PRAKTIKUM MIKROBIOLOGI S1 - FK UNAIR - INFEKSI JARINGAN DAN KULIT (INTEGUMENT) 18 - 19 MARET 2015

1. BAKTERIOLOGI INFEKSI KULIT &
JARINGAN LUNAK
DICKY BAGUS WIDHYATMOKO
18 – 19 MARET 2015
DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERAN
UNIVERSITAS AIRLANGGA

2. POKOK BAHASAN
 Staphylococcus spp
 Mycobacterium leprae

3. DISEDIAKAN
 Staphylococcus :
 S. aureus
 S. epidermidis
 S. saphrophyticus
 Mycobacterium leprae :
 slide only
 Uji Katalase
 Uji Koagulase
 Mannitol Salt Agar
(MSA)
 Novobiocin test
 MRSA Identification

4. Identifikasi Staphylococcus
 Uji Katalase
 Uji Koagulase
 Mannitol Salt Agar (MSA)
 Novobiocin test

5. Staphylococcus spp

6. Use of Catalase Test
 The catalase test is primarily used to
distinguish among Gram-positive cocci
 Staphylococcus are catalase-positive,
 members of the genera Streptococcus and
Enterococcus are catalase-negative.

7. Catalase Test
Metode :
 slide (drop) method
 tube method
 tube (slant) method
Catalase
2H2O2  O2 + 2H2O
Streptococci vs. Staphylococci

8. Catalase Test

9. Catalase POS
Staphylococcus
Catalase NEG
Catalase Test

10. Catalase Test (tube method)

11. Catalase Test (tube (slant)
method)

12. Coagulase Test
 differentiates strains of Staphylococcus aureus
from S. epidermidis and other coagulase-
negative species
 S. aureus strains are usually capable of
coagulating EDTA-treated plasma in the tube
test and will produce clumps of cells in the
slide test.

13. Coagulase Test
 method :
 SlideTest
 TubeTest

14. Coagulase Test (Slide Test)

15. S. aureus
Coagulase
Fibrinogen  Fibrin
Differential
Characteristics

16. Coagulase NEG
Coagulase POS
Staphylococcus aureus

17. Mannitol Salt Agar
 Contains :
 Mannitol
 Phenol Red as a pH indicator (pH = 7.8)
 Reaction
 Mannitol Fermented  acid produced
 pH < 6.9 : yellow color
 pH > 8.4 : red color

18. various spesies on
Tripticase Soy Agar (TSA)
A. S. aureus B. S. epidermidis C. E. coli

19. various spesies on
Tripticase Soy Agar (TSA)
A. S. aureus B. S. epidermidis C. E. coli

20. Novobiocin Test
 Differentiate between S. epidermidis w/ S. saprophyticus
 Using Novobiocin disc ( 5 g)
  < 15 mm = Resisten  S. saprophyticus

21. Identifikasi MRSA
 MRSA : Methicillin-Resistant Staphylococcus
aureus
 Methicillin  Oxacillin  Cefoxitin
 CLSI M100
 Using Cefoxitin disc :
   21 mm : MRSA
  > 21 mm : non - MRSA

22. Suspect MRSA

23. Collect Sample from wound

24. Collect Sample from nose

25. Mycobacterium Leprae
 Sample : Skin scrap using scalpel
 Using Slide with Ziehl-Neelsen Stain method
 Presence of globi (singular = globus):
encapsulated amphophilic masses of non-
viable bacilli found within macrophages or in
extracellular space

26.  Result : (Bacterial Index)
1+ At least 1 bacillus in every 100 fields.
2+ At least 1 bacillus in every 10 fields.
3+ At least 1 bacillus in every field.
4+ At least 10 bacilli in every field.
5+ At least 100 bacilli in every field.
6+ At least 1000 bacilli in every field.

27. On Hand
 Pengamatan & Pelaporan Preparat Demo
 Uji Katalase
 Kultur Media dari spesimen langsung (amies
swab) dengan :
 Blood Agar Plate
 McConkey Agar