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  • 1. The Journal of Undergraduate Neuroscience Education (JUNE), Spring 2008, 6(2):A1-A3 ARTICLE The Most Engaging Neurophysiology Lab Exercise Ever Performed in an Undergraduate Laboratory Course Rita L. Neurotrophin,1 Eric Aplysia,2 & Santiago R. Anatomy1 1 Biology Department, Action Potential College, Hillock, IL 60101; 2Psychology Department, Synaptic State University, Neuron City, CA 90101. Abstract text here. 250 word limit. The font is Arial 10 point. here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 Abstract text here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 word limit. Abstract text word limit. Abstract text here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 word limit. here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 word limit. Abstract text here. 250 Abstract text here. 250 word limit. 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Abstract text here. 250 cell (RGC); retina; growth cone; anatomy; Aplysia word limit. Abstract text here. 250 word limit. Abstract text californica; long term potentiation (LTP); physiology Introduction text here. No word limit. The font is Arial 10 Introduction text here. No word limit. Introduction text here. point. Introduction text here. No word limit. Introduction No word limit. Introduction text here. No word limit. text here. No word limit. Introduction text here. No word Introduction text here. No word limit. Introduction text here. limit. Introduction text here. No word limit. Introduction text No word limit. Introduction text here. No word limit. here. No word limit. Introduction text here. No word limit. Introduction text here. No word limit. Introduction text here. Introduction text here. No word limit. Introduction text here. No word limit. Introduction text here. No word limit. No word limit. Introduction text here. No word limit. Introduction text here. No word limit. 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Introduction text here. No word limit. Introduction text here. JUNE is a publication of Faculty for Undergraduate Neuroscience (FUN) www.funjournal.org
  • 2. Neurotrophin et al. An Engaging Neurophysiology Lab Exercise A2 No word limit. Introduction text here. No word limit. Introduction text here. No word limit. Introduction text here. RESULTS No word limit. Introduction text here. Results text here. Subheadings may be used within this Introduction text here. No word limit. Introduction text section at the author’s discretion. Results & Discussion here. No word limit. Introduction text here. No word limit. section may be combined if so desired by the author(s). Introduction text here. No word limit. Introduction text here. No word limit. Results text here. Subheadings may be No word limit. Introduction text here. No word limit. used within this section at the author’s discretion. Results Introduction text here. No word limit. Introduction text here. & Discussion section may be combined if so desired by the No word limit. Introduction text here. No word limit. author(s). No word limit. Introduction text here. No word limit. Introduction text here. Results text here. Subheadings may be used within this No word limit. Introduction text here. No word limit. section at the author’s discretion. Results & Discussion Introduction text here. No word limit. Introduction text here. section may be combined if so desired by the author(s). No word limit. Introduction text here. No word limit. No word limit. Results text here. Subheadings may be Introduction text here. No word limit. Introduction text here. used within this section at the author’s discretion. Results No word limit. Introduction text here. No word limit. & Discussion section may be combined if so desired by the Introduction text here. No word limit. Introduction text here. author(s). No word limit. Results text here. Subheadings No word limit. Introduction text here. No word limit. may be used within this section at the author’s discretion. Introduction text here. No word limit. Introduction text here. Results & Discussion section may be combined if so No word limit. Introduction text here. No word limit. desired by the author(s). No word limit. Results text here. Introduction text here. No word limit. Introduction text here. Subheadings may be used within this section at the No word limit. Introduction text here. No word limit. author’s discretion. Results & Discussion section may be Introduction text here. No word limit. Introduction text here. combined if so desired by the author(s). No word limit. No word limit. Introduction text here. Results text here. Subheadings may be used within this section at the author’s discretion. Results & Discussion MATERIALS AND METHODS section may be combined if so desired by the author(s). Materials and methods text here if appropriate – not all No word limit. Results text here. Subheadings may be papers will need a methods section. Subheadings may be used within this section at the author’s discretion. Results used within this section at the author’s discretion. & Discussion section may be combined if so desired by the Suppliers of all equipment and reagents should be clearly author(s). No word limit. Results text here. Subheadings specified. The font is Arial 10 point. Materials and methods may be used within this section at the author’s discretion. text here if appropriate – not all papers will need a methods Results & Discussion section may be combined if so section. Subheadings may be used within this section at desired by the author(s). No word limit. Results text here. the author’s discretion. Suppliers of all equipment and Subheadings may be used within this section at the reagents should be clearly specified. Materials and author’s discretion. Results & Discussion section may be methods text here if appropriate – not all papers will need a combined if so desired by the author(s). No word limit. methods section. Subheadings may be used within this Results text here. Subheadings may be used within this section at the author’s discretion. Suppliers of all section at the author’s discretion. Results & Discussion equipment and reagents should be clearly specified. section may be combined if so desired by the author(s). Materials and methods text here if appropriate – not all No word limit. papers will need a methods section. Subheadings may be used within this section at the author’s discretion. Suppliers