BACTERIALPHYSIOLOGY        Deepa Babin Asst Prof,Microbiology,TMC
Welcome To-------------
Meet the Microbes!
Coccus
Bacillus
Vibrio   leptospiraSpirillum   Mycoplasma
WHAT IS PHYSIOLOGY ? IT’S THEGROWTH, NUTRITION ANDMETABOLISM
Classification• Based on Nutrition bacteria are classified   as :1. Autotrophs – can synthesise all their   organic    com...
Growth Factors• Some bacteria require certain organic   compounds in minute quantities – Growth   Factors OR Bacterial Vit...
Bacterial Nutrition• Water constitutes 80% of the total weight of  bacterial cells.• Proteins, polysaccharides, lipids, nu...
Based on Nutritional Requirement• Phototrophs – Bacteria which derive  their energy from sunlight.• Chemotrophs – Bacteria...
OTHER GROWTH FACTORS• Identical with mammalian nutrition•    Vitamin B complex –1.   thiamine2.   riboflavine3.   nicotini...
GROWTH• It is an increase in all the cell  components, which ends in multiplication  of cell leading to an increase in pop...
Binary Fission
Generation time• Interval of time between two cell  divisions              OR• The time required for a bacterium to  give ...
GENERATION TIME• Coliform bacilli like E.coli & other  medically important bacteria – 20 mins• Tubercle bacilli – 20 hrs• ...
Colony – formedby         bacteriagrowing on solidmedia.(20-30 celldivisions)   Each bacterialcolony representsa clone of ...
OTHER FACTORS AFFECTING BACTERIAL            GROWTH            Temperature       Atmosphere – O2 & CO2        H-ion concen...
Temperature• Vary in their temperature requirements. • .• Temperature range – growth does not  occur above the maximum or ...
CLASSIFICATION BASED ON TEMP• Mesophilic – grows best  between 25ºC and 40ºC.  e.g. most bacterial  pathogens• Psychrophil...
OXYGEN•  Depending on the O2 requirement, bacteria   are divided into :1. Strict (Obligate) Aerobes – require O2 for   gro...
Atmosphere4.Facultative anaerobe – aerobic but can   also grow in the absence of O2e.g. Staphylococcus spps5.Aerotolerant ...
The Effect of Oxygen (O2) on Growth               Grows best in   Only grows   Grows     Grows in lowNeeds oxygen         ...
H-ion Concentration• pH range, optimum  pH• Neutral or slightly  alkaline pH (7.2 –  7.6) – majority of  pathogenic bacter...
Moisture and drying• Water – essential ingredient of bacterial  protoplasm. Hence drying is lethal to  cells.• Effect of d...
Osmotic effects• More tolerant to osmotic variation due  to mechanical strength of their cell walls.                  Radi...
BACTERIAL GROWTH CURVE• When a bacterium is added to a suitable liquid  medium & incubated, its growth follows a  definite...
PHASES OF GROWTH• Lag phase – No increase in number but  there may be an increase in the size of  the cell.• Log or Expone...
Phases• Stationary phase – cell division stops  due to depletion of nutrients &  accumulation of toxic products.  - equili...
Morphological & Physiological       alterations during growth• Lag phase – maximum cell size towards the  end of lag phase...
BACTERIAL COUNTS• Growth in numbers can be  studied by bacterial counts.• 2 methods – Total cell count            - Viable...
TOTAL COUNT• Total number of cells in the sample –   living + dead.   Can be obtained by :1. Direct counting under microsc...
Viable Cell Count•    Measures the number of living cells.•    Methods – Surface colony count1.   Dilution method2.   Plat...
Metabolism• Absorption to Elimination of substances  in cells• Oxidation-Aerobes• Fermentation-Anaerobes• Oxidation-Reduct...
Biofilms• Microbial  communities• Form slime  – quorum sensingBiofilms form onteeth, contactlenses, & catheters
THANK YOU
Bacterial physiology ppt
Bacterial physiology ppt
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Bacterial physiology ppt

  1. 1. BACTERIALPHYSIOLOGY Deepa Babin Asst Prof,Microbiology,TMC
  2. 2. Welcome To-------------
  3. 3. Meet the Microbes!
  4. 4. Coccus
  5. 5. Bacillus
  6. 6. Vibrio leptospiraSpirillum Mycoplasma
  7. 7. WHAT IS PHYSIOLOGY ? IT’S THEGROWTH, NUTRITION ANDMETABOLISM
  8. 8. Classification• Based on Nutrition bacteria are classified as :1. Autotrophs – can synthesise all their organic compounds by utilising atmospheric CO2 & N2. No medical importance.2. Heterotrophs – unable to synthesise their own metabolites & depend on preformed organic compounds. All pathogens
  9. 9. Growth Factors• Some bacteria require certain organic compounds in minute quantities – Growth Factors OR Bacterial Vitamins.• It can be :1. Essential – when growth does not occur in their absence.2. Accessory – when they enhance growth, without being absolutely necessary for it
  10. 10. Bacterial Nutrition• Water constitutes 80% of the total weight of bacterial cells.• Proteins, polysaccharides, lipids, nucleic acids, mucopeptides & low molecular weight compounds make up the remaining 20%.• For growth & multiplication, the minimum nutritional requirements are water, a source of carbon, a source of nitrogen & some inorganic salts
  11. 11. Based on Nutritional Requirement• Phototrophs – Bacteria which derive their energy from sunlight.• Chemotrophs – Bacteria which derive energy from chemical reactions.• Organotrophs : require organic sources of hydrogen• Lithotrophs : require inorganic sources of hydrogen like NH3, H2S
  12. 12. OTHER GROWTH FACTORS• Identical with mammalian nutrition• Vitamin B complex –1. thiamine2. riboflavine3. nicotinic acid4. pyridoxine5. folic acid &6. Vit.B 12
  13. 13. GROWTH• It is an increase in all the cell components, which ends in multiplication of cell leading to an increase in population.• It involves - an increase in the size of the cell & an increase in the number of individual cells.• Bacteria divide by binary fission.
