tumor on the
right fore limb of a dog
Mast cell tumor on the inner thigh of a dog
Mast cell tumor of the paw
Mast cell tumor of the paw
Multiple cutaneous mast cell
tumors with peritumoral
edema and bleeding.
Preputial mast cell tumor with
peritumoral edema, bruising and
erythema.
Muzzle mast cell tumor.
Grade
I One tumor confined to the dermis without
regional lymph node involvement
II One tumor confined to the dermis with ...
Cell
characters
Mature Intermediate Anaplastic
Cell shape Round to avoid Round to avoid Pleomorphic
Cell size Uniform Vary...
 Presents of eosinophiles due to some immunological
reaction
 Focal area of collagen degeneration are present
 Vascular...
Growth & metastasis
 Least potentially malignant
 Correlation between degree of maturity of tumor &
eventual disseminati...
Jejunum of dog. Tumor cells are diffusely invading from the mucosal cell
layer to the serosa. Mucosal ulceration is visibl...
Stomach of dog Slight pleomorphic tumor cells include round to ovoid
nuclei with some pleomorphism. Numerous mitotic figur...
Monotonous population of mast cells with centrally located hyperchromatic
nuclei and few discernable cytoplasmic granules....
Boxer:-Mast cell tumor, well differentiated, Wright-Leishman stain. Well
differentiated mast cells have numerous, purple, ...
Cutaneous mast cell tumor (well differentiated), dog, Wright-Leishman
stain. The mast cells have numerous purple granules ...
Well differentiated mast cells, cutaneous neoplasm, dog, Wright-Leishman
stain. The neoplastic cells are of relatively uni...
Well differentiated cutaneous mast cell tumor, dog, hematoxylin & eosin
stain. The mast cells have a relatively uniform ap...
Intermediate differentiation, cutaneous mast cell tumor, dog, hematoxylin
& eosin stain. The mast cells exhibit mild aniso...
Well differentiated cutaneous mast cell tumor, dog, AgNOR stain. The mast
cells have 1-2 AgNORs per cell with an overall s...
Intermediate differentiation, cutaneous mast cell tumor, dog, AgNOR stain.
The mast cells have 1-7 AgNORs per cell with an...
Intermediate differentiation of mast cells, cutaneous neoplasm, dog,
Wright-Leishman stain. The mast cells are large with ...
Poorly differentiated mast cells, cutaneous neoplasm, dog, Wright-
Leishman stain. The mast cell (left) is large and has s...
Boxer:-Mast cell tumor, poorly differentiated, Wright-Leishman stain.
The cytoplasm contains fine but sparse purple granul...
Treatment
 Surgery
 Radiation therapy
 Cryosurgery
 Photodynamic therapy
 Chemotherapy
-Corticosteroids like predniso...
Mastocytoma in cat
 Two forms:-
1. Cutaneous/skin MCT
2. Histiocytic/visceral MCT
Incidence
 Second most common skin tum...
Multiple mast cell tumors in
the skin of a cat with the
visceral form of mast cell
tumor disease
Multiple mast cell tumors...
Histologic features
 Same to dog
 Some confusion between mastocytoma & eosinophilic
granuloma complex
 Skin & oral cavi...
Haired skin of cat:- Endocytosis of erythrocytes
by neoplastic mast cells
Lymph node, cat, Wright-Leishman stain. Metastatic mast cells exhibit
anisocytosis and anisokaryosis with a variable degre...
Mastocytoma of horse
Classification & incidence
 Two distinct form
1. Single cutaneous nodule
2. Disseminated multiple fo...
Histological features
 Variable sized aggregates of well differentiated mast cells
 Mitotic figures are rare
 Mature eo...
Mastocytoma of cattle, Sheep & pig
Cattle
 3% Of all cutaneous & subcutaneous tumors
 No any age, breed & sex predilecti...
Mast cell leukemia/Mstosarcoma
 Primarily in cat with involvement of bone marrow, blood &
spleen
 Age:- >8years
 Sex:- ...
Spleen, dog, Wright-Leishman stain. Two metastatic mast cells with
coarse purple granules are present
Bone marrow, dog, Wright-Leishman stain. Metastatic mast cells exhibit
anisocytosis and anisokaryosis.
Blood smear, dog, mast cell leukemia, Wright-Leishman stain. Cutaneous
neoplasms were not present.
Blood smear, dog, Wright-Leishman stain. Mast cell is present in the
blood smear of a dog with cutaneous neoplasms.
Blood smear, dog, Wright-Leishman stain. A degranulated mast cell (right)
is present in the blood smear of a dog with syst...