of all equipment and reagents should be clearly specified. Materials and methods text here if appropriate – not all papers will need a methods section. Subheadings may be used within this section at the author’s discretion. Suppliers of all equipment and reagents should be clearly specified. Materials and methods text here if appropriate – not all papers will need a methods section. Subheadings may be used within this section at the author’s discretion. Suppliers of all equipment and reagents should be clearly Figure 1. Sample Single Column Figure. Legend text goes specified. Materials and methods text here if appropriate – here. Legend text goes here. Legend text goes here. Legend not all papers will need a methods section. Subheadings text goes here. Legend text goes here. Legend text goes here. may be used within this section at the author’s discretion. Legend text goes here. Legend text goes here. Suppliers of all equipment and reagents should be clearly specified. DISCUSSION Materials and methods text here if appropriate – not all Discussion text here. Subheadings may be used within papers will need a methods section. Subheadings may be this section at the author’s discretion. Results & used within this section at the author’s discretion. Suppliers of all equipment and reagents should be clearly specified.
  • 3. The Journal of Undergraduate Neuroscience Education (JUNE), Spring 2008, 6(2):A1-A4 A3 Sample of Two-Column Table or Figure. Text should be Arial 9 pt Table 1. Sample Two-Column Table or Figure. Legend text goes here. Legend text goes here. Legend text goes here. Legend text goes here. Legend text goes here. Legend text goes here. Legend text goes here. Legend text goes here. Discussion section may be combined if so desired by the author’s discretion. Results & Discussion section may be author(s). No word limit. Discussion text here. combined if so desired by the author(s). No word limit. Subheadings may be used within this section at the Discussion text here. Subheadings may be used within author’s discretion. The font is Arial 10 point. Results & this section at the author’s discretion. Results & Discussion section may be combined if so desired by the Discussion section may be combined if so desired by the author(s). No word limit. Discussion text here. author(s). No word limit. Discussion text here. Subheadings may be used within this section at the Subheadings may be used within this section at the author’s discretion. Results & Discussion section may be author’s discretion. Results & Discussion section may be combined if so desired by the author(s). No word limit. combined if so desired by the author(s). No word limit. Discussion text here. Subheadings may be used within Discussion text here. Subheadings may be used within this section at the author’s discretion. Results & this section at the author’s discretion. Results & Discussion section may be combined if so desired by the Discussion section may be combined if so desired by the author(s). No word limit. author(s). No word limit. Discussion text here. Discussion text here. Subheadings may be used within Subheadings may be used within this section at the this section at the author’s discretion. Results & author’s discretion. Results & Discussion section may be Discussion section may be combined if so desired by the combined if so desired by the author(s). No word limit. author(s). No word limit. Discussion text here. Discussion section may be combined if so desired by Subheadings may be used within this section at the the author(s). No word limit. Discussion text here. author’s discretion. Results & Discussion section may be Subheadings may be used within this section at the combined if so desired by the author(s). No word limit. author’s discretion. Results & Discussion section may be Discussion text here. Subheadings may be used within combined if so desired by the author(s). No word limit. this section at the author’s discretion. Results & Discussion text here. Subheadings may be used within Discussion section may be combined if so desired by the this section at the author’s discretion. Results & author(s). No word limit. Discussion text here. Discussion section may be combined if so desired by the Subheadings may be used within this section at the author(s). No word limit. Discussion text here. author’s discretion. Results & Discussion section may be Subheadings may be used within this section at the combined if so desired by the author(s). No word limit. author’s discretion. Results & Discussion section may be Discussion text here. Subheadings may be used within combined if so desired by the author(s). No word limit. this section at the author’s discretion. Results & Discussion text here. Subheadings may be used within Discussion section may be combined if so desired by the this section at the author’s discretion. Results & author(s). No word limit. Discussion text here. Discussion section may be combined if so desired by the Subheadings may be used within this section at the author(s). No word limit. Discussion text here. author’s discretion. Results & Discussion section may be Subheadings may be used within this section at the combined if so desired by the author(s). No word limit. author’s discretion. Results & Discussion section may be Discussion text here. Subheadings may be used within combined if so desired by the author(s). No word limit. this section at the author’s discretion. Results & Discussion section may be combined if so desired by the REFERENCES author(s). No word limit. Discussion text here. Keller R (1991) Early embryonic development of Xenopus laevis. Subheadings may be used within this section at the In Xenopus laevis: Practical Uses in Cell and Molecular Biology author’s discretion. Results & Discussion section may be (Kay BK, Peng HB, eds) pp 102-116. San Diego, CA: Academic combined if so desired by the author(s). No word limit. Press. Discussion text here. Subheadings may be used within Lewin GR, Barde YA (1996) Physiology of the neurotrophins. this section at the author’s discretion. Results & Annu Rev Neurosci 19:289-317. McFarlane S (2000) Dendritic morphogenesis: building an arbor. Discussion section may be combined if so desired by the Mol Neurobiol 22:1-9. author(s). No word limit. Discussion text here. Nieuwkoop PD, Faber J (1967) Normal table of Xenopus Subheadings may be used within this section at the development. Amsterdam, Holland: Elsevier.
