revision 2014

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revision 2014

  1. 1. PRACTICAL REVISION
  2. 2. Scheme for slides With background Without background Brown Blue Red Organism Gram +ve cocci Organism Gram -ve
  3. 3. Schemes for slides With background Without background Gram positive cocci Gram positive bacilli Gram negative AND MIXTURES
  4. 4. Staphylococci in culture
  5. 5. Staphylococci in culture
  6. 6. Staphylococci in pus
  7. 7. Staphylococci in Pus
  8. 8. Streptococci in culture
  9. 9. Streptococci in culture
  10. 10. Streptococci in Pus
  11. 11. Streptococci in pus
  12. 12. S. pyogenes Streptococci in pus
  13. 13. Pneumococci in tissue
  14. 14. Pneumococci in tissue
  15. 15. Pneumococci in tissue
  16. 16. Quellung reaction (Streptococcus pneumoniae)
  17. 17. Neisseria in culture
  18. 18. Pathogenic Neisseria in pus
  19. 19. Pathogenic Neisseria in pus
  20. 20. Pathogenic Neisseria in pus
  21. 21. Pathogenic Neisseria in pus
  22. 22. Corynebacterium diphtheriae (Gram stain)
  23. 23. Corynebacterium diphtheriae (M.B. stain)
  24. 24. Corynebacterium diphtheriae (M.B. stain)
  25. 25. Diphtheroids
  26. 26. Bacillus anthracis (Gram stain)
  27. 27. Bacillus anthracis (Gram stain)
  28. 28. B. anthracis (Spore stain)
  29. 29. B. anthracis (Spore stain)
  30. 30. B. anthracis McFadyean reaction(polychrome MB stain)
  31. 31. Clostridium tetani
  32. 32. Clostridium tetani
  33. 33. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  34. 34. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  35. 35. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
  36. 36. Mycobacterium leprae (Acid Fast bacilli with modified Z-N stain)
  37. 37. Mycobacterium leprae (Acid Fast bacilli with modified Z-N stain)
  38. 38. Gram negative bacilli
  39. 39. Klebsiella in Tissues
  40. 40. Vibrio
  41. 41. Vibrio
  42. 42. Vibrio
  43. 43. Gram negative bacilli & Staphylococci Mixture
  44. 44. Gram negative bacilli & Staphylococci Mixture
  45. 45. Spirochaetes (Dark-field microscopy)
  46. 46. Spirochaetes (Fontana stain)
  47. 47. Spirochaetes (Fontana stain)
  48. 48. Spirochaetes (fontana stain)
  49. 49. Vincent’s angina
  50. 50. Candida
  51. 51. Candida
  52. 52. Mixture: Candida & Gram –ve bacilli
  53. 53. Mixture: Candida & Gram –ve bacilli
  54. 54. C. albicans (Positive Germ Tube test)
  55. 55. C. albicans (Positive Germ Tube test)
  56. 56. Nutrient agar
  57. 57. Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
  58. 58. Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
  59. 59. Pseudomonas aeruginosa on nutrient agar(showing greenish exopigment)
  60. 60. Proteus on nutrient agar (showing swarming growth)
  61. 61. Proteus on nutrient agar (showing swarming growth)
  62. 62. Mannitol salt agar
  63. 63. Mannitol salt agar
  64. 64. Mannitol salt agar
  65. 65. Blood agar
  66. 66. Types of haemolysis on Blood agar
  67. 67. Alpha-haemolysis on blood agar
  68. 68. Beta-haemolysis on blood agar
  69. 69. No-haemolysis on blood agar
  70. 70. Bacitracin test Streptococi other than group A
  71. 71. Bacitracin test Streptococci other than group A (Resistant) group A streptococci (Sensitive)
  72. 72. Optochin test
  73. 73. Optochin test Sensitive (Pneumococci) Resistant (Viridans streptococci)
  74. 74. Optochin test (sensitive) Pneumococci
  75. 75. Optochin test (resistant) Viridans streptococci
  76. 76. Corynebacterium diphtheriae on Blood Tellurite
  77. 77. Corynebacterium diphtheriae on Blood Tellurite
  78. 78. Loffler’s serum for C. diphtheriae
  79. 79. Chocolate agar for Neisseria and Haemophilus
  80. 80. Haemophilus or Neisseria on chocolate agar
  81. 81. Haemophilus influenzae on blood agar showing satellatism (growth around staphylococcus culture)
  82. 82. Haemophilus influenzae (growth only around both factors X & V) xv
  83. 83. Lowenstein Jensen medium for M. tuberculosis
  84. 84. Mycobacterium tuberculosis on Lowenstein Jensen medium
  85. 85. M. tuberculosis on L.J. medium
  86. 86. Mycobacterium Growth Indicator Tube (MGIT) (Positive tubes 1, 5 & 6) 1 2 3 4 5 6
  87. 87. Mycobacterium Growth Indicator Tube (MGIT) Positive fluorescenceNegative fluorescence
  88. 88. MacConkey’s medium
  89. 89. Lactose & Non-lactose fermenter on MacConkey’s medium
  90. 90. Lactose fermenter on MacConkey’s medium
  91. 91. Lactose fermenters on MacConkey’s medium
  92. 92. Klebsiella (mucoid & LF) on MacConkey’s medium
  93. 93. Lactose non-fermenter on MacConkey’s medium
  94. 94. Lactose non-fermenter on MacConkey’s medium
  95. 95. LNF on MacConkey’s medium
  96. 96. Salmonella on Salmonella-Shigella (S-S) agar
  97. 97. T.C.B.S. medium
  98. 98. Vibrio cholerae on T.C.B.S.