Dr.vishal mastocytoma
Dr.vishal mastocytoma
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Dr.vishal mastocytoma

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Transcript of "Dr.vishal mastocytoma"

  1. 1. Submitted to:- Dr. D.J. Ghodasara Associate Professor, Dept. of vet. Pathology, Anand Submitted by:- Undhad Vishal M.V.Sc. Scholar Dept. of vet. Pathology
  2. 2. Mast cell  Mast cell or mastocyte is a normal component of connective tissue  Mast cell are quite pleomorphic & nucleus are round or ovoid & basophilic granules  Cytoplasmic basophilic granules physiological active & two components secretes 1. Histamine 2. Heparin
  3. 3. Mast cell Eosinophile Basophile
  4. 4. Mastocytoma/Mastoma in Dog  Mastocytoma is benign tumor of tissue mast cells German “mast” means fattend or stuffed “cyte” comes from Greek “kytos” means hollow cell Etiology  Genetic predispose  golden/red coat color  Chronic immune over-stimulation that occurs in dogs with allergies or other inflammatory conditions.  There may be environmental factors, viruses or other undetermined contributors.  Middle age to older dogs are more likely to develop MCT's
  5. 5. Incidence  6% of all tumors & 13% of skin tumors in dog  Age:- 8 years  Breed:- Boxer & Pug more common  Sex:- Both sex same incidence Clinical characteristics  Gastric & duodenal ulcer  Focal glomarulonephritis  Defect in immune response  Defective blood coagulation
  6. 6. Sites  Hindquarter are most common (thigh, groin, scrotum) Gross morphology  1 to 10 cm diameter  Tumor present in dermis  Cut surface is grayish white  Some time well capsulated grossly
  7. 7. FIGURE 1. A large pedunculated cutaneous mast cell tumor on a mixed-breed female dog. FIGURE 2. female Shar-Peis at left hindlimb more aggressive and invasive mast cell tumors with invole mammary tissues. FIGURE 3. A hyperpigmented raised lesion involving the left lateral thigh region. FIGURE 4. A raised erythematous mucocutaneous lesion involving the preputial orifice on a castrated male pug.
  8. 8. A large and invasive mast cell tumor involving the left tarsus in a female Labrador Mast cell tumor on the hock of a 6-year-old boxer
  9. 9. Mast cell tumor on left hind limb of a dog Large Mast cell tumor on the right fore limb of a dog
  10. 10. Mast cell tumor on the inner thigh of a dog
  11. 11. Mast cell tumor of the paw Mast cell tumor of the paw
  12. 12. Multiple cutaneous mast cell tumors with peritumoral edema and bleeding.
  13. 13. Preputial mast cell tumor with peritumoral edema, bruising and erythema. Muzzle mast cell tumor.
  14. 14. Grade I One tumor confined to the dermis without regional lymph node involvement II One tumor confined to the dermis with regional lymph node involvement III Multiple dermal tumors or large infiltrating tumor with or without regional lymph node involvement IV Any tumor with distant metastases or recurrence with metastases
  15. 15. Cell characters Mature Intermediate Anaplastic Cell shape Round to avoid Round to avoid Pleomorphic Cell size Uniform Vary Vary Cytoplasmic border Well defined Indistinct Indistinct Nuclei Uniformly spherical Large, slight vesicular Large, vesicular & irregular Mitotic figures Rare Present Numerous Cell arrangement Loosely in cord or nest Cord Large sheets
  16. 16.  Presents of eosinophiles due to some immunological reaction  Focal area of collagen degeneration are present  Vascular lesion like hyalinization & fibrinoid degeneration of small arterioles  In 10% cases focal accumulation of lymphocyte & plasma cells Hematology  Increase serum gamma globulin  Rarely circulating mast cells
  17. 17. Growth & metastasis  Least potentially malignant  Correlation between degree of maturity of tumor & eventual dissemination of neoplastic mast cells  Internal dissemination of malignant tumor in following organs regional lymph nodes, spleen, liver, kidneys, lungs & heart Prognosis  It is estimated that 50% of surgically removed mast cell tumors will re-grow in the same area.  Prognosis is variable and depends on many factors including tumor location, histological grade and clinical stage
  18. 18. Jejunum of dog. Tumor cells are diffusely invading from the mucosal cell layer to the serosa. Mucosal ulceration is visible with tumor cell infiltration. HE.
  19. 19. Stomach of dog Slight pleomorphic tumor cells include round to ovoid nuclei with some pleomorphism. Numerous mitotic figures are visible (arrows). HE.
  20. 20. Monotonous population of mast cells with centrally located hyperchromatic nuclei and few discernable cytoplasmic granules. (HE, 10X)
  21. 21. Boxer:-Mast cell tumor, well differentiated, Wright-Leishman stain. Well differentiated mast cells have numerous, purple, cytoplasmic granules that partially obscure nuclear morphology.
  22. 22. Cutaneous mast cell tumor (well differentiated), dog, Wright-Leishman stain. The mast cells have numerous purple granules that partially obscure nuclear morphology
  23. 23. Well differentiated mast cells, cutaneous neoplasm, dog, Wright-Leishman stain. The neoplastic cells are of relatively uniform size and appearance with numerous fine purple granules.
  24. 24. Well differentiated cutaneous mast cell tumor, dog, hematoxylin & eosin stain. The mast cells have a relatively uniform appearance with heavy cytoplasmic granulation.
