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  • 1. Graduate Council New, Modified or Deleted Course Form This form has many drop-down menus. To activate the menu, click on the “Choose an item.” and click the arrow to the right. 1. Please complete this form and save the file as the subject and course number without spaces (ex: PSYC648). 2. Send it as an email attachment to Jennifer Bazaz (jbazaz@gmu.edu); remember to add any related documents (for new courses, please include the catalog description with catalog credit format, e.g. 3:2:1 and the course syllabus). 3. Bring the signed original of this form to the Graduate Council meeting for our files. We will forward it on to the Registrar’s Office for implementation. -FOR COURSE CHANGES, PLEASE ONLY LIST THE MODIFICATIONS BEING MADE- ___________________________________________________________________________________________ Request for a: Modified Course Unit: COS/CHSS/KI Department: Neurosciences Subject and Course Number (if applicable, include cross listing): NEUR701 Course Title: Neurophysiology Laboratory Effective Term: (new/modified courses only): Spring, 2010 Final Term (deleted courses only):       Credit Hours: (Fixed) 3 OR (Var.)       to       For Labs or RCT:       or       Grade Type: Regular graduate (A, B, C, etc.) Repeat Status: NR Only use RD or RT when the course can be repeated for credit. Total number of hours allowable for this course to be taken (must complete for RD or RT courses):       Schedule Type Code(s): First code: LAB Second code (only for courses w/LAB or RCT component): Prerequisite or Corequisite: Prerequisite NEUR602 Please Note: For modified courses - review prerequisite or corequisite for necessary changes. For deleted courses - review other courses to correct prerequisites that list the deleted course. Description of Modification (if applicable- please be specific!): Change from 2 credits to 3 credits. Originally approved as 2 credits because we had planned 6 lab contact hours. However, it is taught at 1 hour lecture, with homework, and 5 hours lab, and it has extensive outside work for the students. Thus, it should be 3 credits. Special Instructions (major/college/class code restrictions, if applicable):       Submitted by/Contact Person: Kim Blackwell __________________________________________________________________________________________________ Signatures: Department/Unit Approval: ____________________________________________Date:       Grad Council Member: ________________________________________________Date:       Provost Office: ______________________________________________________Grad Council Approval Date: Please type into the chart those units outside of your own that may be impacted by this: N/A - Each of these units must approve this change prior to it being submitted to the Grad Council for approval.- UNIT NAME UNIT APPROVER’S NAME UNIT APPROVER’S SIGNATURE DATE                                                
  • 2. Catalog Description Hands on training in current techniques of modern neurophysiology. The course (1) acquaints the student with the theoretical basis of each technique, (2) trains the student in the laboratory skills necessary to perform each technique. Includes both intracellular and extracellular recording techniques. Preparations include both vertebrates and invertebrates. Meets once weekly for 6 hours. Open to candidates in the neuroscience Ph.D. program. •Course Designation: NEUR 701 (3:1:5) •Credit Hours: 3 •Prerequisite: Neur 602 JUSTIFICATION Course Objectives: To provide hands on training and experience in current techniques of modern neurophysiology. The course (1) acquaints the student with the theoretical basis of each technique, (2) trains the student in the laboratory skills necessary to perform each technique. Includes both intracellular and extracellular recording techniques. This course also teaches students how to keep a proper laboratory notebook. Course Necessity: This course provides an in depth introduction to current techniques of modern neurophysiology. This is the only required laboratory course for PhD neuroscience students, and thus is essential as an introduction to experimental research. It provides initial training for students whose dissertation research will be in the area of neurophysiology; it provides deep understanding and appreciation of technique for students whose dissertation research will involve modeling the results of neurophysiology research; it provides a solid foundation for understanding neuronal signal processing by students whose dissertation research is in the area of functional imaging. Thus, this courses is critical for all students in the neuroscience program. Course Relationship to Exiting Programs: The proposed course is a key component of the PhD Program in Neuroscience. It provides hands-on training in fundamental techniques in modern neurophysiology, and reinforces concepts learned in cellular neuroscience (NEUR602). Course Relationship to Existing Courses: There is no similar course at GMU. SCHEDULING and PROPOSED INSTRUCTORS • Time of Next Offering: Fall, 2010 Proposed Instructor: Theodore C. Dumas
