Hplc

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Hplc

  1. 1. Cairo UniversityFaculty of PharmacyDepartment of Pharmaceutical chemistry
  2. 2. • Ceftriaxone is a cephalosporin beta lactam antibiotic• used in the treatment of bacterial infections caused by susceptible,usually gram positive microorganisms.IntroductionNSONHONO CH3NSNH2H HOONaSNHNNCH3OOHNa+OH2.7/2
  3. 3. • The mobile phase consisting of a binary mixture of acetonitrile andbuffer adjusted to pH 3.5 with orthophosphoric acid.• Degassed by ultrasonic water bath and filtered.Mobile phase and solutions
  4. 4. • Five different concentrations (10, 25, 50, 75 and 100 μg/ml) ofCeftriaxone sodium solutions were prepared for linearity studies.• The responses were measured as peak areas and plotted againstconcentration.Calibration
  5. 5. • The values of the relativestandard deviation of fivereplicate injections of thestandard solutions lie well withinthe limits.Precision
  6. 6. • The values of the relativestandard deviation ofthree replicate injectionsof the standard solutionslie well within the limits.Accuracy
  7. 7. • The % Recovery for each level should be between 98.0 to 102.0%.From the experimental value the recovery was found to be withinthe limit (98.5%).Acceptance Criteria for accuracy
  8. 8. • Correlation coefficient should be not less than 0.999. From theexperimental value the correlation coefficient was found to be thesame.Linearity
  9. 9. • S/N Ratio value should be 3 for LOD. From the experimental valuethe S/N ratio was found to be 2.87Limit of detection
  10. 10. • S/N Ratio value should be 10 for LOQ solution. From theexperimental value the S/N ratio was found to be within limit i.e.10.54Limit of quantification
  11. 11. • When flow rate was varied at 0.7 ml/min to 0.9 ml/min it was foundthat the variation in flow rate does not affect the methodsignificantly.Robustness
  12. 12. RefrencesInternational Journal of Pharma SciencesVol. 2, No. 4 (2012): 84-90Research Article

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