Class 2 Erythropoiesis

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Class 2 Erythropoiesis

  1. 1. ERYTHROPOIESIS DR. LAXMIKANTA SAY
  2. 2. HAEMOPOIESIS
  3. 3. DEFINITION  A process of origin, development maturation of erythrocytes. and CHANGES DURING ERYTHROPOIESIS  Reduction in size of cell From diameter 25 to 7.5µm  Disappearance of Nucleoli & nucleus  Appearance of Hemoglobin  Change in staining properties of cytoplasm
  4. 4. PRODucTION SITES AGE SITES Early weeks of Yolk Sac Embryonic life Middle of trimester Liver & spleen and lympnodes gestation Last trimester of gestation Bone marrow After Birth to 5 Years of life 5 to 20 years of life All bones More than 20 years Marrow of membranous bones as Vertebrae , sternum, ribs& iliac All bones except proximal portion of Humerus & Tibia
  5. 5. PRODucTION SITES
  6. 6. GENESIS OF ERYTHROPOIESIS
  7. 7. Haemocytoblast (stem Cell)   Cell size – 19-23 µm Nucleus - Very big - occupies almost whole of cell with open chromatin - 4-5 nucleoli  Cytoplasm - Rim all around the nucleus; deep basophilic  Mitosis – (++)
  8. 8. PRO-ERYTHROBLAST(NORMOBLAST)  Cell size - 14-19 µm Nucleus - very big, - occupies ¾ of cell volume - 2-3 nucleoli; chromatin open  Cytoplasm - scanty , only a rim around the nucleus; deep basophilic 
  9. 9. EARLY -NORMOBLAST    Cell size 10-14µm Nucleus - size decreases than its precursor; - chromatin further condenses Cytoplasm - amount increases, less basophilic
  10. 10. INTERMEDIATE NORMOBLAST    Cell size - 10-14µm Nucleus -size further decrease; chromatin further condenses Cytoplasm - marked, - polychromatophilic staining due to appearance of haemoglobin
  11. 11. LATE NORMOBLAST Early Normoblast Cell size 7-10µm  Nucleus size - very small with chromatin dot cart wheel appearance  Cytoplasm - markedly increase  Haemoglobin – further increases in amount  Late Normoblast Cell size 7 – 8 µm  Nucleus - degenerates, becomes uniformly stained pyknotic  Cytoplasm - further increase; more acidic, less basophilic  Haemoglobin – further increases in amount 
  12. 12. RETICULOCYTES Immature RBC slightly larger than mature RBC.  Cell size - 7-8 µm, No nucleus - Named due to presence of reticular network or reticulum of disintegrated organelle.  Cytoplasm - Acidophilic  Newborn counts 2-6%,less than 1% in normal adults. 
  13. 13. MATURE ERYTHROCYTE     Reticular network disappears & cell become matures RBC & attains shape biconcave shape. Mature RBC is with hemoglobin & with out nucleus. Requires 9 days for development & maturation of RBC . Requires 7 days to the stage of reticlocyte and 2 days for maturation to RBC.
  14. 14. CONTROL OF ERYTHROPISIS  Tissue oxygenation -blood flow -blood hemoglobin concentration -hemoglobin oxygen saturation       EPO(Erythropoietin) Hematopoietic Cytokines Colony stimulating factors GM-CSF(Granulocyte macrophage colony stimulating factor) Thrombopoietin and stem cell factor IL-1,IL-3,IL-IL-6
  15. 15. BIO-SYNTHESIS OF HEMOGLOBIN  FOR HEMOGLOBIN GENERAL FACTORS FORMATION -Erythropoietin - First class protein & amino -Thyroxine acids -Hemopoietic growth factors - Iron, Copper, -Vitamins. - Cobalt & Nickel  MATURATION FACTORS  VITAMINS -Vit.B12 -Vit-C -Intrinsic factor -Riboflavin -Folic acid -Nicotinic acid -Pyridoxin 
  16. 16. Erythropoietin      Haemopoietin or Erythrocyte stimulating factor Chemistry - glycoprotein ( 74% protein & 26% carbohydrate) - contains 165 aminoacids - MW. 46,000 Source of secretion - interstial cells of peritubular capillaries of kidney, also in Liver & Brain Stimulant - Hypoxia Actions of Erythropoietin - production, development & release of mature RBCs.
  17. 17. EFFECT OF HYPOXIA
  18. 18. THANK YOU

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