A STUDY ON INCIDENCE OFAFLATOXIGENIC FUNGI ANDAFLATOXIN CONTAMINATION IN OIL SEEDS Dr.Daxaben N. Mehta Principal Smt.Sadguna C.U.Shah Home Science and C.U.Shah Arts & Commerce Mahila college WADHWANCITY:363030
INTRODUCTIONAFLATOXIN - A Secondary Toxic Fungal MetaboliteA.flavus and A.parasiticusPotent Hepatotoxic, Carcinogenic, MutagenicPeanut and Cotton - high risk substrates
MATERIALS AND METHODSSamples of oil seeds Parts of Surendranagar district blotter test, agar plate and dilution plate methodPercentage of Incidence, Frequency and AbundanceAflatoxin producing potentials - SMKY liquid mediumCulture filtrates and oilseeds -BGYF
RESULTSIncidence of Fungi21 different fungi were isolated from thesamples of peanut, cotton, sesamum andcastor seeds. Amongst themA.niger, A. flavus, other Aspergilli,Penicillia, Rhizopus and Mucor specieswere most frequently encountered
Myco floras of Peanut seedsNine fungi isolated from peanut seedsWere A.niger, A.flavus, Aspergillus sp.,Penicillium sp., Mucor sp., Rhizopus sp.,Fusarium moniliformae, Helminthosporium sp.and Curvularia lunata.Amongst these A.niger and Aspergillus sp.were found in samples of all the localities.
Myco floras of Cotton SeedsEleven fungi isolated from cotton seedswere A.niger, A.flavus, A.tamarii, A.ochraceus,Aspergillus sp., Penicillium sp., Mucor sp.,Rhizopus sp., Fusarium moniliformae,Fusarium oxysporum, and Ustilago sp.,amongst all A. flavus and Aspergillus sp.were invariably found in all the samples
Myco floras of Castor SeedsSeven fungi isolated from castor seeds wereA.niger, A.flavus, A. ochraceus,A.candidus, Aspergillus sp., Penicillium sp.,Mucor sp. Amongst these A.niger, A. flavus,Aspergillus sp. Penicillium sp., Mucor sp.were recovered from all the samples.
Myco floras of Sesamum Seeds.Eighteen fungi isolated from sesamum seedsinclude A. niger, A. flavus, A. tamarii, A. ochraceus,Aspergillus sp., P.citrinum, P.expansum, Penicillium sp.,Mucor sp., Rhizopus sp., F.solani, F. oxysporum,F. moniliformae, Curvularia sp., Alternaria sp.,Helminthosporium sp., Rhizoctonia solani andCladosporium sp. Amongst these A.niger and A. ochrecewere recovered from all the samples.
Aflatoxigenic FungiTotal 52 isolates of A. flavus and A. parasiticuswere recovered from oil seed samplescollected from different localities. Amongst these,11 isolates were found to be toxigenic.Peanut seeds amongst all four oil seedsstudied were found to have higheraflatoxin producing potential .
Aflatoxin ContentFour different types of oil seeds collected wereanalyzed for their natural aflatoxin contamination.Peanut seeds were found to have more aflatoxincontents. Aflatoxin B1 , B2 , G1 and G2 wererecorded in peanut and sesamum seeds while onlyB1 , B2 and G1 recorded from castor seeds. Fromcotton seeds B1 along with G1 was recorded
Discussion The type of fungi and the degree of contaminationvaried with the environmental conditions. The oil seeds were more infested than oil cakes. The screening offungi from various oil seeds revealed that some fungi are toxigenic and may be a health hazard to livestockand in turn to man. Although some isolates were found to produce aflatoxin under cultural conditions, the production of aflatoxin under natural conditions is limited. This could be due to Unfavorable and changing conditionsas well as the interactions of other micro-organisms mayin turn affect the aflatoxin production.