Your SlideShare is downloading. ×
  • Like
Sdrajani
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×

Now you can save presentations on your phone or tablet

Available for both IPhone and Android

Text the download link to your phone

Standard text messaging rates apply

Sdrajani

  • 60 views
Published

 

Published in Healthcare
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Be the first to comment
    Be the first to like this
No Downloads

Views

Total Views
60
On SlideShare
0
From Embeds
0
Number of Embeds
0

Actions

Shares
Downloads
1
Comments
0
Likes
0

Embeds 0

No embeds

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
    No notes for slide

Transcript

  • 1. GUIDED BY: DESAI P. B DIRECTOR & HEAD DEPARTMENT OF MICROBIOLOGY, SHREE RAMKRISHNA INSTI. OF COMP. EDU. & APPL. SCIENCES, SURAT. “IN VITRO STUDY ON MULTI DRUG RESISTANT Mycobacterium tuberculosis” PRESENTED BY RAJANI S. D. Lecturer LOKHAT SARVAJANIK TRUST, RAMPURA ,SURAT.
  • 2. INTRODUCTION Tuberculosis is an ancient disease & it remains the leading cause of death of human being. It is mainly caused by Mycobacterium tuberculosis Mycobacteria causing human disease may be classified as follows: Mycobacteria a). Typical tubercle bacilli. Human type M.tuberculosis. Bovine type M.bovis. Vole type M.microti. Human type M.africanum.
  • 3. b). Atypical mycobacteria (Runyon’s classification) Group 1 Photochromogen M. kansasii M.marinum M. simiae M. asiaticum Group 2 Scotochromogen M.Scrofulsceum M. Szulgai M. Xenopi M.Gordonae M. Celatum
  • 4. Group 3 Non photochromogens M.avium-intracellular complex M. Paratuberculosis M.terrae-triviale M.Shimoidae Group 4 Rapid growers M.fortuitum/ chelonae complex M.thermoresistible
  • 5. C). Saprophytic mycobacteria.  M.smegmatis  M.phlei  M.stercoris  M. thermo
  • 6. National 75 North Zone 95 East Zone 65 West Zone 80** South Zone 50
  • 7. EPIDEMIOLOGY  The average prevalence of tuberculosis in India is estimated to be 5.05 per thousands.  Prevalence of smear positive cause 2.7 per thousand and average.  Annual incidence of smear positive cases at 84 per 1, 00,000 annually. [2]  The recent survey of WHO Says 98% of cases in developing countries with an increase of ~3% annually, 10% in African countries.  80% of cases seen in 22 countries; about half in 5 countries: India, China, Indonesia, Nigeria and Bangladesh.  In India every day more than 5,000 develop TB disease and more than 1,000 people die. [3] It is estimated that yearly about 9 millions people in the world are attacked with TB, with 1.7 million deaths. [1]
  • 8. AIM OF STUDY To find out prevalence of Tuberculosis in South Gujarat region. Identification and characterization of MDR in TB cases. To find out MDR Pattern.
  • 9. WHAT IS MDR-TB & XDR TB Drug resistant TB is widespread and found in all countries surveyed. It emerges as a result of treatment mismanagement and is passed from person to person in the same way as drug sensitive TB. Multidrug resistant TB [MDR-TB] is a form of TB that does not respond to the standard six month treatment using first line drugs [i.e. resistant to Isoniazid and Rifampicin]. It can take two years to treat with drugs that are more toxic, and 100 times more expensive. If the drugs to treat MDR-TB are mismanaged. Further resistance can occur. Extensively drug resistant [XDR TB] is a form of TB caused by bacteria resistant to all the most effective drugs [i.e. MDR-TB plus resistance to any fluoroquinolone and any of the second line anti-TB injectable drugs: Amikacin, Kanamycin or Capreomycin].[18]
