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  revision  2014 revision 2014 Presentation Transcript

  • PRACTICAL REVISION
  • Scheme for slides With background Without background Brown Blue Red Organism Gram +ve cocci Organism Gram -ve
  • Schemes for slides With background Without background Gram positive cocci Gram positive bacilli Gram negative AND MIXTURES
  • Staphylococci in culture
  • Staphylococci in culture
  • Staphylococci in pus
  • Staphylococci in Pus
  • Streptococci in culture
  • Streptococci in culture
  • Streptococci in Pus
  • Streptococci in pus
  • S. pyogenes Streptococci in pus
  • Pneumococci in tissue
  • Pneumococci in tissue
  • Pneumococci in tissue
  • Quellung reaction (Streptococcus pneumoniae)
  • Neisseria in culture
  • Pathogenic Neisseria in pus
  • Pathogenic Neisseria in pus
  • Pathogenic Neisseria in pus
  • Pathogenic Neisseria in pus
  • Corynebacterium diphtheriae (Gram stain)
  • Corynebacterium diphtheriae (M.B. stain)
  • Corynebacterium diphtheriae (M.B. stain)
  • Diphtheroids
  • Bacillus anthracis (Gram stain)
  • Bacillus anthracis (Gram stain)
  • B. anthracis (Spore stain)
  • B. anthracis (Spore stain)
  • B. anthracis McFadyean reaction(polychrome MB stain)
  • Clostridium tetani
  • Clostridium tetani
  • Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  • Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  • Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  • Mycobacterium leprae (Acid Fast bacilli with modified Z-N stain)
  • Mycobacterium leprae (Acid Fast bacilli with modified Z-N stain)
  • Gram negative bacilli
  • Klebsiella in Tissues
  • Vibrio
  • Vibrio
  • Vibrio
  • Gram negative bacilli & Staphylococci Mixture
  • Gram negative bacilli & Staphylococci Mixture
  • Spirochaetes (Dark-field microscopy)
  • Spirochaetes (Fontana stain)
  • Spirochaetes (Fontana stain)
  • Spirochaetes (fontana stain)
  • Vincent’s angina
  • Candida
  • Candida
  • Mixture: Candida & Gram –ve bacilli
  • Mixture: Candida & Gram –ve bacilli
  • C. albicans (Positive Germ Tube test)
  • C. albicans (Positive Germ Tube test)
  • Nutrient agar
  • Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
  • Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
  • Pseudomonas aeruginosa on nutrient agar(showing greenish exopigment)
  • Proteus on nutrient agar (showing swarming growth)
  • Proteus on nutrient agar (showing swarming growth)
  • Mannitol salt agar
  • Mannitol salt agar
  • Mannitol salt agar
  • Blood agar
  • Types of haemolysis on Blood agar
  • Alpha-haemolysis on blood agar
  • Beta-haemolysis on blood agar
  • No-haemolysis on blood agar
  • Bacitracin test Streptococi other than group A
  • Bacitracin test Streptococci other than group A (Resistant) group A streptococci (Sensitive)
  • Optochin test
  • Optochin test Sensitive (Pneumococci) Resistant (Viridans streptococci)
  • Optochin test (sensitive) Pneumococci
  • Optochin test (resistant) Viridans streptococci
  • Corynebacterium diphtheriae on Blood Tellurite
  • Corynebacterium diphtheriae on Blood Tellurite
  • Loffler’s serum for C. diphtheriae
  • Chocolate agar for Neisseria and Haemophilus
  • Haemophilus or Neisseria on chocolate agar
  • Haemophilus influenzae on blood agar showing satellatism (growth around staphylococcus culture)
  • Haemophilus influenzae (growth only around both factors X & V) xv
  • Lowenstein Jensen medium for M. tuberculosis
  • Mycobacterium tuberculosis on Lowenstein Jensen medium
  • M. tuberculosis on L.J. medium
  • Mycobacterium Growth Indicator Tube (MGIT) (Positive tubes 1, 5 & 6) 1 2 3 4 5 6
  • Mycobacterium Growth Indicator Tube (MGIT) Positive fluorescenceNegative fluorescence
  • MacConkey’s medium
  • Lactose & Non-lactose fermenter on MacConkey’s medium
  • Lactose fermenter on MacConkey’s medium
  • Lactose fermenters on MacConkey’s medium
  • Klebsiella (mucoid & LF) on MacConkey’s medium
  • Lactose non-fermenter on MacConkey’s medium
  • Lactose non-fermenter on MacConkey’s medium
  • LNF on MacConkey’s medium
  • Salmonella on Salmonella-Shigella (S-S) agar
  • T.C.B.S. medium
  • Vibrio cholerae on T.C.B.S.
