4/18/20131 Means identification of theorganisms to strain level. The main purpose to controlnosocomial infection and als...
4/18/20132Similarity between isolatesWide range of characters that could be examinedSignificance of differences needs to b...
4/18/20133 Phage is virus which live in a livingcell. Bacteriophage: is a virus live in abacterial cell, and used for ty...
4/18/20134 Phage typing of S. aureus: There are 4main group I, II, III and IV. And eachgroup consist of different number ...
4/18/20135 Bactericin is antibiotic-like substanceproduced by certain organism thatcan inhibit the growth of some closere...
4/18/20136 Advantage: Easy to perform –Standardized. Disadvantage: Cannot used forall organisms.
4/18/20137 Depend on serological reactions. Used for identification and can beused for typing. Advantage: standardized ...
4/18/20138 Also called molecular typing, eg;PCR. Advantage: very sensitive-veryspecific. Disadvantage: need special mac...
Upcoming SlideShare
Loading in...5
×

Methods for bacterial typing

2,323

Published on

0 Comments
3 Likes
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total Views
2,323
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
107
Comments
0
Likes
3
Embeds 0
No embeds

No notes for slide

Methods for bacterial typing

  1. 1. 4/18/20131 Means identification of theorganisms to strain level. The main purpose to controlnosocomial infection and also canused in different epidemiologicalpurpose (cholera, meningitis …etc).
  2. 2. 4/18/20132Similarity between isolatesWide range of characters that could be examinedSignificance of differences needs to be understoodSignificance of differences needs to be clearly expressedRelationship to other strainsComparator strains mustBe recognisable by the typing methodsHave known characteristics Phage typing. Bactericin typing. Resistotyping. Biotyping. Serotyping. Plasmid typing
  3. 3. 4/18/20133 Phage is virus which live in a livingcell. Bacteriophage: is a virus live in abacterial cell, and used for typing ofbacterial, it is specific and live incertain strain. There are two type of bacteriophage: Lytic bacteriophage: cause lysis ofbacterial cell and used for typing. Lysogenic bacteriophage: Does notcause lysis of bacterial cell but playimportant role in the gene transferring. There is a bacteriophage for anybacteria but usually used for typingof S. aurus in control of nosocomialinfections. Phage typing also used for differentepidemiological purpose as inMycobacterium, Brucella,Salmonella…etc.
  4. 4. 4/18/20134 Phage typing of S. aureus: There are 4main group I, II, III and IV. And eachgroup consist of different number ofsingle phage. Method: Reconstitute the phage (activation the virus). Inoculate the NA plate with the testedorganism after labelling. Add the phage as drop, incubate and readresult Advantage: Very specific method. Disadvantage: Difficult to perform –need well equipped laboratory(preservation of virus) – timeconsuming – not used for all type oforganisms.
  5. 5. 4/18/20135 Bactericin is antibiotic-like substanceproduced by certain organism thatcan inhibit the growth of some closerelated bacteria. Eg: Colicin typing used for typing ofShigella sonni Depend on biochemical reaction. Modified from identification totyping. Most used Analytical profile index(API). Eg: API 20 E
  6. 6. 4/18/20136 Advantage: Easy to perform –Standardized. Disadvantage: Cannot used forall organisms.
  7. 7. 4/18/20137 Depend on serological reactions. Used for identification and can beused for typing. Advantage: standardized – specific –easy to perform. Disadvantage: Cannot used for allmicroorganisms. Also called antibiogram. It is sensitivity test for typing. Used different type of antimicrobialagents differ from that used fortreatment (toxic may be dyes). Advantage: easy to perform-simple-cheap. Disadvantage: limited sensitivity(for organism highly sensitive orresistant).
  8. 8. 4/18/20138 Also called molecular typing, eg;PCR. Advantage: very sensitive-veryspecific. Disadvantage: need special machine-very expensive method.

×