  99. 99. Blood culture technique 1 2 35 – 10 ml blood Added to 50 – 100 ml fluid medium Subculture on Solid medium
  100. 100. Candle Jar ( 5 – 10% CO2)
  101. 101. Candle Jar ( 5 – 10% CO2)
  102. 102. Cooked meat medium for anaerobic cultivation
  103. 103. Anaerobic Jar
  104. 104. Anaerobic Jar
  105. 105. Triple sugar iron( TSI ) TSI Lactose Fermenter + gas e.g. E. coli, Klebsiella Glucose Fermenter e.g.Shigella, Salmonella Glucose Fermenter + H2S e.g.Salmonella Non-fermenter e.g. Pseudomonas
  106. 106. TSI Lf +gas e.g. E.coli,Kleb NL e.g. Ps TSI ggGl fer e.g.Sal, Shig gl-ferm+H2S e.g. Salmonella
  107. 107. TSI Non fermenter e.g. Pseudomonas Lactose ferm + gas e.g. E. coli, Klebsiella Glucose fermenter e.g.Shigella, Salmonella Glucose fermenter + H2S e.g.Salmonella Glucose Fermenter +H2S e.g.Salmonella
  108. 108. Urease test -ve +ve e.g. Proteus
  109. 109. Catalase Test e.g Staphylococci e.g. Streptococci
  110. 110. Catalase Test e.g Staphylococci e.g. Streptococci
  111. 111. Coagulase Test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  112. 112. Coagulase test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
  113. 113. Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
  114. 114. Oxidase test Oxidase positive e.g. Neisseria, Pseudomonas
  115. 115. Oxidase test + ve - ve Oxidase positive e.g. Neisseria, Pseudomonas
  116. 116. Indole test - ve e.g. Klebsiella + ve e.g. E.coli
  117. 117. MR & VP tests MR test VP test -ve +ve
  118. 118. Citrate test
  119. 119. B.R. of E. coli I M V C + + - - TSI MR VPIndole Citrate
  120. 120. B.R. of Klebsiella I M V C - - + + TSI VP
  121. 121. Nagler’s Reaction (Clostridium perfringens) Antitoxin No antitoxin
  122. 122. Nagler’s Reaction (Clostridium perfringens)
  123. 123. Analytical Profile Index (API) Biochemical identification system
  124. 124. Analytical Profile Index (API) Biochemical identification system
  125. 125. Analytical Profile Index (API) Biochemical identification system
  126. 126. String Test (Positive in Vibrio cholerae)
  127. 127. String Test (Positive in Vibrio cholerae)
  128. 128. Tube agglutination test (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control
  129. 129. Brucella tube agglutination test showing prozone phenomenon (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control
  130. 130. Widal Test (Recent infection with S. typhi) (Titre:1/320) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  131. 131. Widal Test (Old infection with S. typhi) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  132. 132. Widal Test (Post vaccination) O H C T C PA C PB C 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 H H
  133. 133. Latex Agglutination Test (A: Positive)
  134. 134. Latex agglutination Test (2 & 4: Positive )
  135. 135. Single radial immunodiffusion
  136. 136. Single radial immunodiffusion
  137. 137. Elek’s Test Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae
  138. 138. Elek’s Test
  139. 139. Immunofluorescence
  140. 140. Immunofluorescence
  141. 141. ELISA plate
  142. 142. ELISA plate
  143. 143. Tuberculin Skin Test (TST) Positive
  144. 144. Antibiotic sensitivity (Disc diffusion) 1 2 3 6 5 4 The organism is sensitive to 4, 5 & 6 Resistant to 1, 2 & 3
  145. 145. Antibiotic sensitivity (Disc diffusion) Sensitive to………… Resistant to………..
