DNA FINGERPRINTING AND FORENSIC APPLICATIONS
DNA(DEOXYRIBONUCLEIC ACID)  <ul><li>DNA is a chemical structure that forms the genes.  </li></ul><ul><li>DNA is a double h...
WHAT IS DNA FINGERPRINTING ? <ul><li>The chemical structure of everyone DNA is same but the difference lies in the base pa...
PROCESS OF DNA FINGERPRINTING <ul><li>It involves six steps </li></ul><ul><li>The process begins with the cell or sample f...
ELECTROPHORESIS
CONT…. <ul><li>4. A radioactive DNA probe is introduced .The DNA probe binds to specific DNA sequences of the nylon membra...
 
DNA  FINGERPRINTING  PROCEDURES DAY ONE
DNA  FINGERPRINTING  PROCEDURES DAY TWO
DNA  FINGERPRINTING  PROCEDURES DAY THREE
<ul><li>Applications  </li></ul><ul><li>Paternity and also the Maternity . </li></ul><ul><li>Criminal identification and F...
FORENSIC DNA  <ul><li>DNA Sequences are compared to each other. </li></ul>A G C T A G A T C G TT A TT CC G A G A G C T A G...
FORENSIC DNA (MITOCHONDRIA) <ul><li>DNA Sequences are compared to each other. </li></ul>A G C T A G A TT G TT A TT CC G A ...
FORENSIC DNA  A G C T A G A TT G TT A TT CC G A G A G C T A G A T C G TT A TT CC G A G Blood butt at crime scene Suspect #...
LET’S COMPARE……. blood stain Satheesh Dheva John Ramalingam
DO WE HAVE A MATCH? blood stain Satheesh Dheva John Ramalingam
 
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Dna Fingerprinting And Forensic Applications

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Dna Fingerprinting And Forensic Applications

  1. 1. DNA FINGERPRINTING AND FORENSIC APPLICATIONS
  2. 2. DNA(DEOXYRIBONUCLEIC ACID) <ul><li>DNA is a chemical structure that forms the genes. </li></ul><ul><li>DNA is a double helix, two strands of genetic material spiraled around each other. </li></ul><ul><li>Adenine, Guanine, Thymine ,Cytosine </li></ul>
  3. 3. WHAT IS DNA FINGERPRINTING ? <ul><li>The chemical structure of everyone DNA is same but the difference lies in the base pairs </li></ul><ul><li>Using base pairs everyone can be solely identified but it is time consuming as there are millions of base pairs </li></ul><ul><li>Instead, scientists are able to use a shorter method, because of repeating patterns in DNA </li></ul><ul><li>They are able to determine whether two DNA samples are from the same person, related people, or non-related people. </li></ul>
  4. 4. PROCESS OF DNA FINGERPRINTING <ul><li>It involves six steps </li></ul><ul><li>The process begins with the cell or sample from which the DNA is extracted. </li></ul><ul><li>The DNA is cut into fragments using restriction enzymes , The fragments are then separated into bands by electrophoresis through agarose gel . </li></ul><ul><li>The DNA band pattern is transferred into a nylon membrane . </li></ul>
  5. 5. ELECTROPHORESIS
  6. 6. CONT…. <ul><li>4. A radioactive DNA probe is introduced .The DNA probe binds to specific DNA sequences of the nylon membrane </li></ul><ul><li>5. The excess probe material is washed away leaving the unique DNA pattern </li></ul><ul><li>6. The radioactive DNA pattern is transferred to X-ray film by direct exposure .When developed the resultant visible pattern is DNA fingerprint. </li></ul>
  7. 8. DNA FINGERPRINTING PROCEDURES DAY ONE
  8. 9. DNA FINGERPRINTING PROCEDURES DAY TWO
  9. 10. DNA FINGERPRINTING PROCEDURES DAY THREE
  10. 11. <ul><li>Applications </li></ul><ul><li>Paternity and also the Maternity . </li></ul><ul><li>Criminal identification and Forensics . </li></ul><ul><li>Personal identification. </li></ul><ul><li>Problems </li></ul><ul><li>Generating a high probability . </li></ul><ul><li>Technical difficulties </li></ul>
  11. 12. FORENSIC DNA <ul><li>DNA Sequences are compared to each other. </li></ul>A G C T A G A T C G TT A TT CC G A G A G C T A G A T C G TT A TT CC G A G Hair found on Suspect Victim Conclusion: Hair may have come from the victim.
  12. 13. FORENSIC DNA (MITOCHONDRIA) <ul><li>DNA Sequences are compared to each other. </li></ul>A G C T A G A TT G TT A TT CC G A G A G C T A G A T C G TT A TT CC G A G Hair found on Suspect Victim Conclusion: Hair did not come from the victim
  13. 14. FORENSIC DNA A G C T A G A TT G TT A TT CC G A G A G C T A G A T C G TT A TT CC G A G Blood butt at crime scene Suspect #1 Conclusion: Blood could be from Suspects #2, #4 or other person with the same sequence. A G C T A G A TT G TT A TT CC G A G Suspect #2 A G C TT G A TT G TT A TT CC G A G Suspect #3 A G C T A G A TT G TT A TT CC G A G Suspect #4
  14. 15. LET’S COMPARE……. blood stain Satheesh Dheva John Ramalingam
  15. 16. DO WE HAVE A MATCH? blood stain Satheesh Dheva John Ramalingam
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