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BioChain Company Overview

BioChain Company Overview

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BioChain Company Overview Presentation Transcript

  • 1. BioChain Institute, Inc. Turn Ideas into Reality
    Bio-Sample Preparation for Life Science and Diagnostics
    1
  • 2. Company Profile
    Founded in 1994, Privately held
    Group Companies :
    Biochain Institute, Inc in Hayward, CA, USA
    Biochain(Beijing) Science-Tech Corp. in Beijing, China
    Beijing Modern Gaoda Biotechnology Co. in Beijing, China
    Branch over 35 distributors worldwide
    About 200 employees around the world
    Revenue has been growing annually in double digits.
    Profitable since 1999
    Over ten thousand customers, industry and academic
    Over 5000 products and providing various custom services
    ISO 9001, 13485 Certified & GMP
    2
  • 3. Facility
    USA Facility
    Location: 3517 Breakwater Avenue, Hayward, CA, USA
    Size: 10,000 sq ft
    Level: R&D and Manufacture facility
    China Facility
    Location: No.7A,Yongchang North Road, Economic and Technological Development Area, Beijing, China
    Size: 80,000 sq ft manufacture
    Level: Clinical Diagnostic GMP manufacture and laboratory
    3
  • 4. Facility - USA
    4
  • 5. Facility - USA
    .
    5
  • 6. Facility - China
    6
  • 7. Company Culture
    Respect
    Integrity
    Improvement
    Innovation
    Honor
    7
  • 8. Team
    Scientific Team
    35 Ph.D. and over 40 Master’s Degree scientists who are well trained in the related research and technology development field. Many of them have over 10 years of working experience in the industry.
    8
  • 9. Quality Policy
    • Business Growth.
    • 10. Customers’ Satisfaction.
    • 11. Quality Products.
    • 12. Continuous Improvement.
    9
  • 13. Quality Certificate - USA
    10
  • 14. Quality Certificate - China
    11
  • 15. Business Scope
    Biochain Institute Inc. USA
    Sample Preparation for Diagnostics & Life Science Research
    Biochain(Beijing) Science-Tech Corp.
    Molecular Diagnostics and Central Lab
    Beijing Modern Gaoda Biotechnology Co.
    Immunodiagnostics
    12
  • 16. Product Category
    DNA
    cDNA
    RNA
    Protein
    Tissue
    Antibody
    Cell & Cell Culture
    Biomarker
    Bioassay
    Enzymes
    Custom Service
    Diagnostic Kit
    13
  • 17. Nucleic Acid
    Extraction and Purification
    Gene Expression Profiling and Analysis
    Gene Amplification and Cloning
    Gene Regulation and Sequencing
    Proteins
    Extraction and Purification
    Protein Expression Profiling and Analysis
    Tissue Analysis & Antibodies
    Tissue Section and Array
    IHC and ISH
    Conjugated Antibodies
    Cell and Cell Culture
    Primary Cell and Culture Media
    Custom Service
    Preclinical Animal Service
    Cancer Patient Sample Collection and Analysis
    Custom Antibodies
    Molecular Biology Service
    Product Application for Life Science Research
    14
  • 18. Product Application forDiagnostics
    Molecular Diagnostics
    PCR
    Sequencing
    Immunodiagnostics
    ELISA
    Point of Care
    Strip - Colloidal Gold-Based Immunoassay
    Biochemical Diagnostics
    Biochemical Detector and Bioassay
    15
  • 19. Bio-Sample Preparation Process
    Sample Collection
    Storage & Transportation
    Extraction & Purification
    Extracts of Bio-Sample
    Detection and Analysis
    16
  • 20. Bio-Sample Collection
    Sample Resource
    From licensed venders and Hospitals with Supply Agreement
    Collected under strict IRB ethical consenting practices (human patient samples)
    Annotated with associated clinical data and pathological diagnosis
    Preserved using stringent protocols to maximize sample quality
    Species
    Human: Normal, Cancer, Diseases
    Animal: Monkey, Mouse, Nude Mouse, Rat, Rabbit, Hamster, Porcine, Guinea Pig, Bovine
    Plant: Arabidopsis , Corn , Wheat , Soy bean , Potato , Rice , Orange
    Type
    Tissue, Whole Blood, Serum, Plasma, Urine, Stool