  • 3. SAMPLE SYLLABUS Instructor: Dr. Theodore C. Dumas Office Location: Krasnow Institute, Room 109 Office Hours: Wednesday, 1-2 pm or by appointment Introduction Philosophy: A vast majority of what we know about human nervous system development, aging, and disease comes from physiology experiments performed using research animals. The main goal is for students to learn state-of-the-art laboratory techniques that are used in contemporary physiological neuroscience. Additionally, students perform anatomical experiments to correlate function with structure and assess behavior after a brain manipulation. Students are required to perform the experiments successfully, keep notes, and write a paper with corresponding grades for each dimension of the research. The instructor provides as little oversight as possible and there are numerous experiments that are not prefaced by demonstrations. Thus, students learn by performing the experiments themselves and by working as a group to come up with solutions to problems. This is course meets once per week for a 6-hour session. Purchase requirements: There is no lab fee. You’re welcome. However, students are required to purchase the Crawdad CD (Sinaur and Associates) and a hard bound notebook. NEUR701 Grading Typical lecture courses are designed to teach you facts and theories. This is a laboratory course, so you will be graded foremost on your practical knowledge and laboratory results. While primarily a neurophysiology lab, you will also perform anatomical and behavioral experiments. There are 5 parts to your final grade. 2 notebook evaluations, 2 exams, and a publication-format research paper. All portions are equally weighted (each 20% of the final grade). Notebook evaluations (March 4th & May 6th) You will be given a bound notebook in which you will take all of your notes and answer all written questions posed during each session. You will be graded on its content, including your answers to laboratory questions. You are required to hand in your notebook at the mid-term and final exams for evaluation. You will not receive your notebook back after the final exam, so if you want to keep the information you learn, you will need to make a copy for yourself. Exams (March 4th & May 6th) You will be tested on your practical knowledge in the middle and at the end of the term. The mid-term covers everything we have done to that point. The final covers everything that follows the mid-term. You will be expected to know how we do everything we do, everything contained on all handouts, and all lecture notes. Research paper (Due May 11th) You will use the hippocampal slice experiments from all students to write your paper. The style of the manuscript will follow that of the Journal of Neuroscience in terms of its overall organization and format. Honor Code All exams and homework assignments must follow the guidelines of the GMU Honor Code (www.gmu.edu/catalog/apolicies/#honor_system_and_code). Students may use other sources in preparing for presentations. When taking exams, no books, notes, or student interaction is allowed.
  • 4. Week Date Topic 1 1/21/20089 Animal use certification Introduction, define terms Goldman-Nernst equations Measure/mix solutions 2 1/28/2009 Coat histology slides in gelatin Make electrodes - glass, tungsten, SS bipolar 3 2/4/2009 Multiclamp amplfier tutorial, model cell tests Stimulus isolator tutorial (in saline dish) Introduction to Crawdad experiments Membrane Properties (Crawdad) 4 2/11/2009 Motor nerve anatomy and physiology (Crawdad) Muscle cell physiology and motor unit plasticity (Crawdad) 5 2/18/2009 Prepare hippocampal slices and incubate (10:30 am, Ted) Introduction to hippocampal slice recording Examples of EPSPs and Pop.Spikes Construct I/O and ISI curves (CA3 removal -cont-) Synaptic pharmacology-1 (CNQX, NMDA) 6 2/25/2009 Prepare hippocampal slices and incubate (10:30 am, Ted) Synaptic pharmacology-2 (Bicuculine, CA3 removal -cont-) Recording and Analysis of mormyrid EODs 7 3/4/2009 Midterm Exam, Notebook evaluation I Prepare hippocampal slices and incubate (12:10 pm, Ted) Introduction to sharp intracellular recording Sharp intracellular recording-1 (bridge balance, impale and hold) RMP, AP threshold, current injection, fill cell with biocytin 8 3/11/2009 Spring Break 9 3/18/2009 Prepare hippocampal slices and incubate (10:30 am, Ted) Whole-cell demo (Rebekah) Sharp intracellular recording-2 (synaptic responses) Section embedded slices from 2/18 with vibratome Fix slices and embed in gelatin 3/19/2009 Postfix embedded slices (30 minutes) - optional 10 3/25/2009 Introduction to histology 1o labeling of hippocampal slices (GluR antibody) Section embedded slices from 3/18 with vibratome 3/26/2009 2o antibody labeling 3/31/2009 1o labeling of hippocampal slices 11 4/1/2009 Fornix & striatal lesions Section gelatin-embedded hippocampal slices with vibratome 2o antibody labeling, ABC reaction, DAB stain, mount on slides 4/7/2009 1o labeling of hippocampal slices
  • 5. 12 4/8/2009 Spontaneous alternation in fornix-lesioned rats & perfusions Section gelatin-embedded hippocampal slices with vibratome 2o antibody labeling, ABC reaction, DAB stain, mount on slides 13 4/15/2009 Cryostat sectioning (1 hr per student) 4/21/2009 1o labeling of cryostat sections 14 4/22/2009 Introduction to microscopy Light microscopy for DAB in physiology sections 2o fluorescent antibody labeling 15 4/29/2009 Microscopy for fluorescent labeling Work on reports 16 5/6/2009 Final exam, Notebook evaluation I 5/11/2009 Research paper deadline