  25. 25. Intermediate differentiation, cutaneous mast cell tumor, dog, hematoxylin & eosin stain. The mast cells exhibit mild anisocytosis, anisokaryosis, decreased granulation, and increased mitotic activity
  26. 26. Well differentiated cutaneous mast cell tumor, dog, AgNOR stain. The mast cells have 1-2 AgNORs per cell with an overall score of 1.8. The potential for metastasis is low.
  27. 27. Intermediate differentiation, cutaneous mast cell tumor, dog, AgNOR stain. The mast cells have 1-7 AgNORs per cell with an overall score of 3.19. The potential for metastasis is very high.
  28. 28. Intermediate differentiation of mast cells, cutaneous neoplasm, dog, Wright-Leishman stain. The mast cells are large with pleomorphic cytoplasmic granulation that varies in size and abundance.
  29. 29. Poorly differentiated mast cells, cutaneous neoplasm, dog, Wright- Leishman stain. The mast cell (left) is large and has sparse cytoplasmic granulation.
  30. 30. Boxer:-Mast cell tumor, poorly differentiated, Wright-Leishman stain. The cytoplasm contains fine but sparse purple granules. A few eosinophils also are present.
  31. 31. Treatment  Surgery  Radiation therapy  Cryosurgery  Photodynamic therapy  Chemotherapy -Corticosteroids like prednisolone -Antihistaminic drugs -antitumor drugs likes Vinblastine, vincristine
  32. 32. Mastocytoma in cat  Two forms:- 1. Cutaneous/skin MCT 2. Histiocytic/visceral MCT Incidence  Second most common skin tumor (20% of all skin tumors)  Age:- 10 years  Breed:- Siamese cats  Sex:- Male>Female Sites & gross morphology  Head & neck region more common  Two type cutaneous growths:- 1. Firm 2. Diffuse
  33. 33. Multiple mast cell tumors in the skin of a cat with the visceral form of mast cell tumor disease Multiple mast cell tumors all over their body
  34. 34. Histologic features  Same to dog  Some confusion between mastocytoma & eosinophilic granuloma complex  Skin & oral cavity diffuse infiltration of eosinophiles & some extent mast cells in some lesions is called as eosinophilic granuloma complex Growth & metastasis  Potentially malignant than dog  50% primary cutaneous mastocytoma involvement of regional lymph node & liver, spleen & other visceral organs
  35. 35. Haired skin of cat:- Endocytosis of erythrocytes by neoplastic mast cells
  36. 36. Lymph node, cat, Wright-Leishman stain. Metastatic mast cells exhibit anisocytosis and anisokaryosis with a variable degree of fine metachromatic granulation.
  37. 37. Mastocytoma of horse Classification & incidence  Two distinct form 1. Single cutaneous nodule 2. Disseminated multiple focal mast cell lesion in skin  Age:- 7years  Sex:- Male > Female  Breed:- No predispose Site & gross morphology  Any where in body but more in head  2 to 20 cm diameter  Tumor confined to skin  Surface normal appearance, hairless or ulcerated
  38. 38. Histological features  Variable sized aggregates of well differentiated mast cells  Mitotic figures are rare  Mature eosinophiles are local or diffuse accumulation  Characteristic feature is focal area of necrosis contained eosinophiles & necrotic debris Etiology, transmission & metastasis  Unknown  Onchocerca spp. microfilariae  No transmission occurs  Metastasis not reported
  39. 39. Mastocytoma of cattle, Sheep & pig Cattle  3% Of all cutaneous & subcutaneous tumors  No any age, breed & sex predilection  1 to 10 cm diameter  Aggregates of mast cell in regional lymph node, spleen, liver, lungs, heart & kidneys  Also occurs in internal organs ( omentum, abomasum, tongue) without cutaneous involvement  Microscopically similar to other spp. Pig  Age:- 6 to 18 month  Limited to skin single or multiple  No metastasis Sheep  Vary little information regarding mastocytoma
  40. 40. Mast cell leukemia/Mstosarcoma  Primarily in cat with involvement of bone marrow, blood & spleen  Age:- >8years  Sex:- Male>Female  Splenomegaly (splenicmastocytosis), anemia, vomiting, GIT hyperirritability  Most notable gross lesion in cat are hugely enlarged chocolate brown spleen, wider spread small pale focai on liver, gastric & duodenal ulcer.  Diagnosis based on presence of mast cell in smear from blood & bone marrow  Mast cell 20u in diameter & round, centrally or eccentrically placed nucleus & cytoplasm packed with small, uniform, purple granules
  41. 41. Spleen, dog, Wright-Leishman stain. Two metastatic mast cells with coarse purple granules are present
  42. 42. Bone marrow, dog, Wright-Leishman stain. Metastatic mast cells exhibit anisocytosis and anisokaryosis.
  43. 43. Blood smear, dog, mast cell leukemia, Wright-Leishman stain. Cutaneous neoplasms were not present.
  44. 44. Blood smear, dog, Wright-Leishman stain. Mast cell is present in the blood smear of a dog with cutaneous neoplasms.
  45. 45. Blood smear, dog, Wright-Leishman stain. A degranulated mast cell (right) is present in the blood smear of a dog with systemic mastocytosis.
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