    17
  • 21. Vast Tissue Variety
    Broadest tissue collection
    Brain
    Throat
    Thyroid
    Lymphoma
    Esophagus
    Lung
    Artery
    Breast
    Spleen
    Liver
    Bone marrow
    Gallbladder
    Stomach
    Kidney
    Vein
    Pancreas
    Skin
    Intestine, colon, rectum
    Bladder
    Ovary, Uterus, Cervix, Testis
    Prostate
  • 22. Total RNA, mRNA, microRNA
    Quality
    High quality RNA for high efficiency in PCR, reverse transcription, and microRNA studies
    Extensive quality control procedures
    MicroRNA certified total RNA
    Documentation of donors' clinical history available
    Variety
    Human normal, cancer (primary and metastasis) and diseased tissues & blood
    Matched Pairs: normal and primary pair (PP), primary and metastatic pair (PM)
    Control genomic DNA, human and animal
    Human cancer cell lines
    Animals and plants
    Format
    Single organ, single donor or multi-donors, male or female
    Multi-organs, single donor or multi-donors, male or female
    19
  • 23. Total RNA and mRNA
    Normal: >50 Organs
    Vast Variety
    Cancers: >40 Types
    Cancer Matched Pair: Normal & primary/primary and metastatic
    Neurological
    Diabetic
    Cancer cell lines: Hela, Jurkat, A431, K562, MCF7, Raji
    Human
    Cardiovascular
    Adult diseased
    Pulmonary
    Liver cirrhosis
    Lupus disease
    Total and message RNA
    Fetal normal: >30 Organs
    Animal: Bovine, Equine, Chicken, Dog, Mouse, Rat, Hamster,
    Guinea pig, Rabbit, Cynomolgus monkey, Rhesus monkey
    Plant
    Human (male and female): multiple organs
    Control Genomic DNA
    Animal (male and female): Bovine, Equine, Chicken, Dog, Mouse, Rat, Hamster, Guinea pig, Rabbit, Cynomolgus monkey, Rhesus monkey
    20
  • 24. Total RNA and mRNA
    Features
    • Isolated from a wide variety of normal and diseased tissues
    • 25. Applicable for microRNA regulation profiling
    • 26. Documentation of tissues clinical history available
    Application
    • cDNA synthesis and cDNA library construction
    • 27. Northern Blotting, RT-PCR, and RACE
    • 28. MicroRNA study
    • 29. cDNA probe for profiling study in gene expression
    • 30. RNA protection and primer extension
    • 31. RNA display
    • 32. Purification of mRNA
    21
  • 33. Sample Collection Service Scope
    Sample Collection
    • Body fluid and tumor samples according to various customer‘s
    specifications.  
    • Lung , Breast, Colon, Esophagus, Liver, Kidney, Stomach, Ovarian, Cervical,
    Uterus cancers. Other samples may be available upon request.
    Sample Preparation
    • DNA/RNA/Protein Isolation
    • 34. Tissue Sections and Tissue Arrays
    Sample Analysis
    • PCR Test for DNA and RNA
    • 35. Western Analysis of Protein lysate
    • 36. IHC and ISH test for tissue sections and tissue arrays
    22
  • 37. Clinical info collection
    23
  • 38. Collection Network
    Many hospitals in China
    Tissue vendors world wide
    BioChain
    Customers
    24
  • 39. Tissue arrays, sections, and panels
    Quality
    • Ready-to-use for IHC, ISH, FISH, LCM, etc
    • 40. Tissues selected by board-certified pathologists
    • 41. Tissues collected under strict IRB and ethical consenting practices
    • 42. Clinical data available including age, gender, tumor TNM and stage
    Variety
    In both paraffin-embedded/FFPE and frozen formats
    Human normal, cancer (primary and metastasis) and diseased tissues
    Matched Pairs: normal and primary pair (PP), primary and metastatic pair (PM)
    Human cancer cell lines
    Animals and plants
    25
  • 43. High-throughput tool for therapeutic and diagnostic antibody validation
    30 human normal organs arrayed according to FDA guidelines
    3 donors per organ for better statistics under FDA regulations
    Cost- and time-efficient screening of cross-reactivity
    FDA Standard FFPE Tissue Array available
    FDA Standard Frozen Tissue Array
    H &E stained FDA Standard Frozen Tissue Array. A total of 90 tissues spotted on 2 glass slides.
