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Capnocytophaga in periodontics ppt Capnocytophaga in periodontics ppt Presentation Transcript

  • GOOD MORNING…..
  • Presented by Dr Guru Ram IIyr P.G Department of Periodontics
  • CONTENTS             INTRODUCTION GENERAL CHARACTERISTICS VARIOUS SPECIES OF CAPNOCYTOPHAGA CULTURE METHODS INCIDENCE DETECTION IDENTIFICATION INTERACTIONS VIRULENCE FACTORS ANTIBIOTIC THERAPY AND RESISTANCE CONCLUSION REFERENCES
  • INTRODUCTION
  • TERMINOLOGY Aerobic organisms  Anaerobic organisms  Micro-aerophilic organisms  Capnophilic organisms  Facultative anaerobic organisms  Obligate aerobes  Sacrolytic organisms 
  • CAPNOCYTOPHAGA  Brunner et al 1990  Green complex
  • SCIENTIFIC CLASSIFICATION Kingdom:  Phylum:  Class:  Order:  Family:  Genus:  Bacteria Bacteroidetes Flavobacteria Flavobacteriaceae Flavobacteriaceae Capnocytophaga
  • GENERAL CHARACTERISTICS Anaerobic  Gram negative  Facultative  Fusiform bacilli  Gliding movement 
  • VARIOUS SPECIES OF CAPNOCYTOPHAGA Capnocytophaga ochracea  Capnocytophaga gingivalis  Capnocytophaga sputigena  Capnocytophaga hemolytica  Capnocytophaga granulosa  Capnocytophaga canimorsus  Capnocytophaga cynodegmi 
  • Capnocytophaga ochracea Capnocytophaga sputigena
  • CAPNOCYTOPHAGA GINGIVALIS  Saccharolytic enzymes Glycosidases, galactosidases, acetyl glucosaminidase which hydrolyse the polymeric substrates
  • Capnocytophaga canimorsus
  • CULTURE METHODS Columbia agar with 5% sheep blood and standard chocolate agar Capnocytophaga sps on trypticase soya agar with horse serum Collagen or hemoglobin supplemented basal medium for C. gingivalis 
  • INCIDENCE C. g – 2.2%  Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Capnocytophaga sputigena, and Eikenella corrodens  In 1998- salari- 15.9%  Murray named only capnocytophaga sputigena as prominent bacteria in chronic periodontitis patients 
  • DETECTION  No specific methods
  • IDENTIFICATION By conventional culture methods  DNA probes Biotinylated DNA probe 
  • INTERACTIONS
  • INTERACTIONS
  • VIRULENCE FACTORS Lipo-polysaccharides  Ig A and G proteases  Gliding movement 
  • Lipopolysacharides causes Weak endotoxic activity  Capnocytophaga produces soluble products Inhibit chemotaxis of PML Prevent fibroblast proliferation 
  • THE Ig A1 AND G PROTEASES
  • ANTIBIOTIC THERAPY AND RESISTANCE     Capnocytophaga ochracea is susceptible to penicillin, ampicillin, chloramphenicol, clindamycin, tetracycline, third generation cephalosporins, quinolones and metranidazole Rummens et al. 1986 investigated the in vitro activities of antimicrobial agents against Capnocytophaga strains, Foweraker et al. 1990 studied a strain that was resistant to cephalosporins (MICs, ≥16 μg/ml). Roscoe et al. 1992 characterized a membraneassociated low-efficiency cephalosporinase that appears to be similar to the enzyme described by Foweraker et al.
  • CONCLUSION
  • REFERENCES         Micheal G Newman, Henry H Takei, Perry R Klokkevold, Fermin A Carranza. Clinical periodontology. 10th Ed.elsevier publishing Spratt DA, Greenman J, Schaffer AG. Growth and hydrolytic enzyme production of Capnocytophaga gingivalis on different protein substrates. Oral Microbiol Immunol 1999: 14: 122-126 Yacoubi Amel; Djamila Bouziane; Makhrelouf Leila and Bensoltane Advances in Medical and Dental Sciences, 3(3): 80-85, 2010. Microbiological Study of Periodontitis in the West of Algeria Sandholm L, Mahlberg K, Jousimies-Somer H. Phospholipase A1: a possible virulence factor of Capnocytophaga ochracea. Oral Microbiol Immunol 1988: 3: 18-21. Frandsen EVG, Reinholdt J, Kilian M; Immunoglobulin Al (IgAl) proteases from Prevotella (Bacteroides) and Capnocytophaga species in relation to periodontal disease. J Periodont Res 1991; 26; 297-299. Dr Arora textbook of Microbiology Betty A Forbes, Sahm Wiessfeld, Baileys and scotts, Diagnostic Microbiology. Internet sources
  • Thank You