  • 4. Neurotrophin et al. An Engaging Neurophysiology Lab Exercise A4 Riddle DR, Lo DC, Katz LC (1995) NT-4-mediated rescue of lateral geniculate neurons from effects of monocular deprivation. Nature 378: 189-91. Received Month, ##, 200#; revised Month, ##, 200#; accepted Month, ##, 200#. This work was supported by The Association for the Development of Undergraduate Neuroscience Education (SRA & RLN), The Endowment for Science Education (EA), and The Synaptic State Faculty Research Foundation (EA). The authors thank Mr. Spine L. Cord, Dr. Amy G. Dala, and the students in Neuroscience 101 for technical assistance, execution, and feedback on this lab exercise. Address correspondence to: Dr. Rita L. Neurotrophin, Biology Department, 123 Growth Cone Avenue, Action Potential College, Hillock, IL 60101 Email: rln@apc.edu Rdritic rborization is temporally sensitive RGC dendritic arborization is temporally to increased retinal BDNF levels To determine if RGCs sensitive to increased retinal .BDNF levels. To RGC axonto enhanced retinal BDNF in a stage-dependent Tectal BDNF were sensitive to enhanced retinal BDNF in a were sensitive arborization dynamics are determine if RGCs retrogradely enhances RGC Retinal BDNF of alteredRGCdetermine iftreatedBDNFcontrol fashion, the effects inhibits RGC levels or stage-dependentby limits tectal dendritic onRGC dendritic arborizationBNDF dendriticonwithcoupled unaffected retinae Xenopus retinae were tectal RGC retinal retinal neurotrophins Testing Xenopus fashion, were treatedneurotrophins BDNF To with control Copyright © 2008 Faculty for Undergraduate Neuroscience microspheres from inshowed that orretinal 42 to fashion arborization To determine arborization. stage arborizationstage tectal BDNF influencesarborizationa dose-dependentinfluencedRGCto or BDNF RGC dendritic 38 toretina stage BDNF45. or stage 42To Testing the effects Todetermine ifincreasingthe retina, on levels dendriticcoupled microspheres45 ifretinal BDNF influenced RGC Our previous studiesof altered retinalwithin38 to 45 All RGC in the from neurotrophins tadpoles www.funjournal.org Control-,arborization insensitive togreen fluorescentretinal BDNF ora morphological measures of dendritic treated with ifrevealed a stage- 45. All anti-BDNF, or BDNF-treated the concentration of exogenous axonal morphologicalarborization. Toarborizationcontrol, BDNF,were promotes if RGCs are the tectum dendritic arborization, measures of dendritic arborization revealed determineRGC axon microspheres determine microspheres received tectal injections microspheres treated with control, BDNF, injectedBDNF, function-blockingBNDF.a EarlierEarlierof dendritic ng/ml Control-,into function-blocking antibodiesdistance, tadpoles injected dependent RGC axontostage 38 were microinjected withdendriticto in stage-dependent response to38tadpoles, were intraocularly were were influences the tectum of retinal retinal or anti-BDNF Xenopus retinae antibodies.the exposure 1-100 retinal anti-BDNF, or BDNF-treated green at Microsphere-containing anti-BDNF responseofarborization at BNDF.theonset microspheres retina stage tadpoles,fluorescent exposure at onset of to