  • Blood culture technique 1 2 35 – 10 ml blood Added to 50 – 100 ml fluid medium Subculture on Solid medium
  • Candle Jar ( 5 – 10% CO2)
  • Candle Jar ( 5 – 10% CO2)
  • Cooked meat medium for anaerobic cultivation
  • Anaerobic Jar
  • Anaerobic Jar
  • Triple sugar iron( TSI ) TSI Lactose Fermenter + gas e.g. E. coli, Klebsiella Glucose Fermenter e.g.Shigella, Salmonella Glucose Fermenter + H2S e.g.Salmonella Non-fermenter e.g. Pseudomonas
  • TSI Lf +gas e.g. E.coli,Kleb NL e.g. Ps TSI ggGl fer e.g.Sal, Shig gl-ferm+H2S e.g. Salmonella
  • TSI Non fermenter e.g. Pseudomonas Lactose ferm + gas e.g. E. coli, Klebsiella Glucose fermenter e.g.Shigella, Salmonella Glucose fermenter + H2S e.g.Salmonella Glucose Fermenter +H2S e.g.Salmonella
  • Urease test -ve +ve e.g. Proteus
  • Catalase Test e.g Staphylococci e.g. Streptococci
  • Catalase Test e.g Staphylococci e.g. Streptococci
  • Coagulase Test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  • Coagulase test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  • Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
  • Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
  • Oxidase test + ve - ve Oxidase positive e.g. Neisseria, Pseudomonas
  • Indole test - ve e.g. Klebsiella + ve e.g. E.coli
  • MR & VP tests MR test VP test -ve +ve
  • Citrate test
  • B.R. of E. coli I M V C + + - - TSI MR VPIndole Citrate
  • B.R. of Klebsiella I M V C - - + + TSI VP
  • Nagler’s Reaction (Clostridium perfringens) Antitoxin No antitoxin
  • Nagler’s Reaction (Clostridium perfringens)
  • Analytical Profile Index (API) Biochemical identification system
  • Analytical Profile Index (API) Biochemical identification system
  • Analytical Profile Index (API) Biochemical identification system
  • String Test (Positive in Vibrio cholerae)
  • String Test (Positive in Vibrio cholerae)
  • Tube agglutination test (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control
  • Brucella tube agglutination test showing prozone phenomenon (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control
  • Widal Test (Recent infection with S. typhi) (Titre:1/320) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  • Widal Test (Old infection with S. typhi) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  • Widal Test (Post vaccination) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  • Latex Agglutination Test (A: Positive)
  • Latex agglutination Test (2 & 4: Positive )
  • Single radial immunodiffusion
  • Single radial immunodiffusion
  • Elek’s Test Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae
  • Elek’s Test
  • Immunofluorescence
  • Immunofluorescence
  • ELISA plate
  • ELISA plate
  • Tuberculin Skin Test (TST) Positive
  • Antibiotic sensitivity (Disc diffusion) 1 2 3 6 5 4 The organism is sensitive to 4, 5 & 6 Resistant to 1, 2 & 3
  • Antibiotic sensitivity (Disc diffusion) Sensitive to………… Resistant to………..
  • E-Test (MIC determination)
  • E-Test (MIC determination)
  • Phage-typing 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 The organism is sensitive to phages 4, 5, 6,……..etc.
  • Gel Electrophoresis DNA Marker 1 2 3 4 5 6
  • Gel Electrophoresis
  • Gel Electrophoresis
  • Western Blot For diagnosis of HIV
  • Sterile Cotton Swab
  • Sterile cup for collection of urine & sputum specimens
  • Sterile Gloves For surgery & invasive procedures
  • Non- Sterile Gloves For skin protection against blood & body fluids
  • Heavy-Duty Gloves For environmental cleaning
  • Standard mask Used for protection against droplet infections
  • N95 mask Used for protection against airborne infections e.g, M.tuberculosis
  • For protection against splashes of blood or body fluids Aprons and Gowns
  • Eye Goggles For protection against splashes of blood or body fluids
  • Rota virus (wheel-like appearance)
  • Rabies virus (bullet-shaped)
  • Coronavirus (crown-like surface projection)
  • Tissue culture bottle for cultivation of viruses
  • Plaques in a cell-monolayer
  • Examples of data show Exams
  • Choose a test
  • Choose and name the test used to detect microbial antigen. a b c d a: Slide agglutination test
  • Choose a medium
  • Choose & name the selective medium used for isolation of Mycobacterium tuberculosis. (A) (B) (C) (D) D: Lowenstein-Jensen medium
  • Infection Control
  • Infection control The most important PPE when dealing with patients having diarrhoea is: 173 a. c. b. d. d: Non-sterile gloves
  • Final Practical Examination
  • Final Practical Examination NO Mobile Phones are allowed in the department (waiting rooms & exam. halls)
  • Practical exam. : Total 43 marks Composed of 2 parts: • Part 1: 13 marks • Part 2: 30 marks
  • Part 1: Demonstration: Data show (total 13 Marks) Formed of:  3 different photos (3 marks/ 1 mark each) ANY photo included in the modified revision CD (version 2014)  2 Choose a test  2 Choose a medium  1 Infection control  5 Photos
  • Part 2: Demonstrations (total 30 Marks) Formed of 15 different stations (2 marks each) In case of a) microscopical slides: mention organism + stain b) plain culture media: mention name + use c) inoculated culture media: mention name + the organism/effect d) biochemical reactions: mention result + organism e) serological test: mention name + result/titer f) others: mention name + reading (e.g. phage-typing)
  • Name: Date: No: Microbiology Practical Exam • 1)……………………………………………………………………………………………………………………….. • 2)……………………………………………………………………………………………………………………….. • 3)……………………………………………………………………………………………………………………….. • 4)……………………………………………………………………………………………………………………….. • 5)……………………………………………………………………………………………………………………….. • 6)……………………………………………………………………………………………………………………….. • 7)……………………………………………………………………………………………………………………….. • 8)……………………………………………………………………………………………………………………….. • 9)……………………………………………………………………………………………………………………….. • 10)……………………………………………………………………………………………………………………… • 11)……………………………………………………………………………………………………………………… • 12)……………………………………………………………………………………………………………………… • 13)……………………………………………………………………………………………………………………… • 14)……………………………………………………………………………………………………………………… • 15)……………………………………………………………………………………………………………………… ………………………………………………………………………………………………………………………….. 30 Marks (2 marks each)