  146. 146. E-Test (MIC determination)
  147. 147. E-Test (MIC determination)
  148. 148. Phage-typing 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 The organism is sensitive to phages 4, 5, 6,……..etc.
  149. 149. Gel Electrophoresis DNA Marker 1 2 3 4 5 6
  150. 150. Gel Electrophoresis
  151. 151. Gel Electrophoresis
  152. 152. Western Blot For diagnosis of HIV
  153. 153. Sterile Cotton Swab
  154. 154. Sterile cup for collection of urine & sputum specimens
  155. 155. Sterile Gloves For surgery & invasive procedures
  156. 156. Non- Sterile Gloves For skin protection against blood & body fluids
  157. 157. Heavy-Duty Gloves For environmental cleaning
  158. 158. Standard mask Used for protection against droplet infections
  159. 159. N95 mask Used for protection against airborne infections e.g, M.tuberculosis
  160. 160. For protection against splashes of blood or body fluids Aprons and Gowns
  161. 161. Eye Goggles For protection against splashes of blood or body fluids
  162. 162. Rota virus (wheel-like appearance)
  163. 163. Rabies virus (bullet-shaped)
  164. 164. Coronavirus (crown-like surface projection)
  165. 165. Tissue culture bottle for cultivation of viruses
  166. 166. Plaques in a cell-monolayer
  167. 167. Examples of data show Exams
  168. 168. Choose a test
  169. 169. Choose and name the test used to detect microbial antigen. a b c d a: Slide agglutination test
  170. 170. Choose a medium
  171. 171. Choose & name the selective medium used for isolation of Mycobacterium tuberculosis. (A) (B) (C) (D) D: Lowenstein-Jensen medium
  172. 172. Infection Control
  173. 173. Infection control The most important PPE when dealing with patients having diarrhoea is: 173 a. c. b. d. d: Non-sterile gloves
  174. 174. Final Practical Examination
  175. 175. Final Practical Examination NO Mobile Phones are allowed in the department (waiting rooms & exam. halls)
  176. 176. Practical exam. : Total 43 marks Composed of 2 parts: • Part 1: 13 marks • Part 2: 30 marks
  177. 177. Part 1: Demonstration: Data show (total 13 Marks) Formed of:  3 different photos (3 marks/ 1 mark each) ANY photo included in the modified revision CD (version 2014)  2 Choose a test  2 Choose a medium  1 Infection control  5 Photos
  178. 178. Part 2: Demonstrations (total 30 Marks) Formed of 15 different stations (2 marks each) In case of a) microscopical slides: mention organism + stain b) plain culture media: mention name + use c) inoculated culture media: mention name + the organism/effect d) biochemical reactions: mention result + organism e) serological test: mention name + result/titer f) others: mention name + reading (e.g. phage-typing)
  179. 179. Name: Date: No: Microbiology Practical Exam • 1)……………………………………………………………………………………………………………………….. • 2)……………………………………………………………………………………………………………………….. • 3)……………………………………………………………………………………………………………………….. • 4)……………………………………………………………………………………………………………………….. • 5)……………………………………………………………………………………………………………………….. • 6)……………………………………………………………………………………………………………………….. • 7)……………………………………………………………………………………………………………………….. • 8)……………………………………………………………………………………………………………………….. • 9)……………………………………………………………………………………………………………………….. • 10)……………………………………………………………………………………………………………………… • 11)……………………………………………………………………………………………………………………… • 12)……………………………………………………………………………………………………………………… • 13)……………………………………………………………………………………………………………………… • 14)……………………………………………………………………………………………………………………… • 15)……………………………………………………………………………………………………………………… ………………………………………………………………………………………………………………………….. 30 Marks (2 marks each)

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