  14. 14. Binary Fission
  15. 15. Generation time• Interval of time between two cell divisions OR• The time required for a bacterium to give rise to 2 daughter cells under optimum conditions• Also called population doubling time.
  16. 16. GENERATION TIME• Coliform bacilli like E.coli & other medically important bacteria – 20 mins• Tubercle bacilli – 20 hrs• Lepra bacilli – 20 days Sep 07 Dr Ekta, Microbiology, GMCA
  17. 17. Colony – formedby bacteriagrowing on solidmedia.(20-30 celldivisions) Each bacterialcolony representsa clone of cellsderived from asingle parent cell.
  18. 18. OTHER FACTORS AFFECTING BACTERIAL GROWTH Temperature Atmosphere – O2 & CO2 H-ion concentration Moisture & drying Osmotic effects Radiation Mechanical & sonic stress
  19. 19. Temperature• Vary in their temperature requirements. • .• Temperature range – growth does not occur above the maximum or below the minimum.• Optimum Temperature – growth occurs best, 37ºC for most pathogenic bacteria
  20. 20. CLASSIFICATION BASED ON TEMP• Mesophilic – grows best between 25ºC and 40ºC. e.g. most bacterial pathogens• Psychrophilic (cold loving) – grows best below 20ºC e.g. Flavobacterium spps• Thermophilic – grows best at high temp, 55- 80ºC e.g. Bacillus sterothermophilus
  21. 21. OXYGEN• Depending on the O2 requirement, bacteria are divided into :1. Strict (Obligate) Aerobes – require O2 for growth e.g. Pseudomonas aeruginosa2. Strict (Obligate) Anaerobes – grow in the absence of O2 & may even die on exposure to O2 e.g. Bacteroides fragilis3. Microaerophilic – grow best in the presence of low oxygen levels e.g. Campylobacter spp, Helicobacter spp 7
  22. 22. Atmosphere4.Facultative anaerobe – aerobic but can also grow in the absence of O2e.g. Staphylococcus spps5.Aerotolerant anaerobe – anaerobic, but tolerates exposure to O2 e.g. Clostridium perfringens6.Capnophilic organism – requires high CO2 levels eg Neisseria spps
  23. 23. The Effect of Oxygen (O2) on Growth Grows best in Only grows Grows Grows in lowNeeds oxygen oxygen, but without with or concentrations can grow oxygen without of oxygen without oxygen
  24. 24. H-ion Concentration• pH range, optimum pH• Neutral or slightly alkaline pH (7.2 – 7.6) – majority of pathogenic bacteria grow best.• Lactobacilli – acidic pH• Vibrio cholerae – alkaline pH
  25. 25. Moisture and drying• Water – essential ingredient of bacterial protoplasm. Hence drying is lethal to cells.• Effect of drying varies : T.pallidum – highly sensitive Staphylococcus spp– stand for months• Spores – resistant to dessication, may survive for several decades
  26. 26. Osmotic effects• More tolerant to osmotic variation due to mechanical strength of their cell walls. Radiation• X rays & gamma rays exposure – lethal Mechanical & Sonic Stress• May be ruptured by mechanical stress. Sep 07
  27. 27. BACTERIAL GROWTH CURVE• When a bacterium is added to a suitable liquid medium & incubated, its growth follows a definite course.• If bacterial counts are made at intervals after inoculation & plotted in relation to time, a growth curve is obtained.• Shows 4 phases : Lag, Log or Exponential, Stationary & phase of Decline.
  28. 28. PHASES OF GROWTH• Lag phase – No increase in number but there may be an increase in the size of the cell.• Log or Exponential phase – cells start dividing and their number increases exponentially.
  29. 29. Phases• Stationary phase – cell division stops due to depletion of nutrients & accumulation of toxic products. - equilibrium exists between dying cells and the newly formed cells, so viable count remains stationary• Phase of Decline – population decreases due to the death of cells – autolytic enzymes.
  30. 30. Morphological & Physiological alterations during growth• Lag phase – maximum cell size towards the end of lag phase.• Log phase – smaller cells, stain uniformly• Stationary phase – irregular staining, sporulation and production of exotoxins• Phase of Decline –involution forms(with ageing)
  31. 31. BACTERIAL COUNTS• Growth in numbers can be studied by bacterial counts.• 2 methods – Total cell count - Viable cell count
  32. 32. TOTAL COUNT• Total number of cells in the sample – living + dead. Can be obtained by :1. Direct counting under microscope using counting chambers.2. Counting in an electronic device – Coulter counter.
  33. 33. Viable Cell Count• Measures the number of living cells.• Methods – Surface colony count1. Dilution method2. Plating method• Number of colonies that develop after incubation gives an estimate of the viable count.
  34. 34. Metabolism• Absorption to Elimination of substances in cells• Oxidation-Aerobes• Fermentation-Anaerobes• Oxidation-Reduction Potential (Redox potential) - ELECTRONS TO ACCEPT OR TO PART WITH
  35. 35. Biofilms• Microbial communities• Form slime – quorum sensingBiofilms form onteeth, contactlenses, & catheters
  36. 36. THANK YOU
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