  • 10. [19]
  • 11. METHOD The present study was conducted during Jan., 2009 to Nov., 2009. We have analyzed all samples viz., sputum, pus, urine, fluid are collected and diagnosed by ZNCF stain and result noted as RNTCP gradation . All samples were digested and decontaminated by N-Acetyl-l-cysteine sodium hydroxide [12] and cultured in the L.J medium.[13]  Isolated colonies were studied in detail for their morphological, cultural and biochemical characteristics.[14]
  • 12. All positive culture samples, which were further tested for drug susceptibility towards first line drugs (rifampicin, isoniazid, streptomycin, ethambutol, pyrazinamide) and second line drugs (amikacin, kanamycin, ethionamide, sparfloxacin, levofloxacin, ciprofloxacin,) by MIC method. [15,16] . METHOD
  • 13. RESULT & DISCUSSION TABLE: 1 SAMPLE WISE DISTRIBUTION: SR.NO SAMPLE NO.OF SAMPLE 01 SPUTUM 43 02 PUS 30 03 URINE 09 04 FLUID 08 Table 1 shows that among total 90 samples, majority were of sputum samples. 0.00% 10.00% 20.00% 30.00% 40.00% 50.00% 60.00% SPUTUM PUS URINE FLUID PERCENTAGE[%] PERCENTAGE [%] Chart-1
  • 14. TABLE 2: DISTRIBUTION OF A.F.B. GRADATION BY RNTCP METHOD SR.NO GRADATION NO.OF SAMPLES 01 1+ 15 02 2+ 15 03 3+ 6 04 SCANTY 0 05 TOTAL 36 Table 2 shows among total 36 samples direct microscopic positive samples, majority sample were 1+ and 2+ . None of them was scanty. Chart-2
  • 15. TABLE 3: DISTRIBUTION BASED ON BIOCHEMICAL TEST GROUP NO.OF CULTURE BIOCHEMICAL TEST PNB CATALASE NIACIN M.tuberculosis 32 - - + MOTT 01 + + - Above table 3 suggested that total 33 strains, 32 strains were M.tuberculosis and 01 strain was MOTT. PERCENTAGE(%) M.tuberculosis MOTT Chart-3
  • 16. SR.NO GROUP NO.OF STRAIN 01 MDR 13 02 SENSITIVE TO ALL DRUGS 02 03 MONO DRUG & OTHER THAN MDR 18 TABLE 4: DISTRIBUTION BASED ON SENSITIVITY PATTEREN: Table 4 shows that total 33 strains, 13 strains were MDR, 02 strains were sensitive to all drugs and 18 strains were mono drug & other than MDR. MDR ,Means in my study resistant to Rifampicin and Isoniazid with or without any other drug. Mono drug resistant means resistant to any one drug .
  • 17. Chart-4
  • 18. TABLE 5: MDR PATTERN [13 STRAINS] DRUGS NO.OF STRAINS HR 4 HSR 3 HER 2 HESR 4 TOTAL 13 MDR Pattern suggested equal no of HR & HESR resistance i.e. 4 while no of HSR resistance was 3 and HER resistance was 2. 0.00% 5.00% 10.00% 15.00% 20.00% 25.00% 30.00% 35.00% HR HSR HER HESR PERCENTAGE(%) PERCENTAGE(%) Chart-5
  • 19. DISCUSSION In present study,4 types of specimens were included. From 90 specimens, only 47.77% (n=43) were sputum samples whereas 52.23% (n=47) were non respiratory tract category. Among them 40% were smear positive and 36.66% were culture positive. In our study, total 33 culture positive among them 96.96% were M.tuberculosis and 03.04 % were atypical Mycobacteria similar results were reported by Navarro V, Guix J, Roig P, Borras R. (10) We found, out of the total 33 positive culture, 39.39 % (n=13) were MDR strain found that is higher than compare with Surveillance of drug-resistant tuberculosis in the state of Gujarat, India in 2009. (11) Our results are similar to study conducted in Japan. At 2008 (19)