    Examples of BioChain’s Frozen Tissue Array OCT Blocks
    26
  • 44. Features
    Large cancer tissue collection
    Pre-arrayed and ready-to-use
    12 to 150 different tissues are spotted on one array
    In both paraffin-embedded/FFPE and frozen formats
    Multi-cancers, Multi-donors, Multi-applications
    Matched tissue DNA, RNA, protein and single sections available
    Clinical Information
    Tissue samples selected by board-certified pathologists, collected under strict IRB and ethical consenting practices
    Accompanied with pathological and clinical annotation including patient age, gender, tumor diagnosis, tumor stage (or TNM), tumor size, tumor differentiation, tumor history and H&E images of the source, etc
    Cancer Tissue Array
    27
  • 45. Selected cancer tissue arrays
    Cancer Tissue Array
    For complete product list, visit www.biochain.com or call 1-888-762-2568
    28
  • 46. FFPE and Frozen Tissue Sections
    Fig. 1 (left) In Situ Hybridization and Immunohistochemistry of p53 gene in human colon cancer tissue FFPE sections. Three serial paraffin embedded tissue sections were analyzed for p53 gene expression in human colon cancer tissue. It is shown that p53 mRNA and p53 protein are localized in the same area under 400x magnification.
    Top: H.E. stained image
    Middle: Image of immunohistochemistry
    Bottom: Image of in situ hybridization
    Fig. 2 (left) H. E. stained image of both paraffin embedded and frozen kidney tissue sections.
    Top: Paraffin embedded kidney section
    Bottom: Frozen kidney section
    Tumor Matched Pair sections - Same Donor – Adjacent normal and Primary tumor pair - Primary and Metastasis tumor pair
    A
    B
    C
    D
    Fig. 3 (above) H.E. stained tumor matched pair tissue sections.
    A: Paraffin embedded breast cancer tissue
    B: Paraffin embedded breast cancer adjacent normal tissue
    C: Frozen breast cancer tissue
    D: Frozen breast cancer adjacent normal tissue
    29
  • 47. Sample Collection Service
    • Current ongoing project:
    1. Collect 400 FFPE lung cancer samples, and 200 matched serum samples
    Perform FFPE tissue and circulating DNA isolation
    Perform real time PCR with Roche L480
    2. Collect pregnant women plasma from 100 donors and perform circulating DNA isolation
    3. Collection 4 types of matched paired frozen tumor tissue, 25-30 donors from each type of tumor
    Sample Collection
    Sample Analysis
    Sample Preparation
  • 48. IHC Service
    CD 29 Negative Control
    L0061
    L0039
    L0055
    L0039 has stronger expression of CD 29. Due to our OCT blocks were made for micro dissection RNA quality, we have quick frozen procedure, which possibly generates ice crystal. However, the staining is clearly showed and negative control is listed as well.
    31
  • 49. HMGA1 Is Induced by Wnt/β-Catenin Pathway and Maintains Cell Proliferation in Gastric Cancer Shin-ichi Akaboshi, et al. Am. J. Pathol., Oct 2009; 175: 1675 - 1685.
    Up-regulation of C-Terminal Tensin-like Molecule Promotes the Tumorigenicity of Colon Cancer through β-Catenin Yi-Chun Liao, et al. Cancer Res., Jun 2009; 69: 4563 - 4566.
    HMGA2 Maintains Oncogenic RAS-Induced Epithelial-Mesenchymal Transition in Human Pancreatic Cancer Cells Sugiko Watanabe, et al. Am. J. Pathol., Mar 2009; 174: 854 - 868.
    MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activityLifeng Liu, et al. J. Biol. Chem., Feb 2009; 284: 5165 - 5174.
    Genetic and Epigenetic SLC18A2 Silencing in Prostate Cancer Is an Independent Adverse Predictor of Biochemical Recurrence after Radical ProstatectomyKarina Dalsgaard Sørensen, et al. Clin. Cancer Res., Feb 2009; 15: 1400 - 1410.
    References
  • 50. Summary
    • A leading provider of thousands of biological samples and integrated solutions.
    • 51. 16 years of growing company experience
    • 52. Highly trained scientists
    • 53. Global business with the advantages of both US and China
    • 54. Quality, cost, and reliability
  • Conclusion
    BioChain can provide reliable and cost-effective reagents for sequencing sample preparation
    BioChain has solid experiences for OEM reagents for HTS equipment companies
    High quality and excellent service
    34
  • 55. Thank you!
    35