Eric is looking to see which genes show increased expression. He is isolating RNA samples and sending them to Nikki for Next Gen Sequencing and mircoarray work. Nikki logs in to her LIMS, gets the work order on line, sets up reactions, in the system, prepares sample sheets that get downloaded to the instruments Wet lab stuff, loads samples, the instruments collect data and transfer it back to the lims Where Eric can log in and get results.
Use biological reasoning to interpret data Dinosaur DNA – is this likely?
This image shows a mouse monoclonal Ab bound to the H3 hemagluttinin from influenza virus A, strain H3N2 New sequences
AAAS talk: Bioinformatics for Biologists
Bioinformatics for Biologists www.Digital-World-Biology.com Sandra Porter Digital World Biology [email_address] Bio-Link www.bio-link.org
Bioinformatics = databases & software for working with biological information <ul><li>Acquire </li></ul>www.Digital-World-Biology.com Analyze Visualize Store
www.Digital-World-Biology.com Bioinformatics in industry Did you hear about that paper? Instrument Run Search PubMed download paper Go to GenBank download sequences Align sequences with company data Try to clone gene Design PCR primers Sequence cloned gene Make constructs for expression, sequence constructs Use microarrays / transcriptome sequencing to look at effects of construct & ligand look at structures, ligand binding
General <ul><li>Find information - in databases (>1300) </li></ul><ul><li>Analyze data </li></ul><ul><li>Prepare and interpret graphs </li></ul><ul><li>Use multiple kinds of software </li></ul><ul><li>Work with databases </li></ul><ul><li>Need practical skills for working with computers and the internet </li></ul>www.Digital-World-Biology.com