  • 20. CONCLUSION Our study shows that among isolated strains 96.96%were M.tuberculosis & 03.04% were atypical Mycobacteria.  The study revealed that direct smear positivity was 40. % and culture positivity was 36.36%, though tuberculosis has been clinically confirmed by physician but detail diagnosis was required to confirm the TB cases. Drug susceptibility testing was carried out with 33 culture positive isolates, 02 (6.06 %) strains were sensitive to all four drugs i.e., Rifampicin, Isoniazid, Ethambutol, Streptomycin. 13 ( 39.39%) strains were multi drug resistance, 18 (54.55%) strains were mono resistance and multi resist other than MDR. So, Prevalence of MDR-TB infection was more in South Gujarat region and is necessary to find out the cause of spread of MDR in this region.
  • 21. 1. S.Singh: Scaling up Anti Mycobacterial drug susceptibility services in India. It is high time, Indian Journal of Medical Microbio. [2008] 26 [3] : 209-11 REFERENCES 2. Epidemiology of Tuberculosis: Current status in India. A.K.Chakraborty.India J med Res. 120, oct-2004. p.p.248-276 3. Module for Laboratory Technicians.oct-2005 Central TB Division – New Delhi – 110011 4. Harries A, Maher D, Graham S.TB/HIV: A Clinical Manual. 2nd Edition. Geneva : WHO : 2004 WHO/HTM/TB/2004 : 329 5. Raviglione MC, Narain JP, Kochi A. HIV Associated Tuberculosis in developing Countries : Clinical Features, Diagnosis & Treatment, Bull WHO 1992;70: 515-25 6. Narain jp, Tripathy SP, Pontali E. TB & HIV Infection, Tuberculosis : Epidemiology & Control, New Delhi : WHO Regional office for South Ease Asia : 2002 7. Gothi D, Joshi JM, Clinical & Laboratory observations or tuberculosis at a Mumbai [India] clinic post grad Med j 2004;80:97-100
  • 22. 9. Corbett EL,Watt CJ, Walker N, Maher D, Williams BG, Raviglione MC, eta. The growing burden of tuberculosis; global trends & interactions with the HIV epidemic. Arch Intern. Med.2003;163 : 1009-21 10.Khatri GR, Friden TR, Controlling tuberculosis in India. N Engl .J med 2002; 347: 1420-5. 11.Gandhi N R, Moll A, Sturm A W , Pawinski R, Govender T, Laloo U, Redeller K, Andrews J,Firdlnnd G,2006 Extensively drug resistant tuberculosis as a cause of death in patient co infected with HIV and TB in a rural area in a south Africa. Lancet 368: 1575/1580 12.WHO [2006] Global Tuberculosis Control: Surveillance, Planning, Financing, WHO, Geneva, Switzerland. 13.Centres for Disease Control & Prevention [CDC] [2006] Emergence of M.tuberculosis with extensive resistance to 2nd line drugs world wide, 2000- 2004. Morb,wkly Rep 55:301-305 8. Sharma SK, Kadhiravan T, Banga A, Goyal T, Bhatia I, Saha PK, Spectrum of clinical disease in a cohort of 135 hospitalized HIV infected patients from north India, BMC Infec.Dis.2004;4:52
  • 23. 16.Antimicrobials in laboratory medicine edited by Ashok Rattan, BI,Churchill Livingstone 1st Edition [156-166] 17.U Raut, P Narang, DK Mendiratta, R Narang P.Narang, V.Deotale: Evaluation of Rapid MTT tube Method for detection of Drug Susceptibility of M.tuberculosis to Rifampicin & Isoniazid. 18.Paramshivam CN :An overview on drug resistant tuberculosis in India, India J tuber.1998;45:73-81 19.Anargyros, P., D. S. J. Astill, and I. S. Sim. 1990. Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens. J. Clin. Microbiol. 28:1288–1291 14.R.Vijdea, M.Stegger, A.sosnovskaja, A.B.Anderson V.Thomson, D.Bang, MDR- TB – Rapid detection of resistance of RIF & high or low levels of INH in clinical specimens & isolates: EURJ Clinical Micro. Infec.Dis. [2008]27: 1079-1086 15.Clinical Microbiology Procedures Handbook Editor in Chief, Henry D.Isenberg, Vol-I, American society for microbiology/WashingtonD.C.