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Bsc ii iv-vi sem imb

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  • 1. SYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE (Industrial Microbiology) 2011-12 onwardsB.Sc. SECOND SEMESTERINDUSTRIAL MICROBIOLOGYTools and Techniques in Microbiology(MM Theory 85 + CCE 15) Unit IMicroscopy:Invention of Microscope; History; Light Microscopy: Principle, Construction; Theory and applications of Bright Field Microscopy(Simple and Compound); Dark Field Microscopy; Phase Contrast Microscopy; UV and Fluorescent Microscopy; Electron Microscopy:Types of Electron Microscope (TEM & SEM); Preparation of Specimen, Advantages, Limitations and Applications; Use of Software inMicroscopy.Unit IIInstrumentation TechniquesBasic principle, construction and applications of Autoclave, Oven, Incubator, BOD, Laminar Air Flow, Colorimetry andSpectrophotometer; Centrifugation: Basic principles of sedimentation, methods and applications; Chromatography: General introduction,definition and types of chromatography, General principles underlying chromatographic technique, Working and applications of ThinLayer Chromatography.Unit IIIMicrometry and StainingOccular and Stage Micrometry: principle and application; Cell Count: Haemocytometry- principle and applications, Use of CameraLucida; Stains and Staining Techniques: Necessity of staining, Chemistry of dyes and stains, Fixation of Smears, Mordents andIntensifiers, Decolorizers; Theory of Staining: physical and chemicals; Types of Staining: Simple staining, Negative staining, Differentialstaining, Flagella, Cell-wall, Capsule staining, Gram staining, Acid Fast staining.Unit IVImmunological TechniquesZone phenomenon (Lattice hypothesis); Characteristics of Serological reactions; Neutralization, Precipitation: ring test, slide test andtube test, Agglutination: widal test, passive agglutination, Hemagglutination, Bacterial agglutination, Agglutination inhibition,Flocculation and Compliment Fixation; Principle and applications of Radial immunodiffusion, Double immunodiffusion andRadioimmunoassay (RIA), Immunoelectrophoresis.Unit VA. Biostatistics and BioinformaticsPrinciple of Biostatistics; Classification of Data; Tabulation and Graphical representation; Measure of Central Tendency: Mean, Mode,Median- merits and demerits; Measure of Dispersion Range; Mean Deviation Variance and Standard Deviation.B. Bioinformatics: Basic Organization of Computer; Computer Hardware; Software, Bit, Byte, Computer Memory, Binary Code, BinarySystem; Introduction to Bioinformatics, Database and applications of Bioinformatics.List of recommended books:1. Microbiology, Authors- Pelczar, Chan and Kreig.2. General Microbiology, Authors- Stainer, Ingharam, Wheelis and Painter3. Biology of Microorganisms, Authors- Brock and Madigan.4. Fundamental Principles of Bacteriology, Author- A.J. Salle.5. Introduction to Microbiology, Authors- Ingraham and Ingraham.6. Microbial Physiology, Authors- Moat and Foster.7. Tools and techniques in microbiology by Nath and Upadhyay8. Powar C. B. and H. F. Daginawala (2003).General Microbiology Vol.II; Himalaya Publishing House.9. Dubey R. C. and D. K. Maheshwari (2004). A Text book of microbiology, 1stEdition; S.Chand and Company Ltd.10. H.C. Dube (2005) A Textbook of Fungi, Vikas Publishing House.11. Textbook of biostatics by B.L Agarwal12. Textbook of biostatics by K.Agarwal13. Mishra and Mishra. Statistics.Approved by1. Board of Studies in Microbiology on 16/07/11 & Faculty of Life Science on 16/07/112. Approved by Board of Studies in Microbiology SAC, JBP on 19.07.2012
  • 2. SYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE (Industrial Microbiology) 2011-12 onwardsB.SC FOURTH SEMESTERINDUSTRIAL MICROBIOLOGYMolecular biology, Microbial Genetics and Biochemistry(MM Theory 85 + CCE 15)Unit INucleic acid: structure of DNA and RNA, replication of DNA, synthesis of RNA and their types. Genetic code, concept of genes. Proteinsynthesis: transcription and translation, Operon concept, gene regulation in eukaryotes, Britten Davidson model of gene expression.Unit IIGenetic recombination in bacteria, transformation, transduction and conjugation, genetic mapping, extrachromosomal genetic material,:plasmid, cosmid, transposons, overlapping genes, silent genes. Plasmid- Structure, properties and types of plasmids.Unit IIIMutation: Evidence for spontaneous nature of mutation. Molecular basis of mutation- Types of mutation. Mutagenic agents-Physical andchemical. Mutation rate, DNA damage and repair mechanism, Photoreactivation, Excision, Mismatch, SOS repair and Dealkylationrepair. Auxotrophs, Prototrophs and Ames test.Unit