Your SlideShare is downloading. ×
Hplc
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Hplc

230

Published on

Published in: Technology, Education
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
230
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
0
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. Cairo UniversityFaculty of PharmacyDepartment of Pharmaceutical chemistry
  • 2. • Ceftriaxone is a cephalosporin beta lactam antibiotic• used in the treatment of bacterial infections caused by susceptible,usually gram positive microorganisms.IntroductionNSONHONO CH3NSNH2H HOONaSNHNNCH3OOHNa+OH2.7/2
  • 3. • The mobile phase consisting of a binary mixture of acetonitrile andbuffer adjusted to pH 3.5 with orthophosphoric acid.• Degassed by ultrasonic water bath and filtered.Mobile phase and solutions
  • 4. • Five different concentrations (10, 25, 50, 75 and 100 μg/ml) ofCeftriaxone sodium solutions were prepared for linearity studies.• The responses were measured as peak areas and plotted againstconcentration.Calibration
  • 5. • The values of the relativestandard deviation of fivereplicate injections of thestandard solutions lie well withinthe limits.Precision
  • 6. • The values of the relativestandard deviation ofthree replicate injectionsof the standard solutionslie well within the limits.Accuracy
  • 7. • The % Recovery for each level should be between 98.0 to 102.0%.From the experimental value the recovery was found to be withinthe limit (98.5%).Acceptance Criteria for accuracy
  • 8. • Correlation coefficient should be not less than 0.999. From theexperimental value the correlation coefficient was found to be thesame.Linearity
  • 9. • S/N Ratio value should be 3 for LOD. From the experimental valuethe S/N ratio was found to be 2.87Limit of detection
  • 10. • S/N Ratio value should be 10 for LOQ solution. From theexperimental value the S/N ratio was found to be within limit i.e.10.54Limit of quantification
  • 11. • When flow rate was varied at 0.7 ml/min to 0.9 ml/min it was foundthat the variation in flow rate does not affect the methodsignificantly.Robustness
  • 12. RefrencesInternational Journal of Pharma SciencesVol. 2, No. 4 (2012): 84-90Research Article

×