IVCarbohydrates: Chemical structures, nature and properties. Classification and importance in biological cells.Amino acids and Proteins: Amino acids- Classification and general properties. Structure, Zwitter ion nature. Proteins- Classification,Structure and function. Primary, secondary, tertiary and quaternary structure.Lipids: Saturated and unsaturated fatty acids. Structure, classification, properties and function of lipids. Distribution and functions oflipids in microorganisms.Unit VEnzymes: classification. Co-enzymes, co-factors, mechanism of enzyme action, competitive and non competitive inhibition, allostericregulation of enzymes, isoenzymes, factors contributing to catalytic efficiency of enzymes.Approved by1. Board of Studies in Microbiology on 16/07/11 & Faculty of Life Science on 16/07/112. Approved by Board of Studies in Microbiology SAC, JBP on 19.07.2012
  • 3. SYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE (Industrial Microbiology) 2011-12 onwardsList of recommended books:1. Principles of Biochemistry, Author- A.L. Lehniger2. Fundamentals of Biochemistry, Author- J. L. Jain3. Biochemistry, Author- Voet and Voet.4. Microbial Genetics, Authors- Freifelder.5. Textbook of Microbiology, Authors- Dubey and Maheshwari.6. Powar C. B. and G. R. Chatwal(1994). Biochemistry, 3rd Edition; Himalaya Publishing House,New Delhi.7. Powar C. B. and H. F. Daginawala (2003).General Microbiology Vol.I; Himalaya Publishing House8. Jain, J.L., S. Jain and N. Jain (2005) 6th Edition. Fundamental of Biochemistry. S. Chand and Co.9. Rama Rao, A.V.S.S. (2000) 8th Edition. Textbook of Biochemistry. UBS Publisher Ltd.Approved by1. Board of Studies in Microbiology on 16/07/11 & Faculty of Life Science on 16/07/112. Approved by Board of Studies in Microbiology SAC, JBP on 19.07.2012
  • 4. SYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE (Industrial Microbiology) 2011-12 onwardsSYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE(SESSION : 2009-10 onwards)SUBJECT - INDUSTRIAL MICROBIOLOGYSIXTH SEMESTERPAPER-IFERMENTATION TECHNOLOGY AND GOVERNMENT REGULATIONSTime: 3 hours Max. Marks : 35Unit- IFermentation equipments and production process. Principal types of fermenters- The batch fermenters, continuous and solid state fermenter.Unit- IIOther fermentation equipments and industrial uses. The fluidized bed fermenter, stirred tank fermenters. Tubular fermenter. Computer control offermentation process. Strain improvement process.Unit- IIIIndustrial production of organic acids- Lactic acid, citric acid and acetic acid production and their applications.Unit- IVRole of international organisation in biotechnology. Government programmes for biotechnology development. Government regulations of recombinantDNA research.Unit- VHazardous industrial wastes, Mycotoxin hazards in the production of fungal products. Regulations for disposal of biohazardous materials. Patenting ofthe products in industry.RECOMMENDED BOOKS :1. Fermentation technology by Whittakar.2. General microbiology, vol. II ,by Powar and Daginawala.3. Molecular biology and Biotechnology by H.D. Kumar.Approved by1. Board of Studies in Microbiology on 16/07/11 & Faculty of Life Science on 16/07/112. Approved by Board of Studies in Microbiology SAC, JBP on 19.07.2012
  • 5. SYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE (Industrial Microbiology) 2011-12 onwardsSYLLABUS FOR THE DEGREE OF BACHELOR OF SCIENCE(SESSION : 2009-10 onwards)SUBJECT - INDUSTRIAL MICROBIOLOGYSIXTH SEMESTERPAPER-IITime: 3 hours Max. Marks : 35INDUSTRIAL PRODUCTIONUnit- IIndustrial production and applications of antibiotics – Penicillin and Streptomycine. Industrial production and applications of vitamins – vitamin B12and Riboflavin.Unit- IIImportance of microorganisms in dairy industries. Production of Cheese and Buttermilk. In bakery industries – leavening of bread. Indianfermented foods.Unit- IIIMass production of single cell proteins (SCP) and proteins from bacteria, Cyanobacteria, fungi and their significance.Unit- IVIndustrial production of Enzymes – amylase and protease production and their applications.Unit- VIndustrial production of alcohol, wine, beer and Production of amino acids – L-lysine and glutamic acid.RECOMMENDED BOOKS :4. Industrial microbiology by L.E. Casida.5. Elements of Biotechnology by P.K. Gupta.6. Industrial Microbiology by A.H. Patel.7. Industrial Microbiology by Prescott and Dunn8. Industrial Microbiology by WaitesApproved by1. Board of Studies in Microbiology on 16/07/11 & Faculty of Life Science on 16/07/112. Approved by Board of Studies in Microbiology SAC, JBP